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Effect of Aspergillus niger prolyl endopeptidase in patients with celiac disease on a long-term gluten-free diet

World Journal of Gastroenterology 2024年第11期30卷 1545-1555页

作者： Juan Pablo Stefanolo Verónica Segura Martina Grizzuti Abel Heredia Isabel comino Ana Florencia costa Roberto Puebla María Paz Temprano Sonia Isabel Niveloni Gabriel de Diego María E Oregui Edgardo Gustavo Smecuol mauricio c de marzi Elena F Verdú carolina Sousa Julio césar Bai Small Bowel Section Department of MedicineGastroenterology Hospital of Buenos Aires“Dr.C.Bonorino Udaondo”Buenos Aires 1264Argentina department of Microbiology and Parasitology Faculty of PharmacyUniversity of SevilleSeville 41080Spain department of Medicine Dr.C.Bonorino Udaondo Gastroenterology HospitalBuenos Aires 1264Argentina Basic and Applied Research Group in Immunology and Bioactives(GIBAIB) Institute of Ecology and Sustainable Development(INEDES)National University of LujanLuján 6700Buenos AiresArgentina department of Medicine Farncombe Family Digestive Health Research InstituteMcMaster UniversityHamilton L8S 4L8OntarioCanada Facultad de Medicina Universidad del SalvadorBuenos Aires C1051ABBArgentina

BAcKGROUND The gluten-free diet(GFD)has limitations,and there is intense research in the development of adjuvant *** To examine the effects of orally administered Aspergillus niger prolyl endopeptidase protease(AN-PEP)on inadvertent gluten exposure and symptom prevention in adult celiac disease(ceD)patients following their usual *** This was an exploratory,double-blind,randomized,placebo-controlled trial that enrolled ceD patients on a long-term *** a 4-wk run-in period,patients were randomized to 4 wk of two AN-PEP capsules(GliadinX;AVI Research,LLc,United States)at each of three meals per day or *** endpoints were:(1)Average weekly stool gluten immunogenic peptides(GIP)between the run-in and end of treatments and between AN-PEP and placebo;(2)celiac symptom index(cSI);(3)ceD-specific serology;and(4)quality of *** samples were collected for GIP testing by ELISA every Tuesday and Friday during run-ins and *** Forty patients were randomized for the intention-to-treat analysis,and three were excluded from the per-protocol ***,628/640(98.1%)stool samples were *** was undetectable(ces between treatment arms were *** 0.5%of samples had GIP concentrations sufficiently high(>0.32μg/g)to potentially cause mucosal *** GIP concentration in the AN-PEP arm was 44.7%lower than in the run-in ***-third of patients exhibiting GIP>0.08μg/g during run-in had lower or undetectable GIP after AN-PEP *** with the run-in period,the proportion of symptomatic patients(cSI>38)in the AN-PEP arm was significantly lower(Pchanges in specific *** This exploratory study conducted in a real-life setting revealed high adherence to the *** AN-PEP treatment did not significantly reduce the overall GIP stool ***,given the observation of a significantly lower prevale

关键词： celiac disease Aspergillus niger prolyl endoprotease Gluten immunogenic peptides Trial Symptoms Real-life trial

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Low-power SiPM readout BETA ASIc for space applications

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Nuclear Science and Techniques 2024年第3期35卷 153-169页

作者： Anand Sanmukh Sergio Gómez Albert comerma Joan mauricio Rafel Manera Andreu Sanuy Daniel Guberman Roger catala Albert Espinya Marina Orta Oscar de la Torre David Gascon departament de Física Quàntica i Astrofísica(FQA) Institut de Ciències del Cosmos(ICCUB)Universitat de Barcelona(UB)08028 BarcelonaSpain Institut d’Estudis Espacials de catalunya(IEEc) 08034 BarcelonaSpain Electronics department Serra Hunter fellow at Polytechnic University of Catalonia(UPC)08019 BarcelonaSpain

The BETA application-specific integrated circuit(ASIc)is a fully programmable chip designed to amplify,shape and digitize the signal of up to 64 Silicon photomultiplier(SiPM)channels,with a power consumption of approximately~1 mW/*** to its dual-path gain,the BETA chip is capable of resolving single photoelectrons(phes)with a signal-to-noise ratio(SNR)>5 while simultaneously achieving a dynamic range of~4000 ***,BETA can provide a cost-effective solution for the readout of SiPMs in space missions and other applications with a maximum rate below 10 *** this study,we describe the key characteristics of the BETA ASIc and present an evaluation of the performance of its 16-channel version,which is implemented using 130 nm *** ASIc also contains two discriminators that can provide trigger signals with a time jitter down to 400 ps FWHM for 10 *** linearity error of the charge gain measurement was less than 2%for a dynamic range as large as 15 bits.

关键词： Radiation detectors Silicon photomultipliers Photon sensors Front-end electronics Mixed-mode ASIcs Space technology

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DNA metabarcoding reveals seasonal changes in diet composition across four arthropod-eating lizard species(Phrynosomatidae:Sceloporus)

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Integrative Zoology 2024年第3期19卷 480-495页

作者： mauricio HERNÁNdeZ Stephanie HEREIRA-PAcHEcO Antton ALBERDI Aníbal H.DÍAZ de LA VEGA-PÉREZ Arturo ESTRADA-TORRES Sergio ANcONA Yendi E.NAVARRO-NOYA Doctorado en ciencias Biológicas Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Estación científica La Malinche Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico center for Evolutionary Hologenomics The Globe InstituteUniversity of CopenhagenCopenhagenDenmark consejo Nacional de ciencia y Tecnología-centro Tlaxcala de Biología de la conducta Universidad Autónoma de TlaxcalaTlaxcalaMexico departamento de Ecología Evolutiva Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexico CityMexico Laboratorio de Interacciones Bióticas Centro de Investigación en Ciencias BiológicasUniversidad Autónoma de TlaxcalaSan Felipe IxtacuixtlaTlaxcalaMexico

Diet composition and its ecological drivers are rarely investigated in coexisting closely related *** used a molecular approach to characterize the seasonal variation in diet composition in four spiny lizard species inhabiting a mountainous *** metabarcoding revealed that the lizards Sceloporus aeneus,***,***,and *** mostly consumed arthropods of the orders Hemiptera,Araneae,Hymenoptera,and *** terrestrial lizards *** and *** mostly predated ants and spiders,whereas the arboreal–saxicolous *** and saxicolous *** largely consumed grasshoppers and *** taxonomic and phylogenetic diversity of the prey was higher during the dry season than the rainy season,likely because reduced prey availability in the dry season forced lizards to diversify their diets to meet their nutritional *** and phylogenetic composition varied seasonally depending on the species,but only dietary composition varied with *** dietary turnover was greater in *** than in ***,suggesting site-specific seasonal variability in prey availability;no other differences among species were ***,which lives at the highest altitude in our study site,displayed interseasonal variation in diet *** differences were correlated with the species’feeding strategies and elevational distribution,which likely contributed to the coexistence of these lizard species in the studied geographic area and beyond.

关键词： arthropod-eating lizards diet breadth environmental barcoding mountainous ecosystem seasonal dietary shifts

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Transarterial embolization is an acceptable bridging therapy to hepatocellular carcinoma prior to liver transplantation

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World Journal of Transplantation 2024年第2期14卷 126-134页

作者： Gabriel Lazzarotto-da-Silva Leandro A Scaffaro mauricio Farenzena Lucas Prediger Rafaela K Silva Flávia Heinz Feier Tomaz J M Grezzana-Filho Pablo D Rodrigues Alexandre de Araujo Mario Reis Alvares-da-Silva Roberta c Marchiori cleber Rosito Pinto Kruel Marcio Fernandes chedid department of Liver Transplant and Hepatobiliary Surgery Unit Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil department of Interventional Radiology Unit Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil department of Gastroenterology and Hepatology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil

BAcKGROUND Hepatocellular carcinoma(Hcc)is an aggressive malignant neoplasm that requires liver transplantation(LT).despite patients with Hcc being prioritized by most organ allocation systems worldwide,they still have to wait for long *** therapies(LRTs)are employed as bridging therapies in patients with Hcc awaiting *** largely used in the past,transarterial embolization(TAE)has been replaced by transarterial chemoembolization(TAcE).However,the superiority of TAcE over TAE has not been consistently shown in the *** To compare the outcomes of TAcE and TAE in patients with Hcc awaiting *** All consecutive patients with Hcc awaiting LT between 2011 and 2020 at a single center were *** patients underwent LRT with either TAcE or *** patients also underwent percutaneous ethanol injection(PEI),concom-itantly or in different treatment *** choice of LRT for each Hcc nodule was determined by a multidisciplinary *** primary outcome was waitlist dropout due to tumor progression,and the secondary outcome was the occurrence of adverse *** the subset of patients who underwent LT,complete pathological response and post-transplant recurrence-free survival were also *** Twelve(18.5%)patients in the TAcE group(only TAcE and TAcE+PEI;n=65)and 3(7.9%)patients in the TAE group(only TAE and TAE+PEI;n=38)dropped out of the waitlist due to tumor progression(P log-rank test=0.29).Adverse events occurred in 8(12.3%)and 2(5.3%)patients in the TAcE and TAE groups,respectively(P=0.316).Forty-eight(73.8%)of the 65 patients in the TAcE group and 29(76.3%)of the 38 patients in the TAE group underwent LT(P=0.818).Among these patients,complete pathological response was detected in 7(14.6%)and 9(31%)patients in the TAcE and TAE groups,respectively(P=0.145).Post-LT,Hcc recurred in 9(18.8%)and 4(13.8%)patients in the TAcE and TAE groups,respectively(P=0.756).Posttransplant recurrence-free survival was simila

关键词： Hepatocellular carcinoma Transarterial embolization Transarterial chemoembolization Liver transplantation Locoregional therapy Bridging

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Impact of STAT-signaling pathway on cancer-associated fibroblasts in colorectal cancer and its role in immunosuppression

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1705-1724页

作者： Damián Sánchez-Ramírez Mónica G Mendoza-Rodríguez Omar R Alemán Fernando A candanedo-González Miriam Rodríguez-Sosa Juan JoséMontesinos-Montesinos mauricio Salcedo Ismael Brito-Toledo Felipe Vaca-Paniagua Luis I Terrazas Unidad de Investigacion en Biomedicina Facultad de Estudios Superiores IztacalaUniversidad Nacional Autonoma de MexicoTlalnepantla 54090Estado de MexicoMexico department of Biology Facultad de QuimicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaMexico City 04510Mexico department of Pathology National Medical Center Century XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Laboratorio de células Troncales Mesenquimales Unidad de Investigación Médica en Enfermedades OncológicasHospital de Oncología Centro Medico Nacional Siglo XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Unidad de Investigacion en Biomedicina y Oncologia Genomica Instituto Mexciano del Seguro SocialMexico City 07300Mexico Servicio de colon y Recto Hospital de Oncología Centro Medico Nacional Siglo XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Laboratorio Nacional en Salud Facultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla 54090Estado de MexicoMexico

colorectal cancer(cRc)remains one of the most commonly diagnosed and deadliest types of cancer *** displays a desmoplastic reaction(DR)that has been inversely associated with poor prognosis;less DR is associated with a better *** reaction generates excessive connective tissue,in which cancer-associated fibroblasts(cAFs)are critical cells that form a part of the tumor *** are directly involved in tumorigenesis through different ***,their role in immunosuppression in cRc is not well understood,and the precise role of signal transducers and activators of transcription(STATs)in mediating cAF activity in cRc remains *** the myriad chemical and biological factors that affect cAFs,different cytokines mediate their function by activating STAT signaling ***,the harmful effects of cAFs in favoring tumor growth and invasion may be modulated using STAT ***,we analyze the impact of different STATs on cAF activity and their immunoregulatory role.

关键词： cancer-associated fibroblasts Signal transducer and activator of transcription signaling colorectal cancer Immunity Immunosuppression

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Feeding calcium Salts of Linseed Oil on Metabolic Status and Reproductive Traits in Grazing Dairy cows

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Open Journal of Animal Sciences 2024年第3期14卷 219-233页

作者： Jesica Daniela Iorio Yaliska Moreno González Maria Florencia Olmeda Dino curletto Daniel Edgardo Scandolo Martin Guillermo Maciel Rafael Alejandro Palladino mauricio Javier Giuliodori Eloy Eduardo Salado School of Agriculture of the University of Buenos Aires (FAUBA) Buenos Aires Argentina Faculty of Agricultural Sciences National University of Lomas de Zamora (UNLZ) Lomas de Zamora Argentina Rafaela Agricultural Experiment Station National Institute of Agricultural Technology (INTA) Rafaela Argentina Faculty of Veterinary Science La Plata National University (FCV) La Plata Argentina

This experiment aimed to evaluate the effect of calcium salts of linseed oil (rich in alpha-linolenic acid (ALA, c18:3n-3) on metabolic and reproductive traits in high-producing dairy cows under grazing. Thirty-six Holstein dairy cows were randomly assigned, in a complete block design, to receive ALA supplementation (0.85 kg∙day−1 of calcium salts of linseed oil) or to remain as untreated control (cON). The concentrate was formulated to offer the same amount of energy across treatments (cON cows received an extra kg of corn to compensate for the higher energy density of ALA treatment). A PMR + Alfalfa pasture was offered to all cows at the same time. A fixed time artiﬁcial insemination (FTAI) at 80 DIM, preceded by a Presynch plus Ovsynch protocol was implemented for the first service and later, on return to estrus, heat detection and artiﬁcial insemination (AI) were performed. Pregnancy diagnosis was checked at 30, 42, 60, and 90 d after AI. Blood and milk samples were taken biweekly. Treatment affected plasma cholesterol concentration (160.36 vs. 186.70 mg∙dl−1, p = 0.03, for ALA and cON, respectively) and on size of corpus luteum (cL, 17.6 vs. 13.7 mm, p = 0.02, for ALA and cON, respectively). Supplementation tended (p = 0.136) to increase conception rate by 200 DIM (81.69% vs. 55.43% in ALA and cON cows, respectively). However, treatment had no effect (p > 0.05) on body weight (BW), body condition score (BcS), and circulating levels of beta-hydroxybutyrate (BHBA), glucose, insulin, growth hormone (GH) and insulin-like growth factor (IGF-I). Our results suggest that supplementation with calcium salts of linseed oil could enhance ovarian function without affecting energy metabolism in early lactation dairy cows.

关键词： α-Linolenic Acid Supplementation Reproduction Grazing Dairy cows

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cluster Partition Function and Invariants of 3-Manifolds

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chinese Annals of Mathematics,Series B 2017年第4期38卷 937-962页

作者： mauricio ROMO School of-Natural Sciences Institute for Advanced Study Princeton NJ 08540 USA.

The author reviews some recent developments in chern-Simons theory on a hyperbolic 3-manifold M with complex gauge group G. The author focuses on the case of G = SL(N, c) and M being a knot complement: M = S^3\ K. The main result presented in this note is the cluster partition function, a computational tool that uses cluster algebra techniques to evaluate the chern-Simons path integral for G = SL(N, c). He also reviews various applications and open questions regarding the cluster partition function and some of its relation with string theory.

关键词： chern-Simons theory Knots cluster algebras

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Validation of cFD-deM simulation of a liquid-solid fluidized bed by dynamic analysis of time series

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Particuology 2022年第9期20卷 75-87页

作者： Julia Picabea mauricio Maestri Miryan cassanello Gabriel Salierno cataldo de Blasio María Angélica cardona Daniel Hojman Héctor Somacal departamento de Industrias Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresCiudad UniversitariaIntendente Güiraldes 2160C1428EGABuenos AiresArgentina cONIcET-Universidad de Buenos Aires Instituto de Tecnología de Alimentos y Procesos Químicos-ITAPROQCiudad UniversitariaIntendente Güiraldes 2160 C1428EGABuenos AiresArgentina Faculty of Science and Engineering Abo Akademi UniversityRantakatu 265100 VaasaFinland Laboratorio de Diagnósticos por Radiaciones Dep.Física ExperimentalComisión Nacional de Energía AtómicaBKNA1650 San MartínBuenos AiresArgentina cONIcET C1033AAJ Buenos AiresArgentina Escuela de ciencia y Tecnología Universidad de San MartínBKNA 1650 San MartínBuenos AiresArgentina

Liquid-solid fluidized beds(LSFB)modeling validation is crucial for establishing design rules and monitor-ing ***,it generally relies on comparing global variables,which overlook dynamic features that influence reaction *** work aims to implement time series analysis tools to compare Radioactive Particle Tracking data with a simulation consisting of computational Fluid Dynamics cou-pled with Discrete-Element *** have been performed in a pilot-scale LSFB of calcium alginate spheres fluidized with a calcium chloride *** Diks'test indicates that the simulation can capture the LSFB *** also allows diagnosing flow regime transitions from the *** of solid dispersion coefficients and mixing times predicted by the simulation are in good agreement with the experiments.

关键词： Liquid-solid fluidization cFD-deM Dynamic comparison Radioactive particle tracking

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Mitochondrial phylogeography and molecular evolution of the rhodopsin visual pigment in troglobitic populations of Astyanax mexicanus(de Filippi,1853)

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Zoological Research 2023年第4期44卷 761-775页

作者： Marco A.A.Garduño-Sánchez Vladimir de Jesus-Bonilla Silvia Perea Ramses Miranda-Gamboa Andrea Herrera-García mauricio de la Maza Benignos claudia Patricia Ornelas-García colección Nacional de Peces Departamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoC.P.04510México Posgrado en ciencias Biológicas Colección Nacional de PecesDepartamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoC.P.04510México Licenciatura en ciencias Forenses Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoCiudad UniversitariaCoyoacánMéxico CityC.P.04510México Instituto de Energías Renovables Universidad Nacional Autónoma de MéxicoTemixcoMorelos C.P.62580Mexico Facultad de ciencias Biológicas Universidad Autónoma de Nuevo LeónCiudad UniversitariaSan Nicolás de los GarzaNuevo LeónC.P.66450México

cave-adapted animals provide a unique opportunity to study the evolutionary mechanisms underlying phenotypic,metabolic,behavioral,and genetic evolution in response to cave *** Mexican tetra(Astyanax mexicanus)is considered a unique model system as it shows both surface and cave-dwelling *** date,at least 33 different cave populations have been identified,with phylogenetic studies suggesting an origin from at least two independent surface lineages,thereby providing a unique opportunity to study parallel *** the present study,we carried out the most exhaustive phylogeographic study of *** to date,including cave and surface localities,using two mitochondrial markers(cytochrome b(cyt b)and cytochrome c oxidase subunit I(cOI))and nuclear rhodopsin visual pigment(rho).Additionally,we inferred the molecular evolution of rho within the two contrasting environments(cave and surface)and across three geographic regions(Sierra de El Abra,Sierra de Guatemala,and Micos).In total,267 individuals were sequenced for the two mitochondrial fragments and 268 individuals were sequenced for the rho visual pigment from 22 cave and 46 surface *** results based on the mitochondrial data supported the two-lineage hypothesis,except for the Pachón and chica caves,whose introgression has been largely *** Sierra de El Abra region depicted the largest genetic diversity,followed by the Sierra de Guatemala *** the phylogeographic patterns of rho,we recovered exclusive haplogroups for the Sierra de El Abra(Haplogroup I)and Sierra de Guatemala regions(Haplogroup IV).Moreover,a 544 bp deletion in the rho gene was observed in the Escondido cave population from Sierra de Guatemala,reducing the protein from seven to three intramembrane *** change may produce a loss-of-function(LOF)but requires further *** nonsynonymous(dN)and synonymous(dS)substitution rates(omega valuesω),our results revealed

关键词： Visual pigments Selection cavefish Vision loss Loss of function

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Prevalence of metabolic syndrome, obesity and diabetes type 2 in cryptogenic cirrhosis

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World Journal of Gastroenterology 2008年第30期14卷 4771-4775页

作者： Felix I Tellez-Avila Francisco Sanchez-Avila mauricio García-Saenz-de-Sicilia Norberto c chavez-Tapia Ada M Franco-Guzman Gustavo Lopez-Arce Eduardo cerda-contreras Misael Uribe department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

AIM: To evaluate the prevalence of metabolic syndrome (MS), obesity and type 2 diabetes mellitus (T2DM) in a group of Mexican Mestizo patients with cryptogenic cirrhosis (cc) and to compare this group with patients with cirrhosis secondary to other causes (disease controls). METHODS: Patients with cc, diagnosed between January, 1990 and April, 2005, were included in a retrospective study. Patients with cirrhosis caused by chronic hepatitis c, alcohol abuse or autoimmune hepatitis (AIH) served as disease controls. RESULTS: A total of 134 patients with cc were analyzed. Disease controls consisted of 81 patients with chronic hepatitis c, 33 with alcohol abuse and 20 with AIH. The median age of patients with cc was 57 years (range, 16-87); 83 (61.9%) patients were female; 53 (39.6%) were child A, 65 (48.5%) child B, and 16 (11.9%) were child c cirrhosis. The prevalence of MS (29.1% vs 6%; P cc patients than in disease controls. There were no differences in sex, age or liver function tests between the two groups. cONcLUSION: The prevalence of MS, obesityand T2DM were higher in patients with cc than in patients with cirrhosis secondary to others causes. Our findings support the hypothesis that non-alcoholic steatohepatitis (NASH) plays an under-recognized role in cc.

关键词： cryptogenic chronic hepatitis Metabolic syndrome Obesity Diabetes mellitus

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