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Adenosine A1 receptor ligands bind toα-synuclein:implications forα-synuclein misfolding andα-synucleinopathy in Parkinson’s disease

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Translational Neurodegeneration 2022年第1期11卷 809-834页

作者： Elisabet Jakova Mohamed Taha Moutaoufik Jeremy s.lee Mohan Babu Francisco s.Cayabyab Department of surgery College of MedicineUniversity of SaskatchewanSaskatoonSKCanada Department of Chemistry and Biochemistry Faculty of ScienceUniversity of ReginaReginaSKCanada Department of Biochemistry Microbiology and ImmunologyCollege of MedicineUniversity of SaskatchewanSaskatoonSKCanada.

Background:Accumulatingα-synuclein(α-syn)aggregates in neurons and glial cells are the staples of many synucleinopathy disorders,such as Parkinson’s disease(PD).since brain adenosine becomes greatly elevated in ageing brains and chronic adenosine A1 receptor(A1R)stimulation leads to neurodegeneration,we determined whether adenosine or A1R receptor ligands mimic the action of known compounds that promoteα-syn aggregation(e.g.,the amphetamine analogue 2-aminoindan)or inhibitα-syn aggregation(e.g.,Rasagiline metabolite 1-aminoindan).In the present study,we determined whether adenosine,A1R receptor agonist N^(6)-Cyclopentyladenosine(CPA)and antago-nist 8-cyclopentyl-1,3-dipropylxanthine(DPCPX)could directly interact withα-syn to modulateα-syn aggregation and neurodegeneration of dopaminergic neurons in the substantia nigra(sN).Methods:Nanopore analysis and molecular docking were used to test the binding properties of CPA and DPCPX withα-syn in ***-Dawley rats were administered with 7-day intraperitoneal injections of the A1R ligands and 1-and 2-aminoindan,and levels ofα-syn aggregation and neurodegeneration were examined in the sN pars compacta and hippocampal regions using confocal imaging and Western ***:Using nanopore analysis,we showed that the A1R agonists(CPA and adenosine)interacted with the N-terminus ofα-syn,similar to 2-aminoindan,which is expected to promote a“knot”conformation andα-syn *** contrast,the A1R antagonist DPCPX interacted with the N-and C-termini ofα-syn,similar to 1-aminoindan,which is expected to promote a“loop”conformation that preventsα-syn *** docking studies revealed that adenosine,CPA and 2-aminoindan interacted with the hydrophobic core ofα-syn N-terminus,whereas DPCPX and 1-aminoindan showed direct binding to the N-and C-terminal hydrophobic *** imaging and Western blot analyses revealed that chronic treatments with CPA alone or in combination with 2-aminoindan increasedα-syn

关键词： Alpha-synucleinopathy Adenosine A1 receptor N6-cyclopentyladenosine 8-cyclopentyl-1 3-dipropylxanthine 1-aminoindan 2-aminoindan Neuroprotection Neurodegeneration Protein misfolding

LIGHTING QUALITY AND ACOUsTIC QUALITY IN leeD-CERTIFIED BUILDINGs UsING OCCUPANT EVALUATION

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Journal of Green Building 2011年第2期6卷 139-155页

作者： Young s.lee school of Planning Design&ConstructionMichigan State UniversityEast Lansing48823

Lighting quality and acoustic quality are often not well addressed in the current green building practice,including the Leadership in Energy and Environmental Design(leeD)Green Building Rating system in the *** the level of leeD certification indicates the level of sustainability,it is not clear if a higher level of leeD certification also implies a more comfortable and productive work *** study intended to find the relationship between the level of leeD certification and the level of worker satisfaction and perceived job performance regarding lighting quality and acoustic quality from fifteen leeD-certified *** findings indicate that the leeD Platinum building group tended to provide better lighting quality than the other lower certification groups,while the leeD Gold building group showed lower lighting quality and acoustic quality than the rest of the *** designers and organizations should be mindful of the importance of lighting and acoustic qualities in promoting better comfort and productivity as it is easy to overlook these criteria when complying with leeD IEQ guidelines.

关键词： lighting quality acoustics quality IEQ leeD

Potential annual daylighting performance of a high-efficiency daylight redirecting slat system

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Building simulation 2021年第3期14卷 495-510页

作者： Luis L.Fernandes Eleanor s.lee Anothai Thanachareonkit stephen E.selkowitz Lawrence Berkeley National Laboratory BerkeleyCaliforniaUSA

While the primary role of window attachments is often to moderate glare and solar heat gains,they are also able to provide additional daylight to interior *** this purpose,a variety of daylight-redirecting window systems have been developed over the past 150 *** reflective systems(slats/light shelves)or prismatic systems that rely on total internal reflection work well under specific solar conditions,but generally sacrifice performance over a much wider range of incident solar angles and sky *** systems-typically reflective slats-are more responsive to sun angles but have not been able to achieve optimal performance for glare and daylight redirection efficiency.A previous investigation into an adjustable,reflective blind concept first conceived of in the late 1970s showed promise but was not reduced to practice due to lack of adequate simulation and analysis *** this paper,this concept is further developed and its energy and visual comfort performance evaluated for four mid-latitude,temperate climates using ray-tracing simulation *** indicate significant potential lighting energy savings when compared with conventional automated reflective blinds(2.1-4.9 kWh/(m^(2)·a),or 14%-42%,depending on climate and orientation)or,especially,manually-operated matte white Venetian blinds(1.4-7.9 kWh/(m^(2)·a),or 9%-54%,depending on climate and orientation),while maintaining acceptable or better visual comfort conditions throughout the interior space.

关键词： daylighting windows light-redirecting systems dynamic fenestration

Mathematical modeling and simulations of stress mitigation by coating polycrystalline particles in lithium-ion batteries

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 947-962页

作者： N.IQBAL J.CHOI s.F.sHAH C.lee s.lee Department of Mechanical Roboticsand Energy EngineeringDongguk University-SeoulSeoul 04620Republic of Korea

A chemo-mechanical model is developed to investigate the effects on the stress development of the coating of polycrystalline Ni-rich LiNixMnyCo_(z)O_(2)(x≥0.8)(NMC)particles with poly(3,4-ethylenedioxythiophene)(PEDOT).The simulation results show that the coating of primary NMC particles significantly reduces the stress generation by efficiently accommodating the volume change associated with the lithium diffusion,and the coating layer plays roles both as a cushion against the volume change and a channel for the lithium transport,promoting the lithium distribution across the secondary particles more ***,the lower stiffness,higher ionic conductivity,and larger thickness of the coating layer improve the stress *** paper provides a mathematical framework for calculating the chemo-mechanical responses of anisotropic electrode materials and fundamental insights into how the coating of NMC active particles mitigates stress levels.

关键词： lithium-ion battery(LIB) polycrystalline particle coating finite element simulation Ni-rich LiNixMnyCo_(z)O_(2)(x>0.8)(NMC)

Perfluoroalkyl acid transformation and mitigation by nNiFe-activated carbon nanocomposites in steady-state flow column studies

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Journal of Environmental sciences 2023年第5期127卷 678-687页

作者： Mahsa Modiri-Gharehveran Younjeong Choi Jenny E.Zenobio Linda s.lee Department of Agronomy Purdue UniversityWest LafayetteIN 47907USA Interdisciplinary Ecological sciences&Engineering Purdue UniversityWest LafayetteIN 47907USA

The ongoing contamination of groundwater with per-and polyfluoroalkyl substances(PFAs)has resulted in a global and rapidly growing interest in PFAs groundwater *** technologies that lead to PFAs destruction are often limited by not addressing all PFAs,being energy-intensive or not being suited for in-situ *** developed nNiFeactivated carbon(AC)nanocomposites and demonstrated varying degrees of PFAs reduction and fluoride generation with these nanocomposites in batch reactors for several *** we explore nNiFe-AC’s effectiveness to transform perfluoroalkyl acid acids(PFAAs)under steady-state flow(0.0044 to 0.15 mL/min)in nNiFe-AC:sand packed *** experiments included,two perfluorooctane sulfonate(PFOs)in deionized water and two PFAA mixtures in deionized water or bicarbonate buffer containing five perfluoroalkyl carboxylates(PFCAs,C5-C9)and three perfluoroalkyl sulfonates(PFsAs,C4,C6 and C8)at temperatures of 50 or 60℃ were *** transformation was similar in PFOs-only and PFAA mixture column ***,%PFAA transformation under flow conditions exceeded what we observed previously in batch reactors with up to 53%transformation of a PFAA mixture with∼8%*** chain PFAs dominated the PFAAs transformed and a bicarbonate matrix appeared to reduce overall *** breakthrough was slower than predicted from only sorption due to transformation;some longer chain PFAs like PFOs did not ***,nNiFe-AC technology with both in-situ and ex-situ potential application was shown to be a plausible part of a treatment train needed to address the ongoing challenge for cleaning up PFAs-contaminated waters.

关键词： PFAAs PFAs Zero valent iron Nanoparticles Remediation Groundwater

A case study on multi-lane roundabouts under congestion:Comparing software capacity and delay estimates with field data

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Journal of Traffic and Transportation Engineering(English Edition) 2016年第2期3卷 154-165页

作者： Xuanwu Chen Ming s.lee Department of Civil and Environmental Engineering Florida International UniversityMiamiFL 33174USA Issaquah WA 98029USA

Existing studies on modern roundabouts performance are mostly based on data fron： singe lane roundabouts that are not heavily congested. For planners and designers interested in building multilane roundabouts for intersections with potential growth i~ future traffic, there has been a lack of existing studies with field data that provide reference values in terms of capacity and delay measurements. With the intent of providing such reference values, a case study was conducted by using the East DowlinC Road Roundabouts in Anchorage, Alaska, which are currently operating with extensive queues during the evening peak hours. This research used multiple video camcorders t（ capture vehicle turning movements at the roundabouts as well as the progressior~ of vehicle queues at the roundabout entrance approaches. With these video records, the number of vehicles in the queues can be accurately counted in any single minute during the peak hours. This study shows that unbalanced entrance flow patterns （i.e., ~ne entrance has significant higher flow than others） can intensify the queue and delay fo., the overall roundabouts. Then various software packages including RODEL, sIDRA and VIssIM were used to estimate several performance measurements, such as capacity. queue length, and delay, compared with the collected field data. With the comparison, it is found that all the three software packages overestimate multi-lane roundabout ca pacity before calibration. With default parameters, sIDRA and VIssIM tend to underes timate delays and queue lengths for the multi-lane roundabouts under congestion, while RODEL results in higher delay and queue length estimations at most of the entrance approaches.

关键词： Multi-lane roundabout Roundabout performance Capacity Delay Queue length Traffic simulation

Multiplexed electrospraying of water in cone-jet mode using a UV-embossed pyramidal micronozzle film

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Microsystems & Nanoengineering 2022年第5期8卷 247-256页

作者： Ji-hun Jeong Kwangseok Park Hyoungsoo Kim Inyong Park Jinyoung Choi seung s.lee Department of Mechanical Engineering Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Department of Environmental Machinery Korea Institute of Machinery and MaterialsDaejeon 34103Republic of Korea Department of Mechanical Engineering Dongshin UniversityNaju 58245Republic of Korea

The electrospraying of water in the cone-jet mode is difficult in practical applications owing to its low throughput and the electrical discharge caused by the high surface tension of water.A film with multiple dielectric micronozzles is essential for multiplexed electrospraying of water in cone-jet mode without electrical ***,a pyramidal micronozzle film with five nozzles was fabricated using the UV-embossing *** pyramidal micronozzle film consisted of pyramidal micronozzles,a micropillar array,and an in-plane extractor,which were proposed to minimize wetting and concentrate the electric field to the water meniscus at the tip of the pyramidal *** electrospraying of water using a single pyramidal micronozzle was visualized by a high-speed camera at a flow rate of 0.15–0.50ml/h with voltages of 0.0–2.3 kV,−1.6 kV,and−4.0 kV at the water,guide ring,and collector,*** distinct modes,the dripping,spindle,and cone-jet modes,were observed and distinguished according to the motion of the water meniscus at the nozzle *** steady Taylor cone and jet were observed in a voltage range of 1.3–2.0 kV in water,particularly in cone-jet *** electrospraying of water in cone-jet mode at a flow rate of 1.5ml/h was performed using a pyramidal micronozzle film,demonstrating the potential for a high-throughput electrospraying system.

关键词： process. film pyramidal

silk fibroin as an additive for cell-free protein synthesis

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synthetic and systems Biotechnology 2020年第3期5卷 145-154页

作者： Marilyn s.lee Chia-suei Hung Daniel A.Phillips Chelsea C.Buck Maneesh K.Gupta matthew W.Lux Us Army Combat Capabilities Development Command Chemical and Biological Center 8567 Ricketts Point RoadAberdeen Proving GroundMD21010USA Us Air Force Research Laboratory 217912th St.B652/R122 Wright-Patterson Air Force BaseOH45433USA Us Naval Research Laboratory Center for Bio/Molecular science and Engineering Bldg.42Room 3034555 Overlook Ave.WashingtonDC 20375UES Inc.4401 Dayton Xenia Rd.BeavercreekOH 45432USA

Cell-free systems contain many proteins and metabolites required for complex functions such as transcription and translation or multi-step metabolic *** into expanding the delivery of these systems by drying or by embedding into other materials is enabling new applications in sensing,point-of-need manufacturing,and responsive ***,silk fibroin from the silk worm,Bombyx mori,has received attention as a protective additive for dried enzyme formulations and as a material to build biocompatible hydrogels for controlled localization or delivery of biomolecular *** this work,we explore the effects of silk fibroin as an additive in cell-free protein synthesis(CFPs)*** of silk fibroin on CFPs activity and stability after drying,as well as the potential for incorporation of CFPs into hydrogels of crosslinked silk fibroin are *** find that simple addition of silk fibroin increased productivity of the CFPs reactions by up to 42%,which we attribute to macromolecular crowding ***,we did not find evidence that silk fibroin provides a protective effects after drying as previously described for purified ***,the enzymatic crosslinking transformations of silk fibroin typically used to form hydrogels are inhibited in the presence of the CFPs reaction *** attempts did not impact CFPs activity,but did yield localized protein aggregates rather than a *** discuss the mechanisms at play in these results and how the silk fibroin-CFPs system might be improved for the design of cell-free devices.

关键词： Cell-free systems Cell-free protein synthesis CFPs silk fibroin Preservation

Revisiting cerebral endothelial cells in Alzheimer’s disease

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Neural Regeneration Research 2025年第10期20卷 2911-2912页

作者： Amira s.Hanafy Department of Neurosurgery University Hospital BonnBonnGermany

Alzheimer’s disease(AD)is the most common neurodegenerative disorder characterized by slow and progressive decline of cognitive and memory *** only approximately 5%of the cases,AD is familial,as often predisposed by genetic mutations(Hoogmartens et al.,2021),while sporadic AD accounts for approximately 95%of the *** amyloid cascade hypothesis is one of the fundamental hypotheses put out to explain AD pathogenesis as dysregulated homeostasis of amyloid-β(Aβ)peptides that leads to the accumulation of Aβplaques in the parenchyma,an anatomical hallmark of AD.

关键词： Alzheimer Aβ cerebral