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Comparative analysis of two nonlethal methods for the study of the gut bacterial communities in wild lizards

Integrative Zoology 2023年第6期18卷 1056-1071页

作者： mauricio HERNÁNDEZ Sergio ANCONA Stephanie HEREIRA-PACHECO Aníbal HDÍAZ DE LA VEGA-PÉREZ Yendi E.NAVARRO-NOYA Doctorado en Ciencias Biológicas Centro de Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Departamento de Ecología Evolutiva Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexico CityMexico Estación Científica la Malinche Centro de Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Consejo Nacional de Ciencia y Tecnología—Centro Tlaxcala de Biología de la Conducta Universidad Autónoma de TlaxcalaTlaxcalaMexico Laboratorio de Interacciones Bióticas Centro de Investigación en Ciencias BiológicasUniversidad Autónoma de TlaxcalaTlaxcalaMexico

Fecal samples or cloacal swabs are preferred over lethal dissections to study vertebrate gut microbiota for ethical reasons,but it remains unclear which nonlethal methods provide more accurate information about gut *** compared the bacterial communities of three gastrointestinal tract(GIT)segments,that is,stomach,small intestine(midgut),and rectum(hindgut)with the bacterial communities of the cloaca and feces in the mesquite lizard Sceloporus *** hindgut had the highest taxonomic and functional alpha diversity,followed by midgut and feces,whereas the stomach and cloaca showed the lowest *** taxonomic assemblages of the GIT segments at the phylum level were strongly correlated with those retrieved from feces and cloacal swabs(rs>0.84 in all cases).The turnover ratio of Amplicon Sequence Variants(ASVs)between midgut and hindgut and the feces was lower than the ratio between these segments and the *** than half of the core-ASVs in the midgut(24 of 32)and hindgut(58 of 97)were also found in feces,while less than 5 were found in the *** the ASVs level,however,the structure of the bacterial communities of the midgut and hindgut were similar to those detected in feces and *** findings suggest that fecal samples and cloacal swabs of spiny lizards provide a good approximation of the taxonomic assemblages and beta diversity of midgut and hindgut microbiota,while feces better represent the bacterial communities of the intestinal segments at a single nucleotide variation level than cloacal swabs.

关键词： animal microbiome cloacal swabs fecal samples intestinal tract reptile gut microbiome

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The Difficult Airway: A New Simplified Approach to an Old Complex Problem

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Open Journal of Anesthesiology 2017年第8期7卷 210-233页

作者： Iván mauricio Alvarado Arteaga Department of Anesthesiology Hospital de San Jose Bogotá Colombia

Difficult airway situations are a major cause of morbidity, mortality and malpractice claims in anesthetic practice and other critical care settings. Although infrequent, they are serious and complex situations where many variables interact. Among these variables, quick and accurate rescuer decision-making is a major outcome determinant. Emerging evidence, recent advances in technology and development of new devices have provided new tools to deal with the problem, but still, basic underlying principles of management remain. Based on the available evidence and expert consensus, multiple guidelines and algorithms have been published, but they may have limited applicability, contain confusing and ambiguous recommendations or address very specific clinical situations. This narrative review attempts to summarize, organize and consolidate current trends and supported recommendations of the updated guidelines in one practical, concise and unified guide, proposing a simple, intuitive and novel algorithm.

关键词： Anesthesia Airway Management Airway Obstruction Intubation-Intratracheal Pulmonary Ventilation Guideline Algorithms

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Understanding wound healing in obesity

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World Journal of Experimental Medicine 2024年第1期14卷 22-32页

作者： Asha Cotterell Michelle Griffin mauricio A Downer Jennifer B Parker Derrick Wan Michael T Longaker Division of Plastic and Reconstructive Surgery Stanford UniversityPalo AltoCA 94301United States Stanford University School of Medicine Stanford University School of MedicinePalo AltoCA 94301United States Department of Surgery Stanford University School of MedicineHagey Laboratory for Pediatric Regenerative MedicinePalo AltoCA 94301United States

Obesity has become more prevalent in the global *** is associated with the development of several diseases including diabetes mellitus,coronary heart disease,and metabolic *** are a multitude of factors impacted by obesity that may contribute to poor wound healing *** millions worldwide classified as obese,it is imperative to understand wound healing in these *** advances in the understanding of wound healing in both healthy and diabetic populations,much is unknown about wound healing in obese *** review examines the impact of obesity on wound healing and several animal models that may be used to broaden our understanding in this *** a growing portion of the population identifies as obese,understanding the underlying mechanisms and how to overcome poor wound healing is of the utmost importance.

关键词： Obesity Wound healing Adipokines Tissue fibrosis Diabetes Preclinical animal models Hypertrophic skin scarring Wound tension Metabolic syndrome

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Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis

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World Journal of Gastroenterology 2016年第48期22卷 10545-10556页

作者： Gloria Segarra Belén Cortina María Dolores mauricio Susana Novella Paloma Lluch Javier Navarrete-Navarro Inmaculada Noguera Pascual Medina Department of Physiology University of Valencia and Institute of Health Research INCLIVAHospital Clínico Universitario Valencia Department of Gastroenterology and Hepatology and Institute of Health Research INCLIVA Hospital Clínico Universitario Valencia

AIM To evaluate the effects of asymmetric dimethylarginine(ADMA) in renal arteries from portal hypertensive and cirrhotic *** Rat renal arteries from Sham(n = 15), pre-hepatic portal hypertension(PPVL; n = 15) and bile duct ligation and excision-induced cirrhosis(BDL; n = 15) were precontracted with norepinephrine, and additional contractions were induced with ADMA(10-6-10-3 mol/L), an endogenous inhibitor of nitric oxide(NO) synthase. Concentration-response curves to acetylcholine(1 × 10-9^(-3) × 10^(-6) mol/L) were determined in precontractedrenal artery segments with norepinephrine in the absence and in the presence of ADMA. Kidneys were collected to determine the protein expression and activity of dimethylarginine dimethylaminohydrolase(DDAH), an enzyme that catabolizes ADMA. RESULTS In renal arteries precontracted with norepinephrine, ADMA caused endothelium-dependent contractions. The pD 2 values to ADMA were similar in the Sham and PPVL groups(4.20 ± 0.08 and 4.11 ± 0.09, P > 0.05, respectively), but were lower than those of the BDL group(4.79 ± 0.16, P < 0.05). Acetylcholine-induced endothelium-dependent relaxation that did not differ, in terms of p D2 and maximal relaxation, among the 3 groups studied. Treatment with ADMA(3 × 10^(-4) mol/L) inhibited acetylcholine-induced relaxation in the 3 groups, but the inhibition was higher(P < 0.05) in the BDL group compared with that for the Sham and PPVL groups. The m RNA and protein expression of DDAH-1 were similar in kidneys from the three groups. Conversely, DDAH-2 expression was increased(P < 0.05) in PPVL and further enhanced(P < 0.05) in the BDL group. However, renal DDAH activity was significantly decreased in the BDL group. CONCLUSION Cirrhosis increased the inhibitory effect of ADMA on basal- and induced-release of NO in renal arteries, and decreased DDAH activity in the kidney.

关键词： Portal hypertension Cirrhosis Nitric oxide Asymmetric dimethylarginine Nitric oxide inhibitors Dimethylarginine dimethylaminohydrolase

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World Journal of Gastrointestinal Oncology 2013年第11期5卷 198-203页

作者： Osama E Rahma mauricio Burotto Luisa Matos Do Canto Alexandre A Germanos Bassem R Haddad John L Marshall Medical Oncology Branch National Cancer Institute the Lombardi Comprehensive Cancer Center Georgetown University Medical Center Whitman College the Ruesch Center for the Cure of GI Cancers Georgetown University Medical Center

We are reporting the longest disease-free interval ever published for colorectal cancer, using gene profiling to confirm the linkage of the primary and metastasis. This rare case reports on a patient with late recurrence of colorectal cancer in the lung 19 years after its initial diagnosis. We used high-resolution array CGH(aCGH) to analyze the genetic aberrations of both the primary rectal and the recurrent metastatic lung lesions. Interestingly, we found striking similarities between the two lesions, despite the 19 years disease-free *** addition, most of the genes that were previously reported to be associated with a high recurrence score showed copy number gains by aCGH in one or both lesions. Our findings suggest that aCGH may be a helpful tool in analyzing the origin of metastatic lesions and reflect the need for a better understanding of the characteristics of the rectal tumors with a late recurrence potential.

关键词： Colorectal cancer Genetic aberrations Delayed Recurrence High-resolution array CGH

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Irrigation's effect and applied selection on the fiber quality of Ethyl MethaneSulfonate(EMS)treated upland cotton(Gossypium hirsutum L.)

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Journal of Cotton Research 2018年第4期1卷 1-11页

作者： WITT Travis W ULLOA mauricio PELLETIER Mathew G. MENDU Venugopal RITCHIE Glen L. Plant and Soil Science Department Texas Tech University USDA-ARS PA CSRL Plant Stress and Germplasm Development Research USDA-ARS Cotton Production and Processing Research Laboratory

Background: Producing rainfed cotton(Gossypium hirsutum L.) with high fiber quality has been challenging in the Texas High Plains because of extended periods of insufficient rainfall during sensitive boll developmental *** variation created by Ethyl MethaneSulfonate(EMS) mutagen has successfully improved fiber quality of cotton. However, little is known about the effect of water deficit environments on fiber quality. Three EMS treated populations were advanced from the first to the fourth generation(M1 to M4) as bulk harvested populations. In2014, single-plant divergent selection was applied based on perceived morphological and agronomic differences seen during and at the end of the ***: Analyses from these selections in 2014-2016 showed significant(P< 0.05) improvement between and within populations for fiber traits(micronaire, length, strength, uniformity, and elongation) when compared with the original non-treated EMS source; some selections were found to have excellent fiber quality under diverse ***: Some of these selections are being considered for germplasm release and could be useful for improving the fiber quality of cotton under water limited conditions, thereby helping to ensure the long-term survival of the cotton industry on the Texas High Plains.

关键词： Breeding Drought EMS Ethyl MethaneSulfonate Fiber quality Selection

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Arterial spin labeling in neuroimaging

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World Journal of Radiology 2010年第10期2卷 384-398页

作者： Sasitorn Petcharunpaisan Joana Ramalho mauricio Castillo Division of Neuroradiology University of North Carolina at Chapel HillRoom 3326Old Infirmary BuildingManning DriveChapel HillNC 27599-7510United States Department of Radiology King Chulalongkorn Memorial Hospital1873 Rama IV RoadPathumwanBangkok10330Thailand Department of Neuroradiology Centro Hospitalar de Lisboa CentralEPERua JoséAntónio Serrano1150-199 LisbonPortugal

Arterial spin labeling(ASL) is a magnetic resonance imaging technique for measuring tissue perfusion using a freely diffusible intrinsic *** compared with other perfusion techniques,ASL offers several advantages and is now available for routine clinical practice in many *** noninvasive nature and ability to quantitatively measure tissue perfusion make ASL ideal for research and clinical *** technical advances have increased its sensitivity and also extended its potential *** review focuses on some basic knowledge of ASL perfusion,emerging techniques and clinical applications in neuroimaging.

关键词： Arterial spin labeling Continuous arterial spin labeling Pulsed arterial spin labeling Pseudocontinuous arterial spin labeling Velocity-selective arterial spin labeling Territorial arterial spin labeling Arterial spin labeling at multiple inversion times Perfusion-based functional magnetic resonance imaging

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Scientometric Analysis of Research Output from Brazil in Response to the Zika Crisis Using e-Lattes

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Journal of Data and Information Science 2020年第4期5卷 137-146页

作者： Ricardo Barros Sampaio Antônio de Abreu Batista-Jr Bruno Santos Ferreira mauricio LBarreto Jesús PMena-Chalco Regional Board of Brasília Fiocruz Distrito FederalBrazil Department of Informatics Federal University of Maranhão São LuísMABrazil Department of Teaching and Learning Studies Laval UniversityQuébecCanada Center of Integrated Data and Knowledge about Health(CIDACS) SalvadorBrazil Center for Mathematics Computation and CognitionFederal University of ABC Santo AndréSPBrazil

Purpose:This paper aims to test the use of e-Lattes to map the Brazilian scientific output in a recent research health subject:Zika ***/methodology/approach:From a set of Lattes CVs of Zika researchers registered on the Lattes Platform,we used the e-Lattes to map the Brazilian scientific response to the Zika ***:Brazilian science articulated quickly during the public health emergency of international concern(PHEIC)due to the creation of mechanisms to streamline funding of scientific *** limitations:We did not assess any dimension of research quality,including the scientific impact and societal *** implications:e-Lattes can provide useful guidelines for different stakeholders in research groups from Lattes CVs of ***/value:The information included in Lattes CVs permits us to assess science from a broader perspective taking into account not only scientific research production but also the training of human resources and scientific collaboration.

关键词： e-Lattes Zika virus Lattes Platform Brazilian research output Brazilian science

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Adequation of a Virtual Immersive Environment for a Learning-Teaching Interaction Using the Limem 3d Method

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通讯和计算机（中英文版） 2015年第2期12卷 57-66页

作者： Diego mauricio Torres Arias Helmuth Trefftz Department of software engineer Universidad EAFIT Department of Computer and Systems Laboratory of Virtual Reality Universidad EAFIT Medellin Colombia

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Solar Driven Wind Speed Monitoring System Using Wireless or Wired Sensors

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Energy and Power Engineering 2014年第9期6卷 213-221页

作者： Federico Hahn mauricio Pablo José Reyes Department of Irrigation Universidad Autonoma Chapingo Texcoco México

Instrumented towers are being constructed to characterize vertical wind profiles in order to improve the understanding and characterization of a desired environment up to 100 m. The site being measured is at a height of 2500 m over sea level, in one side of Sierra Madre Oriental, which crosses Mexico from North to South. As the site has no energy, it was powered by a photovoltaic system. Power consumption of two sets of sensors fixed at the tower was evaluated. The first sensor set consisted of a pulsed anemometer and a RH sensor having a 0 - 10 V output;these sensors used cables for transmitting the output signals. Three cup anemometers fixed at 25, 35 and 45 m high measured wind speed. Wireless sensors in the second set, requires of a power consumption and battery life study. Solar cells energized the sensors, becoming the installation and operation easier. The datalogger that acquired the RH and T measurements encountered an 11% voltage loss throughout the cable;cup anemometer measurements did not show variations due to its pulsed signals. Wireless sensors drew less energy from the PV system, resulting in battery overcharge. A dump regulator turned-on a 200 W lamp during the night when the battery voltage reached 14 V;the lamp was turned-off when the battery voltage felt beneath 11.5 V. Considering the high wind available, wireless sensors batteries were charged by small wind turbines combined with 5 W solar panels.

关键词： Wind Tower Wireless Anemometers Battery Regulator

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