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Human Embryo Neuronal Culture <i>in Vitro</i>: A Model to Study Cellular Physiology, Receptors, Power and Toxicity of Cytostatic Drugs for Human Use

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Neuroscience & Medicine 2012年第3期3卷 321-326页

作者： Stabile Mariano Monaco Roberto Iuorio Tina Buoninfante Luca Marino Lucia Altieri Vincenzo Della Ragione Carlo masillo francesco Anatomy Pathology Department Hospital “A. Cardarelli” Naples Italy Department of Gynecology and Obstetrics Hospital “Maria SS Addolorata” Eboli Italy Genetic Department Hospital “Ele-nad’ Aosta” Naples Italy ZigoteGenetic and Prenatal Diagnosis Centre Salerno Italy

Neural cells cultures from human embryo brain of 9° - 11°W gestational age have been used to study ERα (Estrogens Receptor α) and to perform toxicity test for Mitomycin C and Methotrexate. Histochemical confirmation of cellular neuronal phenotype was based on histochemical evidence of NSE (Neuron Specific Enolase).The detection of ERα in neuronal cells was performed with a rabbit Monoclonal Antibody. ERα was absent both on neurons grown in vitro and on tissue brain specimens. This finding is apparently in contrast with the positive immunoreactivity of ERα and ERβ reported by other Authors on foetal and adult CNS (Central Nervous System). The absence of nuclear ERα on neurons in culture and in brain tissue specimens in our experiment is not in contrast with the relevant physiologic role of estrogens on nervous central system, but it could be correlated to the embryonic period of life and could represent a protection of male brain from an undue estrogens imprinting. The mitomycin C, alkylation agent, has shown in our experiment a major neurotoxic and cytostatic power in comparison with methotrexate. Our conclusion is that human embryo neuronal culture in vitro is a powerful instrument for physiology and human therapy for cancer and neurodegenerative diseases.

关键词： Human Embryo Neuronal Culture ERα on Embryonic Brain Mitomycin C Toxicity Test in Vitro Methotrexate Toxicity Test in Vitro

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