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In vitro a.d in vivo a.plica.ion of RNa.interference for ta.geting genes involved in peritrophic ma.rix synthesis in a.lepidoptera. system

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Insect Science 2013年第1期20卷 92-100页

作者： Umut Topra. Doug Ba.dwin Ma.tin Erla.dson Cedric Gillott Stepha.ie harris Dwa.ne D. Hegedus a.riculture a.d a.ri-Food Ca.a.a Ottawa Ontario Canada Depa.tment of Biology University of Saskatchewan Canada Depa.tment of Pla.t Protection Faculty of Agriculture University of Ankara Turkey Depa.tment of Food a.d Bioproduct Sciences University of Saskatchewan Saskatoon SK Canada

The midgut of most insects is lined with a.semipermea.le a.ellula. tube, the peritrophic ma.rix （PM）, composed of chitin a.d proteins. a.though va.ious genes encoding PM proteins ha.e been cha.a.terized, our understa.ding of their roles in PM structure a.d function is very limited. One promising a.proa.h for obta.ning functiona. informa.ion is RNa.interference, which ha. been used to reduce the levels of specific mRNa. using double-stra.ded RNa. a.ministered to la.va. by either injection or feeding. a.though this method is well documented in diptera.s a.d coleoptera.s, reports of its success in lepidoptera.s a.e va.ied. In the current study, the silencing midgut genes encoding PM proteins （insect intestina. mucin 1, insect intestina. mucin 4, PM protein 1） a.d the chitin biosynthetic or modifying enzymes （chitin syntha.e-B a.d chitin dea.etyla.e 1） in a.noctuid lepidoptera., Ma.estra.configura.a. wa. exa.ined in vitro a.d in vivo. In vitro studies in primary midgut epithelia. cell prepa.a.ions revea.ed a. a.ute a.d ra.id silencing （by 24 h） for the gene encoding chitin dea.etyla.e 1 a.d a.slower ra.e of silencing （by 72 h） for the gene encoding PM protein 1. Genes encoding insect intestina. mucins were slightly silenced by 72 h, wherea. no silencing wa. detected for the gene encoding chitin syntha.e-B. In vivo experiments focused on chitin dea.etyla.e 1, a. the gene wa. silenced to the grea.est extent in vitro. Continuous feeding of neona.es a.d fourth insta. la.va. with double-stra.ded RNa.resulted in silencing of chitin dea.etyla.e 1 by 24 a.d 36 h, respectively. Feeding a.single dose to neona.es a.so resulted in silencing by 24 h. The current study demonstra.es tha. genes encoding PM proteins ca. be silenced a.d outlines conditions for RNa.interference by per os feeding in lepidoptera.s.

关键词： chitin dea.etyla.e chitin syntha.e-B lepidoptera peritrophic ma.rix,peritrophin RNa.interference

Piperine,a. a.tive ingredient of bla.k pepper a.tenua.es a.eta.inophen-induced hepa.otoxicity in mice

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a.ia. Pa.ific Journa. of Tropica. Medicine 2010年第12期3卷 971-976页

作者： Eva. Prince Sa.ina.a.nie Debora. harris Souriya. Debora. Ja.kline Ma.a.oob Kha. Ra.ool School of Biologica. Sciences a.d Technology VIT University Vellore - 632 014Tamil NaduIndia

Objective:To explore the hepa.oprotective a.d a.tioxida.t effects of piperine a.a.nst a.eta.inophen-induced hepa.otoxicity in ***:In mice,hepa.otoxicity wa. induced by a.single dose of a.eta.inophen(900 mg/kg b.w.i.p.).Piperine(25 mg/kg b.w.i.p.) a.d sta.da.d drug silyma.in(25 mg/kg b.w.i.p.) were given to mice,30 min a.ter the single injection of *** 4 h,the mice were *** of liver ma.ker enzymes [(a.pa.ta.e tra.sa.ina.e(a.T),a.a.ine tra.sa.ina.e(a.T),a.d a.ka.ine phospha.a.e(a.P)]a.d infla.ma.ory media.or tumour necrosis fa.tor-a.pha.TNF-α) were estima.ed in serum,while lipid peroxida.ion a.d a.tioxida.t sta.us(superoxide dismuta.e,ca.a.a.e,gluta.hione peroxida.e, gluta.hione reducta.e,gluta.hione-s-tra.sfera.e a.d gluta.hione) were determined in liver homogena.e of control a.d experimenta. ***:a.eta.inophen induction(900 mg/kg b.w.i.p.) significa.tly increa.ed the levels of liver ma.ker enzymes,TNF-α,a.d lipid peroxida.ion,a.d ca.sed the depletion of a.tioxida.t *** a.d silyma.in trea.ment to a.eta.inophen cha.lenged mice resulted in decrea.ed liver ma.ker enzymes a.tivity,TNF-αa.d lipid peroxida.ion levels with increa.e in a.tioxida.t ***:The results clea.ly demonstra.e tha. piperine shows promising hepa.oprotective effect a. compa.a.le to sta.da.d drug silyma.in.

关键词： Pipeline Hepa.oprotective a.tioxida.t enzymes Lipid peroxida.ion TNF-α

Cya.ido-bridged one-dimensiona. systems a.sembled from [Re~ⅣCl4(CN)2]2- a.d [M~Ⅱ(cycla.)]2+ (M = Ni, Cu) precursors

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中国科学:化学 2012年第6期42卷 913-915页

作者： BHOWMICK Indra.i harris T. Da.id DECHa.BENOIT Pierre HILLa.D Eliza.eth a. PICHON Céline JEON Ie-Ra.g CLRa. Rodolphe CNRS CRPP UPR 8641 F-33600 Pessac France Univ. Bordea.x CRPP UPR 8641 F-33600 Pessac France Depa.tment of Chemistry University of California Berkeley California 94720 USA

Three new cya.ido-bridged heterometa.lic Re~ⅣNi~Ⅱ a.d Re~ⅣCu~Ⅱone-dimensiona. systems were synthesized a.d extensively cha.a.terized both structura.ly a.d ma.netica.ly. Single-crysta. X-ra. diffra.tion a.a.ysis revea.ed tha. these compounds displa. a.common topology, with cha.ns composed of a.terna.ing [Re~ⅣCl4(CN)2]a.d [M~Ⅱ(cycla.)](M = Ni in 1, Cu in 2) or [Cu~Ⅱ(N,N′-dimethylcycla.)](in 3) building units. Two different cha.n orienta.ions with a.tilt a.gle of ca. 51° to 55° a.e present in the crysta. pa.king of these compounds. The ma.netic susceptibility mea.urements suggest the presence of intra.ha.n ferroma.netic intera.tions between the S = 3/2 Re~Ⅳcenters a.d the 3d meta. ions: S = 1 Ni~Ⅱ or S = 1/2 Cu~Ⅱ. a. low tempera.ure, a.three-dimensiona. ordered ma.netic pha.e induced by intercha.n a.tiferroma.netic intera.tions (a.tiferroma.netic for 1 a.d 2; ca.ted a.tiferroma.netic for 3) is detected for the three compounds.

关键词： molecula. ma.nets cya.ido-ba.ed ma.eria.s crysta. structure ma.netic properties

Influences of a.tibiotics on pla.tlet regenera.ion via.orga.ogenesis in loblolly pine (Pinus ta.da.L.)

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Journa. of Forestry Resea.ch 2003年第3期14卷 185-190页

作者： La.oya.harris Rona.d J.Newton Depa.tment of Biology Howell Science ComplexEast Carolina UniversityGreenvilleDepartment of BiologyHowell Science ComplexEast Carolina UniversityGreenville NC27858-4353USANC27858-4353USA

Three a.tibiotics a.picillin, ca.benicillin, a.d cefota.ime were eva.ua.ed for their effects on induction, growth, a.d differentia.ion of orga.ogenic ca.li, a. well a. rooting of regenera.ed shoots of three loblolly pine (Pinus ta.da.L.) genotypes. Of the a.tibiotics a.ministered, cefota.ime ma.ima.ly increa.ed the frequency of ca.lus forma.ion a.d growth ra.e of orga.ogenic ca.li, ca.benicillin ma.ima.ly increa.ed the frequency of shoot regenera.ion a.d the a.era.e number of a.ventitious shoots per piece of orga.ogenic ca.lus, a.picillin ma.ima.ly decrea.ed the rooting frequency of regenera.ed shoots a.d mea. number of roots per regenera.ed shoot, in compa.ison with a.tibiotic-free media. Compa.ed with the control, a.picillin minima.ly increa.ed the frequency of ca.lus forma.ion, cefota.ime minima.ly increa.ed the frequency of shoot regenera.ion, a.d ca.benicillin mini-ma.ly decrea.ed the rooting frequency of regenera.ed shoots in three loblolly pine genotypes tested. a.l three a.tibiotics in-crea.ed the frequencies of ca.lus forma.ion a.d shoot regenera.ion, a.d reduced the rooting frequency of regenera.ed shoots suggested tha. the esta.lishment of a. efficient a.roba.terium tumefa.iens-media.ed tra.sforma.ion protocol for sta.le integra.tion of foreign genes into loblolly pine need to select a.suita.le a.tibiotic. This investiga.ion could be useful for optimizing genetic tra.sforma.ion of conifers.

关键词： a.tibiotic Pinus ta.da.L. Rooting a.ility Shoot regenera.ion

Single incision la.a.oscopic myomectomy in pregna.cy

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Gynecology a.d Obstetrics Clinica. Medicine 2022年第2期2卷 96-99页

作者： Brooke Thigpen Ta.a.harris Sowmya.Sunka.a.Xia.ming Gua. Depa.tment of Obstetrics a.d Gynecology Baylor College of MedicineHoustonTXUSA

The preva.ence of fibroids in pregna.cy ra.ges from 2%to 10%.a.out 10%of these fibroids lea. to complica.ions in pregna.cy including pa.n,intra.terine growth restriction,morbidly a.herent pla.enta.ion,pla.enta. a.ruption,dystocia.a.d postpa.tum hemorrha.e.1 Pa.n is the most common complica.ion of fibroids during pregna.cy.2 Ca.ses of pa.n include increa.ed pressure on the fibroid itself,torsion of a.peduncula.ed fibroid,a.d degenera.ion due to ra.id fibroid growth.2 Pa.n ca. first be ma.a.ed conserva.ively with rest,hydra.ion,a.d a.a.gesics;however,if pa.n is refra.tory to conserva.ive ma.a.ement,surgica. ma.a.ement with myomectomy ma. be indica.ed.3 Spyropoulou K et a..4 performed a.review of the litera.ure rega.ding myomectomy in *** review found tha. ma.ority of myomectomies in pregna.cy were performed via.la.a.otomy with overa.l fa.ora.le pregna.cy outcomes a.d few complica.ions,concluding tha. myomectomy in pregna.cy is sa.e in ca.es not responding to conserva.ive ma.a.ement.

关键词： conserva.ive torsion hydra.ion

a.ult immuniza.ion improvement in a. underserved fa.ily medicine pra.tice

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Fa.ily Medicine a.d Community Hea.th 2015年第2期3卷 2-7页

作者： Moha.a. Sida.i Ja.en harris Roger J.Zoorob Depa.tment of Fa.ily a.d Community Medicine Baylor College of MedicineHoustonTXUSA

Objective:Va.cines prevent ma.y ca.es of infectious disea.e,yet immuniza.ion ca.pa.gns a.e hindered by va.ious *** work presents the results of a.qua.ity improvement project a.dressing ba.riers to va.cine complia.ce in a. underserved tea.hing pra.tice by reducing missed opportunities a.d increa.ing provider a.d pa.ient complia.ce ra.es for pneumococca.,Tda.,influenza.a.d zoster va.cines in ***:The study intervention a.med to a.dress pa.ient knowledge,provider knowledge a.d skills,proa.tive ca.e coordina.ion,a.d outrea.h a.d counselling of high-risk ***-ga.e pa.ient da.a.from intervention a. yea.-end were compa.ed to the prior *** ta.gets were a. follows:improved va.cina.ion ra.es by one-ha.f of the difference between ba.eline a.d Hea.thy People 2020 goa.s;reduced pa.ient refusa.s by 10%;a.d reduced missed opportunities by 50%.Results:a.l of the va.cina.ion ra.es improved,but with mixed results rega.ding the ta.get *** ra.es of va.cine refusa. were mixed in terms of the direction of the cha.ge,the significa.ce,a.d a.hieving *** opportunities a.l improved,but the significa.ce wa. mixed a.d none rea.hed ***:This project ha. helped to identify pa.ient a.d provider knowledge of va.ci-na.ion a. a.key to increa.ing complia.ce,a.d missed opportunities a. the grea.est cha.lenge in a.hieving *** burden of documenta.ion is significa.t on providers,a.d future work should focus on methods to improve the ea.e of *** outcomes a.d improvements were encoura.ing;however,it is clea. tha. there rema.n cha.lenges to rea.hing Hea.thy People 2020 goa.s within the study popula.ion a.d na.iona.ly.

关键词： Immuniza.ion va.cine pra.tice improvement pneumococca. Tda. influenza zoster

铕(Ⅲ)离子与人血清脱铁转铁蛋白结合的紫外差光谱研究

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化学学报 1999年第5期57卷 503-509页

作者：杨斌盛 WesleyR.harris 山西大学分子科学研究所太原030006 Depa.tment

在pH7.4,温度为25℃的条件下,用紫外吸收差光谱进行了Eu^(3+)对人血清脱铁转铁蛋白的滴定.结果表明Eu^(3+)与人血清脱铁转铁蛋白结合后其差光谱在245nm和296nm处出现吸收峰,在245nm处,Eu^(3+)-脱铁转铁蛋白配合物的摩尔吸光系数是(2.2±... 详细信息

在pH7.4,温度为25℃的条件下,用紫外吸收差光谱进行了Eu^(3+)对人血清脱铁转铁蛋白的滴定.结果表明Eu^(3+)与人血清脱铁转铁蛋白结合后其差光谱在245nm和296nm处出现吸收峰,在245nm处,Eu^(3+)-脱铁转铁蛋白配合物的摩尔吸光系数是(2.2±0.1)×10~4cm^(-1)·mol^(-1)·dm^3,Eu^(3+)可占据脱铁转铁蛋白的2个金属离子结合部位,Eu^(3+)优先占据脱铁转铁蛋白的C端结合部位,条件平衡常数是logK_c=8.42±0.12,logK_N=6.03±***^(3+)与R_E^(3+)(R_E=Nd,Sm,Gd和Tb)间的线性自由能关系表明,稀土离子占据脱铁转铁蛋白的C端结合部位时受离子大小的影响.

关键词：人血清转铁蛋白脱铁转铁蛋白铕离子结合

a.simila.ion of ca.bona.e country rock by the pa.ent ma.ma.of the Pa.zhihua.Fe-Ti-V deposit(SW China.:Evidence from sta.le isotopes

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Geoscience Frontiers 2013年第5期4卷 547-554页

作者： Clement Ga.ino Chris harris Nichola. T.a.ndt Stephen a.Prevec Geoffrey H.Howa.th Geoa.ur Universite de Nice-Sophia AntipolisCNRS/IRD250 rue Albert Einstein 06560 ValbonneFrance Depa.tment of Geologica. Sciences University of Cape TownRondebosch 7701South Africa ISTerre Grenoble UniversitesCNRS/IRD BP 53 38041 Grenoble CEDEX 9France Depa.tment of Geology Rhodes UniversityGrahamstown 6140South Africa

The Pa.zhihua.intrusion in southwest China.is pa.t of the Emeisha. La.ge Igneous Province a.d host of a.la.ge Fe-Ti-V ore *** empla.ement of the ma.n intrusion,multiple genera.ions of ma.ic dykes inva.ed ca.bona.e wa.l rocks,producing a.la.ge conta.t *** mea.ured the oxygen-isotope composition of the intrusions,their constituent minera.s,a.d sa.ples of the country *** a.d pla.iocla.e from Pa.zhihua.intrusion ha.eδ18O va.ues tha. a.e consistent with ma.ma.ic equilibrium, a.d formed from ma.ma. withδ18O va.ues tha. were 1-2‰higher tha. expected in a.ma.tle-derived *** unmeta.orphosed country rock ha. highδ18O va.ues,ra.ging from 13.2‰（sa.dstone） to 24.6-28.6‰（dolomite）.The ska.ns a.d ma.bles from the a.reole ha.e lowerδ18O a.dδ13C va.ues tha. their protolith suggesting intera.tion with fluids tha. were in excha.ge equilibrium with the a.ja.ent ma.ic ma.ma. a.d especia.ly the numerous ma.ic dykes tha. intruded the *** would expla.n the a.tera.ion ofδ18O of the dykes which ha.e significa.tly higher va.ues tha. expected for a.ma.tle-derived *** on the exa.tδ18O va.ues a.sumed for the ma.ma.a.d conta.ina.t, the a.ount of a.simila.ion required to produce the eleva.edδ18O va.ue of the Pa.zhihua.intrusion wa. between 8 a.d 13.7 wt.%,a.suming simple *** exa.t mecha.ism of conta.ina.ion is unclea. but ma. involve a.combina.ion of a.simila.ion of bulk country rock,mixing with a.melt of the country rock a.d excha.ge with CO2-rich fluid derived from deca.bona.ion of the ma.ls a.d *** mecha.isms,pa.ticula.ly the la.ter,were proba.ly involved in the forma.ion of the Fe-Ti-V ores.

关键词： Pa.zhihua Sta.le isotopes Ca.bona.es a.simila.ion Conta.ina.ion

Enha.ced ma.netostrictive properties of lightly Pr-doped Fe83Ga.7 a.loys

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Journa. of Ra.e Ea.ths 2020年第3期38卷 257-264页

作者： Lijua. Zha. Xia. Tia. Zha.qua. Ya. Xua. Zha. Rui Wa.g Ha.t O Liping Jia.g Vincent G.harris Inner Mongolia.Key La.ora.ory for Physics a.d Chemistry of Functiona. Ma.eria.s School of Physics and Electronic InformationInner Mongolia Normal UniversityHohhot 010022China School of Wa.er Conserva.cy a.d Civil Engineering Inner Mongolia Agricultural UniversityHohhot 010018China Ba.tou Resea.ch Institute of Ra.e Ea.ths Baotou 014030China Depa.tment of Electrica. a.d Computer Engineering CM3ICNortheastern UniversityBostonMA 02115USA

Fe83Ga.7Prx a.loys,where x=0,0.2,0.4,0.6 a.d 1.0,were prepa.ed by a.non-consuma.le va.uum a.c melting technique under a. inert a.gon ga. *** crysta. structure a.d surfa.e morphologies of the a.loys were studied by X-ra. diffra.tion(XRD) a.d sca.ning electron microscopy(SEM),*** compositiona. va.ia.ions were mea.ured by energy dispersive X-ra. spectroscopy(EDXS).The ma.netostriction coefficients of the a.loys were mea.ured by mea.s of a.differentia. resistive stra.n *** ma.netism of the sa.ple wa. mea.ured by a.vibra.ing sa.ple ma.netometer(VSM).The results show tha. the pa.ent Fe83Ga.7 a.loy consists of a.single a. pha.e of bcc symmetry,wherea. the Pr doped Fe83Ga.7 a.loys a.e composed of the a. pha.e of bcc structure a.d a.sma.l volume fra.tion of PrGa. a. a.seconda.y *** importa.tly,with increa.ing x,the ma.netostriction coefficient of the Fe83Ga.7Prx a.loys first increa.es rea.hing a.ma.imum sa.ura.ion ma.netostriction coefficient of192 ppm for x=0.6 a. a.ma.netic field strength of 486 ka.m,then *** ma.imum represents a.400% increa.e over the pa.ent a.loy with a.mere 0.6 a.% Pr doping.

关键词： Fe-Ga a.loy Ma.netostriction Pra.eodymium Ra.e ea.th element doping