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Changes of Reactive Oxygen Species and Related Enzymes in Mitochondrial Respiration During Storage of Harvested Peach Fruits

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Agricultural Sciences in China 2011年第1期10卷 149-158页

作者： KAN Juan WANG Hong-mei jin Chang-hai College of Food Science and Engineering Yangzhou University Yangzhou 225127 P.R.China

Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ROS） and related enzymes in mitochondria respiration during storage and then their influence on senescence of harvested Peach fruits was studied. The results showed that low temperature （5℃） strongly inhibited the reduction of firmness and the increase in respiration rate. During storage at ambient temperature （20℃）, ROS had a cumulative process while malondialdehye （MDA） content continued to increase in associated with enhanced membrane lipid peroxidation. Lipoxygenase （LOX） activity was strongly inhibited under the low temperature condition. The activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, and Ca^2＋-ATPase declined to a certain extent at ambient temperature, while they showed higher activities at low temperature, which may be related to lower membrane lipid peroxidation at low temperature. Higher Ca^2＋ content at ambient temperature may be responsible for impairment of mitochondrial function, thus, leading to fruit senescence. The results showed that under low temperature condition, the low accumulation of ROS and the low level of membrane lipid peroxidation could maintain the function of mitochondria that would help to delay the senescence of peach fruits. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration. It can be inferred that the low temperature helps to delay senescence of peach fruits via suppression of ROS and related enzymes, maintain better homeostasis of Ca^2＋ in mitochondria and thus better mitochondrial functions.

关键词： peach fruits ripening active oxygen enzymes related to mitochondria respiratory metabolism

Regulation of influenza virus infection by microRNAs

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Journal of Integrative Agriculture 2019年第7期18卷 1421-1427页

作者： ZOU Zhong GONG Wen-xiao HUANG Kun SUN Xiao-mei jin mei-lin State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070P R.China College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070P.R. China Key Laboratory of Development of Veterinary Diagnostic Products Ministry of Agriculture and Rural AffairsWuhan 430070P.R. China

MicroRNAs(miRNAs) are small noncoding RNAs of 18–25 nucleotides(nt) in length that represent key regulators of many normal cellular functions through the inhibition of mRNA translation and mRNA *** date,over 2 500 mature miRNAs have been identified in plants,animals and several types of *** A virus(IAV),which is a negativesense,single-stranded RNA virus,does not encode viral ***,IAV infection can alter the expression of host miRNAs,either in cell culture or in *** turn,host miRNAs regulate IAV life cycle through directly binding to IAV genome or indirectly targeting host factors associated with viral *** this review,we briefly summarized the role and significance of miRNA in relation to IAV *** the role of cellular miRNAs during viral infection may be beneficial to the identification of novel therapeutic strategies to block IAV replication.

关键词： microRNAs influenza A virus pathogenesis therapeutic strategies

Extreme plastid RNA editing may confound phylogenetic reconstruction:A case study of Selaginella(lycophytes)

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Plant Diversity 2020年第5期42卷 356-361页

作者： Xin-Yu Du jin-mei Lu De-Zhu Li Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of Sciences132 Lanhei RoadKunmingYunnan 650201China Kunming College of Life Science University of Chinese Academy of Sciences19 Qingsong RoadKunmingYunnan 650201China

Cytidine-to-uridine(C-to-U)RNA editing is common in coding regions of organellar genomes throughout land *** most cases RNA editing alters translated amino acids or creates new start codons,potentially confounds phylogenetic *** this study,we used the spike moss genus Selaginella(lycophytes),which has the highest frequency of RNA editing,as a model to test the effects of extreme RNA editing on phylogenetic *** predicted the C-to-U RNA editing sites in coding regions of 18 Selaginella plastomes,and reconstructed the phylogenetic relationships within Selaginella based on three data set pairs consisted of plastome or RNA-edited coding sequences,first and second codon positions,and translated amino acid sequences,*** predicted between 400 and 3100 RNA editing sites of 18 Selaginella *** numbers of RNA editing sites in plastomes were highly correlated with the GC content of first and second codon positions,but not correlated with the GC content of plastomes as a *** phylogenetic analyses showed that there were substantial differences(e.g.,the placement of clade B in Selaginella)between the phylogenies generated by the plastome and RNA-edited data *** empirical study provides evidence that extreme C-to-U RNA editing in the coding regions of organellar genomes alters the sequences used for phylogenetic reconstruction,and might even confound phylogenetic ***,RNA editing sites should be corrected when plastid or mitochondrial genes are used for phylogenetic studies,particularly in those lineages with abundant organellar RNA editing sites,such as hornworts,quillworts,spike mosses,and some seed plants.

关键词： GC content Land plants Organellar genome Phylogenomics RNA editing

Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis

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Asian Journal of Andrology 2023年第3期25卷 314-321页

作者： Xiao-Li Wang jin-mei Li Shui-Qiao Yuan Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Laboratory of Animal Center Huazhong University of Science and TechnologyWuhan 430030China Shenzhen Huazhong University of Science and Technology Research Institute Shenzhen 518057China

Mammalian testis exhibits remarkably high transcriptome complexity,and spermatogenesis undergoes two periods of transcriptional *** make the RNA-binding proteins(RBPs)the utmost importance during male germ cell *** nuclear ribonucleoproteins(hnRNPs)are a large family of RBPs implicated in many steps of RNA processing;however,their roles in spermatogenesis are largely ***,we investigated the expression pattern of 12 hnRNP family members in mouse testes and found that most detected members are highly expressed in the ***,we found that most of the detected hnRNP proteins(hnRNPD,hnRNPK,hnRNPQ,hnRNPU,and hnRNPUL1)display the highest signals in the nuclei of pachytene spermatocytes,round spermatids,and Sertoli cells,whereas hnRNPE1 exclusively concentrates in the manchette of elongating *** expression of these hnRNP proteins showed both similarities and specificity,suggesting their diverse roles in spermatogenesis.

关键词： gene expression heterogeneous nuclear ribonucleoproteins mice Sertoli cells spermatogenesis

Silver(I) Oxide Mediated Regioselective Monoacylation of 2,4-Dihydroxyls in L-Rhamnopyranosides

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Chinese Chemical Letters 2006年第8期17卷 1009-1012页

作者： Qian YANG Xing mei ZHU jin Song YANG Ministry of Education Key Laboratory of Drug Targeting School of Pharmacy Sichuan University Chengdu 610041

A series of acyls （Ac, Bz and Ts） were introduced regioselectively to the 2-hydroxyl in methyl and ally 3-O-benzyl-α-L-rhamnopyranosides mediated by silver（I） oxide in the presence of a catalytic amount of potass... 详细信息

关键词： Silver(Ⅰ) oxide acylation regioselectivity,α-L-rhamnopyranoside.

Effects of 17beta-estradiol on cell migration in male chicks distribution of primordial germ

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Asian Journal of Andrology 2008年第2期10卷 243-248页

作者： Xiu-mei jin Yi-Xiang Zhang Zan-Dong Li College of Biological Sciences China Agricultural University Beijing 100094 China School of Life Sciences Huzhou Teachers College Huzhou 313000 China

Aim： To assess whether exogenous estradiol has any effect on migration of primordial germ cells （PGCs） in the chick. Methods： Fertilized eggs were treated with 17beta-estradiol （E2）（80 lag/egg） at stage X （day 0 of incubation）, stages 8-10 （incubation 30 h） and 13-15 （incubation 55 h）. Controls received vehicle （emulsion） only. Changes in PGC number were measured on different days according to developmental stages. Results： In male right gonads, but not in female left gonads, at stages 28-30 （incubation 132 h） significant decreases in the mean number of PGCs aggregating were observed compared with the controls （P 〈 0.05） while the total PGC number in the right and left gonads at each stage did not change （P 〉 0.05）. Conclusion： The present study provides evidence that E2 has significant effects on the localization of PGCs in male right, but not female left, gonads of chicken embryos at stages 28-30, compared with controls. （Asian J Andro12008 Mar; 10： 243-248）

关键词： 17beta-estradiol primordial germ cells male chick

CALCULATION OF FLOW FIELD AND ANALYSIS OF SPAWNING SITES FOR CHINESE STURGEON IN THE DOWNSTREAM OF GEZHOUBA DAM

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Journal of Hydrodynamics 2007年第1期19卷 78-83页

作者： FU Xiao-li LI Da-mei jin Guo-yu School of Water Resource and Hydropower Wuhan University Wuhan 430072 China

The Chinese sturgeon （Acipenser Sinensis） is one of the unique and important fishery resources in China. Since the construction of the Gezhouba Dam, the traditional migration route of the sturgeon has been blocked; consequently, the length of natural spawning sites is reduced from 800 km in the past to less than 5 km at present. As an endangered species, the Chinese sturgeon has become one of the most conserved aquatic species. In this article, the flow field of its spawning states in the downstream of Gezhouba Dam was simulated and analyzed using N-S equations and k-ε turbulence model. Volume Of Fluid （VOF） method with the Finite Volume Method （FVM） was used to simulate the water-air two-phase flow to examine the computed area. On the basis of the ecological-hydraulic characteristics of Chinese sturgeon, the features of the flow field were investigated to provide theoretical support for the proper management of the Three Gorges Reservoir.

关键词： Chinese sturgeon (Acipenser Sinensis) FiniteVolume Method (FVM) Volume Of Fluid (VOF) naturalspawning site three-dimensional river channel

Synthesis of CdTe/SiO_2 Composites and Their Fluorescence Properties

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Chemical Research in Chinese Universities 2011年第4期27卷 540-542页

作者： WANG mei-ping LIN jin-hui SHAN Chuan-sheng College of Materials and Chemistry ＆ Chemical Engineering Chengdu University of Technology Chengdu 610059 P. R. China

Thioglycolic acid（TGA）-stabilized CdTe nanocrystals（NCs） were prepared with sodium tellurite as tellurium source,which avoids the cumbersome processes associated with H2Te or NaHTe *** CdTe/SiO2 composites were synthesized by a sol-gel method without the exchange of surface *** phase structure of CdTe NCs was investigated by X-ray *** comparison,some characterizations were done for both the CdTe NCs and the *** NCs and CdTe/SiO2 composites were characterized with TEM,digital camera and fluorescence *** stability of CdTe NCs and the composites were investigated in phosphate-buffered saline（PBS） buffer and the fluorescent properties of the composites were discussed in detail.

关键词： CdTe nanocrystal Sodium tellurite CdTe/SiO2

A Triphenylphosphonium Functionalized AIE Conjugated Macrocyclic Tetramal&mide for Mitochondrial-targeting Bioimaging

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Chinese Journal of Chemistry 2022年第1期40卷 39-45页

作者： Wei Zeng Mao-Hua Lin Ling-Yun Zhu mei-jin Lin Key Laboratory of Molecule Synthesis and Function Discovery College of ChemistryFuzhou UniversityFuzhouFujian 350116China College of Materials Science and Engineering Fuzhou UniversityFuzhouFujian 350116China

Developme nt of subcellular orga nelle-targeted bioimagi ng probes is of great imports nee in the field of early detection of diseases and exploring the behaviors of subcellular ***,we present a triphenyIphosphonium functionalized conjugated macrocyclic tetramaleimide(TPP-CMT)as a mitochondrial-targeting bioimaging *** core of TPP-CMT is obtained by connecting two pyrenes and two benzenes through four *** unique structure endows TPP-CMT with exceptional far red/near-i nfirared aggregati on-induced emissi on(FR/NIR-AIE)***-CMT is an excelle nt fluoresce nt emitter in most solve nts and eve n in solid *** qua ntum yield of TPP-CMT was higher tha n 27% when dissolved in comm on middle-polarity orga nic solve nt and this value remained at 28.2% in its solid *** in troducti on of four triphe ny Iphosph onium on maleimide positions endows TPP-CMT with mitochondrial-targeting *** to the FR/NIR-AIE characteristics and the mitochondri-al-targeti ng ability,this well-desig ned fluoresce nt probe was successfully employed for mitochondrial targeti ng bioimaging of HeLa and HepG2 cells.

关键词： Fluorescent probes Macrocycles Photophysics FR/NIR-AIE Mitochondrial-targeting bioimaging