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Expression of the tumor suppressor gene maspin and its significance in intraductal papillary mucinous neoplasms of the pancreas

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 86-90页

作者： Kyoko Kashima Nobuyuki Ohike Seishiro Mukai masashi sato Manabu Takahashi Toshio Morohoshi Department of Pathology Showa University School of Medicine Department of Internal MedicineShowa University Toyosy Hospital Department of Pathology Showa University School of Medicine Department of Pathology Showa University School of Medicine Department of SurgeryShowa University School of Medicine Division of Pathology Ebara HospitalTokyo Japan

BACKGROUND: Maspin is a member of the serpin family of protease inhibitors and is thought to inhibit carcinoma invasion, metastasis, and angiogenesis and induce apoptosis. We examined maspin expression immunohistochemically and assessed its significance in intraductal papillary mucinous neoplasm (IPMN) of the pancreas. METHODS: We examined 39 surgically resected specimens of IPMN that included 17 adenomas (IPMAs), 5 borderline tumors (IPMBs), 4 non-invasive carcinomas (non-invasive IPMCs), and 13 invasive carcinomas (invasive IPMCs). Immunostaining was performed according to the EnVision ChemMate method. The degree of maspin expression was scored and assessed according to the percentage and staining intensity of positive cells. RESULTS: Maspin expression was minimal in normal pancreatic duct epithelium, whereas in IPMNs, maspin was expressed in neoplasms of all stages. Maspin expression increased with increasing grade from IPMAs, IPMBs, to non-invasive IPMCs but decreased significantly in invasive IPMCs. No specific association between maspin expression and mucin type was found. Analysis of maspin expression with respect to clinicopathologic factors in cases of invasive IPMC revealed a greater extent of invasion in cases of low maspin expression and significantly fewer apoptotic cells in the ***: Maspin was expressed at high levels in IPMNs at various stages from adenoma to invasive carcinoma, and our results suggest that maspin may be involved in the occurrence and progression of IPMN. In addition, our data suggest that the apoptosis-inducing action of maspin suppresses invasion and progression of IPMN.

关键词： maspin intraductal papillary mucinous neoplasm pancreas apoptosis immunohistochemistry

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Identification and Characterization of Human Genomic Binding Sites for Retinoic Acid Receptor/Retinoid X Receptor Heterodimers

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Advances in Biological Chemistry 2015年第2期5卷 58-72页

作者： Kenta Hosoda masashi sato Kazuyuki Yanai Department of Biomolecular Science Faculty of Science Toho University Chiba Japan

All-trans retinoic acid (ATRA) triggers a wide range of effects on vertebrate development by regulating cell proliferation, differentiation, and apoptosis. ATRA activates retinoic acid receptors (RARs) which heterodimerize with retinoid X receptors (RXRs). RAR/RXR heterodimers function as ATRA-dependent transcriptional regulators by binding to retinoic acid response elements (RAREs). To identify RAR/RXR heterodimer-binding sites in the human genome, we performed a modified yeast one-hybrid assays and identified 193 RAR/RXR heterodimer-binding fragments in the human genome. The putative target genes included genes involved in development process and cell differentiation. Gel mobility shift assays indicated that 160 putative RAREs could directly interact with the RAR/RXR heterodimer. Moreover, 19 functional regulatory single nucleotide polymorphisms (rSNPs) on the RAR/RXR-binding sequences were identified by analyzing the difference in the DNA-binding affinities. These results provide insights into the molecular mechanisms underlying the physiological and pathological actions of RAR/RXR heterodimers.

关键词： All-Trans Retinoic Acid Retinoic Acid Receptor Retinoid X Receptor Yeast One-Hybrid System Polymorphism Regulatory SNP

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Identification of CAR/RXR<i>α</i>Hetero-dimer Binding Sites in the Human Genome by a Modified Yeast One-Hybrid Assay

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Advances in Biological Chemistry 2015年第2期5卷 83-97页

作者： Kenta Hosoda Yuichiro Kanno masashi sato Jun Inajima Yoshio Inouye Kazuyuki Yanai Department of Biomolecular Science Faculty of Science Toho University Chiba Japan Faculty of Pharmaceutical Sciences Toho University Chiba Japan

The constitutive androstane receptor (CAR) is a transcription factor that belongs to the nuclear receptor superfamily. CAR binds as a heterodimer with the retinoid X receptor α (RXRα) to CAR response elements (CAREs) and regulates the expression of various drug metabolizing enzymes and transporters. To identify CAR/RXRα binding sites in the human genome, we performed a modified yeast one-hybrid assay that enables rapid and efficient identification of genomic targets for DNA-binding proteins. DNA fragments were recovered from positive yeast colonies by PCR and sequenced. A motif enrichment analysis revealed that the most frequent motif was a direct repeat (DR) of RGKTCA-like core sequence spaced by 4 bp. Next, we predicted 149 putative CAR/RXRα binding sites from 414 unique clones, by searching for DRs, everted repeats (ERs) and inverted repeats (IRs) of the RGKTCA-like core motif. Based on gel mobility shift assays, the CAR/RXRα heterodimer could directly interact with the 108 predicted sequences, which included not only classical CAREs but also a wide variety of arrangements. Furthermore, we identified 17 regulatory polymorphisms on the CAR/RXRα-binding sites that may influence individual variation in the expression of CAR-regulated genes. These results provide insights into the molecular mechanisms underlying the physiological and pathological actions of CAR/RXRα het-erodimers.

关键词： Constitutive Androstane Receptor Retinoid X Receptor Transcription SNP Polymorphism Nuclear Receptor

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Combination of simvastatin and hydroxyapatite fiber induces bone augmentation

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Open Journal of Regenerative Medicine 2013年第3期2卷 53-60页

作者： Shang Gao Makoto Shiota Masaki Fujii Kang Chen Masahiro Shimogishi masashi sato Shohei Kasugai Department of Oral and Maxillofacial Surgery Tokyo Medical and Dental University Tokyo Japan Department of Oral Implantology and Regenerative Dental Medicine Tokyo Medical and Dental University Tokyo Japan Department of Oral Implantology and Regenerative Dental Medicine Tokyo Medical and Dental University Tokyo Japan

This study evaluated the capability of hydroxyapatite fiber (HAF) as a carrier and the bone formation by blending simvastatin. The mixture of HAF and simvastatin (0.15, 0.45, 0.75 mg) was placed in 1 ml of tris-buffer and the release of simvastatin from HAF was calculated per 24 hours for 10 days. Bilateral 5 mm-diameter and 3 mm-hight Teflon chambers were fixed on calvaria of adult Japanese white rabbits and filled with 40 mg HAF which containing simvastatin (0, 0.15, 0.45, 0.75 mg). The animals were sacrificed at 4 and 8 weeks and calculated radiologically by Micro-CT. After dyeing by toluidine blue the samples were analyzed histologically. In all of the study groups approximately 25% of simvastatin was released until 10 days. The new bone volume ratio measured by Micro-CT of 4 and 8 weeks group was (22.4%, 21.3%, 41.6%, 26.3%) and (20.2%, 11.7%, 42.1%, 31.2%) in different doses respectively. The 0.45 mg group showed significantly higher new bone volume ratio than 0 mg group and 0.15 mg group. The histological measurement and observations also supported these results. In conclusion, the HAF could be used as a carrier for simvastatin. Combinations of HAF and simvastatin have the potentiality to stimulate new bone formation and approximately 0.45 mg simvastatin in 40 mg HAF is the optimal dose in rabbit chamber model.

关键词： Biomaterial Bone Substitutes Bone Formation Drug Delivery Growth Factors

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Attractants,Masking Substance and Oviposition Stimulants for the Cerambycid Beetle,Monochamus alternatus Hope,in Pine

Attractants,Masking Substance and Oviposition Stimulants for...

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The First Asia—Pacific Conference on Chemical Ecology

作者： TORU YAMASAKI masashi sato MITSURU SAKAI SYED Q.ISLAM Department of Biochemistry and Food Science Kagawa UniversityMiki-choKagawa 761-0795Japan

A newly emerged adult of the cerambycid beetle,Monochamus alternmatus Hope,holding dauer larvae, of the Parasitaphelenchid nematode,Bursaphelenchus xylophilus(Steiner et Buhrer) Nickle,is attracted by apinene (1).This suggests that attractant 1 leads the young adult to a healthy pine in *** larvae enter the pine tissues through the feeding wound formed on the twig by the *** a high mortality rate of pines has been caused by the combination of the beetle(vector) and the pathogenic pine wood nematode.

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Analysis of lichen substances including triterpenoids by high performance liquid chromatography with a differential refractive index detector and a photodiode array detector

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菌物学报 2011年第6期30卷 944-949页

作者： Hikari sato Kojiro HARA masashi KOMINE Yoshikazu YAMAMOTO Graduate School of Bioresource Sciences Akita Prefectural University:Shimoshinjo-nakanoAkita 010-0195Japan

A new method for analysis of lichen triterpenoids was established using high performance liquid chromatography with the combination of a differential refractive index detector(RID) and a photodiode array detector(PDA).It is proved that this method was convenient to detect and identify aromatic and aliphatic lichen substances;it enabled quantitative analysis of substances having no or less absorption of ultraviolet rays such as *** addition,they can be measured in high accuracy compared with the TLC method.

关键词：三萜系化合物青苔 RID 菌物学

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Association of non-synonymous variants in WIPF3 and LIPA genes with ab- dominal aortic aneurysm： an autopsy study

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Journal of Geriatric Cardiology 2016年第12期13卷 960-967页

作者： Yuko Maeda Noriko sato Makiko Naka-Mieno Seijiro Mori Tomio Arai masashi Tanaka Masaaki Muramatsu Motoji Sawabe Department of Molecular Epidemiology Medical Research Institute Tokyo Medical and Dental University Tokyo Japan Department of Medical Informatics Center of Inormation Jichi Medical University Tochigi Japan Center for Promotion of Clinical Investigation Tokyo Metropolitan Geriatric Hospital Tokyo Japan Department of Pathology Tokyo Metropolitan Geriatric Hospital Tokyo Japan Research Teams for Geriatric Pathology Tokyo Metropolitan Institute of Gerontology Tokyo Japan Department of Genomics for Longevity and Health Tokyo Metropolitan Institute of Gerontology Tokyo Japan Department of Molecular Pathology Graduate School of Health Care Sciences Tokyo Medical and Dental University Tokyo Japan

腹的大动脉的动脉瘤(AAA ) 是有强壮的基因部件的 multifactorial 疾病。各种各样的基因 loci 与临床的 AAA 被联系了，但是很少研究调查了病理学的 AAA， *** 的中间的显型在日本个人(JG-SNP 学习) 在衰老老人疾病上从研究检验了更老... 详细信息

腹的大动脉的动脉瘤(AAA ) 是有强壮的基因部件的 multifactorial 疾病。各种各样的基因 loci 与临床的 AAA 被联系了，但是很少研究调查了病理学的 AAA， *** 的中间的显型在日本个人(JG-SNP 学习) 在衰老老人疾病上从研究检验了更老的日本题目的 2263 具连续尸体。AAA 的存在在尸体期间与病理学的诊断被决定。与 AAA 联系的单个核苷酸变体(SNV ) 与一个 Illumina HumanExome Beadchip 数组被决定。逻辑回归分析被执行决定基因协会。当有 AAA 的 ***118 题目和没有 AAA 的 2145 个题目在一个盒子控制背景被分析，年龄，性，和 AAA 的另外的风险因素被分析。没有变体在使用 Bonferroni 修正以后到达了意义(P < 2.05 ???????  ???????????????????? 瀠瑡敩瑮 ? 潃据畬楳湯味敨 ?????????????? 吗？？

关键词：腹的大动脉的动脉瘤 Lysosomal 酸脂肪分解酵素 A WAS/WASL 交往蛋白质家庭 3

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A possible substellar companion to the intermediate-mass giant HD 175679

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Research in Astronomy and Astrophysics 2012年第1期12卷 84-92页

作者： Liang Wang Bun＇ei sato Gang Zhao Yu-Juan Liu Kunio Noguchi Hirovasu Ando Hideyuki Izumiura Eiji Kambe masashi Omiya Hiroki Harakawa Fan Liu Xiao-Shu Wu Yoichi Takeda Michitoshi Yoshida Eiichiro Kokubo Key Laboratory of Optical Astronomy National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Department of Earth and Planetary Sciences Graduate Sclaooi of Science and EngineeringTokyo Institute of Technology 2-12-1 Ookayama Meguro-ku Tokyo 152-8551 Japan National Astronomical Observatory of Japan National Institutes of Natural Sciences. MitakaTokyo 181-8588 Japan Okayama Astrophysical Observatory National Astronomical Observatory of Japan NationalInstitutes of Natural Sciences Asakuchi Okayama 719-0232 Japan Hiroshima Astrophysical Science Center Hiroshima University -1-3-1 KagamiyamaHigashi-Hiroshima 739-8526 Japan

We report the discovery of a substellar companion around the intermediate- mass giant HD 175679. Precise radial velocity data of the star from the Xinglong Station and the Okayama Astrophysical Observatory revealed a Keplerian velocity variation with an orbital period of 1366.8±5.7 d, a semiamplitude of 380.2±3.2 m s-1 and an eccentricity of 0.378± 0.008. Adopting a stellar mass of 2.7± 0.3 M, we obtain that the minimum mass of the HD 175679 b is 37.3± 2.8 M and the semimajor axis is 3.36±0.12 AU. This discovery is the second brown dwarf companion candidate from a joint planet-search program between China and Japan.

关键词： stars: individual (HD 175679) -- stars: brown dwarfs -- techniques:radial velocities

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Radiofrequency ablation in a porcine liver model:Effects of transcatheter arterial embolization with iodized oil on ablation time,maximum output,and coagulation diameter as well as angiographic characteristics

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World Journal of Gastroenterology 2007年第20期13卷 2841-2845页

作者： Motoki Nakai Morio sato Shinya Sahara Nobuyuki Kawai Hirohiko Tanihata masashi Kimura Masaki Terada Department of Radiology Hidaka General Hospi-tal116-2 SonoGobo ShiWakayama 644-8655Japan Department of Radiol-ogy Wakayama Medical University 811-1KimiideraWakayama ShiWakayama 641-8510Japan

AIM:To evaluate the effects of combined radiofrequen-cy ablation and transcatheter arterial embolization with iodized oil on ablation time,maximum output,coagula-tion diameter,and portal angiography in a porcine liver model. METHODS: Radiofrequency ablation (RFA) was applied to in vivo livers of 10 normal pigs using a 17-gauge 3.0 cm expandable LeVeen RF needle electrode with or with-out transcatheter arterial embolization (TAE) with iodized oil (n = 5). In each animal,2 areas in the liver were ab-lated. Direct portography was performed before and af-ter RFA. Ablation was initiated at an output of 30 W,and continued with an increase of 10 W per minute until roll-off occurred. Ablation time and maximum output until roll-off,and coagulated tissue diameter were compared between the 2 groups. Angiographic changes on portog-raphy before and after ablation were also reviewed. RESULTS: For groups with and without TAE with iodized oil,the ablation times until roll-off were 320.6 ± 30.9 seconds and 445.1 ± 35.9 seconds,respectively,maxi-mum outputs were 69.0 ± 7.38 W and 87.0 ± 4.83 W and maximal diameters of coagulation were 41.7 ± 3.85 mm and 33.2 ± 2.28 mm. Significant reductions of abla-tion time and maximum output,and significantly larger coagulation diameter were obtained with RFA following TAE with iodized oil compared to RFA alone. Portography after RFA following TAE with iodized oil revealed more occlusion of the larger portal branches than with RFA alone. CONCLUSION: RFA following TAE with iodized oil can increase the volume of coagulation necrosis with lower output and shorter ablation time than RFA alone in nor-mal pig liver tissue.

关键词：射频消融猪动物实验肠疾病动脉栓塞

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Smoking increases risk of tooth loss: A meta-analysis of the literature

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World Journal of Meta-Analysis 2013年第1期1卷 16-26页

作者： Fumihito sato masashi Sawamura Miki Ojima Keiko Tanaka Takashi Hanioka Hideo Tanaka Keitaro Matsuo Division of Epidemiology and Prevention Aichi Cancer Center Research Institute Nagoya Aichi 464-8618 Japan Department of Preventive Dentistry Graduate School of Dentistry Osaka University Suita Osaka 565-0871 Japan Department of Public Health Faculty of Medicine Fukuoka University Fukuoka 814-0180 Japan Department of Preventive and Public Health Dentistry Fukuoka Dental College Fukuoka 814-0193 Japan Department of Epidemiology Nagoya University Graduate School of Medicine Nagoya Aichi 464-8601 Japan

AIM: To quantitatively evaluate the impact of smoking on tooth ***: We performed a Pub Med search to identify published articles that investigated the risk of tooth loss by smoking, from which RRs and their variance with characteristics of each study were extracted. The random-effects models were used to derive a pooled effect across studies. Potential sources of heterogeneity on the characteristics of the study and their influence on the pooled effect size were investigated using metaregression models. RESULTS: We identified 24 studies containing a total of 95973 participants for analysis. The pooled RR of ever-smokers compared with never- smokers was 1.73(95%CI: 1.60-1.86, P < 0.001). In meta-regression analysis, only the mean age of participants alone was identified as a statistically significant source of heterogeneity. The effect of smoking on tooth loss was stronger when the mean age of study participants was higher, indicating possible enhancement of tooth loss due to aging by smoking. RR was significantly lower in former smokers(1.49, 95%CI: 1.32-1.69, P < 0.001) than in current smokers(2.10, 95%CI: 1.87-2.35, P < 0.001), indicating the substantial benefit of smoking cessation for reducing the risk of tooth ***: Smoking is an independent risk factor for tooth loss regardless of many other confounders. Smoking cessation may attenuate this effect.

关键词： Meta-analysis Oral health Relative risk Smoking Tooth loss

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