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Comparative Transcriptomic Analysis of Two Tomato Cultivars with Different Shelf-Life Traits

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Phyton-International Journal of Experimental Botany 2024年第8期93卷 2075-2093页

作者： Abdul karim Amin Yan He Xianglong Wang Pengwei Li muhammad Ahmad Hassan Mohammad Yousof Soltani Yiling Zhang Mohammad Alem Amin Ahmad Shah Ahmadzai Yajing Liu Songhu Wang Anhui Province Key Laboratory of Horticultural Crop Quality Biology School of HorticultureAnhui Agricultural UniversityHefei230036China Anhui Academy of Agricultural Sciences Hefei230001China Anhui Province Key Laboratory of Soybean Molecular Breeding and Crop Quality Biology School of AgronomyAnhui Agricultural UniversityHefei230036China Department of Horticulture Ghazni UniversityGhazni2301Afghanistan State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing210095China

Tomato(Solanum lycopersicum)is a perishable fruit because of its fast water loss and susceptibility to pathogens in the post-harvest stage,which leads to huge economic losses every *** this study,firstly from 19 tomato cultivars,we screened out two cultivars,Riogrand and SalarF1,having long and short shelf-life spans,***,shelf-life analysis was carried out for both cultivars at room *** exhibited that Riogrand showed higher firmness and less weight loss than *** ethylene production was higher in SalarF1,compared with Riogrand during post-harvest *** performed transcriptomic analysis of both cultivars in different storage *** discovered 2913,2188,and 11,119 differentially expressed genes(DEGs)for three post-harvest stages(0,20,and 40 Days Post-Harvest(DPH)),*** genes are enriched in ethylene biosynthesis and response,as well as cell wall-related *** response factor(ERF)ERF2 and ERF4 were highly expressed in SalarF1 with a short shelf life in 40 DPH,and the ethylene biosynthetic genes ACO1,ACO4,ACS6,and ACS2 were significantly upregulated in *** cell wall loosening and cell wall-related genes XTH3,XTH7,XTH23,1,3;1,4-β-D-Gluc-like,pGlcT1,Cellulase,PGH1,PL5,PL-like 1,PL-like 2 exhibited the highest levels of significance,being notably upregulated in the last stage of *** quantitative real-time polymerase chain reaction(qRT-PCR)analysis validated these gene expressions,which is in line with the transcriptome *** findings suggested that the extension of tomato fruit shelf life is mostly dependent on ethylene biosynthesis,signaling pathway genes,cell wall loosening,and cell wall-associated genes.

关键词： Shelf life transcriptome analysis tomato differentially expressed genes(DEGs) ethylene

National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan Ahmed muhammad Atique Usman Iqbal Aujla muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt Abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib Haider Siraj Haider Syed Mujahid Hassan Adnan Abdul Jabbar Aman Nawaz Khan muhammad Shoaib Khan muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad karim Malik Kamran Rashid Sohail Rashid Mohammad Salih Abdullah Saeed Amjad Salamat Ghias-un-Nabi Tayyab Aasim Yusuf Haseeb Haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

Fungicide Resistance in Colletotrichum fructicola and Colletotrichum siamense Causing Peach Anthracnose in China

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Fungicide Resistance in Colletotrichum fructicola and Collet...

New Developments on Operator Reverse AM-GM Inequality for Positive Linear Maps

中国植物病理学会2024年学术年会

作者： Mohammad Mazharul karim Hafiz muhammad Usman TAN Qin HU Jiajie FAN Fei Rafakat Hussain LUO Chaoxi National Key Laboratory for Germplasm Innovation Utilization of Horticultural Crops Huazhong Agricultural University Department of Plant Pathology College of AgricultureGuizhou University Hubei Key Lab of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural University

Peach is one of the popular and economically important fruit crops in *** cultivation is hampered due to attacks of anthracnose disease,causing significant economic *** fructicola and Colletotrichum siamense belong to the Colletotrichum gloeosporioides species complex and are considered major pathogens of peach *** of different groups of fungicides is a routine approach for controlling this ***,fungicide resistance is a significant drawback in managing peach anthracnose *** this study,39 isolates of *** and 41 isolates of *** were collected from different locations in various provinces in *** sensitivity of *** and *** to some commonly used fungicides,i.e.,carbendazim,iprodione,fluopyram,and propiconazole,was *** the isolates of *** collected from Guangdong province showed high resistance to carbendazim,whereas isolates collected from Guizhou province were *** ***,isolates collected from Hebei province showed moderate resistance,while those from Shandong province were sensitive to *** the other hand,all the isolates of *** and *** showed high resistance to the dicarboximide(DCF) fungicide iprodione and succinate dehydrogenase inhibitor(SDHI) fungicide fluopyram,indicating natural resistance in these species to these ***,they are all sensitive to the demethylation inhibitor(DMI) fungicide *** cross-resistance was observed between carbendazim and benomyl as they are members of the same methyl benzimidazole carbamate(MBC) *** no correlation of sensitivity was observed between different groups of *** significant differences were found in each fitness parameter between carbendazim-resistant and sensitive isolates in both *** characterization of the β-tubulin 2(TUB2) gene revealed that in ***,the E198 A point mutation was the determin

关键词： Fungicide Resistance in Colletotrichum fructicola and Colletotrichum siamense Causing Peach Anthracnose in China

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Acta Mathematica Sinica,English Series 2022年第2期38卷 419-430页

作者： Shazia karim Ilyas ALI muhammad MUSHTAQ Department of Mathematics University of Engineering and Technology LahorePakistan Department of Basic Sciences and Humanities University of Engineering and Technology LahoreFaisalabad CampusPakistan

In this research article,we shall give some reverse Arithmetic-Geometric mean inequalities for unital positive linear maps on Hilbert space operators under some different *** results are sharper and more precise as co... 详细信息

关键词： Positive operators unital positive linear maps operator AM-GM inequality operator reverse AM-GM inequality operator norm

Real-Time Safety Helmet Detection Using Yolov5 at Construction Sites

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Intelligent Automation & Soft Computing 2023年第4期36卷 911-927页

作者： Kisaezehra muhammad Umer Farooq muhammad Aslam Bhutto Abdul karim Kazi Department of Computer Science and Information Technology NED University of Engineering&TechnologyKarachi75270Pakistan Department of Civil Engineering NED University of Engineering&TechnologyKarachi75270Pakistan

The construction industry has always remained the economic and social backbone of any country in the world where occupational health and safety(OHS)is of prime *** in other developing countries,this industry pays very little,rather negligible attention to OHS practices in Pakistan,resulting in the occurrence of a wide variety of accidents,mishaps,and near-misses every *** of the major causes of such mishaps is the non-wearing of safety helmets(hard hats)at construction sites where falling objects from a height are *** most cases,this leads to serious brain injuries in people present at the site in general and the workers in *** is one of the leading causes of human fatalities at construction *** the United States,the Occupational Safety and Health Administration(OSHA)requires construction companies through safety laws to ensure the use of well-deﬁned personal protective equipment(PPE).It has long been a problem to ensure the use of PPE because round-the-clock human monitoring is not ***,such monitoring through technological aids or automated tools is very much *** present study describes a systema-tic strategy based on deep learning(DL)models built on the You-Only-Look-Once(YOLOV5)architecture that could be used for monitoring workers’hard hats in *** can indicate whether a worker is wearing a hat or *** proposed system usesﬁve different models of the YOLOV5,namely YOLOV5n,YOLOv5s,YOLOv5 m,YOLOv5l,and YOLOv5x for object detection with the support of PyTorch,involving 7063 *** results of the study show that among the DL models,the YOLOV5x has a high performance of 95.8%in terms of the mAP,while the YOLOV5n has the fastest detection speed of 70.4 frames per second(FPS).The proposed model can be successfully used in practice to recognize the hard hat worn by a worker.

关键词： Object detection computer-vision personal protective equipment(PPE) deep learning industry revolution(IR)4.0 safety helmet detection

Bacterial endotoxins in periodontal health and diseases

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BIOCELL 2023年第1期47卷 81-89页

作者： FARIHA NUSRAT MOHAMMAD TARIQUR RAHMAN muhammad MANJURUL karim Department of Mathematics and Natural Sciences BRAC UniversityDhaka1212Bangladesh Faculty of Dentistry University of MalayaKuala Lumpur50603Malaysia Department of Microbiology University of DhakaDhaka1000Bangladesh

Bacterial endotoxins are a major concern in periodontal health and diseases owing to their structure and biological *** up-to-date knowledge of endotoxins and the recent findings about the influence of endotoxins in dental health,their probable mode of pathogenesis,and standard detection methods,this review analyzes the potential efficacy and benefits of probiotics in combination with conventional and contemporary treatment *** the oral cavity,Gram-negative bacteria are documented to predominate in the pulpal lesions with radiolucent areas and in the root canal with pulp necrosis,where they pose an absolute threat by promoting a series of inflammatory ***,a constituent of Gram-negative bacteria establishes a nexus between cytokine stimulation and proinflammatory reactions,therefore plays a critical role in decaying dental pulp and modulating periodontal ***,the treatment regimen involves several biochemical *** addition,probiotics have been reported to control endotoxin in gingivitis and contribute to the overall improvement of dental health.A potential benefit of a combination of probiotics as a complementary treatment along with the conventional treatment warrant more empirical evidence to elucidate its role and mechanism in resolving the clinical manifestations associated with endotoxins in the periodontal region.

关键词： Dental health LPS Oral probiotics Periodontitis

Push-Based Content Dissemination and Machine Learning-Oriented Illusion Attack Detection in Vehicular Named Data Networking

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Computers, Materials & Continua 2023年第9期76卷 3131-3150页

作者： Arif Hussain Magsi Ghulam muhammad Sajida karim Saifullah Memon Zulfiqar Ali State Key Laboratory ofNetworking and Switching Technology Beijing University of Post and TelecommunicationBeijing100876China Department of Computer Engineering College of Computer and Information SciencesKing Saud UniversityRiyadh11543Saudi Arabia School of Computer Science and Technology Harbin Institute of TechnologyHarbin150001China School of Computer Science and Electronic Engineering University of EssexColchesterCO43SQUK

Recent advancements in the Vehicular Ad-hoc Network(VANET)have tremendously addressed road-related ***,Named Data Networking(NDN)in VANET has emerged as a vital technology due to its outstanding ***,the NDN communication framework fails to address two important *** current NDN employs a pull-based content retrieval network,which is inefficient in disseminating crucial content in Vehicular Named Data Networking(VNDN).Additionally,VNDN is vulnerable to illusion attackers due to the administrative-less network of autonomous *** various solutions have been proposed for detecting vehicles’behavior,they inadequately addressed the challenges specific to *** deal with these two issues,we propose a novel push-based crucial content dissemination scheme that extends the scope of VNDN from pullbased content retrieval to a push-based content forwarding *** addition,we exploitMachine Learning(ML)techniques within VNDN to detect the behavior of vehicles and classify them as attackers or *** trained and tested our system on the publicly accessible dataset Vehicular Reference Misbehavior(VeReMi).We employed fiveML classification algorithms and constructed the bestmodel for illusion attack *** results indicate that RandomForest(RF)achieved excellent accuracy in detecting all illusion attack types in VeReMi,with an accuracy rate of 100%for type 1 and type 2,96%for type 4 and type 16,and 95%for type ***,RF can effectively evaluate the behavior of vehicles and identify attacker vehicles with high *** ultimate goal of our research is to improve content exchange and ***,ourML-based attack detection and preventionmechanismensures trustworthy content dissemination and prevents attacker vehicles from sharing misleading information in VNDN.

关键词： Named data networking vehicular networks pull-push illusion attack machine learning

A survey of machine learning techniques for detecting and diagnosing COVID-19 from imaging

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Quantitative Biology 2022年第2期10卷 188-207页

作者： Aishwarza Panday muhammad Ashad Kabir Nihad karim Chowdhury Department of Computer Science&Engineering Stamford UniversityDhaka 1217Bangladesh School of Computing and Mathematics Charles Sturt UniversityNSW 2795Australia Department of Computer Science&Engineering University of ChittagongChittagong 4349Bangladesh

Background: Due to the limited availability and high cost of the reverse transcription-polymerase chain reaction (RT- PCR) test, many studies have proposed machine learning techniques for detecting COVID-19 from medical imaging. The purpose of this study is to systematically review, assess and synthesize research articles that have used different machine learning techniques to detect and diagnose COVID-19 from chest X-ray and CT scan ***: A structured literature search was conducted in the relevant bibliographic databases to ensure that the survey solely centered on reproducible and high-quality research. We selected papers based on our inclusion ***: In this survey, we reviewed 98 articles that fulfilled our inclusion criteria. We have surveyed a complete pipeline of chest imaging analysis techniques related to COVID-19, including data collection, pre-processing, feature extraction, classification, and visualization. We have considered CT scans and X-rays as both are widely used to describe the latest developments in medical imaging to detect ***: This survey provides researchers with valuable insights into different machine learning techniques and their performance in the detection and diagnosis of COVID-19 from chest imaging. At the end, the challenges and limitations in detecting COVID-19 using machine learning techniques and the future direction of research are discussed.

关键词： COVID-19 machine learning deep learning detection classification diagnosing X-ray CT scan

A review on promising phytochemical, nutritional and glycemic control studies on Moringa oleifera Lam. in tropical and sub-tropical regions

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Asian Pacific Journal of Tropical Biomedicine 2016年第10期6卷 896-902页

作者： Hafiz Irfan muhammad Mohd Zaini Asmawi Nurzalina Abdul karim Khan Discipline of Pharmacology School of Pharmaceutical Sciences Universiti Sains Malaysia Department of Pharmacology Faculty of Pharmacy University of Sargodha Discipline of Pharmaceutical Technology School of Pharmaceutical Sciences Universiti Sains Malaysia

Plants have provided sources to find novel compounds. These plants are being used as therapeutic purposes since the birth of mankind. The traditional healers normally utilize medicinal plants as crude drugs while scientists using the folk claim as guides to explore medicinal plants. Moringa oleifera is a famous edible plant having therapeutic and nutritive values. The present study was designed to cumulate the research data regarding to what extent, phytochemical, nutritional and glycemic control studies has been explored using its different extracts. The articles indicated that the powder, aqueous, methanol and ethanol extracts of Moringa oleifera(leaves, pods, seeds, stem and root bark) have significant therapeutic herbal potential to treat diabetes mellitus. Collectively, the mechanism behind is intestinal glucose inhibition, insulin release as well as decrease in insulin resistance probably regeneration of b-cells of pancreas, increase in glutathione and reduction in malondialdehyde. Conclusively, this article give descriptive information about antidiabetic effect, claimed marker compounds and proposed antihyperglycemic mechanism of a single plant. It can be suggested a potential herbal source to treat diabetes mellitus as being widely accepted by major population as nutrition and therapeutic agent.

关键词： Antihyperglycemic Aqueous Ethanol Moringa oleifera Antidiabetic