检索结果-南通市图书馆

一例临床药师参与卵巢癌患者使用帕博利珠单抗致免疫相关性肺炎和心肌炎的病例分析

在线全文

学校读者我要写书评

暂无评论

中国处方药 2024年第7期22卷 126-129页

作者：陈春梅崔朴梅吴吉进昆明理工大学附属安宁市第一人民医院药学部昆明650300 昆明理工大学附属安宁市第一人民医院肿瘤科昆明650300

目的分析1例帕博利珠单抗致免疫相关性肺炎和心肌炎的临床特点、发生机制及诊治方案。方法临床药师检索相关文献,总结帕博利珠单抗治疗卵巢癌癌诱发免疫相关性肺炎和心肌炎的临床特点、发生机制,并协助诊治。结果经积极治疗后,患者转归... 详细信息

目的分析1例帕博利珠单抗致免疫相关性肺炎和心肌炎的临床特点、发生机制及诊治方案。方法临床药师检索相关文献,总结帕博利珠单抗治疗卵巢癌癌诱发免疫相关性肺炎和心肌炎的临床特点、发生机制,并协助诊治。结果经积极治疗后,患者转归较好。结论临床药师参考免疫毒性管理指南对不良反应进行分级,给出治疗建议,转归较好,体现了临床药师的服务价值。

关键词：铂耐药卵巢癌免疫相关性肺炎免疫相关性心肌炎病例分析

Effects of Non-specific and Specific Solvation on Adsorption of BPTI on Au Surface： Insight from Molecular Dynamics Simulation

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Physics 2013年第5期26卷 558-568,I0003,I0004页

作者： Wei Yang Li-yun Zhang Meng-long Li Xue-mei pu Nan-rong Zhao Faculty of Chemistry Sichuan University Chengdu 610064 China

Proteins adsorption at solid surfaces are of paramount important for many natural processes. However, the role of specific water in influencing the adsorption process has not been well understood. We used molecular dynamics simulation to study the adsorption of BPTI on Au surface in three water environments （dielectric constant model, partial and full solvation models）. The result shows that a fast and strong adsorption can occur in the dielectric environment, which leads to significant structure changes, as confirmed by great deviation from the crystal structure, largely spreading along the Au surface, rapid lose in all secondary structures and the great number of atoms in contact with the surface. Compared to the dielectric model, slower adsorption and fewer changes in the calculated properties above are observed in the partial solvation system since the specific water layer weakens the adsorption effects. However, in the partial solvation system, the adsorption of polar Au surface causes a significant decrease in the specific hydration around the protein, which still results in large structure changes similar to the dielectric system, but with much less adsorption extent. Enough water molecules in the full solvation system could allow the protein to rotate, and to large extent preserve the protein native structure, thus leading to the slowest and weakest adsorption. On the whole, the effects of non-specific and specific solvation on the protein structure and adsorption dynamics are significantly different, highlighting the importance of the specific water molecule in the protein adsorption.

关键词： Adsorption Au surface Implicit water Partial solvation Full solvation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

枸杞叶片酚酸类物质的自毒作用分析

江苏农业学报 2024年第2期40卷 213-222页

作者：宋燕芳彭彤马少兰马彩霞高娜李凯乐张传继蒲美运纳小凡兰州大学生命科学学院甘肃兰州730000 细胞活动与逆境适应教育部重点实验室甘肃兰州730000 彭阳县第一中学宁夏固原756500 宁夏大学生命科学学院宁夏银川750021

自毒作用是植物连作障碍形成的主要原因之一,严重影响植物生长和粮食生产。前期研究结果表明,土壤微生物多样性下降、土传病害加重是枸杞园连作障碍形成的重要原因,但枸杞残体的自毒作用及其物质基础目前仍不明确。为探讨自毒物质的主... 详细信息

自毒作用是植物连作障碍形成的主要原因之一,严重影响植物生长和粮食生产。前期研究结果表明,土壤微生物多样性下降、土传病害加重是枸杞园连作障碍形成的重要原因,但枸杞残体的自毒作用及其物质基础目前仍不明确。为探讨自毒物质的主要来源,本研究对比分析了枸杞(Lycium barbarum L.)叶、茎、根等组织浸提液对枸杞幼苗生长的影响。浓度梯度试验结果表明,叶片浸提液的抑制效应高于根、老根皮及茎浸提液。向无枸杞种植历史的农田土壤中添加枸杞叶片干粉能够显著抑制枸杞幼苗生长及叶片光合作用,证实了枸杞叶片具有自毒作用。联合UPLC-QTOF-MS和GC-TOF-MS技术对生长季末期枸杞叶片进行代谢组分析,共鉴定出有机酸112种、醇类41种、氨基酸37种、醛酮类28种和糖类48种。对其中24种有机酸及衍生物的分析发现,20种有机物能够不同程度抑制枸杞幼苗初生根生长。其中水杨酸、邻苯二甲酸、对羟基苯甲酸和香豆素在浓度低至10μmol/L时仍具显著抑制效应。UPLC结果进一步显示,长期连作导致枸杞园土壤香豆素、水杨酸、苯甲酸、阿魏酸和香豆酸积累。此外,土壤总酚酸含量也随种植年限增加显著增加。这些结果表明土壤中枸杞叶片酚酸能够在长期连作下积累并诱发自毒作用,这为进一步探索枸杞连作障碍形成机制提供了研究基础。

关键词：枸杞连作障碍自毒物质叶片酚酸

维普期刊数据库博看期刊

Fourier-domain optical coherence tomography-guided phototherapeutic keratectomy for the treatment of anterior corneal scarring

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2020年第11期13卷 1720-1726页

作者： Yu-Li Yang Qian Jian Bo Liu Ke Wang Yu-Juan Chen Lian Tan mei-Jun pu Yong Liu Southwest Hospital/Southwest Eye Hospital Third Military Medical University(Anny Medical University)Chongqing 400038China Chongqing Aier Mega Eye Hospital Aier Eye Hospital GroupChongqing 400060China

AIM:To evaluate the safety,visual and anatomic outcomes of fourier-domain optical coherence tomography(FD-OCT)-guided excimer laser phototherapeutic keratectomy(PTK)combined with photorefractive keratectomy(PRK)surgery in treating anterior corneal ***:Clinical data of 23 eyes of 21 patients with anterior corneal scarring underwent FD-OCT-guided PTK and PRK from Dec.2014 to Jul.2016 were *** were assessed for preoperative and postoperative uncorrected visual acuity(UCVA),best spectacle-corrected visual acuity(BSCVA),contrast sensitivity(CS),FD-OCT,corneal topography and colour figures of anterior ***:The preoperative corneal pathologic conditions included viral keratitis(7 patients,7 eyes),band keratopathy(2 patients,4 eyes),corneal dystrophy(4 patients,4 eyes),traumatic corneal disease(2 patients,2 eyes)and corneal chemical injury(6 patients,6 eyes).Mean follow-up time was 10.65(range,3-19)***(in IogMAR)improved from a mean of 0.79(95%Cl,0.281.29)preoperatively to a mean of 0.45(95%Cl,0.29-0.62)postoperatively(P=0.021).BSCVA(in IogMAR)improved from 0.57(95%Cl,0.27-0.88)preoperatively to a mean of 0.28(95%Cl,0.15-0.41)postoperatively(P=0.001).Corneal topographic indices postoperatively showed significant improvement in corneal cylinder(P=0.009),the surface regularity index(P=0.007)and surface asymmetry index(P=0.00).Postoperative spherical equivalent averaged-0.53 diopters(-1.49 to 0.42).No complications were associated with the ***:FD-OCT-guided PTK combined with PRK is safe and effective for the treatment of anterior corneal scarring by eliminating or reducing corneal opacities.

关键词： corneal scar phototherapeutic keratectomy fourier-domain optical coherence tomography corneal opacity corneal topographic indices

Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2020年第8期62卷 1213-1226页

作者： Shi-Xin Zhou Yong Zhu Liang-Fang Wang Ya-Ping Zheng Jin-Feng Chen Ting-Ting Li Xue-mei Yang He Wang Xu-pu Li Xiao-Chun Ma Ji-Qun Zhao mei pu Hui Feng Yan Li Jing Fan Ji-Wei Zhang Yan-Yan Huang Wen-Ming Wang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Rice Research Institute and Key Lab for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China

MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced ***-miR1873 is a rice-specific miRNA targeting LOC_***,we show that Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_***-miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post-inoculation(hpi)of *** Osa-miR1873 enhanced susceptibility to *** and compromised induction of defense *** contrast,blocking Osa-miR1873 through target mimicry compromised susceptibility to *** and enhanced induction of defense *** expression of Osa-miR1873 also resulted in some defects in yield traits,including grain numbers and seed setting ***,overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and *** together,we demonstrate that Osa-miR1873 fine-tunes the rice immunity-growth trade-off via LOC_Os05g01790,and blocking Osa-miR1873 could improve blast disease resistance without significant yield ***,the Osa-miR1873-LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance.

关键词： miR187 immunity traits

Osa-miR167d facilitates infection of Magnaporthe oryzae in rice

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2020年第5期62卷 702-715页

作者： Zhi-Xue Zhao Qin Feng Xiao-Long Cao Yong Zhu He Wang Viswanathan Chandran Jing Fan Ji-Qun Zhao mei pu Yan Li Wen-Ming Wang Rice Research Institute and Key Lab for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China Department of Biotechnology Sree Narayana Guru CollegeCoimbatoreTamilnaduIndia

MicroRNAs(miRNAs)play important roles in rice response to Magnaporthe oryzae,the causative agent of rice blast *** the roles of rice miRNAs is of great significance for the disease ***-miR167d belongs to a conserved miRNA family targeting auxin responsive factor(ARF)genes that act in developmental and stress-induced ***,we show that OsamiR167d plays a negative role in rice immunity against *** by suppressing its target *** expression of Osa-miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation(hpi),however,its expression was significantly increased at 24 hpi in the susceptible accession upon *** *** rice lines over-expressing Osa-miR167d were highly susceptible to multiple blast fungal *** contrast,transgenic lines expressing a target mimicry to block OsamiR167d enhanced resistance to rice blast *** addition,knocking out the target gene ARF12 led to hypersusceptibility to multiple blast fungal *** together,our results indicate that Osa-miR167d negatively regulate rice immunity to facilitate the infection of *** by downregulating ***,Osa-miR167d-ARF12 regulatory module could be valuable in improvement of blast-disease resistance.

关键词： immunity expressing infection

A K-means Optimized Clustering Algorithm Based on Improved Genetic Algorithm

在线全文

学校读者我要写书评

暂无评论

A K-means Optimized Clustering Algorithm Based on Improved G...

Osa-miR439 Negatively Regulates Rice Immunity Against Magnaporthe oryzae

2021中国智能自动化大会(CIAC2021)

作者： Qiu-mei pu Qiong Wu Qian Li Minzu University of China

The K-means algorithm is highly sensitive to the initial clustering centers and easily get trapped in a local optimum. To avoid such problems, this paper proposes an improved crossover operator of chromosomes in the genetic algorithm, redefines the calculation method of genetic probability and the natural selection rules, introduces different individual selection mechanisms for the two adjacent generations of chromosomes, and integrates the K-means algorithm into the improved genetic algorithm. Experiment results demonstrate that the improved K-Means algorithm is better than the original genetic algorithm and KMeans algorithm in clustering performance, far better than the bisecting K-means algorithm.

关键词： Clustering K-means algorithm Genetic algorithm

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rice science 2021年第2期28卷 156-165页

作者： LU Junhua YANG Xuemei CHEN Jinfeng LI Tingting HU Zijin XIE Ying LI Jinlu ZHAO Jiqun pu mei FENG Hui FAN Jing HUANG Yanyan ZHANG Jiwei WANG Wenming LI Yan State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Rice Research Institute and Key Laboratory for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China

Osa-miR439 is a rice-specific microRNA family. Here we showed that Osa-miR439 acted as anegative regulator in rice immunity against blast fungus Magnaporthe oryzae. Osa-miR439 differentiallyresponded to M. oryzae between susceptible and resistant rice accessions. The accumulation ofOsa-miR439 was constitutively more in the susceptible accession than in the resistant one. Transgeniclines overexpressing Osa-miR439a (OX439a) showed higher susceptibility associating with lower inductionof defense-related genes and less hydrogen peroxide (H2O2) accumulation at the infection sites than thecontrol plants. In contrast, transgenic lines expressing a target mimic of Osa-miR439 (MIM439) displayedcompromised susceptibility associating with increased H2O2 accumulation. Furthermore, we found thatthe expression of three predicted target genes was decreased in OX439a but increased in MIM439 incomparison to control plants, and this expression was differential in susceptible and resistant accessionsupon M. oryzae infection, indicating that Osa-miR439a may regulate rice blast resistance via these *** results unveiled the role of Osa-miR439a in rice blast resistance and provided the potentiality toimprove the blast resistance via miRNA.

关键词： Osa-miR439 rice blast resistance target gene

建立在固定样地上的林分生长线性联立方程组模型研究

在线全文

学校读者我要写书评

暂无评论

北京林业大学学报 2007年第1期29卷 37-41页

作者：郎璞玫中国林业科学研究院资源信息研究所林业遥感与信息技术实验室

为描述森林资源连续清查中两期固定样地因子之间的关系,该文分析了两期林分生长变量间的关系,以固定样地的两期(1990和1994年)观测变量为资料,在Borders模型基础上,引入标准地位指数和变量的线性变换,分优势树种构造出间隔期为5年的林... 详细信息

为描述森林资源连续清查中两期固定样地因子之间的关系,该文分析了两期林分生长变量间的关系,以固定样地的两期(1990和1994年)观测变量为资料,在Borders模型基础上,引入标准地位指数和变量的线性变换,分优势树种构造出间隔期为5年的林分生长线性联立方程组模型簇YB+xΓ=ε,并获得该模型待估参数矩阵B、Γ的无偏估计.该模型是可识别且误差结构矩阵不是对角矩阵的联立方程组模型.它表述了样地期末断面积和蓄积是林分标准地位指数、年龄、期初断面积的隐函数,较深入地描述了两期固定样地林分因子相互依存、从属的关系.通过模型的外检验,蓄积平均精度可达90%以上,林分纯生长量的估计精度可达80%以上.该模型具有较好的适应性,并可在今后若干个离散的时间点上预估同类林分的收获量,为研究在固定样地上两期林分自然生长规律提供较科学的理论和方法.

关键词：林分生长标准地位指数联立方程组离散化估计