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Comparative phytochemical,thin layer chromatographic profiling and antioxidant activity of extracts from some Indian herbal drugs

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Journal of Bioresources and Bioproducts 2022年第2期7卷 46-52页

作者： md.Sohail Akhtar Mohamed Rafiullah Wegdan Aly Shehata amzad hossain Mohammed Ali School of Pharmacy College of Pharmacy and NursingUniversity of NizwaNizwaOman Phytochemistry Research Laboratory Department of Pharmacognosy and PhytochemistryFaculty of PharmacyJamia HamdardNew DelhiIndia Strategic Center for Diabetes Research College of MedicineKing Saud UniversityRiyadhSaudi Arabia

Natural antioxidants play a significant role to prevent cell breaking and regenerate *** plants native to India named dwarf morning-glory(Evolvulus alsinoides),Indian banyan(Ficus bengalensis)and chicory(Chicorium intybus)are used as medicine in India traditionally to treat *** aim of this study is to evaluate and compare the antioxidant activity as well as to compare phytochemical profiles of the aqueous extracts of the selected three *** dried plant powder was extracted with ethanol individually by using the Soxhlet method for eight hours and the solvent was evaporated under reduced ***,the air-dried powder was ex-tracted with water,and the water was *** thin layer chromatography(TLC)profile of the extracts was determined by using TLC densitometer and the antioxidant activity was deter-mined by the conventional 2,2-diphenyl-1-picrylhydrazyl(DPPH)*** TLC profile shows that there are no similarities of retention factor(R f)values among the plant extracts which means all three plants contain different chemical *** antioxidant activities of the aqueous extracts at different concentrations show different antioxidant *** inhibition is dose dependent,as doses are increasing then percentage inhibition is also increasing for all three plant *** the selected plants,the highest activity was found in *** and the lowest was in *** order of the antioxidant activity among the selected plants was ***<***<*** on the TLC profile and antioxidant activities,the highest activity of the selected plant species was evaluated.

关键词： Indian drug Thin layer chromatography(TLC)profile Plant extract Antioxidant activity

Isolation and structural elucidation of antifungal compounds from Curcuma amada

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Asian Pacific Journal of Tropical Medicine 2019年第3期12卷 123-129页

作者： Jesmin Akter Kensaku Takara md.Zahorul Islam md.amzad hossain Ayako Sano De-Xing Hou The United Graduate School of Agricultural Sciences Kagoshima University Faculty of Agriculture University of the Ryukyus

Objective: To isolate and identify the antifungal compounds from Curcuma amada. Methods: The antifungal activity was measured by the diameter of colonies grown on Petri dish,microscopic observation,and CLSI microdilution methods. The antifungal compounds were isolated through bioactivity guided purification by using silica gel and high-performance liquid chromatography. Structural identification of the antifungal compounds was conducted using 1 H NMR,13 C NMR,and liquid chromatography-tandem mass spectrometry. Results: The purified antifungal compounds were zederone and furanodienone. These two compounds showed dose-dependent antifungal activity against Fusarium solani sensu lato. The concentration required for 50% growth inhibition(IC50) of FSSL ranged from 115 to 129 μM and 82 to 91 μM for zederone and furanodienone,respectively. Conclusion: This study suggested that the isolated compounds from Curcuma amada could be promising natural antifungal agents to control the diseases caused by Fusarium solani sensu lato.

关键词： Curcuma amada Fusarium solani sensu lato Antifungal compounds Zederone Furanodienone Nuclear magnetic resonance

Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 253-256页

作者： Yeasmeen Ali md.Ashraful Islam Nazmul Hasan Muzahid Mohd.Omar Faruk Sikder md.amzad hossain Lolo Wal Marzan Department of Genetic Engineering & Biotechnology University of Chittagong Graduate School of Biomedical Sciences Texas Tech University Health Sciences Center

Objective: To reveal the presence of methicillin resistant Staphylococcus aureus(S. aureus)(MRSA) in poultry samples and to determine the antibiogram pattern against five ***: Samples from different poultry farm of Chittagong city, Bangladesh were examined for S. aureus by different biochemical tests and confirmed as MRSA by identifying the presence of mec A gene using PCR. Antibiotic resistance pattern in S. aureus was determined by antibiotic disk diffusion ***: In this study, a total of 60 samples(30 from nasal swabs and 30 from cloacal swabs) were used, of which 54 were confirmed as S. aureus by different biochemical tests. Among these, 12 were confirmed as MRSA by detecting mec A gene using *** antibiogram study, both nasal and cloacal samples showed the highest resistance against penicillin-G and the lowest resistance was observed against ***: Based on the present study, it can be said that different antibiotics are used extensively in poultry that leads to MRSA and is alarming for human health.

关键词： Antibiogram Zoonotic transmission Swab mecA gene Opportunistic fungus

Copper and Mercury in Food, Biological and Pharmaceutical Samples: Spectrophotometric Estimation as Cu(DDTC)<sub>2</sub>

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American Journal of Analytical Chemistry 2014年第13期5卷 838-850页

作者： Mohammad Nasir Uddin Nur Mostaq Shah Mohammad amzad hossain md. Muzahidul Islam Department of Chemistry University of Chittagong Chittagong Bangladesh

An alternative spectrophotometric method was optimized and validated for the estimation of mercury using diethyldithiocarbamate (DDTC), a common reagent, widely used for the preconcentration and isolation of metal ions in complex matrices followed by their estimation by varied techniques. Diethyldithiocarbamate forms yellow Cu(DDTC)2 with copper and white Hg(DDTC)2 with mercury (having d10 system) which are extracted in CCl4. The UV-visible spectrum of Cu(DDTC)2 is very stable at pH 5.0 and has a maximum absorption (λmax) at 435 nm. Hg(DDTC)2 is more stable than Cu(DDTC)2. Estimation of mercury is based on a quantitative displacement of Cu(II) of Cu(DDTC)2 with the addition of mercury followed by the measurement of reduced absorbance. Primarily, method was optimized and validated for the estimation of copper. Therefore, simultaneous determination of Cu(II) and Hg(II) in mixture is proposed fractionating the extract. The molar specific coefficient (ε) for the mercury was 1.4 × 104 mol﹣1·L·cm﹣1 and for copper was 3.16 × 105 mol﹣1·L·cm﹣1 at 435 nm. The detection limits of Cu2+ and Hg2+ were 0.023 μg·mL﹣1 and 0.029 μg·mL﹣1, respectively. The calibration curve shows good linearity of 0.02 - 12.0 and 0.02 - 15.0 μg·mL﹣1 for the Cu2+ and Hg2+ determination, respectively. Proposed technique was applied to food, biological and pharmaceutical samples for the determination of Cu(II) and Hg(II).

关键词： Copper Mercury Diethyldithiocarbamate Spectrophotometric Method Food Biological Pharmaceutical Samples

Evaluation of in vitro antioxidant potential of different polarities stem crude extracts by different extraction methods of Adenium obesum

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Journal of Coastal Life Medicine 2014年第9期 699-703页

作者： Mohammad amzad hossain Tahiya Hilal Ali Alabri Amira Hamood Salim Al Musalami md.Sohail Akhtar Sadri Said School of Pharmacy College of Pharmacy and NursingUniversity of NizwaP.O.Box 33Postal Code 616NizwaSultanate of OmanOman

Objective:To select best extraction method for the isolated antioxidant compounds from the stems of Adenium ***:Two methods used for the extraction are Soxhlet and maceration *** solvent was used for both extraction *** methanol crude extract was defatted with water and extracted successively with hexane,chloroform,ethyl acetate and butanol *** antioxidant potential for all crude extracts were determined by using 1,1-diphenyl-2-picrylhydrazyl ***:The percentage of extraction yield by Soxhlet method is higher compared to maceration *** antioxidant potential for methanol and its derived fractions by Soxhlet extractor method was highest in ethyl acetate and lowest in hexane crude extracts and found in the order of ethyl acetate>butanol>water>chloroform>methanol>***,the antioxidant potential for methanol and its derived fractions by maceration method was highest in butanol and lowest in hexane followed in the order of butanol>methanol>chloroform>water>ethyl acetate>***:The results showed that isolate antioxidant compounds effected on the extraction method and condition of extraction.

关键词： Adenium obesum Extraction In vitro antioxidant potential DPPH UV-visible spectroscopy