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A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice

World Journal of Gastroenterology 2006年第2期12卷 306-312页

作者： Yuan-Xiang Zhou Maggie Yuk-Ting Lee James Ming-Him Ng mee-len chye Wing-Kin Yip Sze-Yong Zee Eric Lam Department of Botany The University of Hong Kong PokfulamRoad Hong Kong China Biotech Center Rutgers University 59 Dudley RoadNew Brunswick NJ 08901-8520 United States

AIM： To cost-effectively express the 23-ku pE2, the most promising subunit vaccine encoded by the E2 fragment comprising of the 3＇-portion of hepatitis E virus （HEV） open reading frame 2 （ORF2） in plastids of tobacco （Nicotiana tabacum cv. SR1）, to investigate the transgene expression and pE2 accumulation in plastids, and to evaluate the antigenic effect of the plastid-derived pE2 in mice. METHODS： Plastid-targeting vector pRB94-E2 containing the E2 fragment driven by rice psbA promoter was constructed. Upon delivery into tobacco plastids, this construct could initiate homologous recombination in psaB-trnfM and trnG-psbC fragments in plastid genome, and result in transgene inserted between the two fragments. The pRB94-E2 was delivered with a biolistic particle bombardment method, and the plastid-transformed plants were obtained following the regeneration of the bombarded leaf tissues on a spectinomycin-supplemented medium. Transplastomic status of the regenerated plants was confirmed by PCR and Southern blot analysis, transgene expression was investigated by Northern blot analysis, and accumulation of pE2 was measured by ELISA. Furthermore, protein extracts were used to immunize mice, and the presence of the pE2-reactive antibodies in serum samples of the immunized mice was studied by ELISA. RESULTS： Transplastomic lines confirmed by PCR and Southern blot analysis could actively transcribe the E2 mRNA. The pE2 polypeptide was accumulated to a level as high as 13.27 μg/g fresh leaves. The pE2 could stimulate the immunized mice to generate pE2-specific antibodies. CONCLUSION： HEV-E2 fragment can be inserted into the plastid genome and the recombinant pE2 antigen derived is antigenic in mice. Hence, plastids may be a novel source for cost-effective production of HEV vaccines.

关键词： Hepatitis E virus E2 Plastid transformation Vaccine Tobacco

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Arabidopsis acyl-CoA-Binding Protein 2 Enhances Tolerance to Cadmium-induced Oxidative Stress in Transgenic Plants

Arabidopsis acyl-CoA-Binding Protein 2 Enhances Tolerance to...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： mee-len chye School of Biological Sciences The University of Hong Kong

＜正＞Arabidopsis acyl-CoA-binding proteins ACBP1 to ACBP6 are conserved at the acyl-CoA-binding domain which confers binding to long-chain acyl-CoA esters. Plasma membrane-localized ACBP2 also contains

关键词： Acyl-CoA-binding protein oxidative stress

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Plant acyl-CoA-binding proteins are involved in stress and development

Plant acyl-CoA-binding proteins are involved in stress and d...

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第十三届全国植物基因组学大会

作者： mee-len chye School of Biological Sciences University of Hong KongPokfulamHong Kong

Acyl-CoA-binding proteins(ACBPs) contain a conserved acyl-CoA-binding domain which binds acyl-CoA esters and ACBPs facilitate intracellular lipid *** proteins have been characterized from mammals,yeast,Drosophila and ...

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Polyunsaturated linolenoyl-Co A modulates ERF-Ⅶ mediated hypoxia signaling in Arabidopsis

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Journal of Integrative Plant Biology 2020年第3期62卷 330-348页

作者： Ying Zhou Wei-Juan Tan Li-Juan Xie Hua Qi Yi-Cong Yang Li-Ping Huang Yong-Xia Lai Yi-Fang Tan De-Mian Zhou Lu-Jun Yu Qin-Fang Chen mee-len chye Shi Xiao State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China School of Biological Sciences The University of Hong KongPokfulamHong Kong 999077China

In plants,submergence from flooding causes hypoxia,which impairs energy production and affects plant growth,productivity,and *** Arabidopsis,hypoxia induces nuclear localization of the group VII ethylene-responsive transcription factor RELATED TO AP2.12(RAP2.12),following its dissociation from the plasma membrane-anchored ACYL-COA BINDING PRO-TEIN1(ACBP1)and ***,we show that polyunsaturated linolenoyl-Co A(18:3-Co A)regulates RAP2.12release from the plasma *** caused a significant increase in 18:3-Co A,but a significant decrease in 18:0-,18:1-,and 18:2-Co *** of 18:3-Co A promoted nuclear accumulation of the green fluorescent protein(GFP)fusions RAP2.12-GFP,HYPOXIA-RESPONSIVE ERF1-GFP,and RAP2.3-GFP,and enhanced transcript levels of hypoxia-responsive *** with decreased ACBP1 and ACBP2(acbp1 ACBP2-RNAi,produced by ACBP2 RNA interference in the acbp1 mutant)had reduced tolerance to hypoxia and impaired 18:3-Co A-induced expression of hypoxia-related *** knockout mutants and overexpression lines of LONG-CHAIN ACYL-COA SYNTHASE2(LACS2)and FATTY ACID DE-SATURASE 3(FAD3),the acyl-Co A pool size and 18:3-Co A levels were closely related to ERF-VII-mediated signaling and hypoxia *** findings demonstrate that polyunsaturation of long-chain acyl-Co As functions as important mechanism in the regulation of plant hypoxia signaling,by modulating ACBP–ERF-VII dynamics.

关键词： Arabidopsis ERF impaired

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兼具凝集素活性的芥菜几丁质酶BjCHI1

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生物工程学报 2002年第5期18卷 572-577页

作者：欧阳石文赵开军冯兰香 chye mee-len RAM Sathishkvmar 中国农业科学院蔬菜花卉研究所北京100081 香港大学植物系

利用毕赤酵母表达系统表达芥菜几丁质酶基因BjCHI1及其两个衍生基因BjCHI2和BjCHI3,获得相应的蛋白质。经FPLC纯化后 ,测定了 3种蛋白质的几丁质酶活性 ,发现它们均能降解CM chitin RBV和胶状几丁质。以CM chitin RBV为底物时的Km值分别... 详细信息

利用毕赤酵母表达系统表达芥菜几丁质酶基因BjCHI1及其两个衍生基因BjCHI2和BjCHI3,获得相应的蛋白质。经FPLC纯化后 ,测定了 3种蛋白质的几丁质酶活性 ,发现它们均能降解CM chitin RBV和胶状几丁质。以CM chitin RBV为底物时的Km值分别为 0 .799mg mL、0 .5 44mg mL和 0 .793mg mL ,差别甚微。而以胶状几丁质为底物时的Km值分别为 0 .2 81mg mL、0 .388mg mL和 1.6 43mg mL ,表现一定的差别 ,说明几丁质结合域影响了酶对不溶性底物的亲和力。 3种蛋白中 ,只有BjCHI1在 33μg mL以上浓度具有凝集素活性 ,而BjCHI2和BjCHI3的浓度即使高达 80 0 μg mL也无凝集素活性 ,表明 2个几丁质结合域是BjCHI1具有凝集素活性的必需条件。

关键词：凝集素活性芥菜几丁质酶 BjCHI1

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城市发展与住区转型:新加坡与中国的对话与展望

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北京规划建设 2024年第4期 204-212页

作者：殷彪张若曦天津大学建筑学院新加坡国立大学设计与工程学院厦门大学建筑与土木工程学院

YB:Singapore is renowned as the Garden *** your opinion,what aspects of Singapore's urban development currently demonstrate global exemplariness and cutting-edge features?新加坡有着“花园城市”的美称,您认为目前新加坡... 详细信息

关键词：花园城市住区新加坡城市

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An experimental study on shock wave mitigation capability of polyurea and shear thickening fluid based suspension pads

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Defence Technology（防务技术） 2018年第1期14卷 12-18页

作者： Andi Haris Heow Pueh Lee Vincent Beng chye Tan Department of Mechanical Engineering National University of Singapore

The present experimental study investigates shock wave mitigation capability of potentially new personal protective equipment(PPE) suspension pads made from polyurea and shear thickening fluid(STF).The shock tube test results show that when placed behind Twaron fabric systems with thickness ranging from 2 mm to 18 mm, the replacement of conventional flexible foam pad with STF and STF-infused foam pads with the same thickness of 20 mm greatly reduces the normalized peak pressure(by about 72% for each pad). However, this benefit is partially offset by a large increase in the normalized impulse(by about78% for the STF pad and 131% for the STF-infused foam pad) which may cause the shock wave mitigation performance of these two pads to become less effective. Interestingly, the use of 4 mm thick polyurea pad can greatly reduce the normalized peak pressure and impulse as well(by about 74% and 49%, respectively). These results reveal that among the potentially new suspension pads tested, the polyurea pad displays the best shock wave mitigation performance. Therefore, polyurea has potential for use as a suspension pad in personal protective equipment requiring shock wave mitigation capability such as fabric ballistic vests, bomb suits and combat helmets.

关键词： Shock wave mitigation Polyurea Shear thickening fluid Suspension pad Ballistic fabric Primary blast injuries

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PCSK9 and triglyceride-rich lipoprotein metabolism

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The Journal of Biomedical Research 2015年第6期29卷 429-436页

作者： Irena Druce Hussein Abujrad Teik chye Ooi Clinical Research Laboratory Division of Endocrinology and MetabolismDepartment of MedicineUniversity of Ottawa Chronic Disease Program Ottawa Hospital Research InstituteThe Ottawa Hospital

Pro-protein convertase subtilisin-kexin 9 （PCSK9） is known to affect low-density lipoprotein （LDL） metabolism, but there are indications from several lines of research that it may also influence the metabolism of other lipopro- teins, especially triglyceride-rich lipoproteins （TRL）. This review summarizes the current data on this possible role of PCSK9. A link between PCSK9 and TRL has been suggested through the demonstration of （1） a correlation between plasma PCSK9 and triglyceride （TG） levels in health and disease, （2） a correlation between plasma PCSK9 and markers of carbohydrate metabolism, which is closely related to TG metabolism, （3） an effect of TG-lowering fibrate therapy on plasma PCSK9 levels, （4） an effect of PCSK9 on postprandial lipemia, （5） an effect of PCSK9 on adipose tissue biology, （6） an effect of PCSK9 on apolipoprotein B production from the liver and intestines, （7） an effect of PCSK9 on receptors other than low density lipoprotein receptor （LDLR） that are involved in TRL metabolism, and （8） an effect of anti-PCSK9 therapy on serum TG levels. The underlying mechanisms are unclear but starting to emerge.

关键词： hyperlipidemia hypercholesterolemia molecular biology

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Study on the hypolipidemic properties of garlic polysaccharide in vitro and in normal mice as well as its dyslipidemia amelioration in type2 diabetes mice

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Food Bioscience 2022年第3期47卷 785-792页

作者： Chanyuan Xie Wei Gao Xue Li Shuangshuang Luo Fook Yee chye Faculty of Food Science and Nutrition Universiti Malaysia Sabah88400 Kota KinabaluSabahMalaysia School of Life Sciences and Food Engineering Hebei University of EngineeringHandan 056038HebeiPR China Key Laboratory of Natural Products and Functional Food Development in Handan City School of Life Sciences and Food EngineeringHebei University of EngineeringHandan 056038HebeiPR China Key Laboratory of Comprehensive Utilization of Plant Resources in Hebei Province Chenguang Biotech Group CO.LTDHandan 057250HebeiPR China Chenguang Biotech Group CO. LTDHandan 057250HebeiPR China

In the present work,garlic polysaccharide(GP)in lowering lipid based on in vitro and in vivo approaches were *** in vitro studies revealed significant effects of GP on cholesterol,sodium cholate,oil binding,and lipase ***,the liver index in the medium and high dose GP group was significantly lower than the control group in normal mice which relatively corresponded to the modulated effect of intestinal flora.A high dose of GP has markedly reduced the ratio of intestinal Firmicutes to Bacteroidetes and increased the abundance of gut probiotics ***,the high dose of GP also significantly reduced the levels of triglyceride and low-density lipoprotein cholesterol of diabetic mice indicating that GP could not only prevent hyperlipidemia of metabolic syndrome but also further ameliorate dyslipidemia of diabetes *** study provides new prospects for garlic polysaccharide as a hypolipidemic nutraceutical for the treatment of metabolic syndrome and type 2 diabetes.

关键词： Garlic polysaccharide Hypolipidemic Cholesterol Intestinal microflora Diabetes mice

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Rupture of the long head of the biceps brachii tendon near the musculotendinous junction in a young patient: A case report

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World Journal of Orthopedics 2020年第2期11卷 123-128页

作者： Xuan Liu Andrew Hwee chye Tan Department of Orthopaedic Surgery Singapore General Hospital

We report an unusual case of the long head of the biceps brachii tendon rupture near the musculotendinous junction in a young *** injury occurred in a young athlete during sports *** clinical presentation,surgical treatment,and technique with tenodesis using a unicortical button of the ruptured tendon were *** post-surgical recovery was uneventful,and the patient returned to sports in 6 *** treatment approach and surgical technique of the long head of biceps brachii rupture was reviewed and *** conclusion,surgical treatment of the long head of the biceps brachii tendon rupture with unicortical button tenodesis resulted in a favorable outcome in a young athlete.

关键词： Athlete Sport injury Shoulder Biceps tendon Tenodesis

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