检索结果-南通市图书馆

Dietary supplementation of sulfur amino acids improves intestinal immunity to Eimeria in broilers treated with anti-interleukin-10 antibody

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2022年第3期10卷 382-389页

作者： Zhouzheng Ren Jiakun Yan Rose Whelan Xujie liao Daniel E.Bütz Maria K.Arendt mark E.Cook Xiaojun Yang Thomas D.Crenshaw College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxiChina Department of Animal Sciences University of WisconsinMadisonWIUSA Evonik Operations GmbH Hanau-WolfgangGermany

Oral antibody to interleukin-10(anti-IL-10)enhances the intestinal immune defense against *** sulfur amino acids methionine and cysteine(MtC)play essential roles in inducing and maintaining protective immune responses during intestinal ***,increased dietary MtC may support the anti-IL-10-induced intestinal immunity to ***(n=640)were arranged in a 2×2×2 factorial design with 2 levels of each of the 3 main factors:dietary standardized ileal digestible(SID)MtC levels(0.6%or 0.8%),dietary anti-IL-10 supplementation(with or without),and coccidiosis challenge(control or challenge).Briefly,the broilers were supplied with either 0.6%or 0.8%SID MtC,each with or without anti-IL-10(300 mg/kg),from d 10 to *** d 14,broilers from each diet were gavaged with either PBS or *** resulting Eimeria infection induced fecal oocyst shedding and intestinal *** fed 0.8%SID MtC(main effects,P≤0.05)had decreased feed-to-gain ratio,increased duodenum and cecum luminal anti-Eimeria IgA titers,and decreased fecal oocyst counts,when compared to 0.6%SID *** supplementation of anti-IL-10(main effects,P≤0.05)increased cecum luminal total IgA concentration and decreased cecum ***(P≤0.05)were detected for growth performance and cecum luminal IFN-γ.Briefly,the highest body weight gain and feed intake were reached in PBS-gavaged broilers fed 0.8%SID MtC with no anti-IL-10 and in Eimeria-challenged broilers fed 0.8%SID MtC with *** Eimeria-infected broilers,anti-IL-10 increased intestinal luminal IFN-γ and body weight gain only at 0.8%SID ***,anti-IL-10 increased intestinal luminal IFN-γ levels,decreased cecum lesions and restored growth only when fed with adequate amounts of sulfur amino *** findings underscore the importance of providing sufficient essential nutrients to support the anti-IL-10 induced immunity against coccidiosis.

关键词： Chicken Coccidiosis Egg yolk antibody Interleukin-10 Sulfur amino acid

Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2019年第2期10卷 390-401页

作者： Casey L.Durfey Sabrina E.Swistek Shengfa F.liao mark A.Crenshaw Henry J.Clemente Rooban V.K.G.Thirumalai Christy S.Steadman Peter L.Ryan Scott T.Willard Jean M.Feugang Department of Animal and Dairy Sciences Mississippi State UniversityMississippi StateMSUSA Department of Biochemistry Molecular BiologyEntomology and Plant PathologyMississippi StateMSUSA Clemente Associates MadisonCTUSA Institute of Imaging and Analytic Technology(I2AT) Mississippi State UniversityMississippi StateMSUSA Department of Pathobiology and Population Medicine Biochemistry Mississippi State UniversityMississippi StateMSUSA.

Background: Advances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles(MNP). Their use to detect and remove damaged spermatozoa from semen doses could be of great interest. Here, MNP were synthesized and tested for their ability to target apoptotic(annexin V) and acrosome-reacted(lectin) boar spermatozoa, for high-throughout retrieval in a magnetic field(nanoselection). The potential impacts of nanoselection on sperm functions and performance of offspring sired by sperm subjected to nanoselection were determined. Fresh harvested and extended boar semen was mixed with various amounts(0, 87.5, and 175 μg) of MNP-conjugates(Annexin V-MNP or Lectin-MNP) and incubated(10 to15 min) for 37 °C in Exp. 1. In Exp. 2, extended semen was mixed with optimal concentrations of MNP-conjugates and incubated(0, 30, 90, or 120 min). In Exp. 3, the synergistic effects of both MNP-conjugates(87.5 μg– 30 min)on spermatozoa was evaluated, followed by sperm fertility assessments through pregnancy of inseminated gilts and performance of neonatal offspring. Sperm motion, viability, and morphology characteristics were evaluated in all ***: Transmission electron microscopy, atomic force microscopy, and hyperspectral imaging techniques were used to confirm attachment of MNP-conjugates to damaged spermatozoa. The motility of nanoselected spermatozoa was improved(P 0.05).Conclusions: The findings revealed the benefit of magnetic nanoselection for high-throughput targeting of damaged sperm, for removal and rapid and effortles

关键词： Acrosome reaction Apoptosis Artificial insemination Boar Iron oxide nanoparticles Nanopurification Nanoselection Nanotechnology Reproduction Swine

Ecological Reenchantment： Interpretation on The Little Prince from the Perspective of Deep Ecology

在线全文

学校读者我要写书评

暂无评论

International English Education Research 2017年第1期 95-97页

作者： liao Zhihua University of Electronic Science and Technology of China Zhongshan Institute

Antoine de Saint-Exupe ry＇ s masterpiece The Little Prince directed by mark Osborne has been well-received by audience in China and beyond since its debut. This fantastic adaptation of movie explores the essence of life from children＇ s eye by adding Hollywood elements, criticizing the adult world characterized by hypocrisy, emptiness, indifference, obsession and alienation. That is how grow-ups live in crisis in their ecological spirit. This paper seeks to explore the reenchanting journey of the leading roles from the perspective of deep ecology, showcasing the necessity for human being to build a reenchanting world so as to promote the harmonious relationship between the people and nature.

关键词： reenchantment Little Prince deep ecology Little Girl

Realism in mark Twain's

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

三明学院学报 2000年第4期18卷 35-38页

作者： liao Fucai (Department of English Sanming Teachers’ College)

mark Twain was sole, incomparable, the Lincoln of American Literature. His novels demonstrate his preference realism to romanticism. by means of providing in them with his own experiences, regional stories and tales of the life of Americans. and of criticizing seriously those who lead a romantic life and those who insist on romanticism writing.

关键词： mark Twain redlism

基于星间测距和地面发射源的导航星座整网自主定轨

在线全文

学校读者我要写书评

暂无评论

国防科技大学学报 2009年第2期31卷 15-19,30页

作者：朱俊廖瑛文援兰国防科技大学航天与材料工程学院湖南长沙410073

导航卫星星座自主定轨技术是我国新一代卫星导航系统的关键技术之一。但是仅仅依靠星间相互测距定轨会因缺少地面基准而出现基准秩亏现象。针对这个问题,提出利用少量地面发射源随机工作的方式提供地面基准,将星间测距和地面发射源信息... 详细信息

导航卫星星座自主定轨技术是我国新一代卫星导航系统的关键技术之一。但是仅仅依靠星间相互测距定轨会因缺少地面基准而出现基准秩亏现象。针对这个问题,提出利用少量地面发射源随机工作的方式提供地面基准,将星间测距和地面发射源信息融合起来进行星座整网定轨,进一步提高定轨精度。最后利用仿真实验对该方法的合理有效性进行了验证。

关键词：导航系统自主定轨星间测量地面发射源

Construction of a microsatellite-based genetic linkage map for half-smooth tongue sole Cynoglossus semilaevis

在线全文

学校读者我要写书评

暂无评论

Current Zoology 2013年第1期59卷 99-108页

作者： Wentao SONG Guidong MIAO Yongwei ZHAO Yuze NIU Renyi PANG Xiaolin liao Changwei SHAO Songlin CHEN Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China College of Fisheries and Life Science Shanghai Ocean University Shanghai 201306 China

The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were developed with 567 mark- ers （565 microsatellite markers and two SCAR markers）. The parents and F1 progeny （92 individuals） were used as segregating populations. The female map was composed of 480 markers in 21 linkage groups, covering a total of 1388.1 cM, with an average interval 3.06 cM between markers. The male map consisted of 417 markers in 21 linkage groups, spanning 1480.9 cM, with an average interval of 3.75 cM. The female and male maps had 474 and 416 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1522.9 cM for females and 1649.1cM for males. Based on estimations of map length, the female and male maps covered 91.1% and 89.8% of the genome, respectively. Furthermore, two female-specific SCAR mark- ers, f-382 and f-783, were mapped on LG15f （linkage group 15 in female maps）. The present study presents a mid-density genetic linkage map for half-smooth tongue sole. These improved genetic linkage maps may facilitate systematic genome searches to identify quantitative trait loci （QTL）, such as disease resistance, growth and sex-related traits, and are very useful for marker-assisted selection breeding programs for economically important traits in half-smooth tongue sole [Current Zoology 59 （1）： 99-108, 2013].

关键词： Half-smooth tongue sole Cynoglossus semilaevis Microsatellite Genetic linkage map MAS

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于合成波长法的飞秒激光外差干涉测距方法

物理学报 2016年第14期65卷 35-41页

作者：廖磊易旺民杨再华吴冠豪清华大学精密仪器系精密测试技术及仪器国家重点实验室北京100084 北京卫星环境工程研究所北京100094

本文提出了一种基于合成波长法的飞秒激光外差干涉测距方法.系统采用两个带通滤波器产生两个具有一定波长差的单波长,用于产生合成波长.本方法结构简单,能量利用率高.与双频激光干涉仪在40 mm范围内的比对结果表明,该方法比对残差的标... 详细信息

本文提出了一种基于合成波长法的飞秒激光外差干涉测距方法.系统采用两个带通滤波器产生两个具有一定波长差的单波长,用于产生合成波长.本方法结构简单,能量利用率高.与双频激光干涉仪在40 mm范围内的比对结果表明,该方法比对残差的标准差为91 nm.

关键词：频率梳外差干涉绝对距离测量合成波长法

Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2021年第1期13卷 41-58页

作者： Xiangwen Peng Kaa Seng Lai Peilu She Junsu Kang Tingting Wang Guobao Li Yating Zhou jianjian Sun Daqing Jin Xiaolei Xu Lujian liao Jiandong Liu Ethan Lee Kenneth D.Poss Tao P.Zhong State Key Laboratory of Genetic Engineering School of Life SciencesZhong Shan HospitalFudan UniversityShanghai 200438China Shanghai Key Laboratory of Regulatory Biology Institute of Molecular MedicineEast China Normal University School of Life SciencesShanghai 200241China Department of Cell Biology Duke University Medical CenterDurhamNC 27710USA Department of Biochemistry and Molecular Biology Mayo ClinicRochesterMN 55905USA Department of Pathology and Laboratory Medicine McAllister Heart InstituteUniversity of North Carolina at Chapel HillChapel HillNC 27599USA Department of Developmental and Cell Biology Vanderbilt University School of MedicineNashvilleTN 37232USA T These authors share first authorship.

Heart regeneration occurs by dedifferentiation and proliferation of pre-existing cardiomyocytes(CMs).However,the signaling mechanisms by which injury induces CM renewal remain incompletely ***,we find that cardiac injury in zebrafish induces expression of the secreted Wnt inhibitors,including Dickkopf 1(Dkkl),Dkk3,secreted Frizzled-related protein 1(sFrpl),and sFrp2,in cardiac tissue adjacent to injury *** blocking of Wnt activity via Dkkl overexpression enhances CM proliferation and heart regeneration,whereas ectopic activation of Wnt8 signaling blunts injury-induced CM dedifferentiation and *** Wnt signaling is dampened upon injury,the cytoplasmic β-catenin is unexpectedly increased at disarrayed CM sarcomeres in myocardial wound *** analyses indicated that p21-activated kinase 2(Pak2)is induced at regenerating CMs,where it phosphorylates cytoplasmic β-catenin at Ser 675 and increases its stability at disassembled ***-specific induction of the phospho-mimeticβ-catenin(S675E)enhances CM dedifferentiation and sarcomere disassembly in response to ***,inactivation of Pak2 kinase activity reduces the Ser 675-phosphorylatedβ-catenin(pS675-β-catenin)and attenuates CM sarcomere disorganization and dedifferentiation・Taken together,these findings demonstrate that coordination of Wnt signaling inhibition and Pak2/pS675-βYatenin signaling enhances zebrafish heart regeneration by supporting CM dedifferentiation and proliferation.

关键词： heart regeneration Wnt signaling PAK2 kinase cardiomyocyte proliferation cardiomyocyte dedifferentiation zebrafish

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

高内表面质量铝合金矩形管拉拔成形工艺研究

中国机械工程 2007年第18期18卷 2252-2256页

作者：周杰宋立群廖旭重庆大学重庆400044

为了研究矩形管拉拔成形工艺，采用有限元分析软件Deform-3D建立了铝合金圆形管拉拔成矩形管的有限元分析模型，并对拉拔工艺的整个过程进行了模拟分析，获得了拉拔过程中起皱缺陷的形成机理，为此，经过多次有限元方法（finite element... 详细信息

为了研究矩形管拉拔成形工艺，采用有限元分析软件Deform-3D建立了铝合金圆形管拉拔成矩形管的有限元分析模型，并对拉拔工艺的整个过程进行了模拟分析，获得了拉拔过程中起皱缺陷的形成机理，为此，经过多次有限元方法（finite element method，FEM）分析，找到了合理过渡矩形管件形状及模孔锥角的最佳范围为10°～12°。根据铝合金矩形管产品对内表面质量无拉痕的要求，进一步调整了相关工艺参数，择优后过渡矩形的长弧边高度为5mm，短弧边高度为3mm，最佳模孔锥角为11°。得到了拉拔力与模孔锥度之间的关系曲线。研究成果对矩形管的实际生产具有指导作用。

关键词：矩形铝合金管拉拔模拟仿真有限元法