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黄芪水提物3种效应成分对高糖诱导的内皮细胞损伤的保护作用及机制研究

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北京中医药大学学报 2024年第2期47卷 188-198页

作者：马盼刘汉滢彭美中牛艺婷杨美美徐艺宸刘卓容韩静北京中医药大学中医学院北京102488 证候与方剂基础研究北京市重点实验室证候与方剂基础研究教育部重点实验室(北京中医药大学) 北京中医药大学中医药研究院

目的考察黄芪水提物效应成分对高糖(25 mmol/L)诱导的人脐静脉内皮细胞(HUVEC)损伤的保护作用,并探讨其作用机制。方法给雄性SD大鼠连续灌胃黄芪水提物[20 g/(kg·d)]7 d,分别于末次灌胃后1、2、4 h取血,合并上清,行超高效液相色谱质谱(... 详细信息

目的考察黄芪水提物效应成分对高糖(25 mmol/L)诱导的人脐静脉内皮细胞(HUVEC)损伤的保护作用,并探讨其作用机制。方法给雄性SD大鼠连续灌胃黄芪水提物[20 g/(kg·d)]7 d,分别于末次灌胃后1、2、4 h取血,合并上清,行超高效液相色谱质谱(UPLC⁃MS)分析,筛选黄芪水提物在大鼠体内代谢后的效应成分。将细胞分为正常组、模型组、不同浓度效应成分组及效应成分配伍组。用CCK-8试剂盒检测细胞活力,探讨黄芪水提物效应成分的最佳浓度。通过活性氧(ROS)实验检测细胞氧化损伤情况。酶联免疫吸附测定法检测各组细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)含量;蛋白质印迹法检测核因子E2相关因子(Nrf2)、血红素氧合酶1(HO⁃1)蛋白表达水平。结果芒柄花素、大豆苷元、毛蕊异黄酮是黄芪水提物的效应成分。与正常组比较,模型组细胞活力增加(P<0.05);与模型组比较,芒柄花素各浓度组(26、13、6.5μmol/L)细胞活力降低(P<0.05),大豆苷元高浓度组(0.11μmol/L)细胞活力降低(P<0.05)。与正常组比较,模型组细胞ROS水平升高(P<0.05);与模型组比较,芒柄花素高、中浓度组(26、13μmol/L),大豆苷元不同浓度组(0.11、0.06、0.03μmol/L),毛蕊异黄酮高浓度组(7.00μmol/L)及各配伍组细胞ROS水平均降低(P<0.05)。效应成分配伍对细胞的抑制率高于单个效应成分,且高浓度配伍组抑制率最高。与正常组比较,模型组细胞Nrf2、HO⁃1蛋白表达量降低(P<0.05);与模型组比较,效应成分高浓度配伍组Nrf2、HO⁃1蛋白表达量升高(P<0.05)。与正常组相比,模型组细胞SOD、CAT含量降低(P<0.05)、MDA含量升高(P<0.05);与模型组相比,效应成分高浓度配伍组SOD、CAT含量均升高(P<0.05)、MDA含量降低(P<0.05)。结论黄芪水提物效应成分芒柄花素、大豆苷元及毛蕊异黄酮有保护高糖诱导的HUVEC损伤的作用,其机制可能与调节Nrf2、HO⁃1、SOD、CAT及MDA表达,发挥抗氧化作用有关。

关键词：芒柄花素大豆苷元毛蕊异黄酮配伍黄芪核因子E2相关因子2/血红素氧合酶1信号通路人脐静脉内皮细胞糖尿病视网膜病变

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高职院校在线精品课程建设探究

山东电力高等专科学校学报 2024年第4期27卷 60-65页

作者：杨美美张君君邢恩阳杨玲玲渤海理工职业学院河北沧州061000

通过回顾和分析国家高等职业教育课程建设项目的实施历程,总结了4个建设阶段的重点:精品课程、精品开放课程、精品在线开放课程和在线精品课程,并分析了高等职业教育课程建设的趋势。在明确在线课程概念的基础上,解析了职业教育在线精... 详细信息

通过回顾和分析国家高等职业教育课程建设项目的实施历程,总结了4个建设阶段的重点:精品课程、精品开放课程、精品在线开放课程和在线精品课程,并分析了高等职业教育课程建设的趋势。在明确在线课程概念的基础上,解析了职业教育在线精品课程评选的观测指标。结合在线精品课程评选指标要求和课程建设实践,阐述了课程选择、团队打造、课程设计、平台选择和课程运行等方面的建设策略。

关键词：职业教育在线精品课程精品课程发展课程建设

Effects of dietary Clostridium butyricum and rumen protected fat on meat quality,oxidative stability,and chemical composition of finishing goats

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Journal of Animal Science and Biotechnology 2024年第2期15卷 911-924页

作者： meimei Zhang Zhiyue Zhang Xinlong Zhang Changming Lu Wenzhu yang Xiaolai Xie Hangshu Xin Xiaotan Lu Mingbo Ni Xinyue yang Xiaoyang Lv Peixin Jiao College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030People’s Republic of China Lethbridge Research and Development Centre Lethbridge T1J 4B1Canada School of Agriculture and Food Sciences The University of QueenslandGattonQLD 4343Australia International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement Yangzhou 225009China

Background Clostridium butyricum(CB)is a probiotic that can regulate intestinal microbial composition and improve meat *** protected fat(RPF)has been shown to increase the dietary energy density and provide essential fatty ***,it is still unknown whether dietary supplementation with CB and RPF exerts beneficial effects on growth performance and nutritional value of goat *** study aimed to investigate the effects of dietary CB and RPF supplementation on growth performance,meat quality,oxidative stability,and meat nutritional value of finishing ***-two goats(initial body weight,20.5±0.82 kg)were used in a completely randomized block design with a 2 RPF supplementation(0 vs.30 g/d)×2 CB supplementation(0 vs.1.0 g/d)factorial treatment *** experiment included a 14-d adaptation and 70-d data and sample collection *** goats were fed a diet consisted of 400 g/kg peanut seedling and 600 g/kg corn-based concentrate(dry matter basis).Result Interaction between CB and RPF was rarely observed on the variables measured,except that shear force was reduced(P<0.05)by adding CB or RPF alone or their combination;the increased intramuscular fat(IMF)content with adding RPF was more pronounced(P<0.05)with CB than without CB *** pH24h(P=0.009),a*values(P=0.007),total antioxidant capacity(P=0.050),glutathione peroxidase activities(P=0.006),concentrations of 18:3(P<0.001),20:5(P=0.003)and total polyunsaturated fatty acids(P=0.048)were increased,whereas the L*values(P<0.001),shear force(P=0.050)and malondialdehyde content(P=0.044)were decreased by adding ***,CB supplementation increased essential amino acid(P=0.027),flavor amino acid(P=0.010)and total amino acid contents(P=0.024)as well as upregulated the expression of lipoprotein lipase(P=0.034)and peroxisome proliferator-activated receptorγ(PPARγ)(P=0.012),and downregulated the expression of stearoyl-CoA desaturase(SCD)(P=0.034).The RPF supplementation increased dry

关键词： Chemical composition Clostridium butyricum Goats Meat quality Oxidative stability Rumen protected fat

Polystyrene nanoplastics induce glycolipid metabolism disorder via NF-κB and MAPK signaling pathway in mice

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Journal of Environmental Sciences 2024年第3期137卷 553-566页

作者： Xingpei Fan Xiaoyan Li Jiaxin Li Yuxia Zhang Xiangjuan Wei Hailong Hu Boya Zhang Haining Du meimei Zhao Ruijiao Zhu Daqian yang Yuri Oh Ning Gu School of Life Science and Technology Harbin Institute of TechnologyHarbin 150001China State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150006China Department of Medicine Renal Electrolyte and Hypertension DivisionDepartment of GeneticsPerelman School of MedicineUniversity of PennsylvaniaPhiladelphia 19104USA Faculty of Education Wakayama UniversityWakayama 640-8441Japan

Nanoplastics-induced developmental and reproductive toxicity,neurotoxicity and immunotoxicity are a focus of widespread ***,the effects of nanoplastics(NPs)on glycolipid metabolism and the precise underlying mechanisms are unclear at ***,we showed that oral administration of polystyrene nanoparticles(PS-NPs)disrupts glycolipid metabolism,with reactive oxygen species(ROS)identified as a potential key signaling *** PS-NPs treatment,excessive production of ROS induced the infammatory response and activated the antioxidant pathway through nuclear factor-erythroid factor 2-related factor *** activation of nuclear factor-κB(NFκB)signaling pathway induced the phosphorylation of the mitogen-activated protein kinases(MAPK)signaling pathway,which induced the activation of extracellular regulated kinases(ERK)and *** activation of the MAPK signaling proteins induced high continued phosphorylation of insulin receptor substrate-1,in turn,leading to decreased protein kinase B(Akt)activity,which weakened the sensitivity of liver cells to insulin signals and induced insulin *** parallel,phosphorylation of Akt led to loss of control of Fo XO1,a key gene of gluconeogenesis,activating transcription of glucose-6-phosphatase(G6PC)and phosphoenolpyruvate carboxykinase(PEPCK)in a manner dependent on PGC1α.Moreover,the activated ERK promoted lipid accumulation through ERK-PPARγ***,sterol regulatory element-binding protein-1 and levels of its downstream lipogenic enzymes,ACC-1,were *** treatment with the antioxidant resveratrol,PS-NPs-induced glucose and lipid metabolic disorders were improved by inhibiting ROS-induced activation of NFκB and MAPK signaling pathway in *** on above,PS-NPs exposure disrupts glycolipid metabolism in mice,with ROS identified as a potential key signaling molecule.

关键词： Nanoplastics Resveratrol Hyperglycemia Lipid metabolism Infammatory response Reactive oxygen species(ROS)

Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157:H7 through transcriptomic analysis

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Food Science and Human Wellness 2024年第2期13卷 736-747页

作者： Yilin Lin Ruifei Wang Xiaoqing Li Keren Agyekumwaa Addo meimei Fang Yehui Zhang Yigang Yu School of Food Sciences and Engineering South China University of TechnologyGuangzhou 510640China Sericulture&Agri-food Research Institute Guangdong Academy of Agricultural Sciences Key Laboratory of Functional FoodsMinistry of Agriculture and Rural AffairsGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Research Center of Food Safety and Detection College of Food Science and EngineeringSouth China University of TechnologyGuangzhou 510640China

Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in *** previous found that a combination of kojic acid and tea polyphenols exhibited better activity against *** O157:H7 than using either *** study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene *** functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against *** O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic ***,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid *** results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against *** O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.

关键词： Kojic acid Tea polyphenols Antibacterial mechanism Escherichia coli O157:H7 RNA-seq

Integrated neural tracing and in-situ barcoded sequencing reveals the logic of SCN efferent circuits in regulating circadian behaviors

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Science China(Life Sciences) 2024年第3期67卷 518-528页

作者： meimei Liao Xinwei Gao Chen Chen Qi Li Qingchun Guo He Huang Erquan Zhang Dapeng Ju College of Biological Sciences China Agriculture UniversityBeijing 100193China National Institute of Biological Sciences(NIBS) Beijing 102206China Chinese Institute for Brain Research Beijing 102206China School of Biomedical Engineering Capital Medical UniversityBeijing 100069China Department of Anesthesiology The Second Affiliated HospitalChongqing Medical UniversityChongqing 401336China Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua UniversityBeijing 102206China

The circadian clock coordinates rhythms in numerous physiological processes to maintain organismal homeostasis. Since the suprachiasmatic nucleus(SCN) is widely accepted as the circadian pacemaker, it is critical to understand the neural mechanisms by which rhythmic information is transferred from the SCN to peripheral clocks. Here, we present the first comprehensive map of SCN efferent connections and suggest a molecular logic underlying these projections. The SCN projects broadly to most major regions of the brain, rather than solely to the hypothalamus and thalamus. The efferent projections from different subtypes of SCN neurons vary in distance and intensity, and blocking synaptic transmission of these circuits affects circadian rhythms in locomotion and feeding to different extents. We also developed a barcoding system to integrate retrograde tracing with in-situ sequencing, allowing us to link circuit anatomy and spatial patterns of gene expression. Analyses using this system revealed that brain regions functioning downstream of the SCN receive input from multiple neuropeptidergic cell types within the SCN, and that individual SCN neurons generally project to a single downstream brain *** map of SCN efferent connections provides a critical foundation for future investigations into the neural circuits underlying SCNmediated rhythms in physiology. Further, our new barcoded tracing method provides a tool for revealing the molecular logic of neuronal circuits within heterogeneous brain regions.

关键词： circadian rhythms SCN output circuit neural tracing barcoded GFP in situ sequencing

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基于莫比乌斯陀螺矢量空间的双曲正定核

模式识别与人工智能 2023年第9期36卷 842-855页

作者：杨梅梅方鹏飞朱士鹏薛晖东南大学计算机科学与工程学院南京211189 东南大学新一代人工智能技术与交叉应用教育部重点实验室南京211189

层次结构数据广泛存在于各类机器学习场景中,双曲空间能够以极低的失真编码层次结构数据,引入核方法后,可进一步提高双曲空间的表征能力.然而,现有的双曲核仍然存在自适应能力较低或数据失真的缺陷.为了解决这些问题,文中提出基于莫比... 详细信息

层次结构数据广泛存在于各类机器学习场景中,双曲空间能够以极低的失真编码层次结构数据,引入核方法后,可进一步提高双曲空间的表征能力.然而,现有的双曲核仍然存在自适应能力较低或数据失真的缺陷.为了解决这些问题,文中提出基于莫比乌斯陀螺矢量空间的双曲正定核方法.利用莫比乌斯陀螺矢量空间与庞加莱模型之间的关系,构造莫比乌斯径向基核.具体使用莫比乌斯陀螺距离代替欧几里得距离,构造莫比乌斯高斯核和莫比乌斯拉普拉斯核,并进一步证明核函数的正定性.另外,将该核函数从复空间转换到实空间上,更适用于大多数机器学习任务.在多组真实的社交网络数据集上的实验验证文中方法的有效性.

关键词：双曲几何双曲核函数庞加莱模型正定核莫比乌斯陀螺矢量空间

混响环境下移动机器人语音控制方法及系统实现

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仪器仪表学报 2019年第11期40卷 165-171页

作者：李艳生刘园张毅杨美美重庆邮电大学先进制造工程学院重庆400065

为了满足移动机器人交互控制多样性需求,提升移动机器人的语音控制性能,设计基于语音的移动机器人控制系统。通过对控制信号传递过程和机器人使用环境中语音信号噪音源分析,制定出移动机器人语音控制系统组成方案,给出前端语音识别部分... 详细信息

为了满足移动机器人交互控制多样性需求,提升移动机器人的语音控制性能,设计基于语音的移动机器人控制系统。通过对控制信号传递过程和机器人使用环境中语音信号噪音源分析,制定出移动机器人语音控制系统组成方案,给出前端语音识别部分实现的主要流程,重点对语音增强的解混响算法进行设计。充分利用语音潜在的谱特征,将非负矩阵分解和深度神经网络结合提出一种解混响算法,先通过矩阵分解得到语音信号特征,再生成特征矢量来训练激活函数,降低深度神经网络模型的训练复杂度,经过对比分析表明该算法对解决语音混响问题具有优势。编写控制软件并嵌入语音识别算法,搭建工业移动机器人语音控制平台来验证语音控制系统有效性,在混响环境下不同人对机器人多个动作进行语音控制实验,结果表明该系统能够实现移动机器人语音控制,所提出的语音识别方法可使机器人在0.3、0.6和0.9 s的混响条件下动作正确平均执行率分别能达到96%、95%和93%以上。

关键词：移动机器人控制系统深度神经网络语音识别

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河北省现代服务业产业关联分析

科技管理研究 2015年第1期35卷 122-126,140页

作者：陈红梅杨美美燕山大学经济管理学院河北秦皇岛066004

产业关联是分析国民经济各部门内在经济联系规律的重要分析指标,认清不同产业间的关联程度对地方经济发展及政策的制定具有重要意义。运用相关分析研究方法,计算出河北省现代服务业中6个产业间的相关及偏相关系数,对河北省现代服务业间... 详细信息

产业关联是分析国民经济各部门内在经济联系规律的重要分析指标,认清不同产业间的关联程度对地方经济发展及政策的制定具有重要意义。运用相关分析研究方法,计算出河北省现代服务业中6个产业间的相关及偏相关系数,对河北省现代服务业间的经济关联程度进行判断分析,选择确定在经济发展中起重要作用的主导型产业。数据结果发现:河北省现代服务业中各产业间经济互动性较强,金融业处于河北省现代服务业的主导地位,批发与零售业经济作用最为薄弱,从而论证相关分析在产业关联研究的可行性,同时为河北省产业优化发展提供理论支持。

关键词：河北省现代服务业产业关联度相关系数偏相关系数