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Evaluation of Chlorophyll Content and Fluorescence Parameters as Indicators of Drought Tolerance in Barley

Agricultural Sciences in China 2006年第10期5卷 751-757页

作者： LI Rong-hua GUO Pei-guo michael baum Stefania Grando Salvatore Ceccarelli College of Life Science Guangzhou University Guangzhou 510006 P.R.China International Center for Agricultural Research in the Dry Areas （ICARDA） P.O. Box 5466 Aleppo Syria

Drought is a major abiotic stress that severely affects food production worldwide. Agronomic and physiological traits associated with drought tolerance are suitable indicators for selection of drought tolerance genotypes to reduce the impact of water deficit on crop yield in breeding program. The objective of this study was to identify indicators related to drought tolerance through analysis of photosynthetic traits in barley （Hordeum vulgare L.）. These traits included chlorophyll content, initial fluorescence （Fo）, maximum primary yield of photochemistry of photosystem Ⅱ （Fv /Fo） and maximum quantum yield of photosystem Ⅱ （Fv/Fm）. Four genotypes （Tadmor, Arta, Morocco9-75 and WI2291） variable in drought tolerance were used to investigate the correlation between these traits and drought tolerance. The results reflected that all of these traits were affected negatively in the four genotypes at different levels of post-anthesis drought stress, but the decrease in drought tolerant genotypes was much less than that of drought sensitive genotypes. The results further revealed that the components of the photosynthetic apparatus could be damaged significantly in drought sensitive genotypes, while drought tolerant genotypes were relatively less affected. On the other hand, the values of chlorophyll content, Fo, Fv/Fo and Fv/Fm in drought tolerance genotypes were significantly higher than those in drought sensitive genotypes under drought stress. It was concluded that chlorophyll content, Fo, Fv/Fo and Fv/Fm could be considered as reliable indicators in screening barley germplasm for drought tolerance.

关键词： barley (Hordeum vulgare L.) chlorophyll chlorophyll fluorescence parameters drought tolerance

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Molecular Characterization of Atlas 66-Derived Wheat Near-Isogenic Lines Contrasting in Aluminum (Al) Tolerance

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Agricultural Sciences in China 2007年第5期6卷 522-528页

作者： GUO Pei-guo BAI Gui-hua LI Rong-hua Brett Carver michael baum College of Life Science Guangzhou University Guangzhou 510006 P.R.China USDA-ARS-PIant Science and Entomology Research Unit 4008 Throckmorton Hall Kansas State University Manhattan KS 66506 USA Department of Plant and Soil Sciences Oklahoma State University 0K74078 USA International Center for Agricultural Research in the Dry Areas （ICARDA） P.O.Box 5466 Aleppo Syria

Aluminum （Al） toxicity is the major limiting factor for wheat growth in acidic soils. Genetic improvement of Al tolerance is one of the most cost-effective solutions to improve wheat productivity. The objective of this study was to characterize near isogenic lines （NILs） contrasting in Al tolerance derived from Atlas 66 in the backgrounds of Al-sensitive cultivars Chisholm and Century using amplified fragment length polymorphism （AFLP） and simple sequence repeat （SSR）. A total of 200 AFLP and 88 SSR primer pairs were screened and 12 markers （11 AFLPs and one SSR） were associated with Al-tolerance in NILs of at least one recurrent parental background. Among them, nine were linked to A1 tolerance in the Chisholm-derived NILs, seven were associated with Al-tolerance in the Century-derived NILs, and three AFLPs derived from the primer combinations of pAG/mGCAG, pCAG/mAGC and pGTG/mGCG, and one SSR, Xwmc331 on chromosome 4D, associated with A1 tolerance in NILs of both recurrent parental backgrounds. Those common markers across two backgrounds may be the major marker loci associated with Al-tolerance in Atlas 66 and could be useful for marker-assisted breeding to improve Al tolerance in wheat. In addition, evaluation of Al tolerance among different genotypes using hematoxylin stain and relative root growth revealed that Atlas 66 was more tolerant to Al stress than the NILs, therefore suggested that the Al-tolerant NILs might not carry all Al-tolerance loci from Arias 66 and inheritance of Al tolerance in Arias 66 is more likely multigenic.

关键词： aluminum (Al) tolerance Triticum aestivum L. molecular marker

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Genetic Diversity and Association Analysis for Salinity Tolerance, Heading Date and Plant Height of Barley Germplasm Using Simple Sequence Repeat Markers

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Journal of Integrative Plant Biology 2008年第8期50卷 1004-1014页

作者： Lilia Eleuch Abderrazek Jilal Stefania Grando Salvatore Ceccarelli Maria von Korff Schmising Hisashi Tsujimoto Amara Hajer Abderrazek Daaloul michael baum Laboratory of Genetics and Plant Breeding Department of Agronomy and Plant Biotechnology National Institute of Agronomy 1085 Tunis Tunisia International Centre for Agricultural Research in the Dry Areas (ICARDA) 5466 Aleppo Syria International Centre for Agricultural Research in the Dry Areas (ICARDA) 5466 Aleppo Syria Laboratory of Plant Genetics and Breeding Science Faculty of Agriculture Tottori UniversityTottori 680-8553 Japan

The objective of this study was to investigate the genetic diversity of barley accessions. Additionally, association trait analysis was conducted for grain yield under salinity, heading date and plant height. For this purpose, 48 barley genotypes were analyzed with 22 microsatellite simple sequence repeat （SSR） markers. Four of the 22 markers （Bmac316, scssr03907, HVM67 and Bmag770） were able to differentiate all barley genotypes. Cluster and principal coordinate analysis allowed a clear grouping between countries from the same region. The genotypes used in this study have been evaluated for agronomic performance in different environments. Conducting association analysis for grain yield under salinity conditions using TASSEL software revealed a close association of the marker Bmag749 （2H, bin 13） in two different environments with common significant alleles （175, 177）, whereas the HVHOTR1 marker （2H, bin 3） was only significant in Sakhar Egypt with alleles size being 158 and 161. Heading date also showed an association with scssr03907 through the common significant specific allele 111 and EBmac0415 markers in three different agro climatic locations, whereas HVCMA, scssr00103 and HVM67 were linked to heading date in the Egyptian environment only. The plant height association analysis revealed significant markers Bmag770 via the significant allele 152 and scssr09398.

关键词： association analysis genetic variability plant height polymorphism salinity, heading date simple sequence repeat.

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Development of a panel of unigene-derived polymorphic EST–SSR markers in lentil using public database information

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The Crop Journal 2016年第5期4卷 425-433页

作者： Debjyoti Sen Gupta Peng Cheng Gaurav Sablok Dil Thavarajah Pushparajah Thavarajah Clarice J.Coyne Shiv Kumar michael baum Rebecca J.McGee Department of Plant Sciences North Dakota State University ICAR-ARS Indian Institute of Pulses Research Division of Plant Sciences University of Missouri Department of Biodiversity and Molecular Ecology Research and Innovation Centre Fondazione Edmund Mach Agricultural and Environmental Sciences Clemson University International College Beijing China Agricultural University USDA-ARS Western Regional Plant Introduction Station Washington State University BIGM Program International Center for Agricultural Research in the Dry Areas (ICARDA) USDA-ARS Grain Legume Genetics and Physiology Research Unit Washington State University

Lentil(Lens culinaris Medik.), a diploid(2n = 14) with a genome size greater than 4000 Mbp, is an important cool season food legume grown worldwide. The availability of genomic resources is limited in this crop species. The objective of this study was to develop polymorphic markers in lentil using publicly available curated expressed sequence tag information(ESTs). In this study, 9513 ESTs were downloaded from the National Center for Biotechnology Information(NCBI) database to develop unigene-based simple sequence repeat(SSR) markers. The ESTs were assembled into 4053 unigenes and then analyzed to identify 374 SSRs using the MISA microsatellite identification tool. Among the 374 SSRs, 26 compound SSRs were *** pairs for these SSRs were designed using Primer3 version 1.14. To classify the functional annotation of ESTs and EST–SSRs, BLASTx searches(using E-value 1 × 10-5) against the public UniP rot(http://***/) and NCBI(http://***/) databases were performed. Further functional annotation was performed using PLAZA(version3.0) comparative genomics and GO annotation was summarized using the Plant GO slim category. Among the synthesized 312 primers, 219 successfully amplified Lens DNA. A diverse panel of 24 Lens genotypes was used to identify polymorphic markers. A polymorphic set of 57 markers successfully discriminated the test genotypes. This set of polymorphic markers with functional annotation data could be used as molecular tools in lentil breeding.

关键词： Lens culinaris EST-SSRs Functional annotation Unigene sequences EST database Genetic resources

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应用SSR标记对61个国家大麦遗传多样性的研究

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植物遗传资源学报 2008年第1期9卷 15-19页

作者：张赤红张京赵会英张云霞李珍 michael baum 中国农业科学院作物科学研究所北京100081 国际农业干旱研究中心叙利亚阿勒颇5466

使用全自动基因分析仪（ABI-3700 DNA Analyzer），用35对SSR荧光标记引物对来自61个国家的2625份大麦种质资源的遗传多样性进行了分析，得出如下结果：（1）35对引物在2625份大麦种质资源中共检测到2063个等位变异，每个位点的等位变... 详细信息

使用全自动基因分析仪（ABI-3700 DNA Analyzer），用35对SSR荧光标记引物对来自61个国家的2625份大麦种质资源的遗传多样性进行了分析，得出如下结果：（1）35对引物在2625份大麦种质资源中共检测到2063个等位变异，每个位点的等位变异数为30～79个，平均为58.94；多样性指数为1.60～3.66，平均为2.80；（2）在大麦的7条染色体中，每条染色体的等位变异数不等，染色体等位变异数从多到少排序为5、7、2、6、3、4、1，遗传多样性指数从高到低依次为2、6、7、3、5、4、1，综合这两项指标，第2、7条染色体遗传多样性较高，第1条染色体的遗传多样性最低；（3）约旦、伊朗、叙利亚和利比亚等国家大麦的遗传多样性指数最高，分别为2.58、2.53、2.50和2.38；而埃塞俄比亚、叙利亚、伊朗和约旦等国家的大麦资源则具有较多的等位变异，分别为29.03、25.57、25.11和24.60，综合分析，约旦、伊朗、叙利亚和土耳其大麦遗传多样性较高。

关键词：大麦遗传多样性微卫星标记

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Is <sup>18</sup>F-FDG-PET/CT a Valid Non-Invasive Predictor for Regression Grade after Neoadjuvant Treatment in Patients with NSCLC Stage III?

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Journal of Cancer Therapy 2014年第6期5卷 531-541页

作者： Natalie Desiree Klass Richard P. baum michael Schmuecking Norbert Presselt Klaus-michael Mueller Thomas G. Wendt Reiner Bonnet Department of Nuclear Medicine Zentralklinik Bad Berka Bad Berka Germany Department of Pneumology Zentralklinik Bad Berka Bad Berka Germany Department of Radiation Oncology University of Jena Jena Germany Department of Radiation-Oncology Inselspital Bern University Hospital and University of Bern Bern Switzerland Department of Thoracic Surgery Zentralklinik Bad Berka Bad Berka Germany Institute of Pathology University of Münster Münster Germany

Introduction: CT alone cannot provide sufficient information referring to response after neoadjuvant therapy in a timely manner. To evaluate the role of 18F-FDG-PET after neoadjuvant chemoradiation as a valid, non-invasive predictor for early therapy response and its effect on survival as compared to histopathologic tumor response, data of 32 of 210 randomized patients with NSCLC stage IIIA/IIIB, who were treated in a?prospective randomized controlled multicenter trial (LUCAS- MD), were re-evaluated. Material and Methods: For 32 patients with NSCLC stage IIIA (44%) IIIB (56%) neoadjuvant treatment consisted of two to three cycles of chemotherapy (225 mg/m2 paclitaxel and carboplatin AUC 6 d1q22) and concomitant chemoradiation (50 mg/m2 paclitaxel and carboplatin AUC 2 d1, d8, d15;1.5 Gy b.i.d. up to 45 Gy). Documentation of involved lymph node stations as detected by 18F-FDG-PET/CT and lymph node sampling during surgery according to the IASLC lymph node mapping (2009). Evaluation of histological regression grade (RG) according to Junker et al. (2001) and correlation with?18F-FDG-PET/CT for primary tumor and each lymph node station. Calculation of disease free survival using Kaplan-Meier estimates and log rank tests. Results: Actuarial tumor specific survival for the 32 patients with concomitant chemoradiation plus?chemotherapy: complete vs. incomplete metabolic remission prior to surgery after 60 months:?40% vs. 24% (p?= 0.018). RG III/IIb (no/less than 10% of vital tumor cells) vs. RG IIa/I (more than 10% vital tumor cells) after 60 months: 46% vs. 15% (p?= 0.006). 18/32 (56%) patients had RG III/IIb, 8/32 (25%) patients had regression grade III. 1/8 pts. with RG III were in the 18F-FDG- PET/CT false positive, 10 pts. with RG IIb (i.e. all pts. with RG IIb) were in the 18F-FDG-PET/CT false negative. One patient with RG IIa was in the 18F-FDG-PET/CT false negative. Hence, the cut-off level in detecting vital tumor cells by 18F-FDG-PET/CT after neoadjuvant chemoradia

关键词： NSCLC 1818F-FDG-PET Neoadjuvant Treatment Non-Invasive Predictor

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干旱胁迫下大麦基因表达的研究

干旱胁迫下大麦基因表达的研究

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2006中国植物细胞发育与分子生物学学术研讨会

作者：郭培国李荣华 michael baum 广州大学生命科学学院广州大学生命科学学院 International Center for Agricultural Research in the Dry Areas （ICARDA） P.O.Box 5466 AleppoSyria

＜正＞干旱是一个世界范围内严重影响作物产量的自然灾害,其发生的频率和影响范围威胁着世界粮食的供给。我国也是一个受干旱严重影响的国家之一,据国家节水灌溉杨凌工程技术研究中心（http:／／***／news）报道,我国每年因干旱粮食减...

＜正＞干旱是一个世界范围内严重影响作物产量的自然灾害,其发生的频率和影响范围威胁着世界粮食的供给。我国也是一个受干旱严重影响的国家之一,据国家节水灌溉杨凌工程技术研究中心（http:／／***／news）报道,我国每年因干旱粮食减产100亿kg-250亿kg,遇到严重干旱年份,粮食减产高达500多亿kg。大麦是位于水稻、小麦和玉米之后的第四种主要禾谷类作物, 在大麦生长季节,常常遭遇到不同程度的干旱胁迫,严重地影响大麦生产。因此,认识和了解大麦抗旱的分子机理是非常有意义的一项研究工作。

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用基因芯片技术探讨大麦抗旱相关的功能基因

用基因芯片技术探讨大麦抗旱相关的功能基因

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2006年中国植物逆境生理生态与分子生物学学术研讨会

作者：郭培国李荣华 michael baum 广州大学生命科学学院广州大学生命科学学院 International Center for Agricultural Research in the Dry Areas（ICARDA） P.O．Box 5466AleppoSyria

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干旱胁迫下大麦一些光合特性QTLs的研究

干旱胁迫下大麦一些光合特性QTLs的研究

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2006中国植物细胞发育与分子生物学学术研讨会

＜正＞大麦是一个重要的禾谷类作物,在世界上的各大洲均有种植,它是干旱和半干旱地区农民可依赖于自然降雨而栽种的作物之一。但在大麦的生长季节,常常受到干旱的侵袭,严重的影响了大麦的生长。因此,种植抗旱的大麦品种是一种有效地减...

＜正＞大麦是一个重要的禾谷类作物,在世界上的各大洲均有种植,它是干旱和半干旱地区农民可依赖于自然降雨而栽种的作物之一。但在大麦的生长季节,常常受到干旱的侵袭,严重的影响了大麦的生长。因此,种植抗旱的大麦品种是一种有效地减轻干旱为害的主要措施。传统的通过表现型选育品种的方法在抗旱品种的选育上取得了不少的成绩,但由于干旱的复杂性和环境互作的影响,特别是对抗旱性状的遗传认识不足,导致抗旱品种的选育进展较慢。在最近10多年来,分子标记技术已被广泛地用来进行多种作物性状的遗传研究和分子标记辅助选择育种上。这种技术具有潜力减少环境互作的影响,提高选择的效率。

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在乳腺癌普查中临床乳腺检查可以取代乳腺X线照相吗?

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英国医学杂志中文版 2003年第1期6卷 57-59页

作者： Indraneel Mittra michael baum Hazel Thornton Joan Houghton 王万祥 Department of Surgical Oncology Tata Memorial HospitalMumbai-400012India Department of Surgery University College Medical School London WIP 7LD Saionara 31 Regent Street.RowhedgeColchester CO5 7EA CRC and UCL Cancer Trials Centre Royal Free and University College London Medical SchoolLondon WIA 8AN

乳腺癌普查和乳腺X线照相在公众的理解中已成为同义词,但事实上却不一定是一回事。乳腺癌理想的普查方法应该是既能降低乳腺癌的病死率、假阳性率,花费又要相对便宜。进行普查需要费用,但不应该以降低对有症状患者的医疗服务为代价,也... 详细信息

乳腺癌普查和乳腺X线照相在公众的理解中已成为同义词,但事实上却不一定是一回事。乳腺癌理想的普查方法应该是既能降低乳腺癌的病死率、假阳性率,花费又要相对便宜。进行普查需要费用,但不应该以降低对有症状患者的医疗服务为代价,也不应该耗费国家医疗卫生服务体系(NHS)的有限资源,以便花在同样重要的、但是公众没有特别关注的一些领域。

关键词：乳腺癌普查临床乳腺检查诊断乳腺X线照相

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