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Morphological and microsatellite DNA diversity of Nigerian indigenous sheep

Journal of Animal Science and Biotechnology 2013年第1期4卷 18-33页

作者： Brilliant o Agaviezor Sunday o Peters Mufliat A Adefenwa Abdulmojeed Yakubu olufunmilayo A Adebambo michael o ozoje Christian oN Ikeobi Matthew Wheto oyeyemi o Ajayi Samuel A Amusan oludotun J Ekundayo Timothy M Sanni Moses okpeku Gbolabo o onasanya Marcos De Donato Babatunde M Ilori Kadir Kizilkaya Ikhide G Imumorin Department of Animal Breeding and Genetics University of Agriculture Department of Animal Science and Fisheries University of Port Harcourt Dept of Animal Science Cornell University Dept of Animal Science Berry College Department of Cell Biology and Genetics University of Lagos Department of Animal Science Nasarawa State University Dept of Livestock Production Niger Delta University Department of Animal Science Adnan Menderes University

Background： Sheep is important in the socio-economic lives of people around the world. It is estimated that more than half of our once common livestock breeds are now endangered. Since genetic characterization of Nigerian sheep is still lacking, we analyzed ten morphological traits on 402 animals and 15 microsatellite DNA markers in 384 animals of the 4 Nigerian sheep breeds to better understand genetic diversity for breeding management and germplasm conservation. Results： Morphological traits of Uda and Balami were significantly （P 〈 0.05） higher than Yankasa, which were both higher than West African Dwarf （WAD） sheep. Stepwise discriminant analysis showed tail length, rump height, chest girth, ear length and chest depth as the most discriminating variables for classification. Mahalanobis distances show the least differentiation between Uda and Balami and the largest between WAD and Balami sheep. While 93.3% of WAD sheep were correctly assigned to their source genetic group, 63.9% of Yankasa, 61.2% of Balami and 45.2% of Uda were classified correctly by nearest neighbour discriminant analysis. The overall high Polymorphism Information Content （PIC） of all microsatellite markers ranged from 0.751 to 0.927 supporting their use in genetic characterization. Expected heterozygosity was high for all loci （0.783 to 0.93）. Mean heterozygote deficiency across all populations （0.17] to 0.534） possibly indicate significant inbreeding （P 〈 0.05）. Mean values for FST, FIT and F^s statistics across all loci were 0.088, 0.394 and 0.336 respectively. Yankasa and Balami are the most closely related breeds （DA = 0.184） while WAD and Balami are the farthest apart breeds （DA-- 0.665）, which is coincident with distance based on morphological analysis and population structure assessed by STRUCTURE. Conclusions： These results suggest that within-breed genetic variation in Nigerian sheep is higher than between-breeds and may be a valuable tool for genetic improvement and conservation. The

关键词： Discriminant analysis Genetic distance Microsatellite DNA Morphological traits Nigerian sheep

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Molecular Diagnosis of Subclinical African Trypanosoma vivax Infection and Association with Physiological Indices and Serum Metabolites in Extensively Managed Goats in the Tropics

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open Journal of Veterinary Medicine 2013年第1期3卷 39-45页

作者： Timothy M. Sanni Gbolabo o. onasanya Mufliat A. Adefenwa Abdulmojeed Yakubu Christian o. N. Ikeobi olufunmilayo A. Adebambo Adewale o. Talabi michael o. ozoje Mathew Wheto michael I. Takeet Sunday o. Peters Marcos De Donato Bolaji N. Thomas Ikhide G. Imumorin Department of Animal Science Cornell University Ithaca USA

Trypanosomosis remains a major challenge to livestock production in much of tropical Sub-Saharan Africa, while diagnosis and treatment still depend on inefficient parasitological techniques. Endemic infections depend on animal reservoirs with subclinical parasitemia. We report molecular diagnosis of subclinical Trypanosoma vivax (T. vivax) infection using polymerase chain reaction (PCR) for the first time in Nigerian goats and associate parasite presence with gross physiological traits and serum metabolites in extensively managed Nigerian goats. PCR was used to amplify a 400 bp DNA fragment of the parasite genome in 205 goats across three geographical zones of the country. Results showed a high subclinical infection rate (SCIR) of 71.7% in the total goats examined. overall SCIRs of 71%, 75.9% and 55.6% were recorded in West African Dwarf, Red Sokoto and Sahel goats respectively, while geographical SCIRs were 71.2% (Southwest), 75% (Northwest) and 70% (Northeast). T. vivax presence had significant (P 0.05) effect on respiratory rate and is associated with higher creatinine levels in sera. Logistic regression analyses with Hosmer-Lemeshow goodness-of-fit showed that respiratory rate is the most important predictive trait for the presence of T. vivax infection (P 0.05). Goats appear to be a viable reservoir for T. vivax infection of other livestock. Molecular diagnosis of subclinical trypanosomosis using PCR could be useful for large scale epidemiological studies, early diagnosis of subclinical infection and treatment of the disease in extensively managed tropical goats.

关键词： Molecular Diagnosis Trypanosoma vivax Serum Metabolites Physiological Indices Nigeria Goats

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Phylogenetics and Molecular Divergence of Tilapia Fish (oreochromis Species) Using Mitochondrial D-Loop and Cytochrome b Regions

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American Journal of Molecular Biology 2018年第1期8卷 39-57页

作者： Ekerette Emmanuel Ekerette Ekei Victor Ikpeme ogbuagu Ugorji Udensi michael ohiokhuaobo ozoje owoidihe Monday Etukudo Anthony John Umoyen Samuel olutunde Durosaro Matthew Wheto Department of Genetics and Biotechnology University of Calabar Calabar Nigeria Department of Animal Breeding and Genetics Federal University of Agriculture Abeokuta Nigeria

Understanding the level of genetic diversity in any population is an important requisite towards strategizing measures for conservation and improvement of stocks. This study focused on the assessment of phylogenetics and molecular divergence of tilapia fish species obtained from two populations (Domita in South-South and odeda in South-West, Nigeria) using the displacement loop (D-loop) and cytochrome b region of the mitochondrial deoxyribonucleic acid (mtDNA). A total of 28 samples (15 from South-South and 13 from South-West) were used for the genetic analysis. DNA was extracted from the tissue of all the samples using Quik-gDNATM miniPrep kit. The D-loop containing the hypervariable region was sequenced for all samples from the two populations, while cytochrome b (Cyt b) region of mtDNA was only sequenced for samples from South-South population. Chromatograms of the sequences were viewed and edited using Bioedit software. Multiple sequence alignment was carried out using molecular evolutionary genetic analysis (MEGA) software before subsequent genetic analyses. Phylogenetic analysis grouped the samples into two clusters based on population. Also, when the two mitochondrial regions were pooled together, they clustered into two major groups based on mitochondrial regions. Analysis of molecular variance (AMoVA) revealed 37.32% variation within population and 62.68% variation among population with a significant fixation index of 0.627 (p 0.05). The genetic distance inferred between D-loop regions of South-South and South-West populations was 0.243. Maternal lineage analysis revealed that the origin of tilapia fish from both populations could be traced to oreochromis spirilus and oreochromis leucostictus based on mitochondrial D-loop region. The findings of this study revealed molecular divergence among the tilapia populations and may serve as pivot information for the genetic improvement of this important species.

关键词： Phylogenetics Molecular Divergence Maternal Lineage Tilapia Fish

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