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Regulation of U6 Promoter Activity by Transcriptional Interference in Viral Vector-Based RNAi

Genomics, Proteomics & Bioinformatics 2010年第3期8卷 170-179页

作者： Linghu Nie meghna das thakur Yumei Wang Qin Su Yongliang Zhao Yunfeng Feng Key Laboratory of Genome Sciences and Information Cancer Biology and Genetics Group Beijing Institute of Genomics Chinese Acadamy of Sciences Beijing 100029 China Departments of Medicine and Cell Biology Washington University St. Louis MO 63110 USA Immunocore Limited Abingdon Oxon OX14 4RX UK.

The direct negative impact of the transcriptional activity of one component on the second one in c/s is referred to as transcriptional interference （TI）. U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA （shRNA） expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector. Determining if U6 promoter activity can be affected by TI is critical for the expression of target shRNA in gene therapy or loss-of-function studies. In this research, we designed and implemented a modified retroviral system where shRNA and exogenous gene expressions were driven by two independent transcriptional units. We arranged U6 promoter driving .shRNA expression and UbiC promoter in two promoter arrangements. In primary macrophages, we found U6 promoter activity was inhibited by UbiC promoter when in the divergent arrangement but not in tandem. In contrast, PKG promoter had no such negative impact. Instead of enhancing U6 promoter activity, CMV enhancer had significant negative impact on U6 promoter activity in the presence of UbiC promoter. Our results indicate that U6 promoter activity can be affected by TI in a proximal promoter-specific and arrange- ment-dependent manner.

关键词： transcriptional interference U6 promoter shRNA retrovirus

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A Multifunctional Lentiviral-Based Gene Knockdown with Concurrent Rescue that Controls for Off-Target Effects of RNAi

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Genomics, Proteomics & Bioinformatics 2010年第4期8卷 238-245页

作者： Yunfeng Feng Linghu Nie meghna das thakur Qin Su Zhenfen Chi Yongliang Zhao Gregory D. Longmore Departments of Medicine and Cell Biology Washington University St. Louis MO 63110 USA Key Laboratory of Genome Sciences and Information Cancer Biology and Genetics Group Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China Immunocore Limited Abingdon Oxon OX14 4RX UK

The efficient, stable delivery of siRNA into cells, and the appropriate controls for non-specific off-target effects of siRNA are major limitations to functional studies using siRNA technology. To overcome these drawbacks, we have developed a single lentiviral vector that can concurrently deplete endogenous gene expression while expressing an epitope-tagged siRNA-resistant target gene in the same cell. To demonstrate the functional utility of this system, we performed RNAi-depleted α-actinin-1 （α-ACTN1） expression in human T cells. α-ACTN1 RNAi resulted in inhibited chemotaxis to SDF-1α, but it can be completely rescued by concurrent expression of RNAi-resistant α-ACTN1 （rr-α-ACTN1） in the same cell. The presence of a GFP tag on rr-α-ACTN1 allowed for detection of appropriate subcellular localization of rr-α-ACTN1. This system provides not only an internal control for RNAi off-target effects, but also the potential tool for rapid structure-function analyses and gene therapy.

关键词： lentivirus RNAi shRNA α-actinin-1 chemotaxis

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miR-153 as biomarker for cancer-functional role as tumor suppressor

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BIOCELL 2022年第1期46卷 13-26页

作者： SALONI thakur ADESH K.SAINI JOYDEEP das VIPIN SAINI PARIN BALHARA JAGPREET S.NANDA REENA V.SAINI Faculty of Applied Sciences and Biotechnology Shoolini UniversitySolan173229India Department of Biotechnology MMECMaharishi Markandeshwar(Deemed to be University)Mullana133207India Maharishi Markandeshwar University KumarhattiSolan173229India Department of Chemistry Physical SciencesMizoram UniversityAizawl796004India Department of Kinesiology California State UniversitySacramentoCA 95819USA Laboratory on the Mechanism and Regulation of Protein Synthesis Eunice K.ShriverNational Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaMD 20892USA

MicroRNA-153(miR-153),belongs toa dass of small non-coding *** is a aritical regulator of gene expression at the post-transcriptional lewel which interacts with the functional mRNA at 3UTR rgion and suppresses the expression of the *** recently,it has become apparent that dhanges in the miR-153 axpression lead to invasion,metastasis,angiogenesis and various types of tumor *** review summarizes the connection between dysrgulation of miR-153 and various typas of cancer ***-153 regulates various signaling pathways to inhibit the proliferation and induce apoptosis in the ancer cell and also show synergistic activity with anticancer *** addition to this,the oncogenic bchavior of miR-153 and their use as a potential biomarker in cancer was also reviewed.

关键词： Biomarker Cancer miR-153 Oncogenic Tumor suppressor

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Value of neutrophil-lymphocyte ratio in evaluating response to percutaneous catheter drainage in patients with acute pancreatitis

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World Journal of Clinical Cases 2022年第1期10卷 91-103页

作者： Pankaj Gupta Gaurav Chayan das Akash Bansal Jayanta Samanta Harshal S Mandavdhare Vishal Sharma Shano Naseem Vikas Gupta thakur Deen Yadav Usha Dutta Neelam Varma Manavjit Singh Sandhu Rakesh Kochhar Department of Radiodiagnosis Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Gastroenterology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Hematology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Surgical Gastroenterology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India

BACKGROUND Early prediction of response to percutaneous catheter drainage(PCD)of necrotic collections in acute pancreatitis(AP)using simple and objective tests is critical as it may determine patient *** role of white blood cell(WBC)count and neutrophil-lymphocyte ratio(NLR)has not been assessed as a tool of early prediction of PCD success and is the focus of this *** To assess the value of WBC and NLR in predicting response to PCD in *** This retrospective study comprised consecutive patients with AP who underwent PCD between June 2018 and December *** and fluid collections were classified according to the revised Atlanta classification and organ failure was defined according to the modified Marshall *** and NLR were monitored 24 h prior PCD(WBC-0/NLR-0)and 24 h(WBC-1/NLR-1),48 h(WBC-2/NLR-2)and 72 h(WBC-3/NLR-3)after *** was calculated by dividing the number of neutrophils by the number of *** association of success of PCD(defined as survival without the need for surgery)with WBC and NLR was *** trend of WBC and NLR was also assessed post *** One hundred fifty-five patients[median age 40±13.6(SD),64.5%males,53.5%severe AP]were included in the final *** was done for acute necrotic collection in 99(63.8%)patients and walled-off necrosis in 56(36.1%)*** pain to PCD interval was 24±69.89 *** was successful in 109 patients(group 1)and 46 patients(group 2)who failed to *** was no significant difference in the baseline characteristics between the two groups except the severity of AP and frequency of organ *** WBC and NLR showed an overall decreasing *** was a significant difference between WBC-0 and WBC-1(P=0.0001).WBC-1 and NLR-1 were significantly different between the two groups(P=0.048 and 0.003,respectively).The area under the curve of WBC-1 and NLR-1 for predicting the success of PCD was 0.602 and 0.682,*** a cut-off value of 9.87 fo

关键词： Acute pancreatitis Percutaneous catheter drainage White blood cell Neutrophil-lymphocyte ratio Necrotic collection

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Planktonic Scenario of the River Ganga&Yamuna at Prayagraj in COVID-19 Lockdown:A Case Study

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Journal of Fisheries Science 2022年第1期4卷 19-25页

作者： Kalpana Srivastava Jitendra Kumar Dharmnath Jha Venkatesh Ramrao thakur Vijay kumar Basanta kumar das ICAR-Central Inland Fisheries Research Institute Regional CentrePrayagrajUttar Pradesh211002India ICAR-Central Inland Fisheries Research Institute BarrackporeWest Bengal700120India

Ganga is the most prestigious river of *** COVID-19 lockdown may have forced us to stay indoors,but it has been boon for pollution-ridden Ganga and *** is tiny organisms drifting with water current,influenced by river physical and chemical *** lockdown anthropogenic factors were reduced which affected water and plankton *** samples were collected from the upstream of the river Ganga(Shankerghat,latitude 25030’28”N and longitude,81052’10”E)and Yamuna(near boat club,latitude 25024’29”N and longitude 81054’50”E)at Prayagraj,during national *** the before lockdown period(2019),total 28 planktonic taxa were recorded from the river Ganga,among them 10 taxa from Bacillariophyceae,15 from Chlorophyceae and 3 from *** during LD period total 54 genera with 86 species was recorded(Bacillariophyceae 10 taxa,Chlorophyceae 23 taxa,Myxophyceae 9 taxa,Euglenophyceae 2 taxa,Dianophyceae,1,Rotiferea 7 taxa,Protozoa 2 taxa).Various species of green algae were observed in this small period of lockdown,some species were not observed since a long,like Pediastrum tetras,Scenedesmus abundans,Ankistrodesmus fusiformis,and Brachionus *** species of phytoplankton and zooplankton were in reproductive phase because river was flowing silently,without any internal and external *** was more affected by anthropogenic activity and factory discharge than Yamuna So lack of chemicals in the water and minimum human interference favoured auto rejuvenation of Ganga in terms of plankton quality,diversity and reproduction *** type of environmental changes may stimulate for origin of new species and disappear or reappear of various aquatic species.

关键词： River Ganga and Yamuna Plankton Diversity COVID-19 Lockdown&Prayagraj

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Deccan volcanism and riff-related magmatic rocks of Kutch, western India

Deccan volcanism and riff-related magmatic rocks of Kutch, w...

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International Conference on Continental Volcanism

作者： D.K.Paul Arijit Ray B.das S.K.Patil S.K.Biswas Department of Geology Presidency CollegeCalcutta 700073India Department of Geology Presidency CollegeCalcutta 700073India Department of Geology Presidency CollegeCalcutta 700073India Indian Institute of Geomagnetism Alibagh 402201MaharashtraIndia Flat 201 C-WingISM House818AThakur VillageKandivali（E）Mumbai 400101India

＜正＞Deccan volcanic eruption started in northwest India. The westernmost on-land exposure of Deccan volcanic activity is found in the Kutch-Saurashtra region. Currently active volcanism at the Reunion Island is considered to be the remaining "tail" of the Deccan plume "head". The Kutch basin originated as a pericratonic rift in late Triassic. The rifting was aborted in Late Cretaceous. Alkaline rocks with pyroxenite, syenite, and lamprophyre occur in

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印度Ganga-meghna-Brahmaputra平原及周围地区与孟加拉国地下水砷污染及其对健康影响的19年研究

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中国地方病学杂志 2007年第1期26卷 43-47页

作者： Ahamed S Hossain MA Mukharjee A Sengupta MK das B Nayak B Pal A Mukherjee SC Pati S Dutta RN Saha KC Quamruzzaman Q Chakraborti D 付松波（译）孙殿军（审校）印度柯尔喀塔Jadavpur大学环境研究学院 700032 孟加拉围达卡大学统计研究与训练学院印度柯尔喀塔医学院神经科印度柯尔喀塔SSKM医院医学教育研究生学院妇产科印度柯尔喀塔热带病学研究院皮肤科孟加拉国Dhaka社区医院不详

基于近19年对印度Ganga—meghna—Brahmaputra（简称GMB）平原（面积569749km^2，人口超过5亿）地下水砷污染的调查，可以确定印度Ganga—Brahmaputra平原（包括北方邦、比哈尔邦、恰尔康得邦、西孟加拉邦、阿萨姆邦）和孟加拉国普遍受... 详细信息

基于近19年对印度Ganga—meghna—Brahmaputra（简称GMB）平原（面积569749km^2，人口超过5亿）地下水砷污染的调查，可以确定印度Ganga—Brahmaputra平原（包括北方邦、比哈尔邦、恰尔康得邦、西孟加拉邦、阿萨姆邦）和孟加拉国普遍受到砷的污染。近期的调查研究也表明，除米佐拉姆邦外，其他各邦的地下水也受到不同程度的砷污染。至今为止．已对印度各邦和孟加拧国的175000份和50808份压把井水样进行了含砷量检测。并组织了一支包括皮肤科、神经科和妇产科医生在内的有经验的专家组，对上述地区125000人进行了临床检查，其中9％患有砷导致的皮肤病损症状。至今共采集分析了60000份生物样品。包括发样、指甲、尿样及皮肤鳞屑，其中80％的样品含砷量超过允许值，很多人由此受到砷的亚临床损害。在上述砷暴露地区．19000名儿童经过检查，近1100人患有砷导致的皮肤病损。该调查结果表明．儿童比成人更容易受到砷的毒性损害。在这种状况下．应着力解决饮水安全问题来减轻砷的苊害．

关键词：砷饮水皮肤疾病神经毒性妊娠结局

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