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Determination of Telomere Length by the Quantitative Fluorescence <i>in Situ</i>Hybridization (Q-FISH) Method

American Journal of Analytical Chemistry 2014年第12期5卷 775-783页

作者： Junko Aida Naotaka Izumiyama-Shimomura Ken-ichi Nakamura Naoshi Ishikawa masanori terai Yoko Matsuda Shinsuke Aida Tomio Arai Kaiyo Takubo Department of Pathology International University of Health and Welfare Mita Hospital Tokyo Japan Department of Pathology Tokyo Metropolitan Geriatric Hospital Tokyo Japan Research Team for Geriatric Pathology Tokyo Metropolitan Institute of Gerontology Tokyo Japan

Telomeres are nucleoprotein complexes located at the ends of eukaryotic chromosomes and are shorten with aging or various causes. Shortened telomere plays an important role for chromosomal instability in carcinogenesis, or a number of diseases in relation to aging. FISH using peptide-nucleic acid (PNA) probe is suitable for analyzing telomeres, because telomere is a part of DNA molecule and localized in chromosomal end in loop shape structure (T-loop). We introduce our telomere analysis for tissue sections and cultured cells in this paper. On the tissue sections, we analyze telomere length with telomere intensity to centromere intensity ratio (TCR), centromere as an inner control. In the cultured cells, we analyze telomere lengths on each chromosome with karyotyping. Those Q-FISH methods by PNA probe are an accurate and reliable tool for research on telomere lengths in various conditions in biology.

关键词： Telomere Telomere Length Q-FISH Tissue Section Chromosome

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Usefulness of autotaxin for the complications of liver cirrhosis

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World Journal of Gastroenterology 2020年第1期26卷 97-108页

作者： Xue Shao Haruki Uojima Toru Setsu Tomomi Okubo masanori Atsukawa Yoshihiro Furuichi Yoshitaka Arase Hisashi Hidaka Yoshiaki Tanaka Takahide Nakazawa Makoto Kako Tatehiro Kagawa Katsuhiko Iwakiri Shuji terai Wasaburo Koizumi Department of Gastroenterology Internal MedicineKitasato University School of MedicineSagamiharaKanagawa 252-0375Japan Department of Hepatopancreatobiliary Medic Department of Gastroenterology Shonan Kamakura General HospitalKamakuraKanagawa 247-8533Japan Division of Gastroenterology and Hepatology Graduate School of Medical and Dental SciencesNiigata UniversityNiigata 951-8510Japan Department of Internal Medicine Division of GastroenterologyNippon Medical School Chiba Hokusoh HospitalChiba 270-1694Japan Department of Internal Medicine Division of Gastroenterology and HepatologyNippon Medical SchoolTokyo 113-8603Japan Department of Gastroenterology and Hepatology Tokyo Medical University HospitalShinjukuTokyo 113-8510Japan Division of Gastroenterology and Hepatology Department of Internal MedicineTokai University School of MedicineKanagawa 259-1193Japan

BACKGROUND Autotaxin(ATX)has been reported as a direct biomarker for estimating the evaluation of liver *** available data on ATX as a useful biomarker for the complications of liver cirrhosis(LC)are *** To assess the clinical usefulness of ATX for assessing the complications of *** This multicenter,retrospective study was conducted at six locations in *** include patients with LC,n=*** ATX level was evaluated separately in men and women because of its high level in female *** assess the clinical usefulness of ATX for the complications of LC,the area under the curve(AUC)of ATX assessing for the severe complications was analyzed in comparison with the model for end-stage liver disease score,albumin-bilirubin(ALBI)score,fibrosis-4 index,and aspartate aminotransferase-to-platelet ratio *** The mean age was 68.4±11.4 years,240 patients(60.0%)were male.A total of 213(53.3%)and 187(46.8%)patients were compensated and decompensated,*** numbers of patients with varix rupture,hepatic ascites,and hepatic encephalopathy were 35(8.8%),131(32.8%),and 103(25.8%),*** AUCs of ATX in men for hepatic encephalopathy,hepatic ascites,and varix ruptures were 0.853,0.816,and 0.706,*** AUCs of ATX in women for hepatic encephalopathy,hepatic ascites,and varix rupture were 0.759,0.717,and 0.697,*** AUCs of ATX in men were higher than those in women,as were all the other biomarkers used to detect encephalopathy and varix ***,for detecting ascites,the AUC of ALBI in men was more effective than using *** ATX in men was more effective than any other biomarkers for detecting hepatic encephalopathy and varix ruptures.

关键词： Autotaxin Hepatic encephalopathy Hepatic ascites Liver fibrosis Biomarker

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