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Application of Molecular Markers Linking to Cytoplasmic Male Sterile Loci to Assist Maintainer Line Selection and Their Selection Efficiency in Welsh Onion (Allium fistulosum L.)

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Agricultural Sciences in China 2010年第11期9卷 1571-1576页

作者： GAI Shu-peng meng xiang-dong College of Life Science Qingdao Agricultural University Qingdao 266109 P.R.China College of Horticulture Shandong Agricultural University Tai ＇an 271018 P.R. China

Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the breeding of cytoplasmic male sterility in welsh onion has a little progress due to the limitation of its biological characteristic and traditional selection approach. To study the feasibility and the efficiency of utilizing marker assisted selection for male sterile lines in welsh onion, one SCAR marker, SCS13, and one RAPD marker, S2002400, which could distinguish between N and S cytoplasm in several welsh onion cultivars, were identified. The two markers were then confirmed by Southern blotting, and used to screen the N or S cytoplasm of individual plants in seven welsh onion cultivars in this study. Male sterile and fertile plants were evaluated by aceto-carmine dying. The frequency of N-cytoplasmic plants and maintainer genotype was calculated in the seven open populations of welsh onion. The minimum number of plants needed to identify a maintainer was evaluated to be 95% reliable. Results showed that 20 to 80% decrease of crosses and self-crosses for identifying a maintainer genotype could be achieved by the marker-assisted selection compared with traditional selection method. It was proved that the molecular markers could precisely identify cytoplasmic types individually, performed by one generation of cross and two generations of testcrosses and self-crosses. Finally, several maintainer genotype plants were selected with the help of the two markers in the seven cultivars. The screened markers could assist and accelerate sterile and maintainer lines selection with less labor and cost.

关键词： Alliumfistulosum L. CMS (cytoplasmic male sterility) markers-assisted selection molecular marker efficiency

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不同灌溉模式对胡杨根蘖苗功能性状的影响

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节水灌溉 2025年第2期 100-106页

作者：孟祥东凌红波张广朋张凌凯王子康覃小林新疆农业大学水利与土木工程学院新疆乌鲁木齐830052 新疆水利工程安全与水灾害防治重点实验室新疆乌鲁木齐830052 中国科学院新疆生态与地理研究所新疆乌鲁木齐830011 中国科学院大学北京100049

为巩固塔里木河下游输水成效,需探明最适宜胡杨根蘖苗生长的灌溉模式,以促进区域生态恢复。根蘖苗作为胡杨种群的主要增殖方式和生态恢复的先锋物种,其功能性状是反映生存策略的重要指标,但相关研究仍较为有限。因此,研究在昆阿斯特生... 详细信息

为巩固塔里木河下游输水成效,需探明最适宜胡杨根蘖苗生长的灌溉模式,以促进区域生态恢复。根蘖苗作为胡杨种群的主要增殖方式和生态恢复的先锋物种,其功能性状是反映生存策略的重要指标,但相关研究仍较为有限。因此,研究在昆阿斯特生态闸口附近,进行了不同灌溉模式对胡杨根蘖苗功能性状影响的野外试验。通过设置3种不同的灌溉梯度,研究灌溉频次、灌溉水量和灌溉持续时间对土壤理化性质及胡杨根蘖苗叶片功能性状的影响。研究结果表明:在F2灌溉模式下,土壤的全氮、全磷含量以及根蘖苗的叶面积(LA)、干物质含量(LDMC)和氮磷比(LN/LP)均显著高于F1和F3灌溉模式。相比之下,F1灌溉模式导致土壤全盐含量较高,进而引起根蘖苗LDMC和LN/LP的下降,同时比叶面积(SLA)有所上升。进一步研究表明,土壤全氮对叶面积的正向影响最为显著,贡献率达到52.4%。相反,土壤全盐含量对LDMC和LN/LP产生了抑制作用,但对SLA具有促进作用,贡献率为31.6%。土壤全磷、pH和含水率对根蘖苗功能性状的总体贡献率较低,仅为16%。因此,根据不同灌溉模式下根蘖苗长势及土壤理化性质对根蘖苗功能性状的影响,确定出F2灌溉模式(灌溉频次23 d,每10 m断根沟每次灌溉水量6 m^(3),灌溉持续时间2 d)更有利于土壤养分积累,进而有效的促进胡杨根蘖苗的生长。这一研究结果为塔里木河下游胡杨林的生态恢复提供了有力的参考。

关键词：塔里木河下游灌溉模式根蘖苗功能性状理化性质

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Decentralized fault diagnosis of discrete-event systems with unreliable sensors using linear temporal logic

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Science China(Information Sciences) 2025年第1期68卷 322-341页

作者： Weijie dong Shaoyuan LI xiang YIN Key Laboratory of System Control and Information Processing Ministry of EducationDepartment of Automation Shanghai Jiao Tong University

In this paper,we investigate a decentralized diagnosis problem of a discrete-evnt system(DES) subject to unreliable sensors,where the sensor observations of local diagnosers may be non-deterministic as a result of possible *** studies on decentralized robust diagnosis can only deal with different types of sensor failures separately,e.g.,all sensors suffer from the same type of sensor failures such as intermittent sensor failures or permanent sensor ***,since sensors of different local diagnosers may face different external environments and have different internal characteristics,sensors corresponding to different local diagnosers may also have their own fault *** this paper,we propose a flexible framework of decentralized diagnosis for DES subject to unreliable sensors such that sensors of different local diagnosers are permitted to have different types of sensor *** this end,we extend the existing decentralized diagnosis framework to the case where there exist common sensors broadcasting their observations to all local *** apply linear temporal logic(LTL) to constrain infinite behaviors of private sensors of local diagnosers and common ***,a new notion of φ-codiagnosability is proposed as the necessary and sufficient condition for the existence of a decentralized diagnoser that works correctly under sensors,satisfying LTL-based sensor ***,we provide an effective approach to verify the φ-codiagnosability.

关键词： discrete event systems decentralized diagnosis codiagnosability automata

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The regulatory network and critical factors promoting programmed cell death during embryogenesis

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Journal of Integrative Plant Biology 2025年第1期67卷 55-70页

作者： An Luo Ce Shi Pan Luo Zifu Zhao meng-xiang Sun College of Life Sciences Yangtze UniversityJingzhou 434023China State Key Laboratory of Hybrid Rice College of Life SciencesWuhan UniversityWuhan 430072China

Programmed cell death(PCD)is essential for animal and plant ***,the knowledge of the mechanism regulating PCD in plants remains limited,largely due to technical ***,we determined that the protease NtCP14 could trigger PCD in the embryonic suspensor of tobacco(Nicotiana tabacum),providing a unique opportunity to overcome the limitations by creating synchronous two-celled proembryos with ongoing PCD for transcriptome analysis and regulatory factor ***,we performed comparative transcriptome analysis using isolated two-celled proembryos and explored the potential regulatory network underlying NtCP14-triggered *** phytohormones,calcium,microtubule organization,the immunity system,soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins,long non-coding RNAs and alternative splicing are addressed as critical factors involved in the early stage of suspensor *** thought to play crucial roles in suspensor PCD are ***,decreased antioxidant gene expression and increased reactive oxygen species(ROS)levels during suspensor PCD suggest a critical role for ROS signaling in the initiation of NtCP14-triggered ***,five genes in the regulatory network are recommended as immediate downstream elements of ***,our analysis outlines an overall molecular network underlying protease-triggered PCD and provides a reliable database and valuable clues for targeting elements immediately downstream of NtCP14 to overcome technical bottlenecks and gain deep insight into the molecular mechanism regulating plant PCD.

关键词： programmed cell death protease reactive oxygen species suspensor transcriptome

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干涉合成孔径雷达的垂直基线对图像相干性的影响

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电子学报 2008年第6期36卷 1222-1226页

作者：孟祥东王彤保铮西安电子科技大学雷达信号处理国家重点实验室陕西西安710071

本文指出了InSAR中垂直基线是影响图像相干性的重要因素,论证了现有的直接计算相关系数的公式是基于理想信号得出的,然后结合实际雷达信号的频率特性推导出了一个直接计算相关系数的公式,并给出了在各种斜坡地形情况下相关系数与垂直基... 详细信息

本文指出了InSAR中垂直基线是影响图像相干性的重要因素,论证了现有的直接计算相关系数的公式是基于理想信号得出的,然后结合实际雷达信号的频率特性推导出了一个直接计算相关系数的公式,并给出了在各种斜坡地形情况下相关系数与垂直基线的变化关系.这种计算方法及其数值结果可用于对干涉合成孔径雷达的基线设计和性能评估.

关键词：干涉合成孔径雷达高程测量地面动目标检测垂直基线相干性

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溶胶-凝胶自燃烧制备CoY_(0.1)Fe_(1.9)O_4铁氧体的反应特性

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材料科学与工艺 2009年第6期17卷 858-862页

作者：孟祥东李海波吉林师范大学物理学院吉林四平136000 哈尔滨工业大学复合材料与结构研究所哈尔滨150001

研究了溶胶-凝胶自燃烧法制备CoY0.1Fe1.9O4的成胶热力学条件和自燃烧的过程,从理论和实验讨论了pH值和柠檬酸量对产物的影响.利用热重/差热综合分析仪及X射线衍射仪对干凝胶的热反应特性和晶体结构进行了测试和分析.利用振动样品磁强... 详细信息

研究了溶胶-凝胶自燃烧法制备CoY0.1Fe1.9O4的成胶热力学条件和自燃烧的过程,从理论和实验讨论了pH值和柠檬酸量对产物的影响.利用热重/差热综合分析仪及X射线衍射仪对干凝胶的热反应特性和晶体结构进行了测试和分析.利用振动样品磁强计研究了不同柠檬酸量产物的矫顽力.实验结果表明,由Fe、Co和Y元素与水的二元体系的平衡电势(E)-pH图确定的溶液成胶pH值与实验结果相符合;通过计算氧化剂正价态和燃料的负价态的平衡系数,获得混合硝酸盐与柠檬酸的理论比值为2.3.取过量的柠檬酸量,能够合成尖晶石结构的CoY0.1Fe1.9O4纳米粉末.随着柠檬酸量的继续增加,产物中有Fe2O3的出现,且矫顽力减小.

关键词：溶胶-凝胶铁氧体自燃烧矫顽力

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Y^(3+)掺杂对CoFe_(2-x)Y_xO_4铁氧体离子占位的影响

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人工晶体学报 2010年第3期39卷 780-783,787页

作者：孟祥东李海波吉林师范大学物理学院四平136000 哈尔滨工业大学复合材料与结构研究所哈尔滨150001

采用溶胶-凝胶自燃烧法制备了CoFe2-xYxO4(x=0,0.1,0.2,0.3,0.4,0.5)铁氧体纳米粉末体,进行了500℃热处理。采用VSM测试了其比饱和磁化强度,根据比饱和磁化强度(σs)与总磁化强度(Ms)的关系计算了Y3+掺杂对晶格中由B位迁移到A位的Co2+... 详细信息

采用溶胶-凝胶自燃烧法制备了CoFe2-xYxO4(x=0,0.1,0.2,0.3,0.4,0.5)铁氧体纳米粉末体,进行了500℃热处理。采用VSM测试了其比饱和磁化强度,根据比饱和磁化强度(σs)与总磁化强度(Ms)的关系计算了Y3+掺杂对晶格中由B位迁移到A位的Co2+物质的量的值(y);随着Y3+掺杂量的增加,迁移的Co2+的物质的量先增加后减少。并用Mssbauer谱技术进行了佐证,根据拟合的数据分析了Y3+掺杂后晶体中A位和B位的Fe3+的迁移规律;讨论了A位和B位的峰面积比值。

关键词：穆斯堡尔谱钴铁氧体掺杂离子占位

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Vacuum ammonia stripping from liquid digestate:Effects of pH,alkalinity,temperature,negative pressure and process optimization

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Journal of Environmental Sciences 2025年第3期149卷 638-650页

作者： Qiuhong Chen donghai Yang xiang Chen Xiankai Wang Bin dong Xiaohu Dai State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China YANGTZE Eco-Environment Engineering Research Center National Engineering Research Center of Eco-environment Protection for Yangtze River Economic BeltChina Three Gorges CorporationWuhan 430010China

High ammonia-nitrogen digestate has become a key bottleneck limiting the anaerobic digestion of organic solid *** ammonia stripping can simultaneously remove and recover ammonia nitrogen,which has attracted a lot of attention in recent *** investigate the parameter effects on the efficiency and mass transfer,five combination conditions(53℃ 15 kPa,60°C 20 kPa,65°C 25 kPa,72°C 35 kPa,and 81°C 50 kPa)were conducted for ammonia stripping of sludge *** results showed that 80%of ammonia nitrogen was stripped in 45 min for all experimental groups,but the ammonia transfer coefficient varied under different conditions,which increased with the rising of boiling point temperature,and reached the maximum value(39.0 mm/hr)at 81°C 50 *** ammonia nitrogen removal efficiency was more than 80%for 30 min vacuum stripping after adjusting the initial pH to above 9.5,and adjustment of the initial alkalinity also affects the pH value of liquid *** was found that pH and alkalinity are the key factors influencing the ammonia nitrogen dissociation and removal efficiency,while temperature and vacuum mainly affect the ammonia nitrogen mass transfer and removal *** terms of the mechanism of vacuum ammonia stripping,it underwent alkalinity destruction,pH enhancement,ammonia nitrogen dissociation,and free ammonia *** this study,two-stage experiments of alkalinity destruction and ammonia removal were also carried out,which showed that the two-stage configuration was beneficial for ammonia *** provides a theoretical basis and practical technology for the vacuum ammonia stripping from liquid digestate of organic solid waste.

关键词： Liquid digestate Vacuum ammonia stripping Alkalinity Mass transfer Process optimization

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Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)

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Rice science 2010年第3期17卷 173-178页

作者： CHEN Tao TIAN meng-xiang ZHANG Ya-dong ZHU Zhen ZHAO Ling ZHAO Qing-yong LIN Jing ZHOU Li-hui WANG Cai-lin Jiangsu High Quality Rice Research and Development Center Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014 China＂ #These authors contributed equally to this paper

Rice with low glutelin content is suitable as functional food for patients affected by kidney failure. Low glutelincontent gene Lgc1 in rice has a 3.5-kb deletion between two highly similar glutelin genes GluB4 and GluB5, which locates on the short arm of chromosome 2. To improve the selection efficiency in low glutelin-content rice breeding, two molecular markers designated as InDel-Lgc1-1 and InDel-Lgc1-2 were developed to detect the low glutelin-content gene Lgc1. A double PCR detection indicated that combined use of the two markers could easily distinguish the genotypes of Lgc1 from different rice varieties. Therefore, as a simple and low-cost technique, the molecular marker could be widely used to identify different varieties with Lgc1 gene and applied in marker-assisted selection of low glutelin-content rice.

关键词： Oryza sativa low glutelin-content gene functional marker double polymerase chain reaction

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High-resolution imaging through dynamic scattering media:Iterative optimization based on polarimetric characteristics

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Science China(Technological Sciences) 2025年第1期68卷 78-80页

作者： meng xiang Kai DENG Tianyu WANG Jinpeng LIU Xue dong Teli XI Fei LIU Xiaopeng SHAO School of Optoelectronic Engineering Xidian UniversityXi’an 710071China Xi’an Key Laboratory of Computational Imaging Xi’an 710071China National Key Laboratory of Infrared Detection Technologies Shanghai 200083China

High-resolution imaging through randomly dynamic scattered fields and highly scattered walls is an extensively sought-after capability with potential applications in various fields such as underwater imaging,biomedical imaging,and seeing through *** methods have been proposed to unscramble object information from degraded scattered images,resulting in considerable improvements in image contrast in degraded scenarios[1].

关键词： scattered dynamic resolution

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