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Antibacterial Activity of Lactic Acid Bacteria and Extraction of Bacteriocin Protein

Advances in Bioscience and Biotechnology 2020年第2期11卷 49-59页

作者： Rabiul Islam md. Nur Hossain md. Khasrul Alam md. ekhlas uddin Mehadi Hasan Rony md. Abu Sayeed Imran md. Firoz Alam Divisional DNA Screening Laboratory Faridpur Medical College Hospital Ministry of Women & Children Affairs Faridpur Bangladesh Industrial Microbiology Laboratory Institute of Food Science & Technology Bangladesh Council of Scientific & Industrial research Dhaka Bangladesh Department of Biotechnology & Genetic Engineering Islamic University Kushtia Bangladesh Department of Biochemistry and Molecular Biology Gono Bishwabidyalay Dhaka Bangladesh

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. was isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identified by gram staining & colony morphology. Biochemically recognized by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determined by SDS PAGE method. The isolate was found to be facultative anaerobic, Gram positive, and catalase negative. The result of antimicrobial activity measured by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolates found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.

关键词： Lactobacillus spp. Bacteriocin Antimicrobial Activity SDS PAGE Biopreservative

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Isolation, Identification and Antimicrobial Susceptibility Profile Analysis of <i>Vibrio cholerae</i>O1 from Stool Samples of Bangladesh

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Advances in Microbiology 2018年第3期8卷 188-196页

作者： Muhammad ekhlas uddin Tanzina Akter Papia Sultana Papia Sultana md. Ikramul Hasan Mahbuba Akter Lubna Hasan Al Monem md. Anowar Khasru Parvez Shamsun Nahar md. Sumon Khan Department of Microbiology Primeasia University Dhaka Bangladesh National Institute of Biotechnology Dhaka Bangladesh Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Microbiology Stamford University Bangladesh Dhaka Bangladesh Department of Biotechnology and Genetic Engineering Jahangirnagar University Dhaka Bangladesh

Cholera is a severe diarrheal disease which is usually caused by toxigenic strain of Vibrio cholerae O1 and O139. Cholera is still one of the major health concerns in developing countries like Bangladesh due to poor sanitation and unavailability of safe drinking water. This experiment was confronted to identify V. cholerae O1 from stool samples as well as to determine the antibiotic susceptibility pattern of the isolated strains. A total of 140 stool samples from people infected with diarrheal disease were collected from July 2016 to December 2016. Among all, 58 samples were found positive for V. cholerae which were further subjected to sero-grouping by specific anti-sera and antimicrobial sus-ceptibility test by Kirby Bauer disc diffusion method. The zones of inhibition were measured and interpreted by following the recommendations of the criteria of Clinical and Laboratory Standards Institute (CLSI). It was found that 43 (74.1%) isolates of V. cholerae were O1 serogroup of Ogawa serotype and the rest 15 (25.9%) were O1 serogroup of Inaba serotype. People aged between 41 - 50 were most susceptible to V. cholerae O1 having about 39.7% of positive cases. The isolates were highly susceptible to Ciprofloxacin and Gentamicin with 100% susceptibility whereas 100% resistant was found towards Nalidixic acid. Though most of the isolates in our study were susceptible against tested antibiotics, the continuous surveillance is required to see the changing pattern of serogroups or serotypes and antimicrobial profile in this region.

关键词： Cholera Antibiotic Resistant Vibrio cholerae

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