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Impact of Genetic Diversity of Uropathogenic Escherichia coli and Klebsiella pneumoniae Strains on the Dissemination of Extended Spectrum Beta-Lactam Resistance Genes in Côte d’Ivoire

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American Journal of Molecular Biology 2024年第4期14卷 230-244页

作者： Innocent Allepo Abe martial kassi n’djetchi Mélika Barkissa Traore Flora Yao Thomas Konan Konan Paulin Didier Sokouri Ibrahim Konate Mathurin Koffi Laboratoire de Biodiversit et Gestion durable des Ecosystmes Tropicaux Unit de Recherche en Gntique et Epidmiologie Molculaire (URGEM) UFR Environnement Universit Jean Lorougnon Gud Daloa Cte dIvoire Laboratoire dAgrovalorisation UFR Agroforesterie Universite Jean Lorougnon Guede Daloa Cote dIvoire Laboratoire de Biotechnologie Agriculture et Valorisation des ressources biologiques UFR Biosciences Universit Flix Houphout-Boigny Abidjan Cte dIvoire Laboratoire dAmelioration de la production agricole Unite de Recherche en Genetique et Epidemiologie Moleculaire (URGEM) UFR Agroforesterie Universite Jean Lorougnon Guede Daloa Cte dIvoire

The increase and spread of bacterial resistance to extended-spectrum beta-lactam antibiotics are reported in many infections and are a real public health problem worldwide. Drug pressure is a factor that favors the emergence of a population of better adapted bacteria. However, there is no literature highlighting the genetic diversity and evolutionary structure of E. coli and K. pneumoniae in an environment with high selection pressure in Côte d’Ivoire. The objective of this study was to evaluate the genetic diversity of E. coli and K. pneumoniae strains circulating at the HKB Hospital in Abobo and at the Daloa Regional Hospital and its impact on the dissemination of extended spectrum beta-lactam resistance genes. A total of 39 strains isolated from the urinary tract of infected patients, including 30 strains of E. coli and 9 strains of K. pneumoniae were studied. A total of 39 strains isolated from the urinary tract of infected patients, including 30 strains of E. coli and 9 strains of K. pneumoniae were studied. From genomic DnA extracts, ESBL resistance genes were amplified by PCR and sequenced, in addition to genetic typing by ERIC-PCR. The data obtained were submitted to genetic and bioinformatics analyses. The results have shown a genetic diversity important in E. coli and K. pneumoniae with diversity indexs (SID) ranging from 0.5 to 0.77. The genetic structure of the bacterial species studied has shown a clonal distribution of strains with clones expressing TEM-9 and CTX-M-15 variants. Also, this clonal structure was correlated with the spread of resistance genes in E. coli and K. pneumoniae. The spread of resistant clones is a factor that might limit the fight against antibiotic resistance.

关键词： Escherichia coli Klebsiella pneumoniae Extended-Spectrum β-Lactam Antibiotic Resistance Genetic Diversity

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Specificity of serological screening tests and reference laboratory tests to diagnose gambiense human African trypanosomiasis: a prospective clinical performance study

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Infectious Diseases of Poverty 2024年第4期13卷 48-63页

作者： martial kassi n'djetchil Oumou Camara Mathurin Koffi Mamadou Camara Dramane Kaba Jacques Kabore Alkali Tall Brice Rotureau Lucy Glover Melika Barkissa Traorel Minayegninrin Kone Bamoro Coulibaly Guy Pacome Adingra Aissata Soumah Mohamed Gassama Abdoulaye Dansy Camara Charlie Franck Alfred Compaore Aissata Camara Salimatou Boiro Elena Perez Anton Paul Bessell nick Van Reet Bruno Bucheton Vincent Jamonneau Jean-Mathieu Bart Philippe Solano Sylvain Bieler Veerle Lejon Laboratory of Biodiversity and Ecosystem Management Jean Lorougnon Guede UniversityDaloaCote d'ivoire national Program for neglected Tropical Disease Control Patient ManagementMinistry of HealthConakryGuinea Trypanosomosis Research Unit Pierre Richet InstituteBouakeCote d'ivoire International Research and Development Centre on Livestock in Subhumid Zones Bobo-DioulassoBurkina Faso national Program for Malaria Control ConakryGuinea Parasitology Unit Institut Pasteur de GuineeConakryGuinea Trypanosome Molecular Biology Unit Department of Parasites and Insect VectorsPasteur InstituteParis Cite UniversityParisFrance Intertryp IRD-CIRAD-University of MontpellierMontpellierFrance Independent Consultant EdinburghUk Department of Biomedical Sciences Institute of Tropical MedicineAntwerpBelgium Foundation for Innovative new Diagnostics GenevaSwitzerland

Background Serological screening tests play a crucial role to diagnose gambiense human African trypanosomiasis(gHAT).Presently,they preselect individuals for microscopic confrmation,but in future"screen and treat"strategies they willidentify individuals for *** in reported specificities,the development of new rapid diagnos-tic tests(RDT)and the hypothesis that malaria infection may decrease RDT specificity led us to evaluate the specificity of 5 gHAT screening *** During active screening,venous blood samples from 1095 individuals from Cote d'ivoire and Guinea were tested consecutively with commercial(CATT,HAT Sero-K-SeT,Abbott Bioline HAT 2.0)and prototype(DCn HAT RDT,HAT Sero-K-SeT 2.0)gHAT screening tests and with a malaria *** with≥1 positive gHAT screening test underwent microscopy and further immunological(trypanolysis with *** LiTat 1.3,1.5 and 1.6;indirect ELISA/***;*** inhibition ELISA with *** LiTat 1.3 and 1.5 VSG)and molecular reference laboratory tests(PCR TBRn3,18S and TgsGP;SHERLOCK 18S Tids,7SL Zoon,and TgsGP;Trypanozoon S2-RT-qPCR 18S2,177T,GPl-PLC and TgsGP in multiplex;RT-qPCR DT8,DT9 and TgsGP in multiplex).Microscopic trypanosome detection confrmed gHAT,while other individuals were considered gHAT *** in fractions between groups were assessed by Chi square and differences in specificity between 2 tests on the same individuals by *** One gHAT case was *** test specificities(n=1094)were:CATT 98.9%(95%CI:98.1-99.4%);HAT Sero-K-SeT 86.7%(95%CI:84.5-88.5%);Bioline HAT 2.082.1%(95%CI:79.7-84.2%);DCn HAT RDT 78.2%(95%CI:75.7-80.6%);and HAT Sero-K-SeT 2.078.4%(95%CI:75.9-80.8%).In malaria positives,gHAT screening tests appeared less specific,but the difference Was significant only in Guinea for Abbott Bioline HAT 2.0(P=0.03)and HAT Sero-K-Set 2.0(P=0.0006).The specificities of immunological and molecular laboratory tests in gHAT seroposit

关键词： Human African trypanosomiasis Trypanosoma brucei gambiense Diagnosis Specificity Rapid diagnostic test Immunoloaical test Molecular test

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Evaluation of the Effect of Four Levels of Shade on the Growth and Development of Desmodium adscendens with a View to Its Domestication as a Cover Crop in Côte d’Ivoire

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Agricultural Sciences 2024年第11期15卷 1179-1194页

作者： Konan Didier Kouame Kouadio Jacques-Edouard Yao Koffi Fernand Jean-martial kassi Kouadio Yann Henoc Kouadio Klinnanga noël Kone Daouda Kone UFR Biosciences UPR Plant Physiology University Flix Houphout-Boigny Abidjan Cte dIvoire UFR Agroforestry UPR Plant Physiology University Jean Lorognon Gud Daloa Cte dIvoire UFR of Biological Sciences University Peleforo Gon Coulibaly Korhogo Cte dIvoire

Desmodium adscendens is a plant in the Fabaceae family that is very important to man, both agronomically and medicinally. It is an important cover crop that contributes to improving soil fertility, reducing the risks associated with crop pests and weeds. However, very little research has been carried out into its domestication for use as a cover crop in banana plantations in Côte d’Ivoire. The general objective of this study was to evaluate, under semi-controlled conditions, the effect of different levels of shade on the growth and development of Desmodium adscendens. The studies were carried out on the experimental site of the UP-Plant Physiology Laboratory. Four shading levels were evaluated: 0 (nO), 20% (n1), 40% (n2) and 60% (n3) light levels. The Desmodium adscendens cuttings used were collected from a 3-month nursery. For each treatment, 10 tubs containing sterilized soil were used, and 50 cuttings were planted. After planting, growth and development parameters were assessed weekly on 10 plants. In addition, yield and fresh and dry mass were measured. Finally, the reproductive cycle of Desmodium adscendens was determined. The results showed that plant growth and development were greatest in plants grown under the 40% shade level, followed by the 20% and 60% shade levels. In contrast, plants in full light showed poor growth and development. The 40% shade level was the most favorable for growing Desmodium adscendens cuttings, with a cycle length of 67 days. Growing Desmodium adscendens under shade with 20% and 40% light levels could be recommended to farmers.

关键词： Desmodium adscendens Shade Levels Cover Crops Domestication—Côte d’Ivoire

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