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Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

Journal of Bionic Engineering 2014年第3期11卷 346-359页

作者： Pedro miguel matos pereira Ana Sofia moita Gabriel Amaro monteiro Duarte miguel Franca Prazeres IBB - Institute for Biotechnology and Bioengineering Centre for Biological and Chemical Engineering Department of Bioengineering Instituto Superior Tecnico Universidade de Lisboa Av. Rovisco Pals 1049-001 Lisboa Portugal IN+ - Center for Innovation Technology and Policy Research Instituto Superior T~cnico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portugal

The topography and wettability of the underside of English weed （Oxalis pes-caprae） leaves and of their biomimetic replicas are investigated. Polyvinyl siloxane molds were cast from the leaves and then filled with an epoxy pre-polymer to produce replicas. The particular topographical structures of leaves and replicas were evaluated by optical microscopy and Scanning Electron microscopy （SEm） analysis. The static wettability of leaves and replicas was assessed by contact angle measurements, while the dynamic wettability was characterized by estimating contact angle hysteresis and studying the dynamic behavior of impacting water droplets. A smooth glass slip and its replica were used as control surfaces. The replica moulding method used was able to transfer the characteristic pattern of irregular 100 μm - 200 μm × 60 μm convex papillae interspersed with stomata of the original leaf to the epoxy replicas. The static contact angle of 143°± 3° and the contact angle hysteresis of 2~ indicate that the underside of the English weed leaf is close to superhydrophobic. The lower contact angles （130° ± 4°） and higher hysteresis （31°） observed for the replica when compared with the original leaves were associated to an inaccurate replication of the chemistry and structures of the three-dimensional wax projections covering the plant surface. Also, trichomes in the original leaves could not be accurately reproduced due to their flexibility and fragility. Differences in wetting behavior were also evident from droplet impact experiments, with rebound regimes prevailing in the original leaves and regimes characterized by higher adhesion and larger dissipation predominating in the replicas. Nevertheless, the morphological features of the leaf transferred to the replica were sufficient to promote a clear hydrophobic behavior of the replica when compared with the smooth epoxy reference surface.

关键词： biomimetic surfaces oxalis pes-caprae English weed replica molding wettability micro-topography

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Diet and microbiota in inflammatory bowel disease: The gut in disharmony

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World Journal of Gastroenterology 2017年第12期23卷 2124-2140页

作者： Davy Cm Rapozo Claudio Bernardazzi Heitor Siffert pereira de Souza Coordenacao de Pesquisa Instituto Nacional de Cancer Servico de Gastroenterologia e Laboratório multidisciplinar de Pesquisa Departamento de Clínica MédicaHospital Universitário Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro D’Or Institute for Research and Education Rua Diniz Cordeiro 30BotafogoRio de JaneiroRJ 22281-100Brazil

Bacterial colonization of the gut shapes both the local and the systemic immune response and is implicated in the modulation of immunity in both healthy and disease states. Recently, quantitative and qualitative changes in the composition of the gut microbiota have been detected in Crohn's disease and ulcerative colitis, reinforcing the hypothesis of dysbiosis as a relevant mechanism underlying inflammatory bowel disease(IBD) pathogenesis. Humans and microbes have coexisted and co-evolved for a long time in a mutually beneficial symbiotic association essential for maintaining homeostasis. However, the microbiome is dynamic, changing with age and in response to environmental modifications. Among such environmental factors, food and alimentary habits, progressively altered in modern societies, appear to be critical modulators of the microbiota, contributing to or co-participating in dysbiosis. In addition, food constituents such as micronutrients are important regulators of mucosal immunity, with direct or indirect effects on the gut microbiota. moreover, food constituents have recently been shown to modulate epigenetic mechanisms, which can result in increased risk for the development and progression of IBD. Therefore, it is likely that a better understanding of the role of different food components in intestinal homeostasis and the resident microbiota will be essential for unravelling the complex molecular basis of the epigenetic, genetic and environment interactions underlying IBD pathogenesis as well as for offering dietary interventions with minimal side effects.

关键词： Diet microbiota Epigenetics Crohn’s disease Ulcerative colitis

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Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

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Bio-Design and manufacturing 2019年第4期2卷 225-241页

作者： Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva morais Sílvia Vieira Hélder pereira Joao Espregueira-mendes Rui LReis Joaquim m.Oliveira B′s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 Barco GmrPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimaraesPortugal The Discoveries Centre for Regenerative and Precision medicine Headquarters at University of MinhoAvepark4805-017 BarcoGuimar'sPortugal Ripoll y De Prado Sports Clinic:murcia-madrid FIFA medical Centre of Excellence MurciaSpain Orthopedic Department Centro Hospitalar Póvoa de Varzim Vila do CondePortugal Clínica do Dragao Espregueira-Mendes Sports CentreFIFA Medical Centre of ExcellencePortoPortugal Dom Henrique Research Centre PortoPortugal Orthopedic Department University of MinhoBragaPortugal

The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 mPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 mPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 mPa and dynamical mechanical properties ranging from 1.03 ± 0.08 mPa to 1.56 ± 0.13 mPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hmCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie

关键词： Patient-specific Indirect printing Hierarchical Silk fibroin Enzymatic-cross-linking meniscus

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Improving wood volume predictions in dry tropical forest in the semi-arid Brazil

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Journal of Arid Land 2020年第6期12卷 1046-1055页

作者： Robson B de LImA Patrícia A B BARRETO-GARCIA Alessandro de PAULA Jhuly E S pereira Flávia F de CARVALHO Silvio H m GOmES Department of Forest Engineering State University of AmapáMacapá68900070Brazil Department of Forest Science State University of Southwest BahiaVitória da Conquista 45083900Brazil Universidade Federal de Lavras Lavras 37200900Brazil Universidade de São Paulo Piracicaba 13418900Brazil

The volumetric variability of dry tropical forests in Brazil and the scarcity of studies on the subject show the need for the development of techniques that make it possible to obtain adequate and accurate wood volume *** this study,we analyzed a database of thinning trees from a forest management plan in the Contendas de SincoráNational Forest,southwestern Bahia State,*** data set included a total of 300 trees with a trunk diameter ranging from 5 to 52 ***,validation and statistical selection of four volumetric models were *** to the difference in height values for the same diameter and the low correlation between both variables,we do not suggest models which only use the diameter at breast height(DBH)variable as a predictor because they accommodate the largest estimation *** comparing the best single entry model(Hohenald-Krenn)with the Spurr model(best fit model),it is noted that the exclusion of height as a predictor causes the values of 136.44 and 0.93 for Akaike information criterion(AIC)and adjusted determination coefficient(R2 adj),which are poorer than the second best model(Schumacher-Hall).Regarding the minimum sample size,errors in estimation(root mean square error(RmSE)and bias)of the best model decrease as the sample size increases,especially when a larger number of trees with DBH≥15.0 cm are randomly *** sampling by diameter class produces smaller volume prediction errors than random sampling,especially when considering all *** summary,the Spurr and Schumacher-Hall models perform *** models suggest that the total variance explained in the estimates is not less than 95%,producing reliable forecasts of the total volume with *** estimates indicate that the bias around the average is not greater than 7%.Our results support the decision to use regression methods to build models and estimate their parameters,seeking stratification strategies in diameter classes for the sample trees.

关键词： volume modeling minimal sample size Caatinga Spurr model forest management

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Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

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Green Chemical Engineering 2021年第4期2卷 412-422页

作者： Nathalia V.Veríssimo Carolina F.Saponi Timothy m.Ryan Tamar L.Greaves Jorge F.B.pereira Department of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical SciencesSao Paulo State University(UNESP)Rodovia Araraquara-Jaú/Km 0114800-903AraraquaraSPBrazil School of Science College of ScienceEngineering and HealthRMIT University124 La Trobe StreetMelbourneVIC 3000Australia School of Pharmaceutical Sciences Sao Paulo University(USP)Av.Prof.Lineu Prestesno.580B1605508-000Cidade de UniversitariaSao PauloSPBrazil Australian Synchrotron 800 Blackburn RoadClaytonVIC 3168Australia Univ Coimbra CIEPQPFDepartment of Chemical EngineeringRua Sílvio LimaPolo II–Pinhal de Marrocos3030-790 CoimbraPortugal

Fluorescent Proteins(FP)can be applied as biomarkers and biosensors in the industrial and medical fields,but their large-scale use,especially for new industrial applications,is limited due to their low ***,the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial *** this goal,we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based([C_(n)mim]Cl)ionic liquids(ILs)as additives to preserve the activity of Enhanced Green Fluorescent Protein(EGFP)at different storage times and under unfavorable ***[C_(n)mim]Cl solutions(at 0.100 mol L^(-1))were able to preserve EGFP fluorescence for longer than the phosphatesaline buffer(PBS)and NaCl solutions,increasing its fluorescence manifestation from 1 to 3 months.[C_(n)mim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence.[C_(n)mim]Cl also protected EGFP activity in the presence of the surfactant SDS,the acid guanidine hydrochloride,and H_(2)O_(2).Therefore,[C_(n)mim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on ***,there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions,and this is fundamental to enable expansion of FP in industrial and commercial applications.

关键词： Green Fluorescent Protein Protein stability Fluorescence Ionic liquids Preservatives

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Large ferro-pyro-phototronic effect in 0.5Ba(Zr_(0.2)Ti_(0.8))O_(3)-0.5(Ba_(0.7)Ca_(0.3))TiO_(3)thin films integrated on silicon for photodetection

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Carbon Energy 2023年第6期5卷 1-10页

作者： JoséP.B.Silva Katarzyna Gwozdz Luís S.marques mario pereira maria J.m.Gomes Judith L.macmanus‐Driscoll Robert L.Z.Hoye Centre of Physics of Universities of minho and Porto(CF‐Um‐UP) University of MinhoBragaPortugal Laboratory of Physics for materials and Emergent Technologies LapMETUniversity of MinhoBragaPortugal Department of Quantum Technologies Faculty of Fundamental Problems of TechnologyWroclaw University of Science and TechnologyWroclawPoland Department of materials Science and metallurgy CambridgeUK Inorganic Chemistry Laboratory Department of ChemistryUniversity of OxfordOxfordUK Department of materials Imperial College LondonLondonUK

Coupling together the ferroelectric,pyroelectric,and photovoltaic characteristics within a single material is a novel way to improve the performance of *** this work,we take advantage of the triple multifunctionality shown by 0.5Ba(Zr_(0.2)Ti_(0.8))O_(3)-0.5(Ba_(0.7)Ca_(0.3))TiO_(3)(BCZT),as demonstrated in an Al/Si/SiOx/BCZT/ITO thin-film *** Si/SiOx acts as an n-type layer to form a metal-ferroelectric-insulator-semiconductor heterostructure with the BCZT,and with Al and ITO as *** photo-response of the device,with excitation from a violet laser(405 nm wavelength),is carefully investigated,and it is shown that the photodetector performance is invariant with the chopper frequency owing to the pyro-phototronic effect,which corresponds to the coupling together of the pyroelectric and photovoltaic ***,the photodetector performance was significantly better than that of the devices operating based only on the pyro-phototronic effect by a factor of 4,due to the presence of ferroelectricity in the ***,after a poling voltage of−15 V,for a laser power density of 230mW/cm^(2)and at a chopper frequency of 400Hz,optimized responsivity,detectivity,and sensitivity values of 13.1mA/W,1.7×10^(10)Jones,and 26.9,respectively,are ***,ultrafast rise and fall times of 2.4 and 1.5μs,respectively,are obtained,which are 35,000 and 36,000 times faster rise and fall responses,respectively,than previous reports of devices with the ferro-pyro-phototronic *** is understood based on the much faster ferroelectric switching in ferroelectric thin films owing to the predominant 180°domains in a single direction out of plane.

关键词： ferroelectric ferro-pyro-phototronic effect photodetector polarization-controlled response

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Tandem running by foraging Pachycondyla striata workers in field conditions vary in response to food type,food distance,and environmental conditions

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Current Zoology 2021年第5期67卷 541-549页

作者： Janiele pereira SILVA Lohan VALADARES maria Eduarda Lima VIEIRA Serafino TESEO Nicolas CHALINE LEEEIS Departamento de Psicologia ExperimentalInstituto de Psicologia ExperimentalUniversidade de Sao PauloSao PauloSPCEP 05508-030Brazil Programa de pos-graduacao em Psicologia Experimental USPSao PauloSPCEP 05508-030Brazil Evolution GenomesBehavior and EcologyCNRS1 Avenue de la TerrasseGifsur-Yvette 91987France LBC Departamento de Fisiologia e ComportamentoCentro de BiocienciasUniversidade Federal do Rio Grande do NorteNatalRNCEP 59078-970Brazil School of Biological Sciences Nanyang Technological University60 Nanyang DriveSingapore 637551Singapore

Ants show collective and individual behavioral flexibility in their response to immediate context,choosing for example between different foraging *** Pachycondyla striata,workers can forage solitarily or recruit and guide nestmates to larger food sources through tandem *** considered more ancestral and less efficient than pheromone trail-laying,this strategy is common especially in species with small colony *** is not known is how the decision to recruit or follow varies according to the immediate *** is,how fine adjustments in information transfer affect immediate foraging decisions at the colony ***,we studied individually marked workers and evaluated their foraging decisions when food items varied in nature(protein versus carbohydrate),size,and distance from the nest at different temperatures and humidity *** results show that tandem run leaders and potential followers adjust their behavior according to a combination of external *** 84.2%of trips were solitary,most ants(81%)performed at least 1 tandem ***,tandem runs were more frequent for nearby resources and at higher relative ***,when food items were located far away,tandem runs were more successful when heading to protein sources(75%)compared with carbohydrate sources(42%).Our results suggest that the social information transfer between leaders and followers conveys more information than previously thought,and also relies on their experience and motivation.

关键词： collective behavior decision-making emotional states flexibility Ponerinae social learning

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Hydrodynamic Performance of Air-Filled Wave Attenuator for Wave Control:Experimental Study

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Journal of Shanghai Jiaotong university(Science) 2022年第3期27卷 316-325页

作者： pereira Eric Joseph TEH Hee-min mA Zhe Department of Civil and Environmental Engineering Universiti Teknologi PETRONASSeri Iskandar 32610PerakMalaysia Centre of Urban Resource Sustainability Institute of Self-Sustainable BuildingUniversiti Teknologi PETRONASSeri Iskandar 32610PerakMalaysia State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024LiaoningChina

Numerous types of floating breakwaters have been proposed,tested and commercialized in the past *** majority of these breakwaters are made of solid bodies;hence,they are relatively bulky and are not readily to be rapidly installed at the targeted sites when immediate wave protection of the coastal and offshore facilities is ***,the application of these hard floating structures at the recreational beaches is rather unlikely due to potential deadly marine traffic *** overcome these problems,a flexible air-filled wave attenuator(AFWA)has been developed in the present *** floating breakwater is made of flexible waterproof membrane *** main body consists of a rectangular air-filled prism and is ballasted by sandbags located around the floating *** objective of this study is to evaluate the wave transmission,wave reflection,energy dissipation,motion responses and mooring forces of the AFWA under the random wave actions using physical *** test model located in a 20 m long wave flume was subjected to a range of wave heights and *** wave profiles in the vicinity of the test model were measured using wave probes for determination of wave transmission,reflection and energy loss *** motion responses in terms of heave,surge and pitch,and wave forces acting on the mooring lines were measured using a motion tracking system and load cells,*** experimental results reveal that the AFWA is effective in attenuating up to 95%in the incoming wave height and has low-wave-reflection properties,which is commendable for floating breakwaters.

关键词： floating breakwater air-filled wave attenuator(AFWA) hydrodynamic performance wave attenuation motion responses mooring forces

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Seaweeds’nutraceutical and biomedical potential in cancer therapy:a concise review

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Journal of Cancer metastasis and Treatment 2021年第1期7卷 189-212页

作者： João Cotas Diana Pacheco Ana m.m.Gonçalves Paulo Silva Loïc G.Carvalho Leonel pereira University of Coimbra MARE-Marine and Environmental Sciences CentreDepartment of Life SciencesCalçada Martim de FreitasCoimbra 3000-456Portugal Department of Biology and CESAm University of AveiroAveiro 3810-193Portugal UCIBIO-REQUImTE Departamento de QuímicaFaculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparica 2829-516Portugal The James Hutton Institute Dundee DD25DAScotlandUK Heriot-Watt University Edinburgh EH144ASScotlandUK.

Seaweeds have been a food source since ancient times(600 BC)and are still widely used in Asia,mainly in traditional Chinese medicine and Japanese folk ***,seaweed compounds and extracts have been gaining interest from the biomedical and pharmaceutical market *** have been referenced as feasible solutions in finding new potential compounds and therapies in prevention,control,and reduction of cancer development due to the multirole of some bioactive components(e.g,phenolic compounds and sulphated polysaccharides).moreover,seaweeds are rich in important health-promoting molecules[such as poly and highly unsaturated fatty acids(PUFAs and HUFAs),essential amino acids,vitamins,and dietary fibers]and minerals(calcium,iron,iodine,magnesium,phosphorus,potassium,zinc,copper,manganese,selenium,and fluoride).In this review,the potential therapeutic effects of seaweed in the prevention and treatment of cancer are approached,as well as nutraceutical properties of seaweed to promote cell homeostasis.

关键词： Seaweed compounds nutraceutical bioactive molecules cancer prevention cancer treatment

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Solar spectral conversion based on plastic films of lanthanide-doped ionosilicas for photovoltaics:Down-shifting layers and luminescent solar concentrators

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Journal of Rare Earths 2020年第5期38卷 531-538页

作者： m.A.Cardoso S.F.H.Correia A.R.Frias H.m.R.Goncalves R.F.P.pereira S.C.Nunes m.Armand P.S.André V.de Zea Bermudez R.A.S.Ferreira Department of Physics and CICECO-Aveiro Institute of materials University of Aveiro3810-193AveiroPortugal Department of Chemistry and CQ-VR University of Tras-os-Montes e Alto Douro5000-801Vila RealPortugal Instituto de Telecomunicacoes University of Aveiro3810-193AveiroPortugal REQUImTE Instituto Superior de Engenharia do Porto4200-072PortoPortugal Chemistry Centre and Chemistry Department University of Minho4710-057BragaPortugal CICS-Health Sciences Research Center and Chemistry Department University of Beira Interior6201-001CovilhaPortugal Electrical Energy Storage Department CIC EnergiguneParque Tecnologico de AlavaMinano01510Spain Department of Electrical and Computer Engineering Instituto de TelecomunicacoesInstituto Superior TecnicoUniversidade de Lisboa1049-001LisbonPortugal

The mismatch between the photovoltaic(PV)cells absorption and the solar irradiance on earth is one of the major limitations towards more efficient PV energy *** aspect was addressed by downshifting the solar irradiance on Earth through luminescent down-shifting layers based on lanthanidedoped surface-functionalized ionosilicas(ISs)embedded in poly(methyl methacrylate)(PmmA)coated on the surface of commercial Si-based PV *** IS-PmmA hybrid materials exhibit efficient solar radiation harvesting(spectral overlap of^9.5×10^19 photons/(s·m2))and conversion(quantum yield^52%).The direct solar radiation and the down-shifted radiation are partially guided and lost through total internal reflection to the layer edges being unavailable for PV conversion of the coated PV *** tuning the down-shifting layer thickness,it also acts as luminescent solar concentrator enabling the collection of the guided radiation by flexible PV cells applied on the borders of the down-shifting layer leading to an enhancement of the PV energy conversion from^5%(in the case of the single-use of the luminescent down-shifting layer)to^13%comparing with the bare PV *** overall electrical output of the device resulted in an absolute external quantum efficiency increase of^32%for the optimized Eu^3+-based films in the UV spectral region(compared with the bare PV device,which is among the best values reported so far).

关键词： Ionosilicas Lanthanides Photovoltaics Luminescent solar concentrators Down-shifting layers Silicon photovoltaic cells

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