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Epigenetic silencing schlafen-11 sensitizes esophageal cancer to ATM inhibitor

World Journal of Gastrointestinal Oncology 2024年第5期16卷 2060-2073页

作者： Jing Zhou mei-ying zhang Ai-Ai Gao Cheng Zhu Tao He James G Herman Ming-Zhou Guo School of Medicine NanKai UniversityTianjin 300071China Department of Gastroenterology and Hepatology The First Medical CenterChinese PLA General HospitalBeijing 100853China Departments of Pathology Characteristic Medical Center of The Chinese People’s Armed Police ForceTianjin 300162China The Hillman Cancer Center University of Pittsburgh Cancer InstitutePittsburghPA 15213United States National Key Laboratory of Kidney Diseases Chinese PLA General HospitalBeijing 100853China

BACKGROUND Targeting DNA damage response(DDR)pathway is a cutting-edge *** has been reported that Schlafen-11(SLFN11)contributes to increase chemosensitivity by participating in ***,the detailed mechanism is *** To investigate the role of SLFN11 in DDR and the application of synthetic lethal in esophageal cancer with SLFN11 *** To reach the purpose,eight esophageal squamous carcinoma cell lines,142 esophageal dysplasia(ED)and 1007 primary esophageal squamous cell carcinoma(ESCC)samples and various techniques were utilized,including methylationspecific polymerase chain reaction,CRISPR/Cas9 technique,Western blot,colony formation assay,and xenograft mouse *** Methylation of SLFN11 was exhibited in 9.15%of(13/142)ED and 25.62%of primary(258/1007)ESCC cases,and its expression was regulated by promoter region ***11 methylation was significantly associated with tumor differentiation and tumor size(both P<0.05).However,no significant associations were observed between promoter region methylation and age,gender,smoking,alcohol consumption,TNM stage,or lymph node *** DNA damaged model induced by low dose cisplatin,SLFN11 was found to activate non-homologous end-joining and ATR/CHK1 signaling pathways,while inhibiting the ATM/CHK2 signaling *** silencing of SLFN11 was found to sensitize the ESCC cells to ATM inhibitor(AZD0156),both in vitro and in *** SLFN11 is frequently methylated in human *** of SLFN11 is sensitive marker of ATM inhibitor in ESCC.

关键词： Schlafen-11 Esophageal squamous cell carcinoma DNA methylation Synthetic lethality AZD0156

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Aβ28-Induced Specific and Effective Serum Antibodies Against Aβ42

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Chemical Research in Chinese Universities 2010年第6期26卷 974-980页

作者： WANG Jia-peng CUI Li-li HUANG Xue-mei zhang ying-jiu Key Laboratory for Molecular Enzymology ＆ Engineering Ministry of Education Jilin University Changchun 130021 P. R. China

Though phase I clinical trial of immunotherapy for Alzheimer＇s disease（AD） with inoculated Aβ42 in humans had been proven to be effective,phase II immunotherapy trial was discontinued in 2002 because a few patients developed significant inflammatory reactions caused by C-terminal domain of Aβ*** aim of the study was to investigate the levels and the abilities of antibodies induced by C-terminal truncated Aβ species to inhibit Aβ42 aggregation and cytotoxity in vitro.46-week-old male BALB/c mice,Aβ42 and Aβ28/Aβ35/Aβ42 with a C-terminal eight-histidine tag（Aβ28H8,Aβ35H8,and Aβ42H8） were applied in the present *** mice were randomly divided into 5 groups（n=8）,control mice immunized with the normal saline,Aβ42-immunized mice and Aβ42H-/Aβ35H-/Aβ28H-immunized *** the serum antibodies were evaluated by measuring their abilities to inhibit Aβ42 aggregation and cytotoxity in *** mouse was vaccinated with purified Aβ peptide emulsified with Freund＇s adjuvant（volume ratio 1：1）.Titers,concentrations and isotypes of serum antibodies against Aβ42 were measured by indirect *** of serum antibodies on Aβ42 aggregation and disassembly of Aβ42 fibrils in vitro were observed by electron *** of serum antibodies on Aβ42 cytotoxicity were determined by MTT assay.Aβ42 or Aβ28 could induce higher anti-Aβ42 antibody titer（1：6400） than Aβ35（1：3200）.Significantly,Aβ28 induced more IgG1 and IgG2b isotype antibodies and less IgG2b isotype antibody than other Aβ species though all the induced serum antibodies could inhibit Aβ42 aggregation or fibrillogenesis,could induce the disassembly of Aβ42 aggregates,and neutralized or inhibited the cytotoxicity of Aβ42 in vitro.C-Terminal truncated Aβ28 could induce the same effective but safer serum antibodies against Aβ42 than full length Aβ42 by eliciting more Th2-type immune responses.

关键词： Alzheimer's disease Amyloid peptide Antibody Epitope Immunization

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First total synthesis of paecilodepsipeptide A

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Chinese Chemical Letters 2009年第5期20卷 527-530页

作者： Ming Jun Yang Jing Wu Zhong Duo Yang ying mei zhang School of Life Sciences Lanzhou University Lanzhou 730000 China School of Life Science and Engineering Lanzhou University of Technology Lanzhou 730050 China

The first total synthesis of paecilodepsipeptide A is reported. A convergent, flexible strategy employing peptide chemistry and culminating in macrolactamisation is described. The previously reported structure of comp... 详细信息

关键词： Paecilodepsipeptide A Macrolactamisation Total synthesis

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Expression Characterization and Preparation of Human Amyloid Precursor Protein in Escherichia coli

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Chemical Research in Chinese Universities 2009年第1期25卷 64-68页

作者： XU Guang-wei WANG Jia-peng HUANG Xue-mei zhang ying-jiu Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education Jilin University Changehun 130021 P R. China

To analyze whether expressed amyloid precursor protein（APP） existed in hydrophilic（cytoplasmid） or hydrophobic（lipid bilayer） environment in E. coli and to obtain intact APP for study on its function, we investigated the expression characterization and preparation of the three intact isoforms APP770, APP751, and APP695 in E. coll. The results show that these expressed APPs existed both in hydrophilic cytoplasm region as inclusion bodies and hydrophobic membrane region as membrane-bound state in E. coll. APPs in inclusion bodies were purified on an NTA-Ni^2＋ agarose column after dissolving in the urea buffer and APPs in membrane-bound state were obtained by ultracentrifugation. The activity analysis indicates that APP770 and APP751 exhibited strong trypsin-inhibitory activity like the natural ones. These results indicate that E. coli cells can be used as host cells for the expression of human integral membrane protein like APP in either soluble or membrane-bound state unless the interest protein undergone post-translational modification is required.

关键词： Amyloid precursor protein Expression characterization Preparation Membrane Inclusion body

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Compensation temperatures and hysteresis behaviors of a graphene-like trilayer

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Communications in Theoretical Physics 2024年第5期76卷 166-179页

作者： ying An Ji Liang Wei Wang mei-mei Du Feng-ge zhang School of Science Shenyang University of TechnologyShenyang 110870China School of Electrical Engineering Shenyang University of TechnologyShenyang 110870China

This work focuses on the ground-state phase diagram,the compensation temperatures and the critical behaviors of a ferrimagnetic graphene-like trilayer induced by crystal fields and exchange *** simulation results show that a negative decrease in crystal field or an increase in exchange coupling can increase the critical *** importantly,an M curve with double compensation temperatures can be observed,which is not predicted by the Neel *** remarkable compensation phenomenon has potential application value in the field of magnetic recording.

关键词： graphene-like trilayer mixed-spin Ising model magnetization double compensation temperatures triple-loop hysteresis monte Carlo

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Research on intelligent search-and-secure technology in accelerator hazardous areas based on machine vision

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Nuclear Science and Techniques 2024年第4期35卷 96-107页

作者： ying-Lin Ma Yao Wang Hong-mei Shi Hui-Jie zhang School of Traffic and Transportation Beijing Jiaotong UniversityBeijing 100044China School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Prompt radiation emitted during accelerator operation poses a significant health risk,necessitating a thorough search and securing of hazardous areas prior to ***,manual sweep methods are ***,the limitations of manual sweeps have become increasingly evident with the implementation of large-scale *** leveraging advancements in machine vision technology,the automatic identification of stranded personnel in controlled areas through camera imagery presents a viable solution for efficient search and *** the criticality of personal safety for stranded individuals,search and security processes must be sufficiently *** ensure comprehensive coverage,180°camera groups were strategically positioned on both sides of the accelerator tunnel to eliminate blind spots within the monitoring *** YOLOV8 network model was modified to enable the detection of small targets,such as hands and feet,as well as larger targets formed by individuals near the ***,the system incorporates a pedestrian recognition model that detects human body parts,and an information fusion strategy is used to integrate the detected head,hands,and feet with the identified pedestrians as a cohesive *** strategy enhanced the capability of the model to identify pedestrians obstructed by equipment,resulting in a notable improvement in the recall ***,recall rates of 0.915 and 0.82were obtained for Datasets 1 and 2,*** there was a slight decrease in accuracy,it aligned with the intended purpose of the search-and-secure software *** tests conducted within an accelerator tunnel demonstrated the effectiveness of this approach in achieving reliable recognition outcomes.

关键词： Search and secure Machine vision Camera Human body parts recognition Particle accelerator Hazardous area

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Efficacy of different antibiotics in treatment of children with respiratory mycoplasma infection

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World Journal of Clinical Cases 2021年第23期9卷 6717-6724页

作者： mei-ying zhang Yan Zhao Jin-Feng Liu Guo-Ping Liu Rui-Yun zhang Li-Min Wang Department of Pediatrics Qingdao Municipal HospitalQingdao 266011Shandong ProvinceChina Department of ICU Jinan City People’s HospitalJinan 271199Shandong ProvinceChina Department of Interventional Radiology Affiliated Hospital of Qingdao UniversityQingdao 266003Shandong ProvinceChina

BACKGROUND Respiratory infections in children are common pediatric diseases caused by pathogens that invade the respiratory *** are considerably susceptible to Mycoplasma pneumoniae *** has been widespread clinical attention on treatment strategies for this *** To analyze the clinical efficacy of different antibiotics in treating pediatric respiratory mycoplasma *** We included 106 children with a confirmed diagnosis of respiratory mycoplasma infection who were admitted to our hospital from April 2017 to July 2019 and grouped them using a random number *** them,53 children each received clarithromycin or *** clinical efficacy of both drugs was evaluated and *** performed the multiplex polymerase chain reaction(MP-PCR)test and determined the MP-PCR negative rate in children after the end of the treatment *** compared the incidence of toxic and side effects,including nausea,diarrhea,and abdominal pain;further,we recorded the length of hospitalization,antipyretic time,and drug ***,we evaluated and compared the compliance of the children during *** The erythromycin group showed a significantly higher total effective rate of clinical treatment than the clarithromycin ***-PCR test results showed that the clarithromycin group had a significantly higher MP-PCR negative rate than the erythromycin ***,children in the clarithromycin group had shorter fever time,shorter hospital stays,and lower drug costs than those in the erythromycin *** clarithromycin group had a significantly higher overall drug adherence rate than the erythromycin *** incidence of toxic and side effects was significantly lower in the clarithromycin group than in the erythromycin group(P<0.05).CONCLUSION Our findings indicate that clarithromycin has various advantages over erythromycin,including higher application safety,stronger mycoplasma clearance,and higher medicatio

关键词： Clarithromycin Erythromycin Mycoplasma respiratory infection Children Clinical efficacy Drug compliance Toxic side effects

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Reinforcement of High Impact Polystyrene by Aramid Nanoparticle Fillers Prepared via an In situ Bottom-up Approach

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Chinese Journal of Polymer Science 2024年第8期42卷 1167-1176页

作者： Xiang-Jun Gong Zhi-Ping Zhao Chang-mei Sun ying zhang Rong-Jun Qu ying Wang School of Chemistry and Materials Science Ludong UniversityYantai 264025China

In this work,aramid nanoparticles(ANPs)were prepared in dimethyl formamide(DMF)solution containing high impact polystyrene(HIPS)via a bottom-up *** electron microscopy(TEM)images showed that the obtained ANPs were evenly distributed in the HIPS matrix without any *** composition of the ANPs,i.e.,poly(p-phenyl-p-phenylenediamine)(PPTA),was confirmed by Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS)and X-ray diffractometer(XRD).The ANP/HIPS composites,obtained after ethanol precipitation,were added to neat HIPS as fillers to fabricate ANP/HIPS composite *** surface roughness and the glass transition temperature(T_g)of the sheets were characterized by atomic force microscope(AFM)and differential scanning calorimetry(DSC),*** with neat HIPS,the mechanical properties of the composite sheet were significantly improved,and the Young's modulus increased from 246.55 MPa to 2025.12 MPa,the tensile strength increased from 3.07 MPa to 29.76 MPa,and the toughness increased from 0.32 N/mm^2 to 3.92 N/mm^2,with an increase rate of 721%,869%and 1125%,***,the thermal stability of the composites improved with the increase in ANP content.

关键词： High impact polystyrene PPTA nanoparticle Reinforce Mechanical property

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Possibility of reaching the predicted center of the“island of stability”via the radioactive beam-induced fusion reactions

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Nuclear Science and Techniques 2024年第9期35卷 186-195页

作者： Ming-Hao zhang ying Zou mei-Chen Wang Gen zhang Qing-Lin Niu Feng-Shou zhang The Key Laboratory of Beam Technology of Ministry of Education School of Physics and AstronomyBeijing Normal UniversityBeijing 100875China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China School of Physical Science and Technology Guangxi UniversityNanning 530004China Center of Theoretical Nuclear Physics National Laboratory of Heavy Ion Accelerator of LanzhouLanzhou 730000China

Based on the dinuclear system model,the synthesis of the predicted double-magic nuclei^(298)Fl and 304120 was investigated via neutron-rich radioactive beam-induced fusion *** reaction^(58)Ca+^(244)Pu is predicted to be favorable for producing^(298)Fl with a maximal ER cross section of 0.301 *** of the entrance channel effect reveal that the^(244)Pu target is more promising for synthesizing^(298)Fl than the neutron-rich targets^(248)Cm and^(249)Bk,because of the influence of the Coulomb *** the synthesis of 304120,the maximal ER cross section of 0.046 fb emerges in the reaction^(58)V+^(249)Bk,indicating the need for further advancements in both experimental facilities and reaction mechanisms.

关键词： Superheavy nuclei Dinuclear system model Fusion reaction Double-magic nucleus Radioactive beam

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Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage

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Neural Regeneration Research 2023年第6期18卷 1308-1315页

作者： Zi-Xian Zhou Qi Cui ying-mei zhang Jia-Xin Yang Wen-Jing Xiang Ning Tian Yan-Lin Jiang mei-Ling Chen Bin Yang Qing-Hua Li Ru-Jia Liao Laboratory of Neuroscience Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Department of Neurology Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Guangxi Clinical Research Center for Neurological Diseases Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina Department of Pharmacology Affiliated Hospital of Guilin Medical UniversityGuilin Medical UniversityGuilinGuangxi Zhuang Autonomous RegionChina

Recent studies have indicated that suppressing oxidative stress and ferroptosis can considerably improve the prognosis of intracerebral hemorrhage(ICH).Withaferin A(WFA),a natural compound,exhibits a positive effect on a number of neurological ***,the effects of WFA on oxidative stress and ferroptosis-mediated signaling pathways to ICH remain *** this study,we investigated the neuroprotective effects and underlying mechanism for WFA in the regulation of ICH-induced oxidative stress and *** established a mouse model of ICH by injection of autologous tail artery blood into the caudate nucleus and an in vitro cell model of hemin-induced *** was injected intracerebroventricularly at 0.1,1 or 5μg/kg once daily for 7 days,starting immediately after ICH *** markedly reduced brain tissue injury and iron deposition and improved neurological function in a dose-dependent manner 7 days after cerebral *** in vitro experiments,cell viability test showed that WFA protected SH-SY5Y neuronal cells against hemin-induced cell ***-linked immunosorbent assays in vitro and in vivo showed that WFA markedly decreased the level of malondialdehyde,an oxidative stress marker,and increased the activities of anti-oxidative stress markers superoxide dismutase and glutathione peroxidase after *** blot assay,quantitative polymerase chain reaction and immunofluorescence results demonstrated that WFA activated the nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)signaling axis,promoted translocation of Nrf2 from the cytoplasm to nucleus,and increased HO-1 *** Nrf2 with siRNA completely reversed HO-1 expression,oxidative stress and protective effects of ***,WFA reduced hemin-induced ***,after treatment with an HO-1 inhibitor,the neuroprotective effects of WFA against hemin-induced ferroptosis were *** test results showed that WFA combined with ferrostatin-1

关键词： behavior brain injuries hemorrhagic stroke ferroptosis heme oxygenase-1 neuroprotection nuclear factor E2-related factor 2 nuclear translocator oxidative stress stroke

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