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3D打印微流控电泳芯片的电渗流性能研究

分析化学 2021年第11期49卷 1937-1944页

作者：刘辉刘萌萌杨元杰雷云刘爱林福建医科大学药学院药物分析系福建省高校纳米医药技术重点实验室福建医科大学附属第一医院药学部

为研究微通道电渗性能,利用两种制作工艺制备3D打印微流控电泳芯片:以光敏树脂为原料的立体光固化(SLA)型3D打印机制作了长度、截面积均相同,形状不同的4种微通道,包括正三角形、正方形、 3/4正二十边形和3/4圆形;以多聚合物线材为原料... 详细信息

为研究微通道电渗性能,利用两种制作工艺制备3D打印微流控电泳芯片:以光敏树脂为原料的立体光固化(SLA)型3D打印机制作了长度、截面积均相同,形状不同的4种微通道,包括正三角形、正方形、 3/4正二十边形和3/4圆形;以多聚合物线材为原料的熔融沉积成(FDM)型3D打印机制作了正方形的微通道。通过电流监测法,探究了缓冲溶液pH值、微通道形状、打印机类型及其制作方式对微流控电泳芯片电渗流性能的影响。结果表明,对于SLA型电泳芯片,其表面带正电荷,微通道电渗淌度与缓冲溶液的pH值呈负相关;4种不同形状微通道的电泳芯片中,正方形通道电渗淌度值最大;对于FDM型电泳芯片,其表面带负电荷,微通道电渗淌度随缓冲溶液的pH值增大而升高,并且曲线线性更佳,电渗淌度值更低。SLA型3D打印具有制作多样化微通道的性能,但制作过程中残留在微通道的树脂难以清除,导致微通道的制作稳定性受到影响;FDM型3D打印制作微通道稳定性较好,但微通道构型多样化方面受限。对基于3D打印技术的微流控电泳芯片微通道制作方法进行改进,并对微通道电渗性能进行探究,可为微流控电泳芯片制作及用于电泳分析提供理论和实验基础。

关键词：微流控电泳芯片 3D打印微通道电渗流

Rheb promotes brown fat thermogenesis by Notch-dependent activation of the PKA signaling pathway

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Journal of Molecular Cell Biology 2019年第9期11卷 781-790页

作者： Wen meng Xiuci Liang Ting Xiao Jing Wang jie Wen Hairong Luo Jianhui Teng Yanquan Fei Qinghai Zhang Bilian Liu Fang Hu juli Bai Meilian Liu Zhiguang Zhou Feng Liu Department of Metabolism and Endocrinology Second Xiangya HospitalCentral South UniversityChangsha 410011China Metabolic Syndrome Research Center Key Laboratory of Diabetes ImmunologyMinistry of EducationNational Clinical Research Center for Metabolic DiseasesSecond Xiangya HospitalCentral South UniversityChangsha 410011China Department of Pharmacology University of Texas Health Science Center at San AntonioSan AntonioTXUSA Department of Biochemistry and Molecular Biology University of New Mexico Health Sciences CenterAlbuquerqueNMUSA

Increasing brown and beige fat thermogenesis have an anti-obesity effect and thus great metabolic ***,the molecular mechanisms regulating brown and beige fat thermogenesis remain to be further *** recently found that fat-specific knockout of Rheb promoted beige fat *** the current study,we show that Rheb has distinct effects on thermogenic gene expression in brown and beige ***-specific knockout of Rheb decreased protein kinase A(PKA)activity and thermogenic gene expression in brown adipose tissue of high-fat diet-fed *** the other hand,overexpression of Rheb activated PKA and increased uncoupling protein 1 expression in brown ***,Rheb overexpression in brown adipocytes increased Notch expression,leading to disassociation of the regulatory subunit from the catalytic subunit of PKA and subsequent PKA *** study demonstrates that Rheb,by selectively modulating thermogenic gene expression in brown and beige adipose tissues,plays an important role in regulating energy homeostasis.

关键词： obesity thermogenesis beiging adipose tissue energy homeostasis

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论美国智库的功能和运行机制

外语研究 2019年第6期36卷 52-57页

作者：孟磊国防科技大学国际关系学院陆军工程大学外训大队

美国智库以其数量众多、影响力巨大闻名于世。本文呈现了美国智库现状及特点,从探究美国智库功能的思想和政治基础出发,分析了美国智库的产出政策思想、引导公众舆论、汇聚人才等三大功能,并针对智库的组织结构、资金来源、人才管理及... 详细信息

美国智库以其数量众多、影响力巨大闻名于世。本文呈现了美国智库现状及特点,从探究美国智库功能的思想和政治基础出发,分析了美国智库的产出政策思想、引导公众舆论、汇聚人才等三大功能,并针对智库的组织结构、资金来源、人才管理及研究产品等主要运行机制结合案例进行了分析讨论。

关键词：智库智库现状智库功能智库运行机制

Protein O-GlcNAcylation homeostasis regulates facultative heterochromatin to fine-tune sog-Dpp signaling during Drosophila early embryogenesis

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Journal of Genetics and Genomics 2023年第12期50卷 948-959页

作者： Yaowen Zhang Haibin Yu Dandan Wang Xiaoyun lei Yang meng Na Zhang Fang Chen Lu Lv Qian Pan Hongtao Qin Zhuohua Zhang Daan M.F.van Aalten Kai Yuan Center for Medical Genetics School of Life Sciences&Hunan Key Laboratory of Molecular Precision MedicineDepartment of OncologyXiangya HospitalCentral South UniversityChangshaHunan 410008China State Key Laboratory of Chemo/Biosensing and Chemometrics College of BiologyHunan UniversityChangshaHunan 410082China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Division of Cell and Developmental Biology School of Life SciencesUniversity of DundeeDundee DD15EHUK Department of Molecular Biology and Genetics University of AarhusAarhus 8000Denmark The Biobank of Xiangya Hospital Central South UniversityChangshaHunan 410008China

Protein O-GlcNAcylation is a monosaccharide post-translational modification maintained by two evolutionarily conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Mutations in human OGT have recently been associated with neurodevelopmental disorders, although the mechanisms linking O-GlcNAc homeostasis to neurodevelopment are not understood. Here, we investigate the effects of perturbing protein O-GlcNAcylation using transgenic Drosophila lines that overexpress a highly active OGA. We reveal that temporal reduction of protein O-GlcNAcylation in early embryos leads to reduced brain size and olfactory learning in adult Drosophila. Downregulation of O-GlcNAcylation induced by the exogenous OGA activity promotes nuclear foci formation of Polycomb-group protein Polyhomeotic and the accumulation of excess K27 trimethylation of histone H3 (H3K27me3) at the mid-blastula transition. These changes interfere with the zygotic expression of several neurodevelopmental genes, particularly short gastrulation (sog), a component of an evolutionarily conserved sog-Decapentaplegic (Dpp) signaling system required for neuroectoderm specification. Our findings highlight the importance of early embryonic O-GlcNAcylation homeostasis for the fidelity of facultative heterochromatin redeployment and initial cell fate commitment of neuronal lineages, suggesting a possible mechanism underpinning OGT-associated intellectual disability.

关键词： Protein O-GlcNAcylation Drosophila Early embryogenesis Polycomb repressive complex Facultative heterochromatin Neurodevelopment sog

Status of aquatic organisms resources and their environments in Yangtze River system(2017-2021)⋆

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Aquaculture and Fisheries 2024年第5期9卷 833-850页

作者： Haile Yang Li Shen Yongfeng He Huiwu Tian lei Gao Jinming Wu Zhigang Mei Nian Wei Lin Wang Tingbing Zhu Feifei Hu Jinling Gong Hongchun Du Xinbin Duan Huatang Deng Daoqun Wang Fengyue Zhu Yunfeng Li Fan Wu Huijun Ru Yan Zhang Junyi Li Junlin Yang Yuntao Zhou Dongdong Fang Yinping Wang Danqing Lin Yanping Yang Peijie Li Silei Liu Jian Yang Ping Zhuang Sikai Wang Tao Zhang Gang Yang Wenbo Yang Lilai Yuan Kun Cao Shuo Xu Huiyuan Liu Zhiqiang Liang Chongrui Wang Hong Li Xiping Yuan Xin Yang Yilong Fu Yanping Zhang Haixin Zhang Zhiying Tao Sheng Wang Xiaoping Gao Binsong Jin Kemao Li Guojie Wang Shenglong Jian Yingqin Li Chenjiang Xue Chunyun lei Shaowei Xue Yi Sun Bin Zhu Ke Shao Xingkun Hu Meihua Xiong Jun Du Bin He Tao Yan Yingying Huang Yuanchao Zou Biwen Xie Yongming Wang Bin Li Fei Liu Yaoyao Zhang Fei Fan Zhijian Wang Jing Huang Haoran Gu Hailong Ge Yan Dan Yan Li Shuqiao Wang Chuang Zhang Lu Zhou Xue Wang Sheng Zeng Yan Xiang Xugang He Jianhui Qin Chengxing Xia jie Hou Yifu Shi Lifang Gao Zhiqiang Zhu Hongbao Shen Yun Du Xuejun Duan Jiawu Xiong Deguo Yang Shaoping Liu Zhaohui Ni Hui Zhang Kai Liu Feng Zhao Yingren Li Jianwei Wang Qiwei Wei Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhan430223China Institute of Hydrobiology Chinese Academy of SciencesWuhan430072China Fishery Resource and Environment Research Center Chinese Academy of Fishery SciencesBeijing100141China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai200090China Institute of Fisheries Engineering Chinese Academy of Fishery SciencesBeijing100141China Hunan Fisheries Science Institute Changsha410153China Jiangxi Fisheries Research Institute Nanchang330039China Jiujiang Academy of Fishery Sciences Jiujiang332000China School of Life Sciences Nanchang UniversityNanchang330031China Qinghai Provincial Fishery Technology Extension Center Xining810012China Yunnan Academy of Fishery Sciences Kunming650111China Institute of Hydroecology Ministry of Water Resources and Chinese Academy of SciencesWuhan430079China Fisheries Institute Sichuan Academy of Agricultural SciencesChengdu611731China School of Life Sciences Neijiang Normal UniversityNeijiang641100China School of Life Sciences Southwest UniversityChongqing400715China Chongqing Fishery Sciences Research Institute Chongqing401120China Guizhou Fisheries Research Institute Guiyang550025China College of Fisheries Huazhong Agricultural UniversityWuhan430072China Hubei Fisheries Science Research Institute Wuhan430070China Shaanxi Fisheries Research Institute Xi’an710086China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430077China Nanjing Institute of Geography and Limnology Chinese Academy of SciencesNanjing210008China Central South Inventory and Planning Institute of State Forestry and Grassland Administration Changsha410014China

The Yangtze River is the mother river of *** promote the aquatic ecosystem protection of the great river,the Project of Yangtze Fisheries Resources and Environment Investigation(2017-2021)supported by the Ministry of Agriculture and Rural Affairs,*** carried out by 24 institutes and universities that located in the Yangtze River basin surveys the status of(1)fish species composition and spatial distribution,(2)current fish abundance,(3)endangered fishes,(4)Yangtze finless porpoise,(5)aquatic eco-environments,(6)water-level fluctuation areas,(7)capture fisheries and recreational fisheries of the Yangtze River mainstream and 10 of its main tributaries,including Yalong River,Heng River,Min River(including Dadu River),Chishui River,Tuo River,Jialing River,Wu River,Han River,Dongting Lake and Poyang *** results showed that there were 443 fish species(belonging to 163 genus,37 families,and 18 orders)before 2017,but only 323 fish species(including 15 new recorded exotic species)were recorded in the project of *** them,Cyprinus carpio,Carassius auratus,Hypophthalmichthys molitrix,Pelteobagrus fulvidraco,Coilia brachygnathus,Silurus asotus,Saurogobio dabryi,Ctenopharyngodon idellus,Pelteobagrus nitidus,Hemiculter leucisculus,Siniperca chuatsi,Coreius heterodon,Culter alburnus,Parabramis pekinensis,and Aristichthys nobilis were the dominant species across the whole Yangtze River *** is estimated that there were 886 million individuals weighing 124.8 million kg,merely equivalent to 27.3%of the resources in 1950s,30.9%of the resources in 1960s,or 58.7%of the resources in *** the new list of protected fishes that recorded in the Yangtze River system,only 15 of 29 were collected in this project of *** gladius has been affirmed to be Extinct by International Union for Conservation of Nature(IUCN).The wild individuals of Tenualosa reevesii and Luciobrama macrocephalus have disappeared for many years and maybe have been extinct

关键词： Fishes Yangtze finless porpoise Resources Aquatic ecosystem Fisheries Background investigation Yangtze river system

水氮耦合对渭北温室春茬番茄生长及土壤硝态氮累积的影响

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中国农学通报 2024年第22期40卷 103-109页

作者：孟延杨海燕张飞雷晓莹赵银平孙利萍刘滔赵增寿渭南市农业科学研究院陕西渭南714000 陕西省现代农业培训中心西安710000 蒲城县农业综合执法大队陕西蒲城715500

为探究陕西渭北地区早春茬设施番茄的适宜施氮量和灌水量,选用‘金棚10号’番茄,以当地设施番茄全生育期常规氮用量450 kg·N/hm^(2)+水用量3000 m^(3)/hm^(2)(C_(1)T_(1))为对照,设置不同的氮肥用量(C_(1)常规、C_(2)减氮20%、C3减氮4... 详细信息

为探究陕西渭北地区早春茬设施番茄的适宜施氮量和灌水量,选用‘金棚10号’番茄,以当地设施番茄全生育期常规氮用量450 kg·N/hm^(2)+水用量3000 m^(3)/hm^(2)(C_(1)T_(1))为对照,设置不同的氮肥用量(C_(1)常规、C_(2)减氮20%、C3减氮40%)和灌水梯度(T_(1)常规、T_(2)减水30%)共组成6个处理,通过水肥一体化方式在番茄不同生长期施肥,对比分析番茄产量、品质以及土壤硝态氮的变化。结果表明,所有减氮减水处理和常规处理之间番茄总产量均无显著性差异,但以施氮量为主因素时C3水平的平均产量显著低于常规和C_(2);除蛋白质含量外其余番茄品质指标均未表现出明显差异,隶属函数法显示C_(2)T_(2)处理的番茄品质结果综合排序最优;土壤硝态氮在地下0~200 cm累积量随施氮量的减少而减少,随灌水量的减少而增加,不同处理硝态氮累积量范围在1028.38~1302.80 kg/hm^(2),100 cm以上土层的累积量占0~200 cm总累积量的59.9%~69.5%。从水肥投入与产出效益的平衡来看,C_(1)T_(2)、C_(2)T_(1)与常规处理基本持平,C_(2)T_(2)较常规处理增加效益2050元/hm^(2)。

关键词：日光温室番茄减氮节水土壤硝态氮

Development of SNP markers using RNA-seq technology and tetraprimer ARMS-PCR in sweetpotato

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Journal of Integrative Agriculture 2017年第2期16卷 464-470页

作者： KOU meng XU Jia-lei LI Qiang LIU Ya-ju WANG Xin TANG Wei YAN Hui ZHANG Yun-gang MA Dai-fu Xuzhou Institute of Agricultural Sciences/Sweetpotato Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of SweetpotatoMinistry of Agriculture School of Life Science Jiangsu Normal University

The information of single nucleotide polymorphisms （SNPs） is quite unknown in sweetpotato. In this study, two sweetpotato varieties （Xushu 18 and Xu 781） were sequenced by Illumina technology, as well as de novo transcriptome assembly, functional annotation, and in silico discovery of potential SNP molecular markers. Tetra-primer Amplification Refractory Mutation System PCR （ARMS-PCR） is a simple and sufficient method for detecting different alleles in SNP locus. Total 153 sets of ARMS-PCR primers were designed to validate the putative SNPs from sequences. PCR products from 103 sets of primers were different between Xu 781 and Xushu 18 via agarose gel electrophoresis, and the detection rate was 67.32%. We obtained the expected results from 32 sets of primers between the two genotypes. Furthermore, we ascertained the optimal annealing temperature of 32 sets of primers. These SNPs might be used in genotyping, QTL mapping, or marker-assisted trait selection further in sweetpotato. To our knowledge, this work was the first study to develop SNP markers in sweetpotato by using tetra-primer ARMS-PCR technique. This method was a simple, rapid, and useful techn!que to develop SNP markers, and will provide a potential and preliminary application in discriminating cultivars in sweetpotato.

关键词： sweetpotato SNPs RNA-seq tetra-primer ARMS-PCR

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互联网使用与青少年自我认同的生态关系

心理科学进展 2005年第2期13卷 169-177页

作者：雷雳陈猛首都师范大学北京100037

互联网已经渗透到人们日常生活的方方面面,给社会及个人带来了广泛而深远的影响,正处于人生的关键时期的青少年更是首当其冲。依据Bronfenbrenner的生态学模型,该文从微系统、中系统、宏系统以及处于生态系统中心的青少年自身4个方面,... 详细信息

互联网已经渗透到人们日常生活的方方面面,给社会及个人带来了广泛而深远的影响,正处于人生的关键时期的青少年更是首当其冲。依据Bronfenbrenner的生态学模型,该文从微系统、中系统、宏系统以及处于生态系统中心的青少年自身4个方面,分别论述了互联网使用与青少年自我认同的形成与发展的密切关系,从而提供了有关互联网使用对青少年身心发展的影响方面的一些初步知识。

关键词：互联网使用人际关系同伴关系自我认同

基于三维CT图像的电弧增材制造高强AlCuMnZr合金的气孔缺陷及其控制研究

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金属加工（热加工） 2024年第10期 82-88页

作者：康士豪孟杰杨代婷马欣凯魏浩鹏解威豪敖豪杰周颖惠西安工业大学材料与化工学院陕西西安710021

研究了电弧增材制造(Wire and Arc Additive Manufacture,WAAM)高强AlCuMnZr合金的气孔缺陷形成机理及其控制方法。基于三维CT图像分析技术,对采用自然空冷(AC)、基板强制水冷(WC)及热等静压(HIP)后处理的薄壁件气孔缺陷分析。结果表明:... 详细信息

研究了电弧增材制造(Wire and Arc Additive Manufacture,WAAM)高强AlCuMnZr合金的气孔缺陷形成机理及其控制方法。基于三维CT图像分析技术,对采用自然空冷(AC)、基板强制水冷(WC)及热等静压(HIP)后处理的薄壁件气孔缺陷分析。结果表明:AC/WC/HIP试样的气孔率分别为1.29%、1.21%、0.45%。HIP处理可有效降低气孔率并提升合金力学性能。基板自然空冷下WAAM成形AlCuMnZr合金经HIP处理后,抗拉强度、屈服强度和伸长率分别为(270±18)MPa、(150±8)MPa和(9±1)%。

关键词：电弧增材制造 AlCuMnZr铝合金气孔缺陷热等静压 CT技术