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Integrative Zoology 2022年第6期17卷 1215-1218页

作者： michael schmitt John BUCKERIDGE Universität Greifswald Allgemeine&Systematische ZoologieGreifswaldGermany Professor Emeritus RMIT UniversityMelbourneVictoriaAustralia

Walter Joseph Bock(Fig.1)was born in Woodhaven,Queens County,New York,NY(USA),the second of 4 children of Paul and Anne Bock,née *** grew up in a German speaking family who discontinued speaking German only at the outset of *** 1947 to 1951 he attended Brooklyn Technical High School,where there was considerable emphasis on technological *** 1951,he entered Cornell University in Ithaca,NY,graduating with a Bachelor of Science in *** Cornell,he attended a short lecture series presented by the famed ethologist Konrad Lorenz,and began an association with the ornithologist Charles *** links provided Walter with a growing appreciation of the wonders of the world of biology.

关键词： teaching Walter continued

Francis Dov Por(1927–2014):A life dedicated to zoology remembered

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Integrative Zoology 2015年第2期10卷 230-231页

作者： Rosa-Maria POLYMENI John BUCKERIDGE michael schmitt Zhibin ZHANG University of Athens Greece RMIT University MelbourneVictoriaAustralia University of Greifswald Germany Institute of Zoology Chinese Academy of SciencesChina

On 25 October 2014,the International Society of Zoological Sciences(ISZS)family lost one of its most eminent members,Professor Francis Dov Por,who passed away at the age of *** was born on 25 May 1927,in Timișoara,a city that is now the capital city of Timis County and is considered the informal capital city of the historical region of Banat,*** obtained a Diploma in Biology in 1956 from the University of *** of his potential as a biologist was demonstrated through his early appointment as a Curator for the Grigore Antipa Museum of Natural History in Bucharest.

关键词： remember capital Fra

Multimodal nonlinear endomicroscopic imaging probe using a double-core double-clad fiber and focus-combining micro-optical concept

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Light(Science & Applications) 2021年第11期10卷 2173-2183页

作者： Ekaterina Pshenay-Severin Hyeonsoo Bae Karl Reichwald Gregor Matz Jörg Bierlich Jens Kobelke Adrian Lorenz Anka Schwuchow Tobias Meyer-Zedler michael schmitt Bernhard Messerschmidt Juergen Popp GRINTECH GmbH Schillerstr.107745JenaGermany Leibniz Institute of Photonic Technology Member of Leibniz Health TechnologiesAlbert-Einstein-Str.907745JenaGermany Institute of Physical Chemistry and Abbe Center of Photonics Friedrich Schiller University JenaHelmholtzweg 407743JenaGermany

Multimodal non-linear microscopy combining coherent anti-Stokes Raman scattering,second harmonic generation,and two-photon excited fluorescence has proved to be a versatile and powerful tool enabling the label-free investigation of tissue structure,molecular composition,and correlation with function and disease *** a routine medical application,the implementation of this approach into an in vivo imaging endoscope is ***,this is a difficult task due to the requirements of a multicolour ultrashort laser delivery from a compact and robust laser source through a fiber with low losses and temporal synchronization,the efficient signal collection in epi-direction,the need for small-diameter but highly corrected endomicroobjectives of high numerical aperture and compact ***,we introduce an ultra-compact fiber-scanning endoscope platform for multimodal non-linear endomicroscopy in combination with a compact four-wave mixing based fiber *** heart of this fiber-scanning endoscope is an in-house custom-designed,single mode,double clad,double core pure silica fiber in combination with a 2.4 mm diameter NIR-dual-waveband corrected endomicroscopic objective of 0.55 numerical aperture and 180µm field of view for non-linear imaging,allowing a background free,low-loss,high peak power laser delivery,and an efficient signal collection in backward direction.A linear diffractive optical grating overlays pump and Stokes laser foci across the full field of view,such that diffraction-limited performance is demonstrated for tissue imaging at one frame per second with sub-micron spatial resolution and at a high transmission of 65%from the laser to the specimen using a distal resonant fiber scanner.

关键词： fiber double scanner

Tumor antigen and MHC expression in glioma cells for immunotherapeutic interventions

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World Journal of Immunology 2013年第3期3卷 62-67页

作者： Christina Susanne Mullins Alexander Walter michael schmitt Carl-Friedrich Classen michael Linnebacher Pediatrics Department University Hospital Rostock 18057 Rostock Germany Department of General Surgery Molecular Oncology and Immunotherapy University Hospital Rostock 18057 Rostock Germany Department Internal Medicine V University Hospital Heidelberg 69120 Heidelberg Germany

AIM: To investigate the expression of tumor-antigens and major histocompatibility complex(MHC)-machinery components in glioblastoma multiforme cell lines flow cytometry staining methods were ***: Ten GBM cell lines(three commercially available: U-87 MG, U-138-MG and GMS-10 as well as seven newly established cell lines from individual patients in low-passages: HROG02, HROG04, HROG05, HROG06, HROG10, HROG13 and HROG17) were analyzed for expression of(Ⅰ) general and(Ⅱ) GBMrelated tumor antigens as well as of(Ⅲ) components of the MHC machinery by flow ***: All cell lines expressed MHC class?Ⅰ?with seven out of the ten being HLA-A02 positive. Four of the seven primary cell lines additionally expressedMHC class Ⅱ in a constitutive manner. Of note, after interferon gamma(IFN-γ) treatment, all seven cell lines expressed MHC class Ⅱ. The tumor associated antigens(TAA) EGFR and survivin were expressed at high levels in all cell lines; whereas MART-1, RHAMM, WT-1 and IL-13Rα were expressed by at least half of the cell lines and HER2/neu, MAGE-1 and tyrosinase were expressed only by few cell lines. However, all cell lines expressed at least two of the candidate antigens included into this ***: No obvious differences between commercially available and newly-established cell lines were observed. Thus, the latter in low-passages are interesting for(therapy-) screening and immunotherapeutic strategies.

关键词： Tumor antigens Glioblastoma multiforme Major histocompatibility complex molecules Tumor models Cell lines

Multimodal nonlinear imaging of atherosclerotic plaques differentiation of triglyceride and cholesterol deposits

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Journal of Innovative Optical Health Sciences 2014年第5期7卷 114-123页

作者： Christian Matthäus Riccardo Cicchi Tobias Meyer Annika Lattermann michael schmitt Bernd F.M.Romeike Christoph Krafft Benjamin Dietzek Bernhard R.Brehm Francesco S.Pavone Jürgen Popp Leibniz Institute of Photonic Technology(IPHT-Jena)Albert Einstein Straβe 9 0T145 JerlaGermany Institute of Physical Chemistry and Abbe Center of PhotonicsFriedrich Schiller University Jena Helmholtzweg 407743 JenaGermany National Institute of Optics National Research Council(INO-CNR)Largo E.Fermi 6-50125FlorenceItaly European Laboratory for Non-Linear Spectroscopy(LENS)University of Florence Via Nello Carrara 1-50019 Sesto Fiorentino(Firenze)Italy Clinic for Internal Medicine Jena University Hospital Friedrich-Schiller-UniversityErlanger Allee 10107747 JenaGermany Institute of Pathology Department of Neuropathology Jena University Hospital-Friedrich-Schiller-University Erlanger Allee 1010140 JenaGermany Catholic Clinic-Koblenz Intermal Medicine&Cardiology Rudolf Virchow Str.956073 KoblenzGermany

Cardiovascular diseases in general and atherothrombosis as the most common of its individualdisease entities is the leading cause of death in the developed ***,visualizationand characterization of inner arterial plaque composition is of vital diagnostic interest,especilly for the early recognition of vulnerable *** clinical techniques provide valuablemorphological information but cannot deliver information about the chemicai composition ofindividual ***,spectroscopic imaging techniques have recently drawn consider-able *** on the spectroscopic properties of the individual plaque components,as forinstance diferent types of lipids,the composition of atherosclerotic plaques can be analyzedqualitatively as well as ***,we compare the feasibility of multimodal nonlinearimaging combining,two-photon fluorescence(TPF),coherent anti-Stokes Raman scatering(CARS)and second-hamonic generation(SHG)microscopy to contrast composition and mor-phology of lipid deposits against the surrounding matrix of connective tissue with diffractionlimited spatial *** this contribution,the spatial distribution of major constituents of the arterial wall and atherosclerotic plaques like elastin,collagen,triglycerides and cholesterol can be simultaneously visualized by a combination of nonlinear imaging methods,providing a powerful label-free complement to standard histopathological methods with great potential forin vivo application.

关键词： Nonlinear microscopy Raman spectroscopy atherosclerosis.

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分散剂在乳胶漆中的应用

涂料工业 2002年第3期32卷 16-17页

作者：范晓玲 michael schmitt T.Staffel 德国贝克吉利尼化学有限公司上海代表处 200041 BK Giulini Chemie GmbH & Co.

综合实验结果和实际使用情况,讨论了涂料生产中聚磷酸盐与聚丙烯酸盐分散剂的应用情况,包括二者的配用以及在不同涂料体系中分散剂的选择.

关键词：分散剂乳胶漆应用聚磷酸盐聚丙烯酸盐

联合应用尼洛替尼和汉防己甲素逆转K562/A02细胞耐药与诱导凋亡的研究

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中国实验血液学杂志 2011年第1期19卷 28-33页

作者：崔婷允陈宝安丁家华高冲程坚鲍文仲悦娇单学赟高峰夏国华 Anita schmitt michael schmitt 东南大学医学院附属中大医院血液科江苏南京210009 Third Department of Internal Medicine University of Rostock

本研究旨在探讨联合应用尼洛替尼(nilotinib)和汉防己甲素(tetrandrine,Tet)逆转K562/A02细胞耐药与诱导凋亡的相关性及其机制。应用MTT法检测细胞敏感性,计算尼洛替尼和Tet干预后的柔红霉素(DNR)的半数抑制浓度(IC50),用流式细胞术(FCM... 详细信息

本研究旨在探讨联合应用尼洛替尼(nilotinib)和汉防己甲素(tetrandrine,Tet)逆转K562/A02细胞耐药与诱导凋亡的相关性及其机制。应用MTT法检测细胞敏感性,计算尼洛替尼和Tet干预后的柔红霉素(DNR)的半数抑制浓度(IC50),用流式细胞术(FCM)检测细胞凋亡率,RT-PCR检测bax、survivin mRNA的表达及Western blot分析BAX、SURVIVIN蛋白的表达情况。结果表明:5 nmol/L尼洛替尼或1.0μml/L Tet单独作用48小时后,DNR对K562/A02细胞的IC50分别为(5.71±0.72)mg/L和(6.52±0.43)mg/L,两药联合应用时DNR对K562/A02细胞的IC50降为(3.12±0.13)mg/L。单用尼洛替尼或Tet均能增加经DNR作用的K562/A02细胞的凋亡率,两药联合作用时凋亡率增高更明显。单用5 nmol/L尼洛替尼或1.0μml/L Tet 48小时后可上调bax mRNA及其蛋白的表达,且两药联合作用时上调幅度明显。单用5 nmol/L尼洛替尼或1.0μml/LTet48小时后可下调survivin mRNA及其蛋白表达水平,且两药联合作用时下调幅度明显。结论:K562/A02细胞对DNR耐药,单独应用尼洛替尼和Tet均可部分逆转K562/A02细胞对DNR的耐药,并可增加K562/A02细胞的凋亡率,两药联用具有明显的协同作用,其机制可能与上调bax mRNA及其蛋白的表达和下调survivin mRNA及其蛋白的表达有关。

关键词：尼洛替尼汉防己甲素 K562/A02细胞 survivin 耐药逆转

非清髓性骨髓移植诱导异基因受者小鼠免疫耐受的实验研究

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中国实验血液学杂志 2005年第6期13卷 1054-1057页

作者：陈宝安毕延智丁家华张琰高冲孙耘玉赵钢王骏程坚孙雪梅 michael schmitt Hartmut Dhner 东南大学医学院附属中大医院血液科南京市鼓楼医院 Third Department of Internal Medicine University of Ulm Robert-Koch-Str.8 D-89081 Ulm Germany

本研究通过非清髓性预处理方案联合髓腔内骨髓移植(IBMBMT)建立异基因小鼠免疫耐受模型,并探讨其诱导耐受的机理。受鼠为雌性C57BL/6(H2b,B6)小鼠,于第0天接受60Coγ线全身照射(TBI),4小时内输注雄性BALB/c(H2d)小鼠来源的骨髓细胞(BMC... 详细信息

本研究通过非清髓性预处理方案联合髓腔内骨髓移植(IBMBMT)建立异基因小鼠免疫耐受模型,并探讨其诱导耐受的机理。受鼠为雌性C57BL/6(H2b,B6)小鼠,于第0天接受60Coγ线全身照射(TBI),4小时内输注雄性BALB/c(H2d)小鼠来源的骨髓细胞(BMC),2天后腹腔注射环磷酰胺(CTX)。通过皮肤移植、混合淋巴细胞反应(MLR)检测耐受状态,并通过体外过继转移实验、IL2逆转实验等探讨免疫耐受的机制。结果显示,经骨髓移植的B6小鼠对BALB/c小鼠的皮肤移植物平均存活时间(MST)>150天,较对照组明显延长(P<0.01);骨髓移植后第90天,受鼠(黑色)表型开始呈现供鼠(白色)颜色特征。MLR结果证明,B6小鼠获得供体特异性耐受,该耐受可以被IL2逆转且可被过继转移;所有受鼠均未出现GVHD表现。结论:非清髓预处理联合髓腔内骨髓移植可以有效地诱导异基因小鼠免疫耐受,克隆无能、抑制细胞存在及嵌合体产生均参与耐受的形成。

关键词：骨髓移植非清髓性骨髓移植髓腔内骨髓移植免疫耐受皮肤移植

尼洛替尼联合5-溴汉防己甲素逆转K562/A02细胞耐药的研究

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中华血液学杂志 2010年第6期31卷 385-388页

作者：陈宝安单学赟程坚王飞丁家华高冲赵刚王雪梅许文林高峰夏国华 michael schmitt 东南大学临床医学院中大医院血液科南京210009 东南大学生物科学与医学工程学院生物电子学国家重点实验室江苏大学附属人民医院血液科 Internal Medicine University of Rostock Germany

目的探讨尼洛替尼、5-溴汉防己甲素（BrTet）及其联合应用对K562/A02细胞多药耐药的逆转作用及其机制.方法尼洛替尼、BrTet单独或联合作用于K562/A02细胞,应用MTY法检测细胞增殖抑制率、流式细胞术检测细胞凋亡率、RT-PCR检测mdr1 mRN... 详细信息

目的探讨尼洛替尼、5-溴汉防己甲素（BrTet）及其联合应用对K562/A02细胞多药耐药的逆转作用及其机制.方法尼洛替尼、BrTet单独或联合作用于K562/A02细胞,应用MTY法检测细胞增殖抑制率、流式细胞术检测细胞凋亡率、RT-PCR检测mdr1 mRNA的表达及Western blot分析P糖蛋白（P-gp）的表达的情况.结果 5 nmol/L尼洛替尼和0.5μmol/L BrTet单独使用48 h后,柔红霉素（DNR）对K562/A02细胞的IC50分别为4.52 mg/L和5.41 mg/L,联合使用后,DNR对K562/A02细胞的IC50降为2.98 mg/L.单用DNR、尼洛替尼和BrTet均不能增加K562/A02细胞凋亡率（P〉0.05）,DNR联合尼洛替尼和BrTet后细胞凋亡率明显增高.单用5 nmol/L尼洛替尼和0.5μmol/L BrTet 48 h后,K562/A02细胞mdr1 mRNA灰度值为0.48±0.04、0.64±0.01,两者合用K562/A02细胞mdr1 mRNA灰度值下降为0.35±0.04.单用5 nmol/L尼洛替尼作用48 h,P-gp的表达水平为0.61±0.05;单用0.5μmoL/L BrTet作用48 h,P-gp的表达水平为0.52 ±0.02;两者合用K562/A02细胞P-gp的表达水平降为0.44±0.03.结论单独应用尼洛替尼和BrTet均可部分逆转K562/A02细胞的耐药,机制可能与降低mdr1 mRNA和P-gp的表达及增加K562/A02细胞凋亡有关,并且两药联用具有明显的协同作用.

关键词：尼洛替尼 5-溴汉防己甲素基因,mdrl 糖蛋白类

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非对称战争与国际人道法

西安政治学院学报 2009年第6期22卷 98-102页

作者：米夏尔.N.施米特崔鹏美国海军军事学院德国乔治.马歇尔欧洲安全研究中心西安政治学院研究生管理大队陕西西安710068

一、引言在过去的十年里,'军事事务变革'的观念引起了军事思想家们的注意,'不对称'已成为当前的流行用语.但是,不对称很难表示一种本质上新的行动模式,因为在战争的某些特性方面,它试图通过战略、战术及武器来影响己方的实力(积极的不对... 详细信息

一、引言在过去的十年里,'军事事务变革'的观念引起了军事思想家们的注意,'不对称'已成为当前的流行用语.但是,不对称很难表示一种本质上新的行动模式,因为在战争的某些特性方面,它试图通过战略、战术及武器来影响己方的实力(积极的不对称),或利用敌方的弱点(消极的不对称),或二者兼有.

关键词：非对称战争国际人道法军事事务行动模式不对称思想家