检索结果-南通市图书馆

Insufficient TRPM5 Mediates Lipotoxicity-induced Pancreaticβ-cell Dysfunction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2024年第2期44卷 346-354页

作者： Kai-yuan WANG Shi-mei wu Zheng-jian YAO Yun-xia ZHU Xiao HAN Key Laboratory of Human Functional Genomics of Jiangsu Province Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjing 211166China

Objective:While the reduction of transient receptor potential channel subfamily M member 5(TRPM5)has been reported in islet cells from type 2 diabetic(T2D)mouse models,its role in lipotoxicity-induced pancreaticβ-cell dysfunction remains *** study aims to study its ***:Pancreas slices were prepared from mice subjected to a high-fat-diet(HFD)at different time points,and TRPM5 expression in the pancreaticβcells was examined using immunofluorescence ***-stimulated insulin secretion(GSIS)defects caused by lipotoxicity were mimicked by saturated fatty acid palmitate(Palm).Primary mouse islets and mouse insulinoma MIN6 cells were treated with Palm,and the TRPM5 expression was detected using qRT-PCR and Western ***-induced GSIS defects were measured following siRNA-based Trpm5 *** detrimental effects of Palm on primary mouse islets were also assessed after overexpressing Trpm5 via an adenovirus-derived Trpm5(Ad-Trpm5).Results:HFD feeding decreased the mRNA levels and protein expression of TRPM5 in mouse pancreatic *** reduced TRPM5 protein expression in a time-and dose-dependent manner in MIN6 *** also inhibited TRPM5 expression in primary mouse *** of Trpm5 inhibited insulin secretion upon high glucose stimulation but had little effect on insulin *** of Trpm5 reversed Palm-induced GSIS defects and the production of functional maturation molecules unique toβ***:Our findings suggest that lipotoxicity inhibits TRPM5 expression in pancreaticβcells both in vivo and in vitro and,in turn,drivesβ-cell dysfunction.

关键词： type 2 diabetes β-cell dysfunction lipotoxicity TRPM5

Privacy-Preserving Multi-Keyword Fuzzy Adjacency Search Strategy for Encrypted Graph in Cloud Environment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2024年第3期78卷 3177-3194页

作者： Bin wu Xianyi Chen Jinzhou Huang Caicai Zhang Jing Wang Jing Yu Zhiqiang Zhao Zhuolin mei School of Computer and Big Data Science Jiujiang UniversityJiujiang332005China Jiujiang Key Laboratory of Network and Information Security Jiujiang332005China School of Computer and Software Nanjing University of Information Science&TechnologyNanjing210044China School of Computer Engineering Hubei University of Arts and ScienceXiangyang441053China School of Modern Information Technology Zhejiang Institute of Mechanical and Electrical EngineeringHangzhou310053China Information Center Jiangxi Changjiang Chemical Co.Ltd.Jiujiang332005China School of Mathematics and Computer Science Ningxia Normal UniversityGuyuan756099China

In a cloud environment,outsourced graph data is widely used in companies,enterprises,medical institutions,and so *** owners and users can save costs and improve efficiency by storing large amounts of graph data on cloud *** on cloud platforms usually have some subjective or objective attacks,which make the outsourced graph data in an insecure *** issue of privacy data protection has become an important obstacle to data sharing and *** to query outsourcing graph data safely and effectively has become the focus of *** query is a basic and frequently used operation in graph,and it will effectively promote the query range and query ability if multi-keyword fuzzy search can be supported at the same *** work proposes to protect the privacy information of outsourcing graph data by encryption,mainly studies the problem of multi-keyword fuzzy adjacency query,and puts forward a *** our scheme,we use the Bloom filter and encryption mechanism to build a secure index and query token,and adjacency queries are implemented through indexes and query tokens on the cloud *** proposed scheme is proved by formal analysis,and the performance and effectiveness of the scheme are illustrated by experimental *** research results of this work will provide solid theoretical and technical support for the further popularization and application of encrypted graph data processing technology.

关键词： Privacy-preserving adjacency query multi-keyword fuzzy search encrypted graph

Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2024年第3期3卷 30-42页

作者： Borui Su Dong Gao Nini Xin Kai wu mei Yang Shichao Jiang Yusheng Zhang Jie Ding Chengheng wu Jing Sun Dan Wei Hongsong Fan Zhenzhen Guo National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengduSichuan 610064China Institute of Regulatory Science for Medical Devices Sichuan UniversityChengduSichuan 610064China Department of Gastroenterology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610o72China

Lipid droplets(LDs)participating in various cellular activities and are increasingly being *** imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield(PLQY),poor photostability and tedious washing ***,a novel yellow-emissive carbon dot(OT-cD)has been synthesized conveniently with high PLQY up to 90%.Besides,OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2,which is much higher than most LDs *** characters enable OT-CD high brightness,stable and wash-free LDs probing,and feasible for in vivo ***,detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded,including ferroptosis and other diseases ***,fast whole imaging of zebrafish and identifed LD enrichment in injured liver indicate its further feasibility for in vivo *** contrast to the reported studies to date,this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes,combing the advantages of easy and high-yield production,as well as robust brightness and stability for long-term imaging,facilitating investigations into organelle interactions and LD-associated diseases.

关键词： lipid droplet probe carbon dots fluorescence imaging wash-free dynamic monitoring

Analysis on the causes of massive stranding of Yellow Sea green tide on Lianyungang and Rizhao coasts in 2022

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2024年第3期42卷 816-830页

作者： Lingjuan wu Juan HUANG Yi DING Guiyan LIU Shuai HUANG Song GAO Chao YUAN Jiangling XU Ping wu Rui HUANG Ruobing WEN Jie mei North China Sea Marine Forecasting and Hazard Mitigation Center Ministry of Natural ResourcesQingdao 266061China Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation Qingdao 266061China National Engineering Laboratory for Integrated Aero-Space-Ground-Ocean Big Data Application Technology Xi’an 710129China East China Sea Marine Forecasting and Hazard Mitigation Center Ministry of Natural ResourcesShanghai 200136China North China Sea Ecological Center Ministry of Natural ResourcesQingdao 266033China

In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less ***,the largest amount of seaweed biomass has been washed ashore on Lianyungang and Rizhao coasts since *** studied the development pattern of Yellow Sea green tide in 2022,and analyzed the key environmental factors on the growth and drifting,then discussed the possible reasons that resulted in the massive stranding of green tide biomass in Lianyungang and *** show under the combined influence of the east to southeast winds and currents with shoreward anomalies,green tide drifted to the coastal waters between Shandong and Jiangsu provinces and the distribution areas located westward compared with previous years(2008–2021).Floating *** rafts from the coastal waters of Binhai and Sheyang drifted continuously into the coastal waters of Lianyungang and Rizhao,providing important supplements for Yellow Sea green *** green tide in 2022 distributed close to the coastal waters,the abundant nutrients might support their continuous high growth *** addition,the amount of rainfall around Shandong Peninsula from late June to early July were significantly higher than in previous years,which might promote the development of green tide to some extent.

关键词： Yellow Sea green tide stranding biomass development pattern environmental factor

Animal models of hepatitis E infection: Advances and challenges

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 171-180页

作者： Ze Xiang Xiang-Lin He Chuan-wu Zhu Jia-Jia Yang Lan Huang chun Jiang Jian wu Chinese Consortium for the Study of Hepatitis E(CCSHE) Zhejiang University School of Medicine Hangzhou 310030China Department of Infectious Diseases The Fifth People’s Hospital of SuzhouSuzhou 215007China Department of Infection Management The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China Department of Clinical Laboratory The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China 不详

Hepatitis E virus(HEV)is one of the leading causes of acute viral hepatitis *** most of HEV infections are asymptomatic,some patients will develop the symptoms,especially pregnant women,the elderly,and patients with preexisting liver diseases,who often experience anorexia,nausea,vom-iting,malaise,abdominal pain,and *** infection may become chronic in immunosuppressed *** addition,HEV infection can also cause several extrahepatic *** exists in a wide range of hosts in nature and can be transmitted across ***,animals susceptible to HEV can be used as *** establishment of animal models is of great significance for studying HEV transmission,clinical symptoms,extrahepatic manifestations,and therapeutic strategies,which will help us understand the pathogenesis,prevention,and treatment of hepatitis *** review summarized the animal models of HEV,including pigs,monkeys,rabbits,mice,rats,and other *** each animal species,we provided a concise summary of the HEV genotypes that they can be infected with,the cross-species transmission pathways,as well as their role in studying extrahepatic manifestations,prevention,and treatment of HEV *** advantages and disadvantages of these animal models were also *** review offers new perspectives to enhance the current understanding of the research landscape surrounding HEV animal models.

关键词： Hepatitis E virus Animal models Pathogenesis Prevention Treatment

Effect of cardiac rehabilitation care after coronary intervention on cardiac function recovery and negative mood in patients with myocardial infarction

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第1期12卷 59-67页

作者： Ming Yang Yuan-Tao Huang Xi-Wen Hu chun-Ling wu Division of Cardiovascular First Ward Departments of Internal MedicineLiyuan HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430077Hubei ProvinceChina Division of Cardiovascular First Ward Departments of Internal MedicineJianli People's HospitalJianli 433300Hubei ProvinceChina Department of Integrated Traditional Chinese and Western Medicine People's Hospital of Dongxihu DistrictWuhan 430040Hubei ProvinceChina

BACKGROUND Cardiovascular disease,particularly myocardial infarction(MI)profound impact on patients'quality of life and places a substantial burden on the healthcare and economy *** in medical technology have led to the emer-gence of coronary intervention as an essential method for treating *** To assess the effects of cardiac rehabilitation care on cardiac function recovery and negative emotions in MI after coronary *** This study included a total of 180 patients with MI during the period from June 2022 to July *** patients were divided into two groups:An observation group,which receiving cardiac rehabilitation care;a control group,which re-ceiving conventional *** comparing multiple observation indicators such as cardiac function indicators,blood pressure,exercise tolerance,occurrence of adverse cardiac events,and negative emotion scores between the two groups of *** the data were analyzed and compared between two *** There were 44 males and 46 females in the observation group with an average age of 36.26±9.88 yr;there were 43 males and 47 females in the control group,with an average age of 40.87±10.5 *** receiving the appropriate postoperative nursing measures,the results of the observation group showed significant improvement in several indicators compared with the control *** of cardiac function,such as left ventricular end-diastolic internal diameter and left ventricular ejection fraction were significantly better in the observation group than in the control group(P<0.05).Exercise endurance assessment showed that the 6-minute walking test distance was significantly increased in the patients of the observation group(P<0.01).In addition,the incidence of adverse cardiac events was significantly lower in the observation group,and negative mood scores were significantly reduced(P<0.05).CONCLUSION Cardiac rehabilitation care after coronary intervention has a significant positive

关键词： Myocardial infarction Coronary artery intervention Cardiac rehabilitation Cardiac function recovery Negative emotions

Magnitude and direction of temperature variability affect hospitalization for myocardial infarction and stroke:population-based evidence from Guangzhou,China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2024年第3期18卷 1-11页

作者： Zhou Yang Murui Zheng Ze-Lin Yan Hui Liu Xiangyi Liu Jie-Qi Jin Jiagang wu chun-Quan Ou State Key Laboratory of Organ Failure Research Department of BiostatisticsGuangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou 510515China Guangzhou Center for Disease Control and Prevention Guangzhou 510440China

Relationships between nonoptimal temperatures and cardiovascular disease(CVD)mortality have been well ***,evidence of the association between temperature variability(TV)and CVD morbidity is *** study aimed to quantify the risk and burden of CVD-related hospitalization associated with the magnitude and direction of *** on meteorology and population-based hospitalizations for myocardial infarction(MI)and stroke were collected in Guangzhou,China,from 2013 to *** temperature variability(HTV)was measured as the standard deviation of hourly temperature records over specific exposure *** direction(upward or downward)of HTV was defined as the average daily mean temperature change relative to that of the previous day during the exposure ***-Poisson regression was applied to assess the impact of HTV after adjusting for the daily mean temperature,and the hospitalization fractions attributable to HTV were calculated.A 1℃-increase in HTV was significantly associated with a 2.24%and 1.72%increase in hospitalizations for MI and hemorrhagic stroke(HS)at lag 0–1 d,respectively,and a 1.55%increase in hospitalizations for ischemic stroke(IS)at lag 0–3 *** the study period,5.99%,4.64%,and 4.53%of MI,HS,and IS hospitalizations,respectively,were attributable to *** upward TV exerts acute effects on CVD hospital admissions,whereas the impact of downward TV generally *** findings highlight the importance of the magnitude and direction of temperature fluctuations,in addition to the mean level,in assessing the adverse health impacts of temperature variations.

关键词： Hourly temperature variability Cardiovascular Hospitalization Direction China

Effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2024年第2期9卷 265-272页

作者： Bo wu Zhenkun Xu Jie Cao Qi Wang Jun mei Jing Xie College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China National Experimental Teaching Demonstration Center for Food Science and Engineering Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquatic Product Processing and Preservation Shanghai201306China Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation Shanghai201306China

Transport in water is the most common method for transporting live fish in China,however,transport is a strong *** stress could lead to a reduced immune and antioxidant system function of tiger grouper,resulting in sickness and ***,tiger grouper were continuously stressed during transport,which resulted in quality *** is necessary that find a way to relieve the stress of transportation of tiger *** acid is not only a good anti-stress agent,but it is also an effective immunostimulant.β-1,3-glucan is a feed additive that can enhance the immune response of ***,this study evaluated the effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated *** indicated that addingβ-1,3-glucan and ascorbic acid in transport-water muted the increase of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)*** addition,β-1,3-glucan and ascorbic acid activated Nrf2 and mediated TOR expression and then up-regulate related mRNA expression of antioxidant and immune *** concluded that the application ofβ-1,3-glucan and ascorbic acid inhibit the increase of metabolism enzymes and inflammatory factors and activate immune and antioxidant signaling pathways to relieve oxidant stress,immune response,and *** the loss of amino acids provided nutrients to relieve oxidative stress and immune response,which demonstrated immune-nutritional response in live tiger grouper during simulated *** results may provide a new solution for alleviating the decline of immune and antioxidant function of tiger grouper caused by transportation stress.

关键词： Tiger grouper β-1,3-Glucan Ascorbic acid Immune response Antioxidant signaling pathway

Adiposity in Chinese people with type 1 diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第7期15卷 1404-1408页

作者： Nian-Wei wu Xia-Fei Lyu Zhen-mei An She-Yu Li Department of Endocrinology and Metabolism West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Radiology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Guideline and Rapid Recommendation Chinese Evidence-based Medicine CenterCochrane China Center and MAGIC China CenterWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

Adiposity,synonymous with obesity,is prevalent among both children and adults with type 1 diabetes in *** literature underscored the pathophysiological and socioeconomic factors associated with adiposity,and consistently highlighted its impact on cardiovascular,kidney,and metabolic diseases among Chinese individuals with type 1 *** and managing adiposity in individuals with type 1 diabetes are complicated and entail comprehensive approaches including lifestyle modifications,cognitive-behavioral therapy,insulin dose titration,and other diabetes treatment *** condition calls for coordination among policymakers,researchers,clinicians,and patients.

关键词： Type 1 diabetes China Metabolic syndrome Obesity Anti-obesity medications