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Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

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Open Journal of Animal Sciences 2020年第2期10卷 254-265页

作者： Sofia Lamas Filipa Franquinho marlene morgado Fátima Gartner Irina Amorim Institute for Research and Innovation in Health (i3S) University of Porto Porto Portugal Animal Facility i3S/IBMC Porto Portugal Institute for Molecular and Cell Biology (IBMC) Porto Portugal Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP) Porto Portugal Department of Pathology and Molecular Immunology Institute of Biomedical Sciences Abel Salazar (ICBAS) University of Porto Porto Portugal

Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo development, this investigation aimed to compare two alternatives for 2-cells embryo collection in order to reduce the number of females and to benefit from the superovulation process. Data from mouse embryo collection from our facility was analyzed to compare the number of 2-cells embryos collected at 1.5 dpc and the number of 2-cells embryos obtained after overnight incubation of 1-cell embryos, collected at 0.5 dpc. Genetically modified mouse strains with a similar background (C57BL/6J, B6*129 and FVB/NJ) were analyzed and for strains at a C57BL/6J and B6*129 background, the number of 2-cells embryos obtained after incubation was significantly higher when compared to the number of 2-cells embryos collected at 1.5 dpc (1.4-fold and 1.7-fold, respectively). C57BL/6J wild type mice had similar results with a higher number of 2-cells embryos when collection was performed at 0.5 dpc followed by incubation (1.4-fold). These results can help the planning of 2-cells embryo harvesting by reducing the number of females needed for this procedure.

关键词： 0.5 Dpc Embryos 1.5 Dpc Embryos Incubation Superovulation

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Electroactivity of the magnetotactic bacteria Magnetospirillum magneticum AMB-1 and Magnetospirillum gryphiswaldense MSR-1

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Frontiers of Environmental Science & Engineering 2024年第4期18卷 103-110页

作者： Mathias Fessler Qingxian Su marlene Mark Jensen Yifeng Zhang Department of Environmental and Resource Engineering Technical University of DenmarkCopenhagenDK-2800Denmark

Magnetotactic bacteria reside in sediments and stratified water *** are named after their ability to synthesize internal magnetic particles that allow them to align and swim along the Earth’s magnetic field ***,we show that two magnetotactic species,Magnetospirillum magneticum strain AMB-1 and Magnetospirillum gryphiswaldense strain MSR-1,are *** *** and *** were able to generate current in microbial fuel cells with maximum power densities of 27 and 11μW/m^(2),*** the presence of the electron shuttle resazurin both species were able to reduce the crystalline iron oxide hematite(Fe_(2)O_(3)).In addition,*** could reduce poorly crystalline iron oxide(FeOOH).Our study adds *** and *** to the growing list of known electroactive bacteria,and implies that electroactivity might be common for bacteria within the Magnetospirillum genus.

关键词： Magnetotactic bacteria Magnetospirillum magneticum Magnetospirillum gryphiswaldense Extracellular electron transfer Microbial fuel cells

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Hepatic grooves:An observational study at laparoscopic surgery

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World Journal of Experimental Medicine 2024年第2期14卷 97-102页

作者： Shamir O Cawich Dexter A Thomas Fawwaz Mohammed Michael T Gardner marlene Craigie Shaneeta Johnson Ramnanand S Kedambady Department of Surgery University of the West IndiesSt Augustine 000000Trinidad and Tobago Department of Surgery Port of Spain General HospitalPort of Spain 000000Trinidad and Tobago Section of Anatomy Basic Medical SciencesUniversity of the West IndiesKingston 000000Jamaica Department of Radiology University of the West IndiesKingston 000000Jamaica Department of Surgery Morehouse School of MedicineAtlantaGA 30310United States Section of Anatomy Department of Basic Medical SciencesUniversity of the West IndiesKingston KIN7Jamaica

BACKGROUND In traditional descriptions,the upper surface of the liver is smooth and convex,but deep depressions are variants that are present in 5%-40%of *** sought to determine the relationship between surface depressions and the *** To use exploratory laparoscopy to determine the relationship between surface depressions and the *** An observational study was performed in all patients undergoing laparoscopic upper gastro-intestinal operations between January 1,2023 and January 20,2024.A thirty-degree laparoscope was used to inspect the liver and *** surface depressions were present,we recorded patient demographics,presence of diaphragmatic bands,rib protrusions and/or any other source of compression during *** Of 394 patients,343 had normal surface anatomy,and 51(12.9%)had prominent surface depressions on the *** was no significant relationship between the presence of surface depressions and gender nor the presence of rib ***,there was significant association between the presence of surface depressions and diaphragmatic muscular bands(P<0.001).CONCLUSION With these data,the diaphragmatic-band theory has gained increased importance over other theories for surface *** studies are warranted using cross sectional imaging to confirm relationships with intersectional planes as well as beta-catenin assays in the affected liver parenchyma.

关键词： Liver Variant Vein Hepatic Surgery

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为生活做预算——规划人员退休计划

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中国会展 2024年第8期 32-39页

作者： marlene Goldman 不详

会议和活动可以提供信息和乐趣,但在某些时候,是时候退休了。提前制定计划至关重要。对于会议策划者来说,活动管理的关键之一是要从长远的角度考虑如何在预算范围内制定解决方案。规划者在为个人生活做预算和计划时,包括为自己的退休生... 详细信息

会议和活动可以提供信息和乐趣,但在某些时候,是时候退休了。提前制定计划至关重要。对于会议策划者来说,活动管理的关键之一是要从长远的角度考虑如何在预算范围内制定解决方案。规划者在为个人生活做预算和计划时,包括为自己的退休生活做计划时,也可以利用同样的深谋远虑。可以花多少钱以及如何在预算内生活设定一个框架,这既适用于成功的会议,也适用于个人生活。

关键词：预算范围退休生活深谋远虑规划者策划者退休计划解决方案

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食品和饮料趋势简单和健康满足可持续性

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中国会展 2024年第14期 36-43页

作者： marlene Goldman 不详

提供自助餐是给与会者提供选择的一个简单方法。去年在拉斯维加斯举行的SEMA展会上,为SEMA(专业设备市场协会)理事会和网络成员举办了活动。协会策划人员面临的一个关键挑战是在活动期间跟上与会者对食品和饮料的偏好。

关键词：策划人员网络成员自助餐可持续性拉斯维加斯 SEMA 饮料食品

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混合型会议活动正在形成一种文化

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中国会展 2024年第10期 36-41页

作者： marlene Goldman 不详

在新冠疫情引发的所有趋势中,混合型会议和活动可能会在未来几年持续下去。随着2023年即将结束,许多金融和保险会议策划者很高兴地看到,大多数与会者比以往任何时候都更喜欢亲自参加会议。但是,正如活动现场与会者的活力激发了整个行业... 详细信息

在新冠疫情引发的所有趋势中,混合型会议和活动可能会在未来几年持续下去。随着2023年即将结束,许多金融和保险会议策划者很高兴地看到,大多数与会者比以往任何时候都更喜欢亲自参加会议。但是,正如活动现场与会者的活力激发了整个行业的活力一样,混合选项的灵活性也激发了虚拟人群的活力。问题是,接下来会发生什么?

关键词：策划者活力激发灵活性文化混合型虚拟人群会议

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赞助终极的社交媒体

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中国会展(中国会议) 2024年第18期 32-35页

作者： marlene Goldman

所有类型的广告都不缺乏渠道，同样的规则也适用于赞助。从协会会议到运动队再到社区活动，赞助是一个无处不在的营销机会。一些策划者更进一步说，facebook是各种规模企业的终极社交媒体。赞助为人们提供了一个强大的平台，让人们面对...

所有类型的广告都不缺乏渠道，同样的规则也适用于赞助。从协会会议到运动队再到社区活动，赞助是一个无处不在的营销机会。一些策划者更进一步说，facebook是各种规模企业的终极社交媒体。赞助为人们提供了一个强大的平台，让人们面对面联系并成为非凡活动的一部分，为策划者提供了一个独特的收入机会，以抵销活动的成本。

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Prevalence and Factors Associated with Anemia among People Living with Human Immunodeficiency Virus Followed at the Outpatient Treatment Centre of Panzi General Referral Hospital in Eastern Democratic Republic of Congo

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Journal of Biosciences and Medicines 2024年第3期12卷 259-274页

作者： marlene Abedi Zalufa Jean-Paulin Mbo Mukonkole Aline Byabene Kusinza Nicolas Vignier Dieudonné Bihehe Masemo David Willy Shamputi Omari Mukanga Lampard Marie Hatem Department of Internal Medicine Panzi General Referral Hospital Bukavu Democratic Republic of Congo Evangelical University in Africa Bukavu Democratic Republic of Congo Statistics Department Panzi General Referral Hospital Bukavu Democratic Republic of Congo International Centre for The Advanced Research and Training-ICART Panzi Foundation Bukavu Democratic Republic of Congo Faculty of Medicine University of Kisangani Kisangani Democratic Republic of Congo Service des Maladies Infectieuses et Tropicales CHU Avicenne Bobigny France Sorbonne University Paris France School of Public Health Department of Social and Preventive Medicine Université de Montréal Montréal Canada

Introduction: Anemia in people living with human immunodeficiency virus (PLHIV) is a major health problem. Although anemia often responds to combination antiretroviral therapy, many patients remain anemic despite treatment, and such persistent anemia continues to adversely affect prognosis, regardless of drug response. Scientists have identified some of the factors involved. However, the mechanisms put in place have not been effective in overcoming them. Examples include the withdrawal of zidovudine from antiretroviral treatment lines, iron and folate supplementation, etc. Anemia is still a major concern in HIV-positive patients. The aim of this study is to assess the prevalence of anemia and its associated factors among PLHIV followed up at the outpatient treatment centre (CTA) of the Panzi General Reference Hospital (HGR) in South Kivu, Democratic Republic of Congo (DRC). Method: We conducted a cross-sectional, comparative study of 276 HIV-infected adults on antiretroviral therapy (ART) followed up at the CTA of Panzi HGR. Socio-demographic and nutritional parameters were collected using a survey questionnaire, and clinical assessment and nutritional status were performed at the centre. Hemoglobin, seric albumin and viral load determinations were performed at the HGRP laboratory. We constructed univariate and multivariate logistic regression models to assess factors associated with anemia in people living with HIV/AIDS. Results: We found a prevalence of anemia of 39.4%, including 4.1% severe anemia, 17.7% moderate anemia and 17.5% mild anemia. After multivariate adjustment, the factors associated with anemia in our PLHIV were: moderate undernutrition (aOR = 1.26;95% CI: 1.50 - 4.20;p = 0.001), severe undernutrition (aOR = 115.4;95% CI: 2.04 - 164.52;p = 0.021), hypoalbuminemia (aOR = 2.11;95% CI: 1.87 - 5.10;p = 0.004) and the lower degree of dietary diversity (aOR = 1.56;95% CI: 1.10 - 4.32;p = 0.034). Conclusion: The prevalence of anemia in PLHIV on ART is high.

关键词： Anemia HIV/AIDS Undernutrition PANZI Hospital

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Sperm limitation affects sex allocation in a parasitoid wasp Nasonia vitripennis

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Insect Science 2019年第5期26卷 853-862页

作者： marlene Chirault Christophe Bressac marlene Goubault Charlotte Lecureuil Institut de Recherche sur la Biologie de I'lnsecte UMR 7261 CNRS - Universite de ToursFaculte des Sciences et TechniquesParc GrandmontToursFrance

Insect reproduction is influenced by various external factors including temperature, a well-studied constraint. We investigated to what extent different levels of sperm limitation of males exposed to different heat stresses (34 and 36℃) afFect fem ales' offspring production and sex allocation in Nasonia vitripennis. In this haplodiploid parasitoid wasp attacking different species of pest flies, we investigated the effect of the quantity of sperm females received and stored in their spermatheca on their sperm use decisions, hence sex allocation, over successive ovipositions. In particular, we compared the sex allocation of females presenting three levels of sperm limitation (i.e.,mated with control, 34 ℃ heat-stressed or 36℃heat-stressed males) on each host they parasitized. To disentangle the potential reduction of sperm quality after a heat stress exposure from that of sperm quantity, we also explored the clutch size and sex ratio produced by fem ales that were partially sperm limited after copulating with multiply mated males. Independently of their sperm numbers, all types of fem ales produced a similar total number of offspring, but the more limited ones had fewer daughters. Sperm limitation further affected the distribution of daughters' production across *** addition to constraints acting on female physiology, male fertility should therefore be considered in studies measuring reproductive outputs of insects submitted to heat stresses.

关键词： heat stress multiple mating offspring production dynamic sex ratio sperm stock sperm use

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Do males choose their mates in the lekking moth Achroia grisella？ Influence of female body mass and male reproductive status on male mate choice

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Insect Science 2018年第5期25卷 861-868页

作者： marlene Goubault Rebecca Burlaud Institut de Recherche sur la Biologie de I'Insecte UMR 7261 CNRS-Universite Francois-Rabelais Tours France

Lekking males aggregate to attract females and contribute solely to egg fer- tilization, without any further parental care. Evolutionary theory therefore predicts them to be nonchoosy toward their mates, because any lost mating opportunities would out- weigh the benefits associated with such preferences. Nevertheless, due to time costs, the production of energetically costly sexual displays, and potential sperm limitation, the mat- ing effort of lekking males is often considerable. These factors, combined with the fact that many females of varying quality are likely to visit leks, could favor the evolution of male mate preferences. Here, we show that males of the lekking lesser wax moth, Achroia grisella, were indeed more likely to mate with heavier females in choice experiments, even at their virgin mating （i.e., when their reproductive resources have not yet been depleted by previous matings）. This differential female mating success could not be attributed to female behavior as heavy and light females showed similar motivation to mate （i.e., la- tency to approach the males） and time to copulate. Males seem to benefit from mating with heavier females, as fecundity positively correlated with female mass. This new em- pirical evidence shows that male mate choice may have been tmderestimated in lekking species

关键词： fecundity lek lesser wax moth mate choice mating status sexual behavior

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