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Calcitriol induces post-thawed bovine sperm capacitation

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BIOCELL 2023年第5期47卷 1135-1143页

作者： ANA CECILIA LIAUDAT FRANCISCA EBEL BIANCA ANA OPIZZO MARINA AURORA gonzalez DAMIÁN BLOIS PABLO BOSCH NANCY rodriguez Departamento de Biología Molecular Instituto de Biotecnología Ambiental y Salud(INBIAS)Río Cuarto5800Argentina

Background:Capacitation is a set of physiological changes sperms undergo to acquire fertilizing *** vivo,this process is directly associated with high calcium levels in sperm ***,the vitamin D hypercalcemic metabolite,is related to human sperm motility,capacitation,and acrosome *** work aimed to study the effect of calcitriol on bull sperm quality parameters and ***:One million freezethawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 *** cells(negative control)and treated ones with calcitriol or heparin(100μg/mL,positive capacitation control)were evaluated for motility,viability,and functional ***(70μM,30 min)treatment was included as a reactive oxygen species(ROS)positive sperm ***:The results elucidated that sperm exposed to 20 nM calcitriol showed higher viability,vigor,and capacitation than their positive and negative *** percentage of sperm with intact plasma and acrosome membranes,mitochondrial membrane potential(ΔΨm),and phosphatidylserine externalization was similar in all the conditions evaluated,while ROS production was higher with heparin and menadione-treated groups than the calcitriol group or negative ***:Our results indicate that calcitriol induces the capacitation of thawed bull spermatozoa and maintains acceptable values of progressive motility,viability,and vigor without altering key biological parameters such as redox status,ΔΨm,and cell death.

关键词： Bovine Sperm capacitation Calcitriol Cell viability Plasma membrane integrity Reactive oxygen species

Highly accessible aqueous synthesis of well-dispersed dendrimer type platinum nanoparticles and their catalytic applications

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Nano Research 2019年第5期12卷 1083-1092页

作者： Adrian Fernandez-Lodeiro Jamila Djafari David Lopez-Tejedor Carlos Perez-Rizquez Benito rodriguez-gonzalez Jose Luis Capelo Jose M.Palomo Carlos Lodeiro Javier Fernandez-Lodeiro BIOSCOPE Group LAQV@REQUIMTEChemistry DepartmentFaculty of Science and TechnologyUniversity NOVA of LisbonCaparica CampusCaparica 2829-516Portugal PROTEOMASS Scientific Society Rua dos InventoresMadan ParqueCaparica CampusCaparica 2829-516Portugal Department of Biocatalysis Institute of Catalysis(CSIC)MadridSpainMarie Curie 2CantoblancoUAM CampusMadrid 28049Spain Scientific and Technological Research Assistance Center(CACTI) University of VigoLagoas-MarcosendeVigo 36310Spain

The application of novel methodologies to the synthesis of nanomaterials is still a challenge in many different technological and scientific *** efficient and reproducible synthetic methodologies,that produce fewer residues and reduce the cost of raw materials must be *** the present work,we have explored the attractive possibility to apply the cheap iron (Ⅱ) sulphate salt in the reduction process of the K2PtCl4 to produce colloids *** synthesis took places in water and was assisted by sodium citrate (SC) using polyvinylpyrrolidone (PVP) as a *** adjustment of this novelty process allows obtaining well-dispersed and sub-20 nm dendrimer-type platinum nanoparticles (Pt D-NPs).The nano-dendrimers produced have been characterized using dynamic light scattering (DLS),transmission electron microscopy (TEM),high-resolution TEM (HR-TEM),XRD spectroscopy,inductive couple plasma (ICP) analysis,Fourier transform infrared (FT-IR) and ultraviolet-visible (UV-vis) *** conformational results derived from the size and shape will be *** application of the Pt D-NPs has been explored in the reduction of p-nitrophenol (p-NP) to p-aminophenol (p-AP) in aqueous media at room temperature obtained TOF value of 253 min^-***,our Pt D-NPs were tested as artificial metalloenzyme showing catechol oxidase activity for oxidation of L-DOPA.

关键词： platinum dendrimer NPs catalytic application green synthesis cateohol oxidase-like activity

The Human Rightsin Galician Press （FaroDe Vigo and La Voz De Galicia）

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Journalism and Mass Communication 2013年第11期3卷 718-727页

作者： Aurora Garcia gonzalez Beatriz Martinez rodriguez University ofVigo Vigo Spain

Democratic society requires that press freedom is reflected through the preservation of human rights. The aim of this study was to analyse quantitatively the media discourse in the environment of Galicia to see how they deal with different aspects related to the subject. The papers used for the study were for the months from January to September Faro fie Vigo and La Voz de Galicia, in order to establish whether there is a constant in the treatment is done, especially in regard to topics, dedicated spaces, sources and so on.

关键词： human rights press Galician

Wood Density of Ten Native Trees and Shrubs and Its Possible Relation with a Few Wood Chemical Compositions

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American Journal of Plant Sciences 2016年第8期7卷 1192-1197页

作者： Ratikanta Maiti Humberto gonzalez rodriguez Aruna Kumari Facultad de Ciencias Forestales Universidad Autónoma de Nuevo León Linares México Agricultural College Professor Jaya Shankar Telangana State Agricultural University Jagtial India

The present study was undertaken in Forest Science Faculty, Universidad de Nuevo Leon, Mexico on variability of Wood density and its possible relation to few wood chemical composition and wood fiber cell structure anatomy. The results reveal that among 10 specie studied, there exist a large variation in wood density (0.51 to 1.09), and few wood chemical composition such % carbón (37.14 to 44.07), nitrogen (9.18 to 19.22), sulphur (31.45 to 33/82), lignin (15/28 to 24.35), hemicellulose (19.94 to 27.36%), and % cellulose (33.69 to 45.92). In general, though there was no clear relationship between wood density and other chemical composition of wood. It was observed that the species having moderate to high wood density contained >40% carbón, >30% sulphur and >40% cellulose and more or less 20% lignin. It seems that carbón, sulphur, cellulose and lignin content contribute to greater density. The wood fiber cell with wall lignification seems to be related to higher wood density.

关键词： Wood Density Wood Chemical Composition Cellulose Lignin Carbón Concentration Variability

Democratic Transition in Mexico： Case of City Juarez

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International Relations and Diplomacy 2017年第3期5卷 134-152页

作者： Sergio Pacheco gonzalez Jesus Alberto rodriguez Alonso Universidad Autonoma de Ciudad Juarez Ciudad Juarez Mexico

The electronic and bibliographic sources, as well as some interviews and focus groups conducted in Ciudad Juarez, Chihuahua, all present interpretations by different actors on the so-called democratic transition in Mexico. The model, proposed Carothers （2002） to explain the transition from a dictatorship to democracy, as stage universally. This article shows which of the five assumptions of the model are present in the experience of the Mexican transition and those that were not consider applicable. He also argues that the model shows features of a teleological perspective, by omitting the particularities of each country, eliminating any reference to diversity and difference, becoming an ideological approach linked to the process of economic globalization. It criticizes the absence of discussion of social and economic objectives that should be priority in a conception of substantive democracy, rather than limiting the horizon of the transition to a liberal and normative conception, that assumes two basic formulas：（1） Democracy is equal to elections; and （2） a citizen is a vote. Contrasting documents on electoral disenchantment in Ciudad Juarez and the actions that various political actors have done, we can illustrate what can be termed substantive citizenship.

关键词： democratic transition authoritarianism electoral democracy liberalization political regime

Oral allopurinol to prevent hyperamylasemia and acute pancreatitis after endoscopic retrograde cholangiopancreatography

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World Journal of Gastroenterology 2009年第13期15卷 1600-1606页

作者： Hector Martinez-Torres Xochilt rodriguez-Lomeli Carlo Davalos-Cobian Jesus Garcia-Correa Juan Manue Maldonado-Martinez Fabiola Medrano-Muoz Clotilde Fuentes-Orozco Alejandr gonzalez-Ojeda Department of Gastroenterology an Endoscopy UMAE-Hospital de Especialidades del Centr Medico Nacional de Occidente-IMSS Research Unit in Clinical Epidemiology UMAE-Hospital de Especialidades del Centro Medico Naciona de Occidente-IMSS

AIM:To assess the efficacy of allopurinol to prevent hyperamylasemia and pancreatitis after endoscopic retrograde cholangiopancreatography(PEP).METHODS:One hundred and seventy patients were enrolled and randomized to two groups:a study group(n=85)who received 300 mg of oral allopurinol at 15 h and 3 h before endoscopic retrograde cholangiopancreatography(ERCP)and a control group(n=85)receiving an oral placebo at the same *** Outcome Measurements included serum amylase levels and the number severity of the episodes of *** amylase levels were classified as normal(151 IU/L).Episodes of PEP were classified following Ranson's criteria and CT severity ***:Gender distribution was similar between *** age was 53.5±18.9 years for study group and 52.8±19.8 years for ***,the distribution of benign pathology was similar between *** was more common in the control group(P=0.003).Mild PEP developed in two patients from the study group(2.3%)and eight(9.4%) from control group(P=0.04),seven episodes were observed in high-risk patients of the control group(25%) and one in the allopurinol group(3.3%,P=0.02).Risk factors for PEP were precut sphincterotomy(P=0.02),pancreatic duct manipulation(P=0.002)and multiple procedures(P=0.000).There were no deaths or side ***:Oral allopurinol before ERCP decreased the incidences of hyperamylasemia and pancreatitis in patients submitted to high-risk procedures.

关键词： Endoscopic retrograde cholangiopancreatography Hyperamylasemia Acute pancreatitis Oralallopurinol Risk factors

Seasonal variation in native hydraulic conductivity between two deciduous oak species

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Journal of Plant Ecology 2020年第1期13卷 78-86页

作者： Dilia Mota-Gutiérrez Guadalupe Arreola-González Rafael Aguilar-Romero Horacio Paz Jeannine Cavender-Bares Ken Oyama Antonio gonzalez-rodriguez Fernando Pineda-García Facultad de Biología Universidad Michoacana de San Nicolás de Hidalgo.Edificio RCiudad UniversitariaGral.Francisco J.Múgica S/N A-1Felicitas de Río58030 MoreliaMichoacánMexico Escuela Nacional de Estudios Superiores Unidad MoreliaUniversidad Nacional Autónoma de México.Antigua Carretera a Pátzcuaro 8701Ex-Hacienda de San Joséde La Huerta58190 MoreliaMichoacánMexico Instituto de Investigaciones en Ecosistemas y Sustentabilidad Campus MoreliaUniversidad Nacional Autónoma de México.Antigua Carretera a Pátzcuaro 8701Ex-Hacienda de San Joséde La Huerta58190 MoreliaMichoacánMexico Deparment of Ecology Evolution and BehaviorUniversity of MinnesotaSaint PaulMN 55108USA

Aims Mechanisms of plant drought resistance include both tolerance and *** vulnerability to embolism and turgor loss point are considered traits that confer tolerance,while leaf abscission and deciduousness characterizes the avoidance *** these mechanisms are thought to trade-off expressing a continuum among species,little is known on how variation in the timing and duration of leaf shedding in response to drought affect the relationship between xylem and leaf *** the present study,we explored the extent to which drought tolerance differs between two oak(Quercus)species that exhibit different leaf shedding ***,we predicted that *** Trel.,which loses leaves at the end of the dry season(late-deciduous)and is thus exposed to a greater risk of cavitation,would be more drought tolerant and more conservative in its water use than *** Liebm.,which loses its leaves for only a short period of time in the middle of the dry season(brevideciduous).Methods The study was conducted in central Mexico in a single population of each of the two oak species,separated from each other by a distance of 1.58 km,and by an altitudinal difference of 191 *** deserticola(late deciduous)is more frequent down slope,while ***(brevideciduous)tends to occur at higher elevations along the *** assessed seasonal differences(rainy versus dry season)in native stem hydraulic conductivity,and tested for variation in xylem vulnerability to cavitation,leaf water use and leaf turgor loss point between the two *** Findings The two oak species did not differ in traits conferring drought tolerance,including xylem vulnerability to embolism,leaf turgor loss point,or stomatal ***,both species had different performance during the dry season;the brevideciduous species had lower negative impact in the xylem function than the late-deciduous ***,seasonal changes in plant physiological performance

关键词： Quercus hydraulic conductivity embolisms leaf phenology

Short-term outcomes after the loading phase of intravitreal bevacizumab and subthreshold macular laser in non-center involved diabetic macular edema

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International Journal of Ophthalmology(English edition) 2018年第6期11卷 981-985页

作者： Edgar Cuervo-Lozano Jesus Hernan gonzalez-Cortes Abraham Olvera-Barrios Ezequiel Trevino-Cavazos Josue rodriguez-Pedraza Karim Mohamed-Noriega Jesus Mohamed-Hamsho Ophthalmology Department University Hospital "Jose Eleuterio Gonzalez" and Faculty of Medicine the Autonomous University of Nuevo Leen Monterrey 64460 Mexico

AIM： To compare the effectiveness of intravitreal bevacizumab and subthreshold macular photocoagulation （SMP）, for the treatment of non-center involved diabetic macular edema （non-CI DME）. METHODS： Prospective, randomized, controlled clinical study included patients with type 2 diabetes, non-CI DME and best-corrected visual acuity （BCVA） of 0.30 logMAR or better. Each eye was randomized into three groups： group 1, monthly intravitreal bevacizumab; group 2, single SMP; group 3, single SMP and monthly bevacizumab. Main outcome measures were BCVA, and macular thickness measured with optical coherence tomography as macular central subfield thickness （CST）, macular area of greater thickness （MAGT） and total macular volume （TMV）. Results were analyzed after 3mo. RESULTS： A total of 32 eyes were included. Group 3 improved in BCVA （0.19±0.16 to 0.12±0.14 logMAR; P=0.041） and in TMV （7.90±0.57 to 7.65±0.73 mm3; P=0.025）. Group 1 improved in MAGT （325±26.26 to 298.20±44.85 μm; P=0.022） and TMV （7.79±0.57 to 7.50±0.56 mm3, P=0.047）. Group 2 didn’t show significant improvement of any variable. CONCLUSION： The loading phase of bevacizumab as monotherapy or combined with SMP is superior to SMP as monotherapy in providing short-term visual and anatomical improvement in non-CI DME.

关键词： non-center involved diabetic macular edema intravitreal bevacizumab subthreshold macular photocoagulation intravitreal antiangiogenic

Determination of Fungal Colonization in the Oral Cavity of College Students

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Journal of Life Sciences 2012年第10期6卷 1133-1141页

作者： Floridia Ricardo rodriguez Graciela Ampuero Veronica gonzalez Luis Department of Parasitology and Mycology National University of San Luis San Luis 5700 Argentina

In recent years, an increase in opportunistic fungal colonization in the oral cavity in immunocompetent patients （IC） has been observed. In the bibliography, the most observable genre is Candida and less frequently found are other opportunistic such as Aspergillus, Rhizopus, Cryptococcus, and others. The authors determined the presence of fungi in the oral cavity of IC students, and their relationship with the concentration of secretory IgA in saliva. To this end, we collected 50 samples of oral cavity swabs, which underwent direct examination and culture in Sabouraud dextrose agar with chloramphenicol. For its identification, CHROMagar Candida and API Candida （BioMerieux） were used. We obtained nine positive cultures （7 Candida albicans and 2 Saccharomyses cerevisiae）, which represented 18% of the studied population. Throughout radial immunodiffusion （RID plates-PLATE）, we determined the concentration of secretory IgA. No relationship was observed between the colonized group and group that was not colinized. The colonization rate found is below the one described in the bibliography （30% to 50%）. However, these opportunistic fungi cause transitory colonization with no clinical relevance in IC patients and, its percentage can vary according to the studied population.

关键词： Oral cavity immunocompetent Candida fungal colonization.