检索结果-南通市图书馆

Feline Hypertrophic Cardiomyopathy Associated with the p.A31P Mutation in cMyBP-C Is Caused by Production of Mutated cMyBP-C with Reduced Binding to Actin

引用

Open Journal of Veterinary Medicine 2013年第2期3卷 95-103页

作者： mia t.n.godiksen Craig Kinnear tina Ravnsborg Peter Hojrup Sara Granstrom Inga A.Laursen Paula L.Hedley Johanna C.Moolman-Smook William J.McKenna Jorgen Koch Michael Christiansen Department of Clinical Biochemistry and Immunology Statens Serum InstitutCopenhagenDenmark Department of Small Animals Diseases Faculty of Life ScienceUniversity of CopenhagenCopenhagenDenmark MRC Centre for Molecular and Cellular Biology University of StellenboschCape TownSouth Africa Institute of Biochemistry and Molecular Biology University of Southern DenmarkOdenseDenmark Institute of Cardiovascular Science The Heart HospitalUniversity College LondonLondonUK

Hypertrophic cardiomyopathy (HCM) is a myocardial disorder, with complications including heart failure, thromboemboli and sudden death. Human and feline HCM (fHCM) are clinically comparable, thus fHCM may serve as a spontaneous animal model. fHCM in Maine Coon (MC) cats is associated with the p.A31P mutation in the cMyBP-C protein. the mutation is located in the cMyBP-C C0-domain which is known to interact with actin. the presence and levels of the wild type and mutated protein in heart tissue from mutant and wild type MC cats were examined by SDS-PAGE and mass spectrometry (MS). Quantitative yeast-2-hybrid (Y2H) protein-protein interaction analysis was used to assess the effect of the mutation on C0C1/actin interaction. the nMR-based structure of the C0 domain was used to calculate the energetic consequence of replacing alanine with a proline residue. In the homozygous MC cat, the mutated cMyBP-C protein was present, and cMyBPC-C levels were not reduced compared to that of the wild type cat. However, the interaction of actin with mutant cMyBP-C C0C1 was reduced compared to that of wild type. this may be because the substitution of the alanine with proline in position 31 was energetically highly unfavorable and resulted in only one hydrogen bond within the anti-parallel beta-strand compared to two hydrogen-bonds for alanine, possibly destabilizing the structure of the actin-interacting domain. the p.A31P mutation is present in cardiac tissue and the most likely pathogenic mechanism is interference with contractility by reducing binding of the C0C1 domain of cMyBP-C to actin.

关键词： Feline HCM cMyBP-C Mutation Actin Y2H MSMS

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

nitrate reduction capacity of the oral microbiota is impaired in periodontitis:potential implications for systemic nitric oxide availability

引用

International Journal of Oral Science 2024年第1期16卷 52-61页

作者： Bob t.Rosier William Johnston Miguel Carda-Diéguez Annabel Simpson Elena Cabello-Yeves Krystyna Piela Robert Reilly Alejandro Artacho Chris Easton mia Burleigh Shauna Culshaw Alex Mira Department of Genomics and Health FISABIO FoundationCenter for Advanced Research in Public HealthValenciaSpain Department of Biological and Biomedical Sciences Glasgow Caledonian UniversityGlasgowUK Oral Sciences University of Glasgow Dental SchoolSchool of MedicineDentistry and NursingCollege of MedicalVeterinary and Life SciencesUniversity of GlasgowGlasgowUK Sport and Physical Activity Research Institute University of the West of ScotlandBlantyreScotland Instituto de Biomedicina de Valencia Consejo Superior de Investigaciones Científicas(IBV-CSIC)ValenciaSpain CIBER Center for Epidemiology and Public Health MadridSpain

the reduction of nitrate to nitrite by the oral microbiota has been proposed to be important for oral health and results in nitric oxide formation that can improve cardiometabolic conditions. Studies of bacterial composition in subgingival plaque suggest that nitrate-reducing bacteria are associated with periodontal health, but the impact of periodontitis on nitrate-reducing capacity(nRC)and, therefore, nitric oxide availability has not been evaluated. the current study aimed to evaluate how periodontitis affects the nRC of the oral microbiota. First, 16S rRnA sequencing data from five different countries were analyzed, revealing that nitratereducing bacteria were significantly lower in subgingival plaque of periodontitis patients compared with healthy individuals(P n all five datasets with n = 20–82 samples per dataset). Secondly, subgingival plaque, saliva, and plasma samples were obtained from 42 periodontitis patients before and after periodontal treatment. the oral nRC was determined in vitro by incubating saliva with 8 mmol/L nitrate(a concentration found in saliva after nitrate-rich vegetable intake) and compared with the nRC of 15healthy individuals. Salivary nRC was found to be diminished in periodontal patients before treatment(P t recovered to healthy levels 90 days post-treatment. Additionally, the subgingival levels of nitrate-reducing bacteria increased after treatment and correlated negatively with periodontitis-associated bacteria(P no significant effect of periodontal treatment on the baseline saliva and plasma nitrate and nitrite levels was found, indicating that differences in the nRC may only be revealed after nitrate intake. Our results suggest that an impaired nRC in periodontitis could limit dietary nitrate-derived nitric oxide levels, and the effect on systemic health should be explored in future studies.

关键词： impaired intake nitrate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Diagnosis of focal liver lesions with deep learning-based multichannel analysis of hepatocyte-specific contrast-enhanced magnetic resonance imaging

引用

World Journal of Gastroenterology 2021年第35期27卷 5978-5988页

作者： Róbert Stollmayer Bettina K Budai Ambrus tóth IldikóKalina Erika Hartmann Péter Szoldán Viktor Bérczi Pál Maurovich-Horvat Pál n Kaposi Department of Radiology Medical Imaging CentreFaculty of MedicineSemmelweis UniversityBudapest 1083Hungary Department of transplantation and Surgery Faculty of MedicineSemmelweis UniversityBudapest 1082Hungary MedInnoScan Research and Development Ltd. Budapest 1112Hungary

BACKGROUnD the nature of input data is an essential factor when training neural *** concerning magnetic resonance imaging(MRI)-based diagnosis of liver tumors using deep learning has been rapidly ***,evidence to support the utilization of multi-dimensional and multi-parametric image data is *** to higher information content,three-dimensional input should presumably result in higher classification ***,the differentiation between focal liver lesions(FLLs)can only be plausible with simultaneous analysis of multisequence MRI *** to compare diagnostic efficiency of two-dimensional(2D)and three-dimensional(3D)-densely connected convolutional neural networks(Densenet)for FLLs on multi-sequence *** We retrospectively collected t2-weighted,gadoxetate disodium-enhanced arterial phase,portal venous phase,and hepatobiliary phase MRI scans from patients with focal nodular hyperplasia(FnH),hepatocellular carcinomas(HCC)or liver metastases(MEt).Our search identified 71 FnH,69 HCC and 76 *** volume registration,the same three most representative axial slices from all sequences were combined into four-channel images to train the 2D-Densenet264 *** bounding boxes were selected on all scans and stacked into 4D volumes to train the 3D-Densenet264 *** test set consisted of 10-10-10 *** performance of the models was compared using area under the receiver operating characteristic curve(AUROC),specificity,sensitivity,positive predictive values(PPV),negative predictive values(nPV),and f1 *** the average AUC value of the 2D model(0.98)was slightly higher than that of the 3D model(0.94).Mean PPV,sensitivity,nPV,specificity and f1 scores(0.94,0.93,0.97,0.97,and 0.93)of the 2D model were also superior to metrics of the 3D model(0.84,0.83,0.92,0.92,and 0.83).the classification metrics of FnH were 0.91,1.00,1.00,0.95,and 0.95 using the 2D and 0.90,0.90,0.95,0.95,and 0.90 using the 3D ***

关键词： Artificial intelligence Multi-parametric magnetic resonance imaging Hepatocyte-specific contrast Densely connected convolutional network Hepatocellular carcinoma Focal nodular hyperplasia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Self-assembling protein nanocarrier for selective delivery of cytotoxic polypeptides to CXCR4^(+) head and neck squamous cell carcinoma tumors

引用

Acta Pharmaceutica Sinica B 2022年第5期12卷 2578-2591页

作者： Elisa Rioja-Blanco Irene Arroyo-Solera Patriciaálamo Isolda Casanova Alberto Gallardo Ugutz Unzueta naroa Serna Laura Sánchez-García Miquel Quer Antonio Villaverde Esther Vázquez Ramon Mangues Lorena Alba-Castellón Xavier León Institut d'InvestigacióBiomèdica Sant Pau(IIB-Sant Pau) Barcelona 08041Spain Institut de Recerca contra la Leucèmia Josep Carreras Barcelona 08025Spain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid 28029Spain Department of Pathology Hospital de la Santa Creu i Sant PauBarcelona 08041Spain Institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra 08193Spain Departament de Genètica i de Microbiologia Universitat Autònoma de BarcelonaBellaterra 08193Spain Department of Otorhinolaryngology Hospital de la Santa Creu i Sant PauBarcelona 08041Spain

Loco-regional recurrences and distant metastases represent the main cause of head and neck squamous cell carcinoma(HnSCC) mortality. the overexpression of chemokine receptor 4(CXCR4) in HnSCC primary tumors associates with higher risk of developing loco-regional recurrences and distant metastases, thus making CXCR4 an ideal entry pathway for targeted drug delivery. In this context, our group has generated the self-assembling protein nanocarrier t22-GFP-H6, displaying multiple t22 peptidic ligands that specifically target CXCR4. this study aimed to validate t22-GFP-H6 as a suitable nanocarrier to selectively deliver cytotoxic agents to CXCR4^(+)tumors in a HnSCC model. Here we demonstrate that t22-GFP-H6 selectively internalizes in CXCR4^(+)HnSCC cells, achieving a high accumulation in CXCR4^(+)tumors in vivo, while showing negligible nanocarrier distribution in non-tumor bearing organs. Moreover, this t22-empowered nanocarrier can incorporate bacterial toxin domains to generate therapeutic nanotoxins that induce cell death in CXCR4-overexpressing tumors in the absence of histological alterations in normal organs. Altogether, these results show the potential use of this t22-empowered nanocarrier platform to incorporate polypeptidic domains of choice to selectively eliminate CXCR4^(+)cells in HnSCC. Remarkably, to our knowledge, this is the first study testing targeted proteinonly nanoparticles in this cancer type, which may represent a novel treatment approach for HnSCC patients.

关键词： targeted drug delivery Protein nanoparticles CXCR4 receptor HnSCC Cell targeting Recombinant proteins nanotoxins Cancer therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

复方苦参注射液联合体外高频热疗治疗晚期肝癌腹腔积液的疗效分析

引用

辽宁中医杂志 2019年第4期46卷 779-782页

作者：张海光苗亚敬李世龙张宝司玉广唐山市人民医院肝胆外科河北唐山063000 滦州市人民医院血液透析科河北滦州063700 唐山市人民医院检验科河北唐山063001 唐山市传染病院检验科河北唐山063020 承德市第六医院急诊科河北承德067000

目的:探讨复方苦参注射液腹腔内注射联合体外高频热疗治疗晚期肝癌腹腔积液的疗效以及对患者免疫功能、生命质量及不良反应的影响。方法:将2012年3月—2017年2月唐山市人民医院肿瘤内科收治的102例晚期肝癌腹腔积液患者采用随机数字表... 详细信息

目的:探讨复方苦参注射液腹腔内注射联合体外高频热疗治疗晚期肝癌腹腔积液的疗效以及对患者免疫功能、生命质量及不良反应的影响。方法:将2012年3月—2017年2月唐山市人民医院肿瘤内科收治的102例晚期肝癌腹腔积液患者采用随机数字表法分为研究组(51例)和对照组(51例),对照组患者采用复方苦参注射液治疗,研究组在对照组基础上加用体外高频热疗。比较两组疗效,分析治疗前后免疫功能指标自然杀伤(nK)细胞、CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+的变化、观察患者生命质量改善及不良反应发生情况。结果:研究组CR 13例,PR 29例,有效率82. 35%,对照组CR 6例,PR 18例,有效率47. 06%,研究组有效率高于对照组(χ~2=13. 909,P=0. 000)。治疗后研究组nK细胞[(19. 72±1. 16:17. 21±1. 05),t=11. 456,P t=2. 190,P t=2. 901,P t=5. 040,P t=7. 288,P <0. 001]。研究组患者生命质量改善率高于对照组(82. 35%:52. 94%,χ~2=10. 079,P=0. 001),研究组不良反应发生率为5. 88%,对照组不良反应发生率为3. 92%,两组比较差异无统计学意义(χ~2=0. 210,P=0. 647)。结论:复方苦参注射液腹腔内注射联合体外高频热疗可提高晚期肝癌的治疗效果,提高患者免疫功能和生命质量,且不增加不良反应。

关键词：肝肿瘤腹水免疫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

可注射磷酸钙骨水泥填充固定羊胫骨平台骨折的体内生物力学研究

引用

中华创伤骨科杂志 2008年第9期10卷 862-866页

作者：苗军夏群刘春蓉张继东徐宝山白剑强黄鸿超天津医院脊柱外科 300211

目的比较磷酸钙骨水泥（CPC）与松质骨移植在修复羊外侧胫骨平台压缩性骨折中的体内生物力学特点。方法成年健康山羊10只，双侧后肢制成外侧胫骨平台压缩性骨折模型，一侧以CPC填充（CVC组），对侧用自体骨填充（自体骨组）。术后3个月... 详细信息

目的比较磷酸钙骨水泥（CPC）与松质骨移植在修复羊外侧胫骨平台压缩性骨折中的体内生物力学特点。方法成年健康山羊10只，双侧后肢制成外侧胫骨平台压缩性骨折模型，一侧以CPC填充（CVC组），对侧用自体骨填充（自体骨组）。术后3个月和6个月各处死5只动物，取双侧胫骨观察关节面塌陷程度，摄X线片观察骨水泥降解情况，测定骨折部位抗压刚度，制备不脱钙切片观察关节面组织学改变。结果自体骨组10个标本中的8个发生了骨折块的中重度下陷，CPE组只有3个发生了轻度下陷。X线片示3个月时骨水泥降解吸收被分割成几块，外形变模糊，6个月时大部分骨水泥被降解吸收。CPC组和白体骨组抗压刚度在3个月时分别为（226．89±62．81）n／mm和（172．97±79．82）n／mm，差异无统计学意义（t=1．369，P=0．243），6个月时分别为（471．274-212．89）n／mm和（371．05±207．98）n／mm，差异无统计学意义（t=0．948，P=0．397）。组织学观察显示自体骨组关节面塌陷较严重，修复的软骨组织中纤维组织偏多；CPC组关节面软骨修复过程中对位较好，由纤维软骨形成相连接，纤维组织较少。结论CPC的支撑效果好于自体骨，可在早期提供一定的抗压强度，防止胫骨平台关节面塌陷移位，有利于术后早期活动。

关键词：羊胫骨骨折生物力学磷酸钙骨水泥

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

微创尸检:替代传统尸检?

引用

国际医学放射学杂志 2009年第3期 284-284页

作者： A.C.Weustink M.G.Hunink C.F.van Dijke n.S.Renken G.P.Krestin J.W.Oosterhuis 周炳 Department of Radiology Erasmus University Medical Center RotterdamDr Molewaterplein 403000 CA Rotterdamthe Netherlands

目的确定检测死因的微创尸检(mia)的诊断表现,研究在临床上mia取代传统尸检(CA)的可行性。方法本方法和研究获机构审查委员会批准,并获得死者家属知情同意。30例死亡病人(男19例,女11例,年龄46～79岁),因医学目的,家属允许做传统尸检。... 详细信息

目的确定检测死因的微创尸检(mia)的诊断表现,研究在临床上mia取代传统尸检(CA)的可行性。方法本方法和研究获机构审查委员会批准,并获得死者家属知情同意。30例死亡病人(男19例,女11例,年龄46～79岁),因医学目的,家属允许做传统尸检。在接受传统尸检之前先接受另外的mia的评估。mia由全身16层Ct扫描和1.5t磁共振扫描完成,

关键词：尸检死因 mia 敏感度分析

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：