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Acute kidney injury in acute-on-chronic liver failure is different from in decompensated cirrhosis

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World Journal of Gastroenterology 2018年第21期24卷 2300-2310页

作者： Qun-Qun Jiang mei-fang han Ke Ma Guang Chen Xiao-Yang Wan Semvua Bukheti Kilonzo Wen-Yu Wu Yong-Li Wang Jie You Qin Ning Department and Institute of Infectious Disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

AIM To evaluate the differences in acute kidney injury(AKI) between acute-on-chronic liver failure(ACLF) and decompensated cirrhosis(DC) patients. METHODS During the period from December 2015 to July 2017, 280 patients with hepatitis B virus(HBV)-related ACLF(HBV-ACLF) and 132 patients with HBV-related DC(HBV-DC) who were admitted to our center were recruited consecutively into an observational study. Urine specimens were collected from all subjects and the levels of five urinary tubular injury biomarkers were detected,including neutrophil gelatinase-associated lipocalin(NGAL), interleukin-18(IL-18), liver-type fatty acid binding protein(L-FABP), cystatin C(CysC), and kidney injury molecule-1(KIM-1). Simultaneously, the patient demographics, occurrence and progression of AKI, and response to terlipressin therapy were recorded. All patients were followed up for 3 mo or until death after enrollment. RESULTS AKI occurred in 71 and 28 of HBV-ACLF and HBV-DC patients, respectively(25.4% vs 21.2%, P = 0.358). Among all patients, the levels of four urinary biomarkers(NGAL, CysC, L-FABP, IL-18) were significantly elevated in patients with HBV-ACLF and AKI(ACLF-AKI), compared with that in patients with HBV-DC and AKI(DC-AKI) or those without AKI. There was a higher proportion of patients with AKI progression in ACLF-AKI patients than in DC-AKI patients(49.3% vs 17.9%, P = 0.013). Fortythree patients with ACLF-AKI and 19 patients with DC-AKI were treated with terlipressin. The response rate of ACLFAKI patients was significantly lower than that of patients with DC-AKI(32.6% vs 57.9%, P = 0.018). Furthermore, patients with ACLF-AKI had the lowest 90 d survival rates among all groups(P han that in DC patients.

关键词： Decompensated cirrhosis Acute-on-chronic liver failure Acute kidney injury Biomarker Etiology Treatment Prognosis

Immune response pattern varies with the natural history of chronic hepatitis B

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World Journal of Gastroenterology 2019年第16期25卷 1950-1963页

作者： Wen-Tao Wang Xue-Qi Zhao Gui-Ping Li Yi-Zhi Chen Lin Wang mei-fang han Wei-Na Li Tao Chen Guang Chen Dong Xu Qin Ning Xi-Ping Zhao Department of Infectious Diseases Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Huazhong University of Science and Technology Department of Heart Function Examination Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Pathophysiology Hubei University of Medicine

BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant(IT), immune active(IA), inactive carrier(IC) and hepatitis B envelope antigen(HBeAg)-negative hepatitis(ENEG).AIM To investigate the immune status of natural killer(NK) and T cells in different phases of chronic hepatitis *** The frequency, phenotype and function of circulating NK cells, as well as nonantigen-specific and hepatitis B virus(HBV)-specific T cell responses were detected by flow cytometry in healthy and HBV-infected *** The ability of NK cells to produce IFN-γ was markedly attenuated in HBVinfected patients overall but was less compromised in IC patients. Patients in the IT and IA phases also displayed significantly lower TNF-α production compared to healthy subjects. NK cells were phenotypically activated in the IA and ENEGphases, as evidenced by the upregulation of NKp44 in CD56^(bright) NK cells and CD69 in CD56^(dim) NK cells. Furthermore, global T-cells from the ENEG phase displayed a proinflammatory cytokine profile with upregulated IFN-γ and TNF-αexpression, while this profile was suppressed in IT and IA patients. Finally, core and S antigen-specific T cell responses were significantly stronger after in vitro expansion in the IC phase compared to other *** Our findings demonstrate the changes in immune response pattern during the natural history of HBV infection. Both NK and T cells are functionally impaired in the IT and IA phases. With the spontaneous clearance of HBeAg and hepatitis B surface antigen decline, NK cell cytokine production and HBV-specific T responses are partially restored in IC phase, and the ENEG phase is dominated by nonantigen-specific T cell responses.

关键词： Chronic hepatitis Hepatitis B virus Natural killer cells Global-T cells Virusspecific T cells Natural history Heterogeneity

Real-time fluorescent quantitative PCR assay for measuring cytomegalovirus DNA load in patients after haematopoietic stem cell transplantation

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Chinese Medical Journal 2006年第10期119卷 871-874页

作者： FAN Jun MA Wei-hang YANG mei-fang XUE han GAO Hai-nü LI Lan-juan Key Laboratory of Ministry Infectious Disease Institute ofInfectious Diseases First Affiliated Hospital Zhejiang UniversitySchool of Medicine Hangzhou 310003 China

Cytomegalovirus （CMV） infection is a major and often deadly complication of haematopoietic stem cell （HSC） transplantation. Successful preemptive CMV therapy in transplant patients depends on the availability of sensitive, specific, and timely diagnostic tests for CMV infections. The pp65 antigenemia assay has been used for this purpose with considerable success but has disadvantages of being time-consuming and labor-intensive .

关键词： haematopoietic stem cell transplantation cytomegalovirus real-time systems polymerase chain reaction

Preparation and applications of calcium ferrite as a functional material:A review

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 292-310页

作者： Xiuli han Bowen Duan Lei Liu Shilong fang Weiwei Wang College of Mining Engineering North China University of Science and TechnologyTangshan 063210China Collaborative Innovation Center of Green Development and Ecological Restoration of Mineral Resources of Hebei Province Tangshan 063210China Key Laboratory of Mining Development and Security Technology of Hebei Province Tangshan 063210China

Calcium ferrite(CF)is recognized as a potential green and efficient functional material because of its advantages of magnetism,electrochemistry,catalysis,and biocompatibility in the fields of materials chemistry,environmental engineering,and ***-fore,the obtained research results need to be systematically summarized,and new perspectives on CF and its composite materials need to be *** on the presented studies of CF and its composite materials,the types and structures of the crystal are *** addition,the current application technologies and theoretical mechanisms with various properties in different fields are ***,the various preparation methods of CF and its composite materials are elaborated in *** importantly,the advantages and disadvantages of the synthesis methods of CF and its composite materials are discussed,and the existing problems and emerging challenges in practical production are ***,the key future research directions of CF and its composite materials have been prospected from the potential application technologies to provide references for its synthesis and efficient utilization.

关键词： calcium ferrite mineral functional materials preparation application perspectives

Microstructural evolution during the progressive transformation-induced plasticity effect in a Fe-0.1C-5Mnmedium manganese steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 369-379页

作者： mei Zhang Wenhao Li Yangfei Chen Yang Jiang Xiaofei Guo han Dong School of Materials Science and Engineering Shanghai UniversityShanghai 200444China State Key Laboratory of Advanced Special Steel Shanghai UniversityShanghai 200444China

The microstructural evolution of a cold-rolled and intercritical annealed medium-Mn steel(Fe-0.10C-5Mn)was investigated during uniaxial tensile ***-situ observations under scanning electron microscopy,transmission electron microscopy,and X-ray diffraction analysis were conducted to characterize the progressive transformation-induced plasticity process and associated fracture initiation *** findings were discussed with the local strain measurements via digital image *** results indicated that Lüders band formation in the steel was limited to 1.5%strain,which was mainly due to the early-stage martensitic phase transformation of a very small amount of the less stable large-sized retained austenite(RA),which led to localized stress concentrations and strain hardening and further retardation of *** small-sized RA exhibited high stability and progressively transformed into martensite and contributed to a stably extended Portevin-Le Chatelier *** volume fraction of RA gradually decreased from 26.8%to 8.2%prior to *** the late deformation stage,fracture initiation primarily occurred at the austenite/martensite and ferrite/martensite interfaces and the ferrite phase.

关键词： medium-Mn steel retained austenite progressive transformation-induced plasticity effect local strain fracture initiation

Aβ25-35诱导PC12细胞周期变化与凋亡的关系

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四川大学学报（医学版） 2008年第1期39卷 30-33页

作者：谢朝阳梅寒芳祝其锋广东医学院生物化学与分子生物学研究所

目的探讨β-淀粉样肽25—35（βamyloid peptide-25-35，Aβ25-35）诱导体外去血清培养的PC12细胞周期变化与凋亡的关系。方法用常规的去血清培养使细胞同步于G0期，采用终浓度为0-45μmol／L Aβ25-35处理PC12细胞24h，用MTT比色法分... 详细信息

目的探讨β-淀粉样肽25—35（βamyloid peptide-25-35，Aβ25-35）诱导体外去血清培养的PC12细胞周期变化与凋亡的关系。方法用常规的去血清培养使细胞同步于G0期，采用终浓度为0-45μmol／L Aβ25-35处理PC12细胞24h，用MTT比色法分析细胞存活率；Hoechst33258荧光染色观察细胞核凋亡的形态学改变；DNA电泳观察细胞凋亡时特异性梯状条带（DNA-Ladder）；流式细胞仪检测分析细胞周期的改变与凋亡的时间关系。结果随着Aβ25-35剂量的增加，PC12细胞存活率降低（P〈0．01）；用25μmol／L Aβ25-35处理PC12细胞12、24h，荧光染色结果显示24h出现典型的凋亡，表现为核固缩、核碎裂；DNA电泳结果显示凋亡特异性梯状条带；流式细胞仪分析表明去血清培养24h可使约90％PC12细胞停滞于G0／G1期，25μmol／L Aβ25-35诱导组8、16、24h与对照组比较，S期细胞比例增加（P〈0．01），16h后细胞凋亡率增加（P〈0．01），可见明显的亚二倍体峰（Ap峰）。结论Aβ25-35降低去血清培养的PC12细胞存活率，诱导同步化于G0／G1的PC12细胞重新进入细胞周期并随之出现凋亡，提示Aβ25-35诱导的PC12细胞凋亡可能与细胞周期紊乱有关。

关键词： β-淀粉样肽25-35 PC12细胞细胞周期凋亡

原花青素对Aβ_(25-35)诱导PC12细胞凋亡的影响及其机制的研究

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中国药理学通报 2005年第2期21卷 227-231页

作者：梅寒芳谢朝阳祝其锋广东医学院生物化学与分子生物学研究所广东湛江524023

目的探讨原花青素(Procyanidins，PC)对β淀粉样肽25～35(β amyloid peptide25-35，Aβ25-35)诱导PCI2细胞凋亡的影响及其机制。方法采用MTT比色法分析细胞存活率，Hoechst 33258-P1荧光染色法检测凋亡，RT-PCR检测P53和bcl-2基因mRN... 详细信息

目的探讨原花青素(Procyanidins，PC)对β淀粉样肽25～35(β amyloid peptide25-35，Aβ25-35)诱导PCI2细胞凋亡的影响及其机制。方法采用MTT比色法分析细胞存活率，Hoechst 33258-P1荧光染色法检测凋亡，RT-PCR检测P53和bcl-2基因mRNA表达，Western blot检测P53和Bcl-2蛋白表达。结果不同剂量PC预处理PC12细胞1h可剂量依赖性对抗Aβ25-35引起的凋亡，提高细胞的存活率，减少Aβ25-35引起的核固缩，凝聚和碎裂，降低P53 mRNA表达以及P53蛋白表达，增加bcl-2mRNA表达以及Bcl-2蛋白表达。结论 PC可剂量依赖性对抗Aβ25-35对PC12细胞的毒性作用，其机制可能与下调凋亡基因P53和上调抗凋亡基因Bcl-2表达有关。

关键词：原花青素 Aβ25-35 PC12细胞凋亡 P53 bcl-2

rhIL-10对脂多糖攻击小鼠血及肝IL-6和TNF-α含量的影响

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中国病理生理杂志 2005年第6期21卷 1207-1209页

作者：杨红罗少洪梅寒芳李春梅广东药学院基础学院广东广州510224

目的:研究重组人白细胞介素-10(rhIL-10)对脂多糖(LPS)诱导的血、肝IL-6和TNF-α炎症介质含量变化的影响。方法:小鼠腹腔注射LPS建立炎症模型,并同时静脉注射不同剂量的rhIL-10,ELISA法测定12h、24h、48h和72h肝组织和血清IL-6和TNF-α... 详细信息

目的:研究重组人白细胞介素-10(rhIL-10)对脂多糖(LPS)诱导的血、肝IL-6和TNF-α炎症介质含量变化的影响。方法:小鼠腹腔注射LPS建立炎症模型,并同时静脉注射不同剂量的rhIL-10,ELISA法测定12h、24h、48h和72h肝组织和血清IL-6和TNF-α的含量。结果:注射rhIL-10后12h,肝组织和血清IL-6、TNF-α水平开始下调,24-48h抑制作用最明显(P<0.05),72h后抑制作用减弱,且呈剂量效应关系。结论:利用基因工程技术制备的重组人白细胞介素10(rhIL-10)显著下调肝组织和血清IL-6和TNF-α的水平。

关键词：白细胞介素10 白细胞介素6 肿瘤坏死因子

Brain region-specific roles of brain-derived neurotrophic factor in social stress-induced depressive-like behavior

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Neural Regeneration Research 2025年第1期20卷 159-173页

作者： Man han Deyang Zeng Wei Tan Xingxing Chen Shuyuan Bai Qiong Wu Yushan Chen Zhen Wei Yufei mei Yan Zeng Brain Science and Advanced Technology Institute Wuhan University of Science and TechnologyWuhanHubei ProvinceChina Geriatric Hospital Affiliated to Wuhan University of Science and Technology WuhanHubei ProvinceChina School of Public Health Wuhan University of Science and TechnologyWuhanHubei ProvinceChina

Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral *** studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region–specific,particularly involving the corticolimbic system,including the ventral tegmental area,nucleus accumbens,prefrontal cortex,amygdala,and *** how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress *** this review,we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of *** focused on associated molecular pathways and neural circuits,with special attention to the brain-derived neurotrophic factor–tropomyosin receptor kinase B signaling pathway and the ventral tegmental area–nucleus accumbens dopamine *** determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature,severity,and duration of stress,especially in the above-mentioned brain regions of the corticolimbic ***,BDNF might be a biological indicator regulating stress-related processes in various brain regions.

关键词： amygdala chronic mild stress chronic social defeat stress corticolimbic system depression hippocampus medial prefrontal cortex nucleus accumbens social stress models ventral tegmental area