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Proteome analysis of alfalfa roots in response to water deficit stress

Journal of Integrative Agriculture 2016年第6期15卷 1275-1285页

作者： rahman md.atikur Kim Yong-Goo Alam Iftekhar LIU Gong-she Lee Hyoshin Lee Jeung Joo Lee Byung-Hyun Division of Applied Life Sciences (BK21Plus) Institute of Agriculture & Life Sciences (IALS) Gyeongsang National University Jinju 660-701 Republic of Korea Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China National Institute of Biotechnology Dhaka-1349 Bangladesh Division of Forest Biotechnology Korea Forest Research Institute Suwon 441-847 Republic of Korea Department of Applied Biology Institute of Agriculture & Life Sciences (IALS) Gyeongsang National University Jinju 660-701 Republic of Korea

To evaluate the response of alfalfa to water deficit （WD） stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H202 content, lipid peroxidation, DPPH （1,1-diphenyl-2-picrylhydrazyl）-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis （2-DE）. A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase （APx2） and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid （ABA） biosynthesis, reactive oxygen species （ROS） regulation, transcription/translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.

关键词： alfalfa proteome root water deficit stress

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Impact of non-alcoholic fatty liver disease on coronavirus disease 2019: A systematic review

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World Journal of Hepatology 2024年第8期16卷 1185-1198页

作者： Abdul Moeed Muhammad Omar Larik Muhammad Ahmed Ali Fahim Hafsah Alim Ur rahman Lubna Najmi Mah I Kan Changez Muhammad Moiz Javed md Al Hasibuzzaman Department of Internal Medicine Dow University of Health SciencesKarachi 74200SindhPakistan Department of Internal Medicine Dow International Medical CollegeKarachi 74200SindhPakistan Department of Internal Medicine Ziauddin UniversityKarachi 75000SindhPakistan Department of Research Yale UniversityNew Haven 06520CTUnited States Department of Internal Medicine King Edward Medical UniversityLahore 54000PunjabPakistan Institute of Nutrition and Food Science University of DhakaDhaka 1000Bangladesh Department of Medicine The Affiliated Hospital of Medical School of Ningbo UniversityNingbo 315211Zhejiang ProvinceChina

BACKGROUND Many studies have revealed a link between non-alcoholic fatty liver disease(NA-FLD)and coronavirus disease 2019(COVID-19),making understanding the relationship between these two conditions an absolute *** To provide a qualitative synthesis on the currently present data evaluating COVID-19 and *** This systematic review was conducted in accordance with the guidelines pro-vided by preferred reporting items for systematic reviews and meta-analyses and the questionnaire utilized the population,intervention,comparison,and outcome *** search strategy was run on three separate databases,PubMed/MEDLINE,Scopus,and Cochrane Central,which were systematically searched from inception until March 2024 to select all relevant *** addition,***,***,and Google Scholar were searched to identify grey *** After retrieval of 11 studies,a total of 39282 patients data were *** was found in 11.5%and 9.4%of people in NAFLD and non-NAFLD *** all,23.2%of NAFLD patients and 22%of non-NAFLD admissions diagnosed with COVID-19 were admitted to the intensive care unit,with days of stay *** support ranged from 5%to 40.5%in the NAFLD cohort and from 3.1%to 20%in the non-NAFLD *** incidence of acute liver injury showed *** improvement on days 7 and 14 between the two classifications was *** stay ranged from 9.6 days to 18.8 days and 7.3 days to 16.4 days in the aforementioned cohorts respectively,with 73.3%and 76.3%of patients being *** rates *** Clinical outcomes except mortality consistently showed a worsening trend in patients with NAFLD and concomitant *** research in conducting prospective longitudinal studies is essential for a more powerful conclusion.

关键词： Non-alcoholic fatty liver disease Coronavirus disease 2019 Mechanical ventilation Intensive care unit Acute liver injury

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Overall Assessment of Heat Transfer for a Rarefied Flow in a Microchannel with Obstacles Using Lattice Boltzmann Method

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工程与科学中的计算机建模(英文) 2024年第1期138卷 273-299页

作者： Siham Hammid Khatir Naima Omolayo M.Ikumapayi Cheikh Kezrane Abdelkrim Liazid Jihad Asad Mokdad Hayawi rahman Farhan Lafta Rashid Naseer Ali Hussien Younes Menni Laboratory of Development in Mechanics and Materials(LDMM) Zian Achour UniversityDjelfaPB 3117Algeria Department of Technology University Center Salhi Ahmed Naama(Ctr.Univ.Naama)P.O.Box 66Naama45000Algeria Department of Mechanical and Mechatronics Engineering Afe Babalola University Ado Ekiti360101Nigeria Departement of Physics Faculty of ScienceAbou Bekr Belkaid UniversityTlemcen13000Algeria Department of Physics Faculty of Applied SciencesPalestine Technical University-KadoorieTulkarmPalestine Aeronautical Technical Engineering Al-Farahidi UniversityBaghdad10011Iraq Mechanical Engineering Department University of KerbalaKarbala56001Iraq Information and Communication Technology Research Group Scientific Research CenterAl-Ayen UniversityThi-QarIraq National University of Science and Technology Dhi QarIraq

The objective of this investigation is to assess the effect of obstacles on numerical heat transfer and fluid flow momentum in a rectangular microchannel(MC).Two distinct configurations were studied:one without obstacles and the other with alternating obstacles placed on the upper and lower *** research utilized the thermal lattice Boltzmann method(LBM),which solves the energy and momentum equations of fluids with the BGK approximation,implemented in a Python coding *** jump and slip velocity conditions were utilized in the simulation for the MC and extended to all obstacle *** study aims to analyze the rarefaction effect,with Knudsen numbers(Kn)of 0.012,0.02,and *** outcomes indicate that rarefaction has a significant impact on the velocity and temperature *** presence of nine obstacles led to slower fluid movement inside the microchannel MC,resulting in faster cooling at the *** MCs with obstacles,the rarefaction effect plays a crucial role in decreasing the Nusselt number(Nu)and skin friction coefficient(Cf).Furthermore,the study demonstrated that the obstacles played a crucial role in boosting fluid flow and heat transfer in the *** findings suggest that the examined configurations could have potential applications as cooling technologies in micro-electro-mechanical systems and microdevice applications.

关键词： Microfluid rarefied flow LBM microchannel Knudsen number numerical simulation

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Serum Immunoglobulin Concentrations in Juvenile Idiopathic Arthritis Cases during Active and Inactive Disease States

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Open Journal of Rheumatology and Autoimmune Diseases 2024年第2期14卷 49-59页

作者： Sajib Muhammad Rayhan Kamrul Laila Shahana A. rahman Consultant (Paediatrics) Kalkini Upazila Health Complex Dhaka Bangladesh Department of Paediatrics Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh Department of Paediatrics (on PRL) Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh

Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immunoglobulin levels in JIA patients are significantly higher than their control during the active state of disease. Methodology: This prospective observational study was conducted over a period of 18 months All the newly diagnosed oligo-articular and poly-articular JIA patients having active disease were included by purposive sampling. Data were collected by a semi-structured predesigned questionnaire. Result: Most of the study subjects (57.6%) belonged to age group > 3 - 9 years. Oligo JIA was diagnosed in 66.7% and poly JIA in 33.3% of JIA children. The difference in mean (±SD) ESR (33.52 ± 21.29 and 15.09 ± 7.71 mm in 1st hour) at active and inactive states was highly significant. Mean (±SD) difference of IgG, IgM and IgA in active and inactive states of disease were highly significant. Conclusion: Higher and abnormal levels of immunoglobulin (IgG, IgM, and IgA) were present among JIA patients in active disease state which became normal during inactive disease state after treatment.

关键词： JIA Oligoarticular JIA Polyarticular JIA Immunoglobulins Acitive Disease Inactive Disease

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Nature’s Pharmacy under Siege: Investigating Antibiotic Resistance Pattern in Endophytic Bacteria of Medicinal Plants

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Advances in Microbiology 2024年第4期14卷 183-208页

作者： Bonoshree Sarkar Afroza Sultana Nabila Nawar Binti Farhana Tasnim Chowdhury Sadia Afrin Mohammad Fahim Taibur rahman Atiqur rahman Infection Biology Laboratory Department of Biochemistry and Molecular Biology University of Dhaka Dhaka Bangladesh Department of Pathology University of Alabama at Birmingham Birmingham USA

Antibiotic resistance poses a significant global health threat, necessitating a thorough understanding of its prevalence in various ecological contexts. Medicinal plants, renowned for their therapeutic properties, host endophytic bacteria that produce bioactive compounds. Understanding antibiotic resistance dynamics in these bacteria is vital for human health and antibiotic efficacy preservation. In this study, we investigated antibiotic resistance profiles in endophytic bacteria from five medicinal plants: Thankuni, Neem, Aparajita, Joba, and Snake plant. We isolated and characterized 113 endophytic bacteria, with varying resistance patterns observed against multiple antibiotics. Notably, 53 strains were multidrug-resistant (mdR), with 14 exhibiting extensive drug resistance (XDR). Thankuni-associated bacteria displayed 44% mdR and 11% XDR, while Neem-associated bacteria showed higher resistance (60% mdR, 13% XDR). Aparajita-associated bacteria had lower resistance (22% mdR, 6% XDR), whereas Joba-associated bacteria exhibited substantial resistance (54% mdR, 14% XDR). Snake plant-associated bacteria showed 7% mdR and 4% XDR. Genus-specific distribution revealed Bacillus (47%), Staphylococcus (21%), and Klebsiella (11%) as major contributors to mdR. Our findings highlight diverse drug resistance patterns among plant-associated bacteria and underscore the complexity of antibiotic resistance dynamics in diverse plant environments. Identification of XDR strains emphasizes the severity of the antibiotic resistance problem, warranting further investigation into contributing factors.

关键词： Antibiotic Resistance Endophytic Bacteria Medicinal Plants Drug Resistance

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A Game-Theoretic Approach to Safe Crowd Evacuation in Emergencies

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Computers, Materials & Continua 2024年第4期79卷 1631-1657页

作者： Maria Gul Imran Ali Khan Gohar Zaman Atta rahman Jamaluddin Mir Sardar Asad Ali Biabani May IssaAldossary Mustafa Youldash Ashraf Saadeldeen Maqsood Mahmud Asiya Abdus Salam Dania Alkhulaifi Abdullah AlTurkey31Department of Computer Science COMSATS University IslamabadAbbottabad CampusAbbottabad22010Pakistan Department of Computer Science Abbottabad University of Science and TechnologyAbbottabad22010Pakistan Department of Computer Science College of Computer Science and Information TechnologyImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Science and Technology Unit Umm Al-Qura UniversityMakkah21955Saudi Arabia Deanship of Scientific Research Umm Al-Qura UniversityMakkah21955Saudi Arabia Department of Computer Information Systems College of Computer Science and Information TechnologyImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Department of Computer Engineering College of Computer Science and Information TechnologyImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Department of Computer Science Faculty of Computer Science and Information TechnologyOmdurman Islamic UniversityOmdurman14415Sudan Business Analytic Program Department of Management and MarketingCollege of Business AdministrationUniversity of BahrainSakhir32038Bahrain

Obstacle removal in crowd evacuation is critical to safety and the evacuation system efficiency. Recently, manyresearchers proposed game theoreticmodels to avoid and remove obstacles for crowd evacuation. Game theoreticalmodels aim to study and analyze the strategic behaviors of individuals within a crowd and their interactionsduring the evacuation. Game theoretical models have some limitations in the context of crowd evacuation. Thesemodels consider a group of individuals as homogeneous objects with the same goals, involve complex mathematicalformulation, and cannot model real-world scenarios such as panic, environmental information, crowds that movedynamically, etc. The proposed work presents a game theoretic model integrating an agent-based model to removethe obstacles from exits. The proposed model considered the parameters named: (1) obstacle size, length, andwidth, (2) removal time, (3) evacuation time, (4) crowd density, (5) obstacle identification, and (6) route *** proposed work conducts various experiments considering different conditions, such as obstacle types, obstacleremoval, and several obstacles. Evaluation results show the proposed model’s effectiveness compared with existingliterature in reducing the overall evacuation time, cell selection, and obstacle removal. The study is potentially usefulfor public safety situations such as emergency evacuations during disasters and calamities.

关键词： Safe crowd evacuation public safety emergency transition probability cooperation

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Pain Efficacy of a Home-Based Low-Intensity Continuous Ultrasound Stimulator for Knee Arthritis: A Single-Arm, Open-Label, Prospective Clinical Trial

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Health 2024年第7期16卷 703-716页

作者： md. Habibur rahman Yeon-Gyu Jang Johny Bajgai Subham Sharma Kchorng Vira Abdul-Nasir Sofian Seong Hoon Goh Yundeok Kim Cheol-Su Kim Doo-Sup Kim Kyu-Jae Lee Department of Convergence Medicine Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Neurosurgery Wonju Severance Christian Hospital Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Global Medical Science Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Internal Medicine Division of Hematology-Oncology Wonju Severance Christian Hospital Wonju Republic of Korea Department of Orthopaedic Surgery Wonju College of Medicine Yonsei University Wonju Republic of Korea

1) Background: Osteoarthritis (OA) is defined as a degenerative joint disease that mainly affects the bone. This study aims to evaluate the effect of low-intensity continuous ultrasound (LICUS) treatment on the knee of osteoarthritis patients through home-based intervention using the LICUS medical device. 2) Methods: The clinical trials were designed in a single-arm, open-label, and intervention study. Thirty-five participants, including those who dropped out (12%), were screened and enrolled. The patients received LICUS (1.1 MHz, 1.5 W/cm2, collimated beams) on the knee by the instructions of the investigator at home (5 min/session, 3 times/day, for four-weeks). Outcome measures were assessed using the Visual Analog Scale (VAS) as a primary endpoint and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) as a secondary endpoint to evaluate pain relief and functional recovery of the knee between pre-treatment (baseline) and post-treatment (four-weeks). 3) Results: Knee pain scores measured using the VAS and WOMAC indices were significantly reduced after a four-week treatment with LICUS compared to baseline. Knee stiffness and functional capacity were significantly reduced after the LICUS application. In addition, there were no reports of adverse effects during the study period. 4) Conclusion: Long-term and home-based application of LICUS can be recommended as an alternative option for the treatment of OA patients, as evidenced by the effect of pain relief and knee function recovery.

关键词： Knee Osteoarthritis Low-Intensity Continuous Ultrasound Stimulator Clinical Trial Visual Analogue Scale Western Ontario and McMaster Universities Osteoarthritis Index

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Assessment of Municipal Solid Waste Management in the Farmgate Area of Dhaka North City Corporation

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World Journal of Engineering and Technology 2024年第1期12卷 1-23页

作者： Seyedali Mirmotalebi Shoeb rahman Mayida Rubya Tithi Imran Khan Apu Department of Computational Data Science and Engineering North Carolina A&T State University Greensboro North Caro-lina USA Department of Civil Engineering & Construction Georgia Southern University Statesboro Georgia USA Department of Tropical Hydrogeology and Environmental Engineering Technical University of Darmstadt Darmstadt Ger-many Department of Civil Engineering European University of Bangladesh Dhaka Bangladesh

This investigation is focused on conducting a thorough analysis of Municipal Solid Waste Management (MSWM). MSWM encompasses a range of interdisciplinary measures that govern the various stages involved in managing unwanted or non-utilizable solid materials, commonly known as rubbish, trash, junk, refuse, and garbage. These stages include generation, storage, collection, recycling, transportation, handling, disposal, and monitoring. The waste materials mentioned in this context exhibit a wide range of items, such as organic waste from food and vegetables, paper, plastic, polyethylene, iron, tin cans, deceased animals, byproducts from demolition activities, manure, and various other discarded materials. This study aims to provide insights into the possibilities of enhancing solid waste management in the Farmgate area of Dhaka North City Corporation (DNCC). To accomplish this objective, the research examines the conventional waste management methods employed in this area. It conducts extensive field surveys, collecting valuable data through interviews with local residents and key individuals involved in waste management, such as waste collectors, dealers, intermediate dealers, recyclers, and shopkeepers. The results indicate that significant amounts of distinct waste categories are produced daily. These include food and vegetable waste, which amount to 52.1 tons/day;polythene and plastic, which total 4.5 tons/day;metal and tin-can waste, which amounts to 1.4 tons/day;and paper waste, which totals 5.9 tons/day. This study highlights the significance of promoting environmental consciousness to effectively shape the attitudes of urban residents toward waste disposal and management. It emphasizes the need for collaboration between authorities and researchers to improve the current waste management system.

关键词： Solid Waste Management Dhaka North City Corporation Sustainable Growth Integrated Waste Management Practice Waste Recycling

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Heat stress impairs floral meristem termination and fruit development by affecting the BR-SlCRCa cascade in tomato

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Plant Communications 2024年第4期5卷 208-223页

作者： Junqing Wu Pengxue Li Meng Li Danyang Zhu Haochuan Ma Huimin Xu Shuang Li Jinbo Wei Xinxin Bian Mengyao Wang Yixuan Lai Yuxin Peng Haixiao Li Abidur rahman Shuang Wu College of Horticulture Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Plant Bio-Sciences Faculty of AgricultureIwate UniversityMorioka 020-8550Japan United Graduate School of Agricultural Sciences Iwate UniversityMorioka 020-8550Japan These authors contributed equally to this article

Floral meristem termination is a key step leading to carpel initiation and fruit *** frequent occurrence of heat stress due to global warming often disruptsfloral determinacy,resulting in defective fruit ***,the detailed mechanism behind this phenomenon is largely ***,we identify CRABS CLAW a(SlCRCa)as a key regulator offloral meristem termination in *** func-tions as an indispensablefloral meristem terminator by suppressing SlWUS activity through the TOMATO AGAMOUS 1(TAG1)–KNUCKLES(SlKNU)–INHIBITOR OF MERISTEM ACTIVITY(SlIMA)network.A direct binding assay revealed that SlCRCa specifically binds to the promoter and second intron of WUSCHEL(SlWUS).We also demonstrate that SlCRCa expression depends on brassinosteroid homeostasis in theflo-ral meristem,which is repressed by heat stress via the circadian factor EARLY FLOWERING 3(SlELF3).These results provide new insights intofloral meristem termination and the heat stress response inflowers and fruits of tomato and suggest that SlCRCa provides a platform for multiple protein interactions that may epigenetically abrogate stem cell activity at the transition fromfloral meristem to carpel initiation.

关键词： heat stress floral meristem termination BR-CRC fruit development

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Endoscopic push through tragal cartilage tympanoplasty: A 10-year retrospective review of our technique and outcomes

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World Journal of Otorhinolaryngology 2024年第2期11卷 18-24页

作者： K M Abidur rahman Khalid Majeed Emma Finnegan Ivan Keogh Ear Noseand Throat/Head and Neck SurgeryGalway University HospitalGalway H91 YR71Ireland Academic Department of Otorhinolaryngology and Head and Neck Surgery University of GalwayGalway H91 TK33Ireland

BACKGROUND Endoscopic ear surgery(EES)provides a magnified,high-definition view of the otological surgical *** allows otologists to avoid surgical incisions and associated postoperative *** is an ideal technique for the perfor-mance and teaching of *** To examine the efficacy of total Endoscopic Push Through Tragal Cartilage Tympanoplasty(EPTTCT),at our institution over a 10-year *** A retrospective analysis of 168 cases of EPTTCT for closure of small to medium tympanic membrane perforations from 2013-2023 was *** sex,age range(pediatric vs adult),etiology of injury,success rate,complications,and postoperative hearing status were *** Graft uptake results indicated success in 94%of patients,with less than a 2%complication *** pure tone audiometry demonstrated hearing status improvement in 69%of *** EPTTCT has been shown to be effective in tympanic membrane perforation closures with minimal *** study further demonstrates the efficacy and safety of these procedures in a single-center review.

关键词： Total endoscopic ear surgery Otology Tympanoplasty Hearing Perforation Tragal cartilage graft

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