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mediator contributes to IgH locus VDJ rearrangements by promoting usage of most distal V segments

Cellular & molecular Immunology 2020年第4期17卷 407-409页

作者： Iman Dalloul Zeinab Dalloul sandrine Le Noir François Boyer Bernardo Reina-san.m.rtin Jeanne Cook-moreau michel Cogné UmR CNRS 7276 INSERM U1262Universite de LimogesLimoges 87025France INSeRm U1258 UMR CNRS 7104Institut de Genetique et de Biologie Moleculaire et CellulaireUniversitéde StrasbourgIllkirch 67400France

INTRODUCTION The immunoglobulin heavy(IgH)chain locus includes a large cluster of V,D and J segments spanning thousands of kilobases in mammals and its expression implies long-range 3D-regulation of various gene modifications.1 V(D)J recombination occurs during early B-cell ontogeny.2 It is regulated by multiple transcription factors and cis-regulatory elements,and most notably by the intronic 5′eμelement located in the JH to Cμintron.3 The intergenic control region 1(IGCR1)located between the VH and D gene clusters inhibits rearrangement of the most DH-proximal VH gene segments,while promoting usage of distal VH gene segments.4 Other downstream elements of the locus,flanking its 3′end might have more subtle roles:while the large 3′regulatory region(3′RR)shows strong internal synergies2 and behaves as a superenhancer in mature B *** deletion showed no influence on the VDJ repertoire and rather increased transcription of unrearranged V segments.3–5 Insulators binding CTCF farther downstream of the 3′RR were shown,however,to promote usage of distal VH segments.6 Altogether,there are strong indications that elements involved in long-distance regulation of transcription might also influence VDJ ***-frame splicing of VDJ exons is also needed for full Ig expression.7 Given the functional importance of gene architecture and long-range interactions for the IgH locus physiology,we explored the dependence of the VDJ repertoire on med1,a known actor of long-range enhancer interactions.

关键词： B cells mediator VDJ repertoire

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Acute myocardial infarction in myeloproliferative neoplasms

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World Journal of Cardiology 2023年第11期15卷 571-581页

作者： muhammad Romail manan Vincent Kipkorir Iqra Nawaz maryann Wanjiku Waithaka Bahadar Singh Srichawla Amelia maria Găman Camelia Cristina Diaconu mihnea-Alexandru Găman Services Institute of medical Sciences Lahore 54000Pakistan Department of Human Anatomy and Physiology University of NairobiNairobi 00100Kenya Quaid-e-Azam medical College Bahawalpur 63100Pakistan Department of Neurology University of Massachusetts Chan Medical SchoolWorcesterMA 01655United States Department of Pathophysiology University of Medicine and Pharmacy of CraiovaCraiova 200143Romania Clinic of Hematology Filantropia City HospitalCraiova 200143Romania Department of Internal medicine Faculty of Medicine“Carol Davila”University of Medicine and PharmacyBucharest 050474Romania Internal medicine Clinic Clinical Emergency Hospital of BucharestBucharest 105402Romania Faculty of medicine “Carol Davila”University of Medicine and PharmacyBucharest 050474Romania Department of Hematology Center of Hematology and Bone Marrow TransplantationFundeni Clinical InstituteBucharest 022328Romania Department of Cellular and molecular Pathology Stefan S.Nicolau Institute of VirologyRomanian AcademyBucharest 030304Romania

myeloproliferative neoplasms(mPNs)are a heterogeneous group of hematologic malignancies characterized by an abnormal proliferation of cells of the myeloid *** individuals are at increased risk for cardiovascular and thrombotic *** infarction(mI)may be one of the earliest clinical manifestations of mPNs or may be a thrombotic complication that develops during the natural course of the *** the present review,we examine the epidemiology,pathogenesis,clinical presentation,and management of mI in mPNs based on the available ***,we review potential biomarkers that could mediate the mI-mPNs crosstalk,from classical biochemical tests,e.g.,lactate dehydrogenase,creatine kinase and troponins,to pro-inflammatory cytokines,oxidative stress markers,and clonal hematopoiesis.

关键词： myeloproliferative neoplasms Polycythemia vera essential thrombocythemia myelofibrosis myocardial infarction Acute coronary syndrome Biomarker Clonal hematopoiesis

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Influence of flooding and vegetation on carbon,nitrogen,and phosphorus dynamics in the pore water of a Spartina alterniflora salt marsh

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Journal of environmental Sciences 2011年第2期23卷 212-221页

作者： Vanesa L.Negrin Carla V.Spetter Raúl O.Asteasuain Gerardo m.e.Perillo Jorge e.marcovecchio Argentine Institute of Oceanography (IADO CONICETCCT-Bahía Blanca)Camino La Carrindanga km 75Building E-1 CC 804Bahía Blanca 8000Argentina Department of Chemistry Southern National Institute of Chemistry (INQUISUR)Southern National University (UNS)Av Alem 1253Bahía Blanca 8000Argentina Department of Geology Southern National University (UNS)San Juan 670Bahía Blanca 8000Argentina National Technological University Regional Faculty of Bahía Blanca (UTN-FRBB)11 de Abril 461Bahía Blanca 8000Argentina University of the Fraternity of Associations santo Toms de Aquino (FASTA) Gascón 3145Mar del Plata 7600Argentina

Four sites were selected in a salt marsh in the Bahia Blanca estuary （Argentina）：（1） low marsh （flooded by the tide twice daily） vegetated by S. alterniflora; （2） non-vegetated low marsh; （3） high marsh （flooded only in spring tides） vegetated by S. alterniflora; （4） non-vegetated high marsh. The pH and eh were measured in sediments, while dissolved nutrients （ammonium, nitrate, nitrite and phosphate） and particulate organic matter （POm） were determined in pore water, pH （6.2-8.7） was only affected by vegetation in low areas. eh （from -300 to 250 mV） was lower at low sites than at high ones; in the latter, the values were higher in the non-vegetated sediments. The POm concentration was greater in the high marsh than in the low marsh, with no effect of vegetation. Ammonium was the most abundant nitrogen nutrient species in pore water, except in the non-vegetated high marsh where nitrate concentration was higher. All nitrogen nutrients were affected by both flooding and vegetation. Phosphate was always present in pore water at all sites throughout the year and its concentration varied within narrow limits, with no effect of flooding and greater values always at non-vegetated sites. Our results showed that the variability of the pore water composition within the marsh is greater than the temporal variation, meaning that both tidal flooding and vegetation are important in the dynamics of nutrients and organic matter in the sediment pore water.

关键词： organic matter nutrients flooding Spartina alterniflora

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钛合金叶轮石墨型离心铸造工艺优化

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特种铸造及有色合金 2014年第4期34卷 413-415页

作者：伞晶超辽宁装备制造职业技术学院

介绍了某化工设备上使用的钛合金叶轮铸件石墨型铸造工艺。分析了铸件产生气孔和叶片位置偏移等缺陷的原因。通过增加加工余量、提高离心转速和优化石墨型工艺,即加工余量由原来的5mm增至15mm、离心转速由120r/min提高至200r/min并制作... 详细信息

介绍了某化工设备上使用的钛合金叶轮铸件石墨型铸造工艺。分析了铸件产生气孔和叶片位置偏移等缺陷的原因。通过增加加工余量、提高离心转速和优化石墨型工艺,即加工余量由原来的5mm增至15mm、离心转速由120r/min提高至200r/min并制作划线工装,消除了铸件缺陷,最终获得了合格的钛合金叶轮。

关键词：钛合金叶轮石墨型离心铸造

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Photocatalytic performance of K2Ti6O13 whiskers to H2 evolution and CO2 photo-reduction

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Journal of energy Chemistry 2019年第10期28卷 18-28页

作者： L. F. Garay-Rodríguez L.m.Torres-martínez e.moctezuma Facultad de Ingeniería Civil-Departamento de ecomateriales y energía Universidad Autónoma de Nuevo LeónAv. Universidad S/N Cd. UniversitariaC. P. 66455 San Nicolás de los GarzaNLMéxico Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíAv. Manuel Nava#6C. P.78290 San Luis PotosíSLPMéxico

K2Ti6O13 whiskers were synthesized by conventional sol-gel method, sono-chemical assisted and microwave assisted sol-gel method in order to obtain catalysts with different particle sizes and to modify their optical, textural and electrochemical properties. These modifications improved their photocatalytic activity for H2 evolution and CO2 photo-reduction. Long K2Ti6O13 whiskers prepared by ultrasound assisted sol-gel method are the most active photocatalysts for the hydrogen evolution reaction using pure water as reactant (U-SG, 10,065 μmol g^-1). In contrast, an opposite behavior was observed using a mixture of ethanol-water, where the highest activity was achieved by the shortest and less crystalline K2Ti6O13 whiskers (C-SG, 3,2871 μmol g^-1). In case of CO2 photo-reduction, long whiskers that were also prepared by the sono-chemical assisted sol-gel method were the most active to transform CO2 to formaldehyde, methane, methanol and hydrogen. The eFB value of this catalyst is located very close to the potential for formaldehyde production and favors the selectivity to this organic product.

关键词： K2Ti6O13 Sol-gel Whiskers Hydrogen evolution CO2 reduction

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乙型脑炎病毒囊膜蛋白I176R位点突变株的生物学特性比较研究

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农业生物技术学报 2024年第10期32卷 2391-2401页

作者：唐冕耀赵勤石艳萍曹玉琴文翼平伍锐杜森焱黄小波颜其贵曹三杰四川农业大学动物医学院/猪病研究中心四川农业大学国家级动物类实验教学示范中心农业农村部兽用药物与兽医诊断技术四川科学观测站

乙型脑炎病毒(Japanese encephalitis virus, JeV)是一种危害严重的人畜共患病毒，囊膜蛋白(envelope protein, e)对JeV的神经毒力起关键作用。前期研究发现，重组乙型脑炎病毒(recombinant Japanese encephalitis virus, rJeV)囊膜蛋白I176R位点突变可致弱其神经毒力。为鉴定rJeV-eI176R位点突变后其生物学特性的变化，本研究克隆分析了e176位点突变重组病毒rJeV-eI176R的e基因序列和e蛋白的生物信息学特征；利用qPCR、蚀斑试验和间接免疫荧光(indirect immunofluorescence assay, IFA)等技术测定rJeV-eI176R的生长曲线、对多种细胞的吸附差异和rJeV-eI176R诱导小鼠小胶质细胞系(mouse microglia cell line, BV-2)细胞炎性水平差异。结果显示，rJeV-eI176R的e蛋白带正电荷的残基增加1个，等电点升高0.37，不稳定指数升高0.39;e蛋白的α螺旋、β折叠、延伸链以及无规则卷曲的数量及占比均发生了改变，同时e176位点氨基酸与附近氨基酸有了不同的连接；rJeV-eI176R在BV-2细胞上的生长曲线没有显著变化且能够感染不同来源的神经细胞和肾细胞；rJeV-eI176R的吸附能力显著提高(PeV-eI176R接种BV-2细胞后，在12和24 h均引起肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-6 (interleukin-6, IL-6)、干扰素γ诱导蛋白-10 (interferongamma-induced protein-10, IP-10)的水平显著低于亲本株(PeV毒株e蛋白的生物学特性研究，为后期深入研究JeV减毒机制和疫苗研发提供基础资料。

关键词：乙型脑炎病毒(JeV) 囊膜蛋白(e) I176R位点突变神经毒力致弱生物学特性

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Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation

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Cell Research 2001年第1期11卷 44-54页

作者： ZAmORANO JOSe,Unidad de Investigacion, Hospital san Pedro de Alcantara, Avda millan Astray, 10003 Caceres ANN e KeLLY, JONATHAN AUSTRIAN, HeLeN Y WANG, ACHSAH D KeeGAN (Department of Immunology, Jerome Holland Labs, American Red Cross, Rockville, mD, USA Department of Immunology Jerome Holland Labs American Red Cross Rockville USA Unidad de Investigacion Hospital San Pedro de Alcantala Avda Millan Astray Caceres

IL-4 is an important B cell survival and growth factor. IL-4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS- or CD40L-activated blasts. Phosphorylated IRS2 coprecipitated with the p85 subunit of PI 3’ kinase in both resting and activated cells. By contrast, association of phosphorylated IRS2 with GRB2 was not detected in resting B cells after IL-4 treatment although both proteins were expressed. However, IL-4 induced association of IRS2 with GRB2 in B cell blasts. The pattern of IL-4- induced recruitment of p85 and GRB2 to IRS2 observed in B cells derived from STAT6 null mice was identical to that observed for normal mice. While IL-4 alone does not induce activation of meK, a meKI inhibitor suppressed the IL-4-induced proliferative response of LPS-activated B cell blasts. These results demonstrate that costimulation of splenic B cells alters IL-4-induced signal transduction independent of STAT6 leading to proliferation. Furthermore, proliferation induced by IL-4 in LPS-activated blasts is dependent upon the mAP kinase pathway.

关键词： B lymphocytes IL-4 survival proliferation.

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实数遗传算法的改进及性能研究

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电子学报 2007年第2期35卷 269-274页

作者：任子武伞冶哈尔滨工业大学控制与仿真中心黑龙江哈尔滨150001

提出一种粒子群优化方法(PSO)与实数编码遗传算法(GA)相结合的混合改进遗传算法(HIGAPSO).该方法采用混沌序列产生初始种群、非线性排序选择、多个交叉后代竞争择优和变异尺度自适应变化等改进遗传操作;并通过精英个体保留、粒子群优化... 详细信息

提出一种粒子群优化方法(PSO)与实数编码遗传算法(GA)相结合的混合改进遗传算法(HIGAPSO).该方法采用混沌序列产生初始种群、非线性排序选择、多个交叉后代竞争择优和变异尺度自适应变化等改进遗传操作;并通过精英个体保留、粒子群优化及改进遗传算法(IGA)三种策略共同作用产生种群新个体,来克服常规算法中收敛速度慢、早熟及局部收敛等缺陷.通过四个高维典型函数测试结果表明该方法不但显著提高了算法的全局搜索能力,加快了收敛速度;而且也改善了求解的质量及其优化结果的可靠性,是求解优化问题的一种有潜力的算法.

关键词：遗传算法粒子群优化方法竞争择优变异尺度

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THe NeXT meCHANICAL ReVOLUTION AND THe LAWS OF HUmAN NATURe

机械技术史

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机械技术史 1998年 16-40页

作者： C.C.Wang,3e Software,BOX 612345,san Jose,CA 95161-2345

The quantum theory developed at the beginning of this century has established the foundation for a law of nature and led to the discovery of DNA and RNA—the codes of *** spectacular successful applications in both medical and agricultural fields,another new frontier is being opened by the researchers at the NASA/Ames research center and elsewhere across the United States to investigate the possibility of imitating the way nature builds organisms for new ways of building machines. Tough questions are therefore raised:can a revolution be raised inside the mechanical domain without the involvement of mechanical engineers? What do today’s mechanical engineers do when the manipulation of atoms/molecules as material building blocks guided by DNA-like replication control becomes successful? Should mechanical engineers at that time warn the next mechanical revolution pioneers the same way as our most trusted computational tool developers have warned us in the use of mSC-NASTRAN:"In the rush to automate,you must keep in mind that no technology available today can take the place of engineering judgement".There was no instruction with regards to how to nurture or to measure one’s engineering *** money incentive hidden in the loopholes of today’s doctrines of democracy,free enterprise, and academic freedom,have formidable power that is far greater than can be *** hidden incentive offers an attractive bonus to those who cheat or goof,but imposes the severest penalty to those who are honest and highly efficient,In competing for profit,the human nature is good at learning shortcuts and being ***,unless there is a balance of power in the form of noble judgement created from within,or very strict disciplines and effective scrutiny imposed to counter-balance acting from without, the outrageous moral trickling down is bound to *** the perfect disciplines and effective scrutiny are very difficulty to establish,the easiest thing

关键词： Next mechanical revolution Laws of human nature

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酒精性肝纤维化核因子κB信号通路与性别的差异

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解剖学报 2024年第1期55卷 55-61页

作者：洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉河南科技大学基础医学与法医学院肝脏损伤与修复分子医学重点实验室河南科技大学第一附属医院麻醉科河南省肝病防治工程技术研究中心河南洛阳471000

目的探讨酒精性肝纤维化的核因子(NF)-κB信号通路与性别差异的关系。方法将7~8周龄的C57BL/6 N小鼠随机分为:雄性正常组、雄性模型组,雌性正常组和雌性模型组各20只。正常组采用对照液体饲料喂养8周,模型组采用酒精液体饲料喂养8周,联... 详细信息

目的探讨酒精性肝纤维化的核因子(NF)-κB信号通路与性别差异的关系。方法将7~8周龄的C57BL/6 N小鼠随机分为:雄性正常组、雄性模型组,雌性正常组和雌性模型组各20只。正常组采用对照液体饲料喂养8周,模型组采用酒精液体饲料喂养8周,联合31.5%乙醇灌胃(每周两次,5 g/kg)建立酒精性肝纤维化模型。8周末处死小鼠,检测各组小鼠血清学谷丙转氨酶(ALT)及谷草转氨酶(AST)的活性,雌二醇(e2)和睾酮(T)的水平,天狼星红染色检测各组小鼠纤维化情况,He染色观察肝组织病理学变化,免疫组织化学法检测NF-κB-P65和α平滑肌肌动蛋白(α-SmA)阳性面积率,免疫印迹法检测Ⅰ型胶原蛋白(collagenⅠ)、肿瘤坏死因子α(TNF-α)、磷酸化核因子κB-P65(p-NF-κB-P65)、核因子κB抑制因子α(IκBα)、磷酸化核因子κB抑制因子α(p-IκBα)的表达水平。结果雄性模型组ALT,AST酶活性和T的水平升高,e2的水平降低,胶原纤维增加,He染色坏死积分升高,NF-κB-P65和α-SmA的阳性面积率增加,collagenⅠ,TNF-α、p-NF-κB-P65、p-IκBα的表达增加,IκBα的表达降低。结论酒精更容易导致雄性小鼠肝纤维化,这可能与加强IκBα磷酸化释放NF-κB,促使磷酸化NF-κB-p65增多,进一步激活NF-κB信号通路有关。

关键词：酒精性肝纤维化 NF-κB信号通路性别差异免疫印迹法小鼠

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