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Exploring the Nicotinic Acetylcholine Receptor-associated Proteome with iTRAQ and Transgenic Mice

Genomics, Proteomics & Bioinformatics 2013年第4期11卷 207-218页

作者： Tristan D. McClure-Begley Kathy L. Stone michael J. marks Sharon R. Grady Christopher M. Colangelo Jon M. Lindstrom Marina R. Picciotto Department of Psychiatry Yale University School of MedicineNew Haven CT 06508 USA Institute for Behavioral Genetics University of ColoradoBoulder CO 80303 USA W.M. Keck Biotechnology Resource Laboratory Yale University School MedicineNew Haven CT 06509 USA Department of Psychology and Neuroscience University of ColoradoBoulder CO 80309 USA Department of Molecular Biophysics & Biochemistry Yale UniversityNew Haven CT 06520 USA Department of Neuroscience Medical School of the University of PennsylvaniaPhiladelphia PA 19104 USA

Neuronal nicotinic acetylcholine receptors （nAChRs） containing Gt4 and 132 subunits are the principal receptors in the mammalian central nervous system that bind nicotine with high affin- ity. These nAChRs are involved in nicotine dependence, mood disorders, neurodegeneration and neuroprotection. However, our understanding of the interactions between a4β2-containing （a4β2） nAChRs and other proteins remains limited. In this study, we identified proteins that inter- act with ct4β2 nAChRs in a gene-dose dependent pattern by immunopurifying β2 nAChRs from mice that differ in ct4 and β2 subunit expression and performing proteomic analysis using isobaric tags for relative and absolute quantitation （iTRAQ）. Reduced expression of either the a4 or the β2 subunit results in a correlated decline in the expression of a number of putative interacting proteins. We identified 208 proteins co-imrnunoprecipitated with these nAChRs. Furthermore, stratified lin- ear regression analysis indicated that levels of 17 proteins was correlated significantly with expres- sion of at4β2 nAChRs, including proteins involved in cytoskeletal rearrangement and calcium signaling. These findings represent the first application of quantitative proteomics to produce a β2 nAChR interactome and describe a novel technique used to discover potential targets for pharma- cological manipulation of a4β2 nAChRs and their downstream signaling mechanisms.

关键词： Nicotinic receptor Affinity purification Quantitative proteomics Transgenic mouse

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走近AIA培训国际会计师专业资格认证权威的培训

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成才与就业 2000年第9期 4-5页

作者： michael marks 国际会计资格培训中心

中国必将和国际标准、惯例接轨,当中国公司开始使用和国际公司同样的会计标准、管理惯例,国际会计技能在中国的需求就会大大增长。

关键词：专业资格会计师会计人员会计标准国际会计学 AIA

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In situ U-Pb,Sr,Nd and Hf isotopic analysis of eudialyte by LA-(MC)-ICP-MS

In situ U-Pb,Sr,Nd and Hf isotopic analysis of eudialyte by ...

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中国科学院地质与地球物理研究所第十届(2010年度)学术年会

作者： michael A.W.marks Roger H.Mitchell Gregor Markl Institut fur Geowissenschaften Eberhard-Karls-Universitat TubingenWilhelmstrasse 56D-72074 TubingenGermany Department of Geology Lakehead UniversityThunder BayOntarioCanada P7B 5E1

Eudialytes are a group of complex Na-Ca zirconosilicate minerals that generally occur in peralkaline agpaitic *** that eudialytes are easily altered and commonly contain inclusions of earlier-crystallized minerals,in situ laser ablation is considered as the most suitable method to determine their U-Pb ages,and Sr,Nd and Hf isotopic *** microprobe and LA-ICP-MS analysis of eudialytes from nepheline syenites from Ilimaussaq(Greenland),Khibiny and Lovozero(Russia),Saima(China),Tamazeght (Morocco),Kipawa and Mont Saint Hilaire(Canada),Pocos de Caldas(Brazil)and Langesund Fjord (Norway),indicate that this mineral typically has high contents of U,Pb,Nb,Ta,Zr,Hf and rare earth elements(REE). Analysis of an in-house standard eudialyte by both solution and laser ablation methods demonstrates that precise and accurate U-Pb ages can be obtained from eudialyte after correction for the common Pb content. The high Sr,Nd and Hf contents in eudialyte,coupled with the generally low Rb/Sr and Lu/Hf ratios,also permit the precise determination of in situ Sr,Nd and Hf isotopic ratios by LA-(MC)-ICP-MS methods. However,some eudialytes with Rb/Sr ratio of>0.02 and Yb/Sr>0.1,and Lu/Hf>0.08(or Yb/Hf>0.4-0.5) cannot be used for in situ Sr and Hf isotopic *** is the only mineral investigated to date for which it is possible to determine simultaneously U-Pb ages and Sr,Nd and Hf isotopic compositions.

关键词： U-Pb ages Sr,Nd and Hf isotopic ratios Eudialyte Laser ablation Agpaitic syenites

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Fabrication of sub-20 nm MoS_(2) horizontal nanowire on silicon substrates by inclusion of precursors into polystyrene-bpolyethylene oxide nanopatterns: Detailed structural investigation

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Nano Research 2024年第3期17卷 2145-2153页

作者： Tandra Ghoshal michael A.Morris School of Chemistry AMBER and CRANNTrinity College DublinDublinIrelandD02 AK60

Herein,we demonstrate the fabrication of sub-20 nm MoS_(2) horizontal nanowire arrays on silicon substrates using a self-assembled block copolymer assisted in situ inclusion *** separated long-range ordered polystyrene-b-polyethylene oxide(PS-b-PEO)block copolymer(BCP)line-space nanopatterns were achieved through thermo-solvent *** patterns produced had long-range order and domain sizes>1μ*** BCP structures were lightly etched and modified by anhydrous ethanol to facilitate insertion of molybdenum precursor within the film maintaining the parent BCP *** ordered molybdenum oxide nanowire arrays were then fabricated by ultraviolet(UV)/ozone treatment at room *** oxides were converted to sulphides by thermal evaporation at different temperatures in Ar/H_(2) environment.X-ray photoelectron spectroscopy revealed the composition and phases of the molybdenum oxide and sulphide *** mapping was performed to investigate the interfaces between the oxide and sulphide nanowires with the substrate *** formation and stability of the sulphide nanowires were studied at different *** photoluminescence and Raman properties were studied at different formation temperatures to investigate defects and estimate the number of layers.

关键词： MoS_(2) block copolymer nanowires inclusion X-ray photoelectron spectroscopy(XPS) mapping photoluminescence

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Neuronal trafficking as a key to functional recovery in immune-mediated neuropathies

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Neural Regeneration Research 2024年第11期19卷 2331-2332页

作者： Felix Kohle michael Schroeter Department of Neurology University of Cologne and University Hospital CologneCologneGermany

Immune-mediated neuropathies are rare diseases of the peripheral nervous system(PNS),substantially affecting patients’functionality and quality of *** are amenable to immunomodulatory treatments,which in many cases stabilize disease progression,but long-term deficits persist in many *** long-term deficits are particularly observed in monophasic autoimmune neuropathies like Guillain-Barrésyndrome and also in chronic variants,e.g.,chronic inflammatory demyelinating neuropathy(Fadia et al.,2019).

关键词： diseases autoimmune

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Rethinking the egg

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National Science Review 2024年第6期11卷 81-82页

作者： michael J.Benton School of Earth Sciences University of Bristol

Our image of early dinosaur eggs must change. Early dinosaur eggs are portrayed as soft-shelled, like those of modern lizards or snakes [1], and yet all the evidence is that early dinosaurs at least laid leathery-shel...

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Design of a Level-3 electric vehicle charging station using a 1-MW solar system via the distributed maximum power point tracking technique

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Clean Energy 2024年第1期8卷 23-35页

作者： Afshin Balal michael Giesselmann Department of Electrical and Computer Engineering Texas Tech UniversityLubbockTX 79409USA

Solar power is mostly influenced by solar irradiation,weather conditions,solar array mismatches and partial shading ***,before installing solar arrays,it is necessary to simulate and determine the possible power *** power point tracking is needed in order to make sure that,at any time,the maximum power will be extracted from the photovoltaic ***,maximum power point tracking is not a suitable solution for mismatches and partial shading *** overcome the drawbacks of maximum power point tracking due to mismatches and shadows,distributed maximum power point tracking is util-ized in this *** solar farm can be distributed in different ways,including one DC-DC converter per group of modules or per *** this paper,distributed maximum power point tracking per module is implemented,which has the highest *** technology is applied to electric vehicles(EVs)that can be charged with a Level 3 charging station in<1 ***,the problem is that charging an EV in<1 hour puts a lot of stress on the power grid,and there is not always enough peak power reserve in the existing power grid to charge EVs at that ***,a Level 3(fast DC)EV charging station using a solar farm by implementing distributed maximum power point tracking is utilized to address this ***,the simulation result is reported using MATLAB®,LTSPICE and the System Advisor *** results show that the proposed 1-MW solar system will provide 5 MWh of power each day,which is enough to fully charge~120 EVs each ***,the use of the proposed photovoltaic system benefits the environment by removing a huge amount of greenhouse gases and hazardous *** example,instead of supplying EVs with power from coal-fired power plants,1989 pounds of CO_(2) will be eliminated from the air per hour.

关键词： electric vehicles(EVs) distributed maximum power point tracking solar farm DC fast-charging station step-up DC-DC converter System Advisor Model economic analysis

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Transcranial photobiomodulation for the brain: a wide range of clinical applications

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Neural Regeneration Research 2024年第3期19卷 483-484页

作者： michael R.Hamblin Laser Research Centre Faculty of Health ScienceUniversity of Johannesburg-Doornfontein CampusDoornfonteinSouth Africa

Photobiomodulation therapy(PBMT)is a rapidly growing approach to the healing,stimulation,protection,and regeneration of many human organs and tissue *** started in the 1960s as low-level laser therapy for wound healing,but since then the introduction of light-emitting diodes(LEDs)has dramatically increased the number of applications and reports of positive *** generally uses red(620-700 nm)and/or near-infrared(780-1270 nm)wavelengths of light at an intensity that causes no tissue heating,and its activity is based on well-established biological and cellular mechanisms(de Freitas and Hamblin,2016).While laser therapists continue to use various types of laser in their office practice,LEDs are ideally suited for home use devices because they are completely safe and without any known significant adverse *** the various body parts on which PBMT has been shown to exert beneficial effects,the brain stands out as perhaps the most promising *** has been shown to reduce neuroinflammation,while increasing mitochondrial function,oxygen consumption,and blood flow within the brain(Hamblin,2016).Moreover,PBMT can stimulate the processes of synaptogenesis,neurogenesis,and neuroplasticity thus helping the brain to heal itself.

关键词： healing stimulation wound

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Odd-even effects in the structure and properties of aryl-substituted aliphatic self-assembled monolayers

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Nano Research 2024年第5期17卷 4231-4243页

作者： Piotr Cyganik Andreas Terfort michael Zharnikov Jagiellonian University Faculty of PhysicsAstronomy and Applied Computer ScienceSmoluchowski Institute of PhysicsŁojasiewicza 1130-348 KrakówPoland Institut für Anorganische und Analytische Chemie Universität FrankfurtMax-von-Laue-Straße 760438 FrankfurtGermany Applied Physical Chemistry Heidelberg UniversityIm Neuenheimer Feld 25369120 HeidelbergGermany

Self-assembled monolayers(SAMs)represent an important tool in context of nanofabrication and molecular engineering of surfaces and *** properties of functional SAMs depend not only on the character of the tail groups at the SAM-ambient interface,but are also largely defined by their *** its turn,the latter parameter results from a complex interplay of the structural forces and a variety of other factors,including so called odd-even effects,*** of the SAM structure and properties on the parity of the number(odd or even)of individual building blocks in the backbone of the SAM *** most impressive manifestation of the odd-even effects is the structure of aryl-substituted alkanethiolate SAMs on Au(111)and Ag(111),in which,in spite of the fact that the intermolecular interaction is mostly determined by the aryl part of the monolayers,one observes a pronounced dependence of molecular inclination and,consequently,the packing density of the SAM-forming molecules on the parity of number of methylene units in the alkyl *** we review the properties of the above systems as well as address fundamental reasons behind the odd-even effects,including the existence of a so-called bending potential,which is frequently disregarded in analysis of the structure-building *** generality of the odd-even effects in SAMs is additionally supported by the recent data for SAMs on GaAs,scanning tunneling microscopy data for SAMs on Ag(111),and the data for the monolayers with selenolate and carboxyl anchoring groups on Au(111)and Ag(111).The implications of these effects in terms of the control over the packing density and orientation of the tail groups at the SAM-ambient interface,structural perfection,polymorphism,temperature-driven phase transitions,and SAM stability toward such factors as ionizing radiation,exchange reaction,and electrochemical desorption are *** implications place the odd-even effects as an important tool

关键词： self-assembled monolayers molecular films surface and interface engineering odd-even-effects

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Increasing realism in modelling energy losses in railway vehicles and their impact to energy-efficient train control

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Railway Engineering Science 2024年第3期32卷 257-285页

作者： michael Nold Francesco Corman Institute for Transport Planning and Systems ETH ZürichZürichSwitzerland

The reduction of energy consumption is an increasingly important topic of the railway ***-efficient train control(EETC)is one solution,which refers to mathematically computing when to accelerate,which cruising speed to hold,how long one should coast over a suitable space,and when to *** approaches in literature and industry greatly simplify a lot of nonlinear effects,such that they ignore mostly the losses due to energy conversion in traction components and *** fill this research gap,a series of increasingly detailed nonlinear losses is described and *** categorize an increasing detail in this representation as four *** study the impact of those levels of detail on the energy optimal speed *** do this,a standard approach based on dynamic programming is used,given constraints on total travel *** evaluation of multiple test cases highlights the influence of the dynamic losses and the power consumption of auxiliary components on railway trajectories,also compared to multiple *** results show how the losses can make up 50%of the total energy consumption for an exemplary *** them would though result in consistent but limited errors in the optimal ***,more complex trajectories can result in less energy consumption when including the complexity of nonlinear losses than when a simpler model is *** effects are stronger when the trajectory includes many acceleration and braking phases.

关键词： Train trajectory optimization Energy-efficient train control(EETC) Dynamic efficiency Power losses in railway vehicles

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