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Regenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces

Chinese Science Bulletin 2013年第35期58卷 4349-4356页

作者： UMEMOTO Terumasa YAMATO masayuki NISHIDA Kohji OKANO Teruo Institute of Advanced Biomedical Engineering and Science Tokyo Women’s Medical University Tokyo 162-8666 Japan Department of Ophthalmology Osaka University Medical School Osaka 565-0871 Japan

Recently,regenerative medicine has been focused on as next-generation definitive therapies for several diseases or injuries for which there are currently no effective *** therapies have been rapidly developed through the effective fusion between different fields such as stem cell biology and *** far,we have proposed"cell sheet engineering"through our core technology that simply applies alterations of the temperature which allows regulation of the attachment or detachment of living cells on the culture surfaces grafted with the temperature-responsive polymer"poly(N-isoproplyacrylamide)".This technology enables us to construct bioengineered sheet-like tissues,termed"cell sheets",without the need of using biodegradable scaffolds and protease treatments that are traditionally ***,our carrier-free cell sheets not only are independent of the issues observed in conventional methods,but also showed further advantages in the reconstruction of the corneal epithelium or endothelium(*** of optical transparency and cell-cell interactions to host stroma in reconstructed tissues).Moreover,our approach does not have issues such as immune rejection or limited number of donors,due to the use of cell sheets derived from autologous limbal(in patients with unilateral disease)or oral mucosal epithelial cells(in patients with bilateral disorders).Indeed,we have successfully performed the clinical application for the reconstruction of ocular surfaces through the transplantation of our carrier-free corneal epithelial cell sheets,as evidenced by improved visual acuity as well as long-term maintenance of postoperative health conditions on ocular surfaces in all *** have also proposed a novel approach for the reconstruction of the corneal endothelium using corneal endothelial cell sheets by demonstrating its successful ***,our cell sheet engineering technique provides a breakthrough in the field of regenerative medicine appli

关键词：干细胞生物学角膜上皮表面重建再生医学调节温度工程化反应性角膜内皮细胞

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Lattice Boltzmann Flow Simulation in a Combined Nanochannel

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Advances in Applied Mathematics and Mechanics 2010年第5期2卷 609-625页

作者： Kazuhiko Suga Susumu Takenaka Takahiko Ito masayuki Kaneda Department of Mechanical Engineering Osaka Prefecture UniversitySakai 599-8531Japan

The widely used micro-flow wall-boundary conditions for lattice Boltzmann method(LBM)are evaluated in a force driven combined nanochannel *** flow field consists of a two-dimensional nanochannel(mother channel)of an infinite length having flat plates of a finite length *** flat plate is set above the bottom wall of the nanochannel with a narrow *** flow,thus,develops through this narrow gap(narrower channel)and the other side of the plate(wide gap).The Knudsen number based on the mother channel height is Kn=0.14 whereas the characteristic Knudsen number in the narrower channel is *** obtain the reference data,the molecular dynamics(MD)simulation is performed with a fully diffusive wall *** LBMs are based on the lattice BGK model and with the bounce-back/specular reflection(BSBC)and the diffuse scattering(DSBC)wall boundary *** relaxation time is modified to include sensitivity to *** DSBC shows generally satisfactory results in the test flow cases including fully developed force driven Poiseuille flows,where the BSBC performs worse at Kn>0.5 with a fixed bridge coefficient of b=*** results in its overprediction of the flow rate in the narrower channel region since the characteristic Knudsen number there is *** MD simulation suggests that the flow develops gradually through the narrower channel region though all the LBM predictions show almost instant flow *** fact suggests that the relaxation time model needs to have more sensitivity to the locally defined *** discussions of the BSBC with a different set of models suggest that the regularization process is required for predicting complex nanoscale flows.

关键词： Lattice Boltzmann method Knudsen number molecular dynamics simulation nanochannel wall boundary condition slippage velocity

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Vascular remodeling and mobilization of bone marrow-derived cells in cuff-induced vascular injury in LDL receptor knockout mice

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Chinese Medical Journal 2008年第3期121卷 220-226页

作者： XU Yang ARAI Hidenori MURAYAMA Toshinori KITA Toru YOKODE masayuki Institute of Medicinal Plant Development Chinese Academy ofMedical Sciences and Peking Union Medical College Beijing100094 China Department of Clinical Innovative Medicine Kyoto University Graduate School of Medicine 54 Kawahara-cho Shogoin Sakyo-ku Kyoto 606-8507 Japan Department of Geriatric Medicine Kyoto University Graduate School of Medicine 54 Kawahara-cho Shogoin Sakyo-ku Kyoto 606-8507 Japan Department of Cardiovascular Medicine Kyoto University Graduate School of Medicine 54 Kawahara-cho Shogoin Sakyo-ku Kyoto 606-8507 Japan Translational Research Center Kyoto University Graduate School of Medicine 54 Kawahara-cho Shogoin Sakyo-ku Kyoto 606-8507 Japan

Background Vascular remodeling is an important pathologic process in vascular injury for various vascular disorders such as atherosclerosis, postangioplasty restenosis and transplant arteriopathy. Recently, pathologic change and the role of bone marrow derived cells were wildly studied in atherosclerosis and restenosis. But the manner of lesion formation in neointima and cell recruitment in vascular remodeling lesion in the present of hypercholesterolemia is not yet fully understood. Methods Double-transgenic mice knockout of LDL receptor gene （LDL-/-） and expressing ubiquitously green fluorescent protein （GFP） were obtained by cross-breeding LDL -/- mice with the GFP-expressing transgenic mice. LDL-/- mice （22-24 weeks of age） fed high fat diet containing 1.25% （w/w） cholesterol were subjected to 9Gy irradiation and received bone marrow （BM） cells from the double-transgenic mice. Four weeks later, a nonconstrictive cuff was placed around the right femoral artery. After another 2 weeks, both right and left femoral arteries were harvested and subjected to histochemical analysis. Apoptosis was analyzed in situ using TUNEL assay. Results Two weeks after cuff placement, atherosclerotic lesions developed in the intima consisting of a massive accumulation of foam cells. The tissue stained with anti-α smooth muscle actin （SMA） antibody, showed a number of SMA-positive cells in the intimal lesion area. They were also positive for GFP, indicating that BM-derived cells can differentiate to SMCs in the intima in cuff-induced vascular remodeling lesions. Numerous small vessels in the adventitia as well as the endothelial lining of the intima were positive both for CD31 and GFP. The intima and media showed a large number of TUNEL-positive signals after 2 weeks cuff injury, indicating the presence of apoptosis in vascular remodeling. Conclusions Atherosclerotic lesions in mice can be developed in the intima after 2 weeks of cuff-induced vascular injury under the hypercholes

关键词： vascular remodeling macrophage smooth muscle cell endothelial cell bone marrow

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Electrochemical Characterization of a Porous Pt Nanoparticle ＂Nanocube-Mosaic＂ Prepared by a Modified Polyol Method with HCI Addition

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Nano Research 2011年第8期4卷 746-758页

作者： Randy Jalem Ryosuke Koike Yong Yang Masanobu Nakayama masayuki Nogami Department of Materials Science and Engineering Nagoya Institute of Technology Showa Nagoya 466-8555 Japan

Porous and single crystalline platinum （Pt） nanoparticles （NPs） have been successfully synthesized by reduction of H2PtC16.6H20 and then investigated by optical spectroscopy and transmission electron microscopy. H2PtCI6-6H20 was reduced using ethylene glycol in the presence of polyvinylpyrrolidone under highly acidic conditions （pH 〈 1） to form single crystalline Pt particles about 5 nm in size. These particles were then stacked via {100} facets, forming 50-nm length porous nanocubes with a mosaic structure. The porous Pt NPs exhibited excellent catalytic properties for methanol oxidation. In particular, the electrochemical surface area was -63 m2/g, five times higher than that for non-porous Pt NPs prepared using a conventional method. We suggest that the high catalytic activity of porous Pt NPs is due to a combination of the crystalline structure having exposed {100} facets and a porous morphology.

关键词： Platinum porous nanoparticles catalyst methanol oxidation cyclic voltammetry

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Interaction between Cd and Pb in the soil-plant system: a case study of an arid oasis soil-cole system

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Journal of Arid Land 2014年第1期6卷 59-68页

作者： ZhuanJun ZHAO ZhongRen NAN ZhaoWei WANG YiMing YANG masayuki SHIMIZU College of Resource & Environment Lanzhou University Department of Soil and Crop Science Colorado State University

The Hexi Corridor,our study area,is located in Northwest China and is also the most developed area of oasis farming in arid regions of Northwestern ***,the rapid development of metallurgy and chemical industries in this region poses a great threat to the accumulation of heavy metals in *** objectives of this study are(1)to determine the influence of heavy metals on plant growth;(2)to assess the translocation capability of heavy metals in soil-plant system;and(3)to investigate the interaction between heavy *** experiments were conducted on cole(Brassica campestris L.)grown in the arid oasis soils singly and jointly treated with cadmium(Cd)and lead(Pb).Nine treatments were applied into the *** the same planting conditions,three scenarios of Cd,Pb and Cd–Pb were designed to compare the interaction between Cd and *** results showed that the response of cole weights to Cd,Pb and Cd–Pb treatments was slight,while Cd and Pb uptakes in cole were more sensitive to the single effects of Cd and Pb concentration in soils from the lower treatment *** the influence of the single Cd,Pb and joint Cd–Pb treatments,Cd concentrations were lower in the cole roots than in the shoots,while for Pb,the results were *** studies revealed that the interaction of Cd and Pb could weaken the cole’s ability to uptake,concentrate and translocate heavy metals in arid oasis soils.

关键词： heavy metals interaction vegetables oasis soils arid regions Brassica campestris L.

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Risk factors and diagnostic biomarkers for nonalcoholic fatty liver disease-associated hepatocellular carcinoma:Current evidence and future perspectives

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World Journal of Gastroenterology 2022年第27期28卷 3410-3421页

作者： masayuki Ueno Haruhiko Takeda Atsushi Takai Hiroshi Seno Department of Gastroenterology and Hepatology Kyoto University Graduate School of MedicineKyoto 6068507Japan

High rates of excessive calorie intake diets and sedentary lifestyles have led to a global increase in nonalcoholic fatty liver disease(NAFLD).As a result,this condition has recently become one of the leading causes of hepatocellular carcinoma(HCC).Furthermore,the incidence of NAFLD-associated HCC(NAFLD-HCC)is expected to increase in the near *** liver fibrosis is the most common risk factor for ***,up to 50%of NAFLDHCC cases develop without underlying liver *** studies have revealed many other risk factors for this condition;including diabetes,other metabolic traits,obesity,old age,male sex,Hispanic ethnicity,mild alcohol intake,and elevated liver *** gene variants,such as single-nucleotide polymorphisms of patatin-like phospholipase domain 3,transmembrane 6 superfamily member 2,and membrane-bound O-acyl-transferase domaincontaining 7,are also associated with an increased risk of HCC in patients with *** clinical and genetic information should be interpreted together for accurate risk ***-fetoprotein(AFP)is the only biomarker currently recommended for HCC ***,it is not sufficiently sensitive in addressing this diagnostic *** GALAD score can be calculated based on sex,age,lectin-bound AFP,AFP,and des-carboxyprothrombin and is reported to show better diagnostic performance for *** addition,emerging studies on genetic and epigenetic biomarkers have also yielded promising diagnostic ***,further research is needed to establish an effective surveillance program for the early diagnosis of NAFLD-HCC.

关键词： Nonalcoholic fatty liver disease Hepatocellular carcinoma Risk factors Biomarkers Tumor markers Genetics

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Luminescence study of europium(III) tris(β-diketonato)/phosphonate complexes in chloroform

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Journal of Rare Earths 2008年第2期26卷 185-191页

作者： Stefan Lis Zbigniew Piskula Krzysztof Staninski Sayaka Tamaki masayuki Inoue Yuko Hasegawa Department of Rare Earths Faculty of Chemistry Adam Mickiewicz University Grunwaldzka 6 Department of Chemistry Faculty of Science Science University of Tokyo

The extraction of Eu（Ⅲ） with β-diketone, HA, and monodentate or bidentate Lewis bases, B, into chloroform and the luminescence properties of the extracted species were studied. Pivaloyltrifluoroacetone, Hpta, and 2-thenoyltfifluoroacetone, Htta, were used as the β-diketones. The Lewis bases, B, were tetraethyl methylene diphosphonate, POPO, which was bidentate, and diethyl benzylphosphonate, PhPO which was monodentate. Based on the extraction data, the stability constants, log β, of the first complexes between tfis（β-diketonato）Eu（Ⅲ） and the phosphonate, EuA3B, were determined to be 6.0 for the POPO complex and 3.40 for the PhPO complex. The Eu（Ⅲ） luminescence intensity in the EuA3POPO was larger than EuA3 where A was either pta or tta at similar concentrations of Eu（Ⅲ）, while that in Eu（pta）3PhPO was stronger than EuA3; however, in Eu（tta）3PhPO, it was weaker than Eu（tta）3. The POPO functions as a sensitizer, and the PhPO functions as a quencher for the tta chelate and as a sensitizer for the pta chelate. From the lifetime and quantum yield, φ, of the Eu（Ⅲ） luminescence in the complexes as well as the observation of the extractability of Eu（Ⅲ） with the Hpta and the phosphonates and of the luminescence spectra of the complexes, it was confirmed that the extraction of Eu（Ⅲ） was remarkably enhanced with a β-diketonate and a strong Lewis base, and also the ternary complex that was formed as the extracted species, showed luminescence enhancement. This phenomenon may be due the formation of a strong bond between the Eu（Ⅲ） and the strong Lewis base leading to more hydrophobicity in the extracted species and also to more effective energy transfer from the Lewis base to the Eu（Ⅲ）. It was not significant whether the donor atoms were N or O.

关键词： tris(β-diketonato)Eu(Ⅲ) extraction luminescence phosphonate ligands rare earths

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INTRODUCTION TO GRIN LENS SYSTEMS

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光电子.激光 1989年第2期 70-73页

作者： ICHIRO kitano Central Research Laboratry Nippon sheet Glass Co.Ltd.

Gradient-index（GRIN） plano-convex lens optimizing its curvature with the refractive index distribution coefficients can attain the high numerical aperture aplanat. Highly specified lens system of either infinite and... 详细信息

关键词： mode INTRODUCTION TO GRIN LENS SYSTEMS length

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Recording the direct surgeon’s view with an operating microscopic view improves microscopic ophthalmic surgery training

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International Journal of Ophthalmology(English edition) 2023年第9期16卷 1555-1558页

作者： masayuki Akimoto Kosei Tomita Miyo Yoshida Yuki Hama Ophthalmology Osaka Red Cross HospitalTennoji-kuOsaka 543-8555Japan

AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to facilitate the reproduction of a skilled surgeon’s technique by a surgeon in ***:A small camera was attached to the operating microscope with a custom *** surgeon’s view and direct surgeon’s view through this new camera were recorded in the surgical recording *** movies were synchronized and analyzed how do surgeons handle the ***:A small camera attached to the operating microscope allowed the surgeon’s hands motion to be recorded without interfering with the surgeon’s *** surgeons used different methods to manipulate the ultrasound handpiece and the irrigation/aspiration *** in the simple paracentesis procedure,different surgeons used different ***-in-training were able to identify and improve their weaknesses by watching synchronized movies of their hand motions and microscopic ***:Simultaneous recording the surgical field out of the operating microscopic view by a small camera set on the microscope is comprehensive and improves surgeons-in-training understanding and learning surgeries.

关键词： operating microscopic view direct surgeon’s view video recording microscopic surgery training

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Influence of Si,Ce,Sb and Sn on chunky graphite formation

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China Foundry 2011年第1期8卷 96-100页

作者： Hideo Nakae masayuki Fukami Takayuki Kitazawa Ying Zou Lab. for Mater. Sci. and Tech. Waseda Univ. Tokyo Japan JFE Steel Corp. Japan Nippon Steel Corp. Japan Appl. Mech. and Eng. Waseda Univ. Tokyo Japan

The thirteen mother alloys,C%+1/3Si%=4.45%,differing in their Si,Ce,Sb and Sn contents,were *** grams of these alloys was remelted in a high purity alumina crucible at 1,450oC under an Ar atmosphere,and then cooled at 30 K/min for obtaining their cooling *** graphite morphologies were observed using an optical microscope and an *** three-dimensional graphite shapes were observed by the SEM using the samples whose matrices were etched off with an acid-aqua solution,to confirm the chunky *** discussing the influence of the Si and Ce contents on the chunky graphite formation,two experiments were carried *** the first one,the Si contents were changed from 0 to 4% in the 0.15%Ce alloys,and for the second one,the 3.5%Si and 4%Si samples that differed in the Ce contents of 0.1 and 0.2% were *** the third experiment,the influence of Sb and Sn on the chunky graphite formation was investigated by using the 4%Si and 0.1%Ce *** results showed that with the increase of the Si content,the volume fraction of the chunky graphite increases,while the volume fraction of the ledeburite decreases,and the chunky graphite volume fraction in the 0.2%Ce samples is higher than that of the 0.1%Ce *** effect of the Sb and Sn additions on the prevention of chunky graphite formation cannot be confirmed due to their high Si ***,further studies will be needed in this field.

关键词： chunky graphite spheroidal graphite graphite morphology solidification of cast iron

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