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a.modula. microfluidic pla.form t. st.dy how fluid shea. st.ess a.t.rs est.ogen recept.r phenot.pe in ER+brea.t.ca.cer cells

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Microsyst.ms & Na.oengineering 2024年第1期10卷 287-299页

作者： Bra.lio a.drés Ort.ga.Quesa.a.Jona.ha. Cuccia.Ra.ha.l Coa.es blake Na.sa. Et.a. Lit.lefield Eliza.et. C.Ma.t.n a.a. t.Melvin Ca.n Depa.t.ent.of Chemica. Engineering Louisiana State UniversityBaton RougeLA 70803USA Depa.t.ent.of Chemica. a.d Biologica. Engineering Clemson UniversityClemsonSC 29634USA Biologica. a.d a.ricult.ra. Engineering Louisiana State UniversityBaton RougeLA 70803USA Depa.t.ent.Medicine Section Hematology and Medical OncologyTulane UniversityNew OrleansLA 70118USA

Met.0}t.0}ic brea.t.ca.cer lea.s t. poor prognoses a.d worse out.omes in pa.ient. due t. it. inva.ive beha.ior a.d poor response t. *** is st.ll unclea. wha. biophysica. a.d biochemica. fa.t.rs drive t.is more a.gressive phenot.pe in met.0}t.0}ic ca.cer;however recent.st.dies ha.e suggest.d t.a. exposure t. fluid shea. st.ess in t.e va.cula.ure could ca.se *** t.is st.dy a.modula. microfluidic pla.form ca.a.le of mimicking t.e ma.nit.de of fluid shea. st.ess(FSS)found in huma. va.cula.ure wa. designed a.d *** device provides a.pla.form t. eva.ua.e t.e effect. of FSS on MCF-7 cell line,a. est.ogen recept.r posit.ve(ER+)brea.t.ca.cer cell line,during circula.ion in t.e *** of t.e effect. of FSS on MCF-7 cells wa. ca.ried out.ut.lizing t.o a.proa.hes:single cell a.a.ysis a.d bulk *** single cell a.a.ysis,cells were t.a.ped in a.microa.ra. a.t.r exit.ng t.e serpent.ne cha.nel a.d followed by immunost.0}ning on t.e device(on-chip).Bulk a.a.ysis wa. performed a.t.r cells were collect.d in a.microt.be a. t.e out.et.of t.e microfluidic serpent.ne cha.nel for west.rn blot.ing(off-chip).It.wa. found t.a. cells exposed t. a. FSS ma.nit.de of 10 dyn/cm^(2) wit. a.residence t.me of 60 s enha.ced expression of t.e prolifera.ion ma.ker Ki67 in t.e MCF-7 cell line a. a.single cell *** underst.0}d possible mecha.isms for enha.ced Ki67 expression,on-chip a.d off-chip a.a.yses were performed for pro-growt. a.d surviva. pa.hwa.s ERK,a.t.a.d Ja./*** demonst.a.ed t.a. a.t.r shea.ing t.e cells phosphoryla.ion of p-a.t.p-mt.R,a.d p-St.0}3 were ***,t.ere wa. no cha.ge in p-ERK1/*** is a.media.or of ER ra.id signa.ing,a.a.ysis of phosphoryla.ed ERαwa. ca.ried out.a.d no significa.t.differences bet.een shea.ed a.d non-shea.ed popula.ions were *** t.get.er t.ese result. demonst.a.e t.a. FSS ca. increa.e phosphoryla.ion of prot.ins a.socia.ed wit. a.more a.gressive phenot.pe in circula.ing ca.cer *** findin

关键词： fluid brea.t est.ogen

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“Doubling” of loca. phot.n emissions when t.o simult.0}eous, spa.ia.ly-sepa.a.ed, chemiluminescent.rea.t.ons sha.e t.e sa.e ma.net.c field configura.ions

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Journa. of Biophysica. Chemist.y 2012年第1期3卷 72-80页

作者： blake t. dotta michael a. persinger Biomolecula. Sciences Progra. Laurentian University Sudbury Canada

t.e a.m of t.e present.experiment. wa. t. discern if t.e “ent.0}glement.-like phot.n emissions from pa.rs of cell cult.res or huma. bra.ns sepa.a.ed by significa.t.dist.0}ces but.sha.ing t.e sa.e circling ma.net.c field could be demonst.a.ed wit. a.cla.sic chemiluminescent.rea.t.on produced by hydrogen peroxide a.d hypochlorit.. Simult.0}eous inject.on of t.e sa.e a.ount.of peroxide int. a.loca. dish (a.ove a.phot.mult.plier t.be) a.d a.dish 10 m a.a. in a.closed cha.ber produced a.“doubling” of t.e dura.ions of t.e phot.n spikes only when t.e t.o rea.t.ons were pla.ed in t.e cent.r of sepa.a.e spa.es a.ound ea.h of which ma.net.c fields were genera.ed a. a.celera.ing group velocit.es cont.0}ning decrea.ing pha.e modula.ions followed by decelera.ing group velocit.es embedded wit. increa.ing pha.e modula.ions. t.e dura.ion of t.is “ent.0}glement. wa. a.out.8 min. t.ese result. suggest.t.a. sepa.a.e dist.0}ces beha.e a. if t.ey were “t.e sa.e spa.e” if t.ey a.e exposed t. t.e sa.e precise t.mpora. configura.ion of ma.net.c fields wit. specific a.gula. velocit.es.

关键词： Phot.n Emission Ent.0}glement a.gula.ly a.celera.ing Ma.net.c Fields Hydrogen Per-oxide/Hypochlorit. Rea.t.on

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a.t.a.sient.but.Prot.a.t.d Geoma.net.c a.oma.y in t.e Sudbury Ba.in Following t.o Nea.-Cont.guous Int.nse Geoma.net.c St.rms

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Int.rna.iona. Journa. of Geosciences 2011年第3期2卷 363-365页

作者： michael a.persinger.blake t.dotta 不详

During t.e ma.nt.0}ned quiescence bet.een sola. cycle 23 a.d 24, t.o unusua.ly int.nse (K-indices = 7) globa. geoma.net.c dist.rba.ces sepa.a.ed by 6 da.s occurred. t.ey were followed by a.prot.a.t.d increa.e of bet.een 150 a.d 200 nt.in t.e vert.ca. component.of our loca. ma.net.c field (Sudbury, Ont.0}io). t.e dura.ion of t.e va.ia.ion a.oma.y wa. unusua.ly long, a.out.3 weeks, before ret.rning t. ba.eline following a.one week period of below a.era.e int.nsit. cha.a.t.rized by a.proxima.ely 50 min periodicit.es. We suggest.t.is a.oma.y support. previous resea.ch t.a. specific t.mpora. pa.t.rns of increa.ed globa. geoma.net.c a.t.vit. when ma.ched wit. loca. impeda.ce/reluct.0}ce of ore bodies crea.ed t.e condit.on for rema.ka.le t.a.sient.cha.ges in t.e surfa.e st.0}ic int.nsit. of ma.net.c fields.

关键词： a.oma.ous t.a.sient. Sudbury Ba.in Ma.net.c St.rm Periodicit.

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Seed Germina.ion a.d t.eir Phot.n Emission Profile Following Exposure t. a.Rot.0}ing Ma.net.c Field

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Open Journa. of Biophysics 2019年第4期9卷 254-266页

作者： Vict.ria.L. Hossa.k michael a. persinger.blake t. dotta Beha.ioura. Neuroscience a.d Biology Progra.s Laurentian University Sudbury Ontario Canada

a.mult.t.de of experiment. ha.e a.plied ma.net.c fields t. pla.t. or seeds a.d found a.va.iet. of different.a.d somet.mes cont.a.ict.ng result.. a.ma.net.c field genera.ing device ca.led t.e Chrysa.is resona.or ha. been shown t. influence t.e bra.n a.t.vit. of huma. pa.t.cipa.t., t.e phot.n emissions from ba.t.ria. ma.ma.ia. cell cult.res a.d wa.er. In t.is experiment.sunflower seeds (Helia.t.us a.nus) were a.lowed t. begin germina.ion a.d t.en exposed t. eit.er t.e field genera.ed by t.e Chrysa.is resona.or or a.sha. condit.on. t.eir growt. a.d phot.n emissions were t.0}en over t.e next.5 da.s. It.wa. found t.a. t.e seeds showed less germina.ion 48 hours a.t.r exposure a.d significa.t.y higher phot.n emissions when 3 seeds were mea.ured t.get.er in a.dish, but.not.if 2 seeds or 1 seed were mea.ured. t.ere were no significa.t.differences in t.e phot.n mea.urement. from t.e wa.er t.e seeds were germina.ing in. t.ese result. ma. indica.e t.a. t.e seeds beca.e more sensit.ve t. t.e presence of neighbouring seeds. t.e phot.n emissions result. were a.so significa.t.y impa.t.d by ext.rna. wea.her condit.ons.

关键词： Seed Germina.ion Phot.n Communica.ion Ma.net.c Field

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Bright.light.t.a.smit. t.rough t.e bra.n: Mea.urement.of phot.n emissions a.d frequency-dependent.modula.ion of spect.a. elect.oencepha.ogra.hic power

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World Journa. of Neuroscience 2013年第1期3卷 10-16页

作者： michael a. persinger.blake t. dotta Kevin S. Sa.oka Beha.ioura. Neuroscience Progra. Laurentian University Sudbury Canada

Phot.ns a.e emit.ed during bra.n a.t.vit. a.d when a.plied ext.rna.ly a.t.r it. funct.ona. connect.vit. during t.e rest.ng st.0}e. In t.e present.st.dy we a.plied const.0}t.or t.me va.ying light.(~10,000 lux) st.muli t. one side of t.e skull a.d mea.ured by phot.mult.plier t.bes t.e phot.n densit. emit.ed from t.e opposit. side of t.e skull a.ong it. t.o horizont.0} pla.es. Globa. qua.t.t.0}ive elect.oencepha.ogra.hic a.t.vit. (QEEG) wa. recorded simult.0}eously. Relia.le increa.es of ~2.5 × 10-11 W· m-2 during eit.er const.0}t.or specific fla.h frequencies bet.een 3 a.d 7 Hz a. well a. enha.ced QEEG power in t.e t.et.0}a.d low bet.0}ra.ge were mea.ured. a.cording t. source loca.iza.ion by Low Resolut.on Elect.oma.net.c t.mogra.hy (LOREt.0} t.e right.pa.a.ippoca.pa. region wa. pa.t.cula.ly enha.ced. Ca.cula.ions employing known qua.t.t.0}ive va.ues for permea.ilit. a.d permit.ivit. of bra.n t.ssue were consist.nt.wit. t.is frequency ba.d. Est.ma.ed concent.a.ions of prot.ns from hydronium ions indica.ed a.Grot.huss cha.n-like process for moving phot.n energy t.rough t.e cerebra. medium ma. ha.e media.ed t.e dist.0}ce-dependent.la.ency. t.e result. suggest.t.a. ext.rna. light.is t.a.smit.ed t.rough cerebra. t.ssue, ca. be mea.ured ext.rna.ly, a.d significa.t.y a.fect. funct.ona. connect.vit.. t.e findings support.t.e conclusions of St.0}ck et.a.. (World Journa. Neuroscience, 2012).

关键词： Cerebrum Int.a.erebra. Light t.a.smission Phot.n Densit. Qua.t.t.0}ive Elect.oencepha.ogra.hy (QEEG) LOREt. (Low Resolut.on Elect.oma.net.c t.mogra.hy)

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Ma.ked Increa.es in Ba.kground Phot.n Emissions in Sudbury Ont.0}io More t.a. One Week before t.e Ma.nit.de &gt.8.0 Ea.t.qua.es in Ja.a. a.d Chile

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Int.rna.iona. Journa. of Geosciences 2012年第3期3卷 627-629页

作者： michael a. persinger Ghisla.ne F. La.reniere blake t. dotta La.rent.a. Universit. Sudbury Ontario Canada P3E 2C6

Da.ly, minut.-t.-minut. mea.urement. of ground level phot.n emissions in Sudbury, Ont.0}io Ca.a.a.displa.ed conspicuous increa.es more t.a. one week before t.e 2011 M9.0 ea.t.qua.e in Ja.a. a.d t.e 2010 M8.8 ea.t.qua.e in Chile. t.mpora. profiles of t.e a.t.cedent.increa.e a.d subsequent.decline in power densit.es for t.e t.o event. were rema.ka.ly simila.. a.t.cedent.cha.ges for 7.0 8.0) seismic event. a.ywhere on t.e pla.et.

关键词： Phot.n Emissions Seismic Event. Ea.t.qua.e a.t.cedent.

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Four Yea.s of Da.ly Phot.n Emissions t.a. Ha.e Predict.d Ma.or Ea.t.qua.es: Ra. Da.a. Spect.a. Power Densit. a.a.yses a.d Implica.ions for t.e Geosciences

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Int.rna.iona. Journa. of Geosciences 2015年第4期6卷 311-316页

作者： michael a. persinger Brenda. Lehma. blake t. dotta Ghisla.ne F. La.reniere La.rent.a. Universit. Sudbury Canada

t.e da.ly media. ground surfa.e flux densit.es per da. from a.cont.nuously opera.ing phot.mult.plier t.be unit.for four yea.s in Sudbury, Ont.0}io a.e present.d for December 2010 t. December 2014. Increa.es of a.out.2 t. 4 PMt.unit. (1 unit.= 5 × 10-11 W·m-2) for media. da.ly mea.ures relia.ly occurred a.out.t.o weeks before M ≥ 7.7 ea.t.qua.es a.ywhere on t.e pla.et. t.e PMt.unit., unt.l June 2014, usua.ly ret.rned t. ba.eline wit.in a.few da.s a.t.r t.e event.. t.ere ha. been a.slow posit.ve drift.in flux power densit. since a.out.2012 a.d a.conspicuous ma.nt.0}ned increa.e a.t.r Ma., 2014. t.e equiva.ent.energy per da. if it.were represent.d isot.opica.ly wit.in t.e volume occupied by t.e ea.t. is t.e sa.e order of ma.nit.de a. t.e a.era.e da.ly globa. t.t.0} seismic energy relea.e. Spect.a. power densit.es revea.ed enha.ced pea.s (periodicit.es) bet.een 100 t. 150 da.s, 60 da.s, 30 da.s, a.d 25 da.s. Discrimina.le pea.s in power were not.d a.ound 18 da.s, 14 da.s, a.d 4 t. 6 da.s. t.ese result. suggest.t.a. cont.nuous mea.urement.of phot.nemissions wit.in hyper-da.k condit.ons ma. revea. geophysica. processes t.a. precede la.ger seismic event. a.d could reflect.t.e movement.of t.e ea.t.-sola. syst.m a.ound t.e ga.a.t.c cent.r.

关键词： Ba.kground Phot.n Emissions Globa. Seismicit. Spect.a. Power Densit.es Da.ly Mea.urement.

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Shift. in Phot.n Spect.a. Power Densit.es wit.in Schuma.n (7.7 t. 7.8 Hz) Va.ues in Microt.bules during Complex Ma.net.c Field Exposures Ma. Reflect.a. Informa.ion Int.rfa.e wit. Universa. Energies

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Open Journa. of Biophysics 2015年第3期5卷 84-95页

作者： michael a. persinger.blake t. dotta Da.id a. E. Va.es St.1}ley a. Koren Qua.t.m Neurobiology La.ora.ory Behavioural Neuroscience Biomolecular Sciences and Human Studies Programs Laurentian University Sudbury Canada

t.e specific dia.et.r of microt.bules wa. shown t. be a.prima.y solut.on when ma.net.c energy wa. set.equa. t. Ca.imir energy. t. discern if t.is spa.ia. cont.0}nment.could be foci for informa.ion phot.n emissions were mea.ured from prepa.a.ions of microt.bules (Mt.) while t.ey were exposed in sequent.a. 4 min int.rva.s t. va.ious pa.t.rns of wea. ma.net.c fields whose int.nsit.es ra.ged from 3 t. 10 μt. Ca.cula.ions from t.e media. ma.s of a.t.bulin dimer, it. summed cha.ges a.d t.e a.plied ma.net.c field a. well a. t.e cha.ge in ma.net.c moment.derived from t.e energy of t.e hydrogen line when a.plied t. our experiment.0} fields predict.d a.dyna.ic shift.(Δf) bet.een 0.03 a.d 0.21 Hz. Spect.a. power densit.es (SPD) indica.ed ma.ked enha.cement. in phot.n numbers during periods of ma.net.c field exposures wit.in t.e 7.6 t. 7.8 Hz increment. t.e t.t.0} SPD unit. for t.e shift.were 10-18 t. 10-17 J per s. Five of t.e eight.pa.t.rns elicit.d a.split.spect.um of power wit.in t.is ra.ge. Sepa.a.e fa.t.r a.a.yses of t.e SPDs of t.e seria. va.ues t.a. composed t.e point. of t.e a.t.a. field pa.t.rns indica.ed t.ose t.a. evoked t.e split.spect.um (Δf = 0.05 t. 0.13 Hz) displa.ed significa.t.y higher loa.ings on t.e sa.e fa.t.r compa.ed t. t.ose t.a. did not. If t.is shift.in phot.n energy reflect. a.pha.e modula.ion of t.e coherence frequency (8 MHz) of Mt., t.e increment.of energy per MHz frequency would be wit.in t.e energy of t.e neut.a. hydrogen line. t.ese result. suggest.t.a. t.e int.insic st.uct.re or informa.ion from specific int.nsit. ma.net.c fields when a.plied t. Mt. is reflect.d in phot.n energy densit.es va.illa.ing a.ound t.e funda.ent.0} Schuma.n Resona.ce t.a. could be a. int.rfa.e bet.een Ca.imir a.d ma.net.c sources.

关键词： Microt.bules Ca.imir Energy Ma.net.c Energy Phot.n Emissions Schuma.n Resona.ce Pha.e Modula.ion Split.Spect.um

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Ma.net.c Field Configura.ions Corresponding t. Elect.ic Field Pa.t.rns t.a. Evoke Long-t.rm Pot.nt.a.ion Shift.Power Spect.a.of Light.Emissions from Microt.bules from Non-Neura. Cells

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Open Journa. of Biophysics 2014年第4期4卷 112-118页

作者： blake t. dotta Da.id a.E. Va.es Ca.ly a. Buckner Robert.M. La.renie michael a. persinger Biomolecula. Sciences a.d Beha.ioura. Neuroscience Progra.s Laurentian University Sudbury Canada

Phot.n count. were mea.ured every 15 ms for 75 s from microt.bule-enriched prepa.a.ions (a.d nuclei) from mouse mela.oma.cells during ba.eline a.d a.t.r 2 min exposures t. 1 μt.ma.net.c fields. t.e ma.net.c fields were genera.ed from a.circula. a.ra. of solenoids a.d present.d wit. a.celera.ing or decelera.ing rot.0}ion velocit.es. t.e ra.ge of phot.n ra.ia.t.flux densit. wa. in t.e order of 10-12 W·m-2. Microt.bules prepa.a.ions t.a. ha. been exposed for only 2 min t. a.ma.net.c field configura.ion corresponding t. t.e elect.ic field pa.t.rn t.a. induced long-t.rm pot.nt.a.ion in neura. t.ssue when a.plied a. elect.ic current.displa.ed pea.s of spect.a. power densit.es wit.in 7 - 8 Hz, 9.5 Hz, 14 - 15 Hz, a.d 22 Hz ba.ds. t.e ma.or pea. (9.4 Hz) ba.dwidt. wa. a.proxima.ely 0.1 Hz. While microt.bule prepa.a.ions exposed for 2 min t. a.7 Hz sine-wa.e or in t.e a.sence of a.field emit.ed a. overa.l simila. level of spect.a. power densit., t.e pea.s in power densit. were not.present. t.ea.ment.wit. t.e Lt. pa.t.rned fields, compa.ed t. t.e ba.eline or sine-wa.e fields prima.ily a.t.red t.e frequency ba.d in which t.e a.plit.de of t.e phot.n field wa. expressed. t.ese result. suggest.t.a. t.e phot.n emissions from microt.bule prepa.a.ions ha.e t.e ca.a.it. t. respond t. specifica.ly-pa.t.rned or geomet.ic sha.es of ma.net.c fields by a.t.ring spect.a. configura.ions ra.her t.a. t.e a.solut. numbers of phot.ns.

关键词： Microt.bules Phot.n Emissions Spect.a. Densit. Shift. Physiologica.ly-Pa.t.rned Ma.net.c Fields

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La.ged Coherence of Phot.n Emissions a.d Spect.a. Power Densit.es bet.een t.e Cerebra. Hemispheres of Huma. Subject. during Rest.Condit.ons: Pha.e Shift.a.d Qua.t.m Possibilit.es

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World Journa. of Neuroscience 2016年第2期6卷 119-125页

作者： J. N. Cost.0}B. t. dotta M. a. persinger Beha.ioura. Neuroscience Progra. Laurentian University Sudbury Canada Biology Depa.t.ent Laurentian University Sudbury Canada Psychology Depa.t.ent Laurentian University Sudbury Canada Biomolecula. Sciences Progra. Laurentian University Sudbury Canada

Phot.n count. a.out.15 cm from t.e left.a.d right.sides of t.e hea. while subject. sa. quiet.y during ba.eline condit.ons wit.in a.hyper-da.k cha.ber were mea.ured by phot.mult.plier unit.. La./lea. a.a.yses for phot.n emissions bet.een t.e t.o hemispheres indica.ed a.wea. but.st.0}ist.ca.ly significa.t.correla.ion bet.een t.e a.plit.de fluct.a.ions t.a. were sepa.a.ed by a.out.800 t. 900 ms. a.a.yses of t.e spect.a. power densit.es of phot.n a.plit.de va.ia.ions from t.e left.a.d right.hemispheres revea.ed pea. va.ues bet.een 2 a.d 3 Hz which were equiva.ent.t. a.difference of a.out.900 ms. t.e ra.ia.t.flux densit.es were est.ma.ed t. be in t.e order of 10?12 W?m?2 a.d t. include t.e equiva.ence of a.out.107 neurons. Our ca.cula.ions, which a.count.d for t.e sma.l ma.nit.de of t.e st.engt. of t.e int.rhemispheric coefficient., suggest.t.a. t.e coherence could be st.ongly correla.ed wit. processes a.socia.ed wit. t.e unmyelina.ed a.ons wit. dia.et.rs bet.een 400 t. 800 nm, t.e visible wa.elengt.s, wit.in t.e corpus ca.losum. When t.e ra.io of t.e pha.e shift.wa. a.plied t. t.e a.a.a.ov-Bohm equa.ion, t.e t.me required for a.phot.n-rela.ed elect.on t. be wit.in a.cerebra. ma.net.c field wa. t.e sa.e dura.ion a. a.single orbit.of a. elect.on a.d a.phot.n’s t.a.ersa. la.ency a.ross a.neurona. pla.ma.membra.e. We suggest.t.a. t.e pea. phot.n decoherence bet.een t.e t.o cerebra. hemispheres ma. revea. a.neurona.-qua.t.0}subst.a.e t. t.e condit.ons a.socia.ed wit. consciousness.

关键词： Biophot.ns Int.rhemispheric Correla.ions Ima.ina.ion Huma. Bra.n Qua.t.t.0}ive EEG (QEEG) Visua.iza.ion Corpus Ca.losum a.a.a.ov-Bohm Effect

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