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Analysis of human brain tissue derived from DBS surgery

Translational Neurodegeneration 2022年第1期11卷 625-640页

作者： Salla M.Kangas Jaakko Teppo Maija J.Lahtinen Anu Suoranta Bishwa Ghimire Pirkko Mattila Johanna Uusimaa markku varjosalo Jani Katisko Reetta Hinttala PEDEGO Research Unit University of OuluOuluFinland Medical Research Center Oulu University HospitalUniversity of OuluOuluFinland Biocenter Oulu University of OuluOuluFinland Institute of Biotechnology HiLIFE Helsinki Institute of Life ScienceUniversity of HelsinkiHelsinkiFinland Drug Research Program Faculty of PharmacyUniversity of HelsinkiHelsinkiFin-land Neurosurgery Research Unit of Clinical NeuroscienceOulu University HospitalUniversity of OuluOuluFinland Oulu Research Group of Advanced Surgical Technologies and Physics(ORGASTP) Research Unit of Clinical NeuroscienceOulu University HospitalUniversity of OuluOuluFinland Institute for Molecular Medicine Finland(FIMM) HiLIFE Helsinki Institute of Life ScienceUniversity of HelsinkiHelsinkiFinland Clinic for Children and Adolescents Division of Pediatric NeurologyOulu University HospitalOuluFinland

Background:Transcriptomic and proteomic profiling of human brain tissue is hindered by the availability of fresh samples from living *** samples usually represent the advanced disease stage of the ***-thermore,the postmortem interval can affect the transcriptomic and proteomic ***,fresh brain tissue samples from living patients represent a valuable resource of metabolically intact *** of deep brain stimulation(DBS)electrodes into the human brain is a neurosurgical treatment for,e.g.,movement ***,we describe an improved approach to collecting brain tissues from surgical instruments used in implantation of DBS device for transcriptomics and proteomics ***:Samples were extracted from guide tubes and recording electrodes used in routine DBS implantation procedure to treat patients with Parkinson’s disease,genetic dystonia and *** sequencing was performed in tissues extracted from the recording microelectrodes and liquid chromatography-mass spectrometry(LC-MS)per-formed in tissues from guide *** assess the performance of the current approach,the obtained datasets were compared with previously published datasets representing brain ***:Altogether,32,034 RNA transcripts representing the unique Ensembl gene identifiers were detected from eight samples representing both hemispheres of four *** using LC-MS,we identified 734 unique proteins from 31 samples collected from 14 *** datasets are available in the BioStudies database(accession number S-BSST667).Our results indicate that surgical instruments used in DBS installation retain brain material sufficient for protein and gene expression *** with previously published datasets obtained with similar approach proved the robustness and reproducibility of the ***:The instruments used during routine DBS surgery are a useful source for obtaining fresh brain tis-sues from living patien

关键词： Deep brain stimulation Movement disorders Brain Proteomics Transcriptomics RNA sequencing LC-MS Personalized medicine

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Genome-Wide Analysis of ETS Transcription Factor Binding Specificities,Target Genes and Regulatory Polymorphisms

Genome-Wide Analysis of ETS Transcription Factor Binding Spe...

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21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology

作者： Gong-Hong Wei Kimmo Palln Gwenael Badis Michael F.Berger Teemu Kivioja Martin Bonke Tilna Wahlfors Arttu Jolma markku varjosalo Andrew R.Gehrke Jian Yan Shaheynoor Talukder Ralner Lehtonen Outl Hallikas Mikko Tunnen Mikko Taipale Teuvo L.J.Tammela Laurl A.Aaltonen Hendrlk G.Stunnenberg Johanna Schleutker Timothy R.Hughes Martha L.Bulyk Esko Ukkonen Jussi Talpale THL University of Helsinki Finland University of Toronto Canada Harvard University USA University of Tampere Tampere Radboud University Nijmegen the Netherlands University of Tampere Tampere University Hospital Finland

＜正＞To comprehensively decipher the regulatory networks downstream of ETS transcription factors（TFs）,we systematically analyzed the binding specificities of all the human and mouse ETS family members,revealing the...

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