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Electro-physical Properties of Super-thin Basalt Fiber Chemically Modified by Hydrochloric or Sulphuric Acid

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Journal of Modern Physics 2011年第12期2卷 1450-1454页

作者： Sergey K. Nikoghosyan Aram A. Sahakyan vasak B. Gavalyan vachaganv. Harutyunyan Aghasi S. Hovanisyan Hrant.N. Yeritsyan vovik. A. Atoyan Konstantin I. Puskulyan mark Gerchikov Narek v. Hakobyan Artur v. Hovhannisyan 不详

The influence of hydrochloric or sulphuric acid treatment on the electro-physical properties of superthin basalt fiber (STBF) made from Armenian basalt rocks was studied. Specific electric resistance for direct and alternating currents, dielectric parameters of ε, and ε,, were measured. It is shown that specific resistance and dielectric parameters of super-thin basalt fiber change essentially after hydrochloric or sulphuric acid treatment. The temperature dependences of these parameters were studied, too, and their non–monotonic behavior was observed. The probable variation of mentioned STBF parameters is explained by different water absorption capacity of pores as a result of acid treatment.

关键词： super-thin basalt fiber water molecule hydrochloric and sulphuric acid treatment dipole-relaxation.

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隐形微球表面吸附血浆蛋白的蛋白质组学分析

中国科学：生命科学 2012年第3期42卷 185-195页

作者： Yevgeniya Le 李丽王敦成 mark D.Scott Canadian Blood Services VancouverBCV6T 1Z3Canada Centre for Blood Research University of British ColumbiaVancouverBCV6T 1Z3Canada Department of Pathology and Laboratory Medicine University of British ColumbiaVancouverBCV6T 1Z3 Canada

细胞和生物材料表面的甲氧基聚乙二醇(mPEG)修饰是一项具有广阔应用前景的非药理性免疫调节技术,但由于细胞的不稳定性,目前对其表面与血浆间相互作用的认识还不够深入.本实验采用相对稳定的乳胶微球作为模型,来研究其表面对血浆蛋白的... 详细信息

细胞和生物材料表面的甲氧基聚乙二醇(mPEG)修饰是一项具有广阔应用前景的非药理性免疫调节技术,但由于细胞的不稳定性,目前对其表面与血浆间相互作用的认识还不够深入.本实验采用相对稳定的乳胶微球作为模型,来研究其表面对血浆蛋白的吸附.结果显示,血浆总蛋白的吸附水平随着mPEG修饰浓度和分子量的增加而降低,未修饰的乳胶微球表面蛋白吸附量为(159.9±6.4)ng/cm2,mPEG修饰后,蛋白吸附量分别下降至(18.4±0.8)ng/cm2(1mmol/L2kDSCmPEG)和(52.3±5.3)ng/cm2(1mmol/L20kDSCmPEG).聚炳烯酰胺凝胶电泳(SDS-PAGE)和同位素标记相对和绝对定量的质谱技术(iTRAQ-MS)分析表明,在PEG修饰微球表面的蛋白质吸附层中,参与免疫系统活化的蛋白质丰度明显下降.因此,免疫修饰细胞和材料的生物学效应是通过降低抗原性及减弱表面与大分子间相互作用来实现的.

关键词：聚乙二醇修饰免疫修饰同位素标记相对和绝对定量技术质谱分析聚苯乙烯乳胶微球蛋白质组学

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土壤微生物多样性研究的新方法

土壤学报 2004年第1期41卷 103-107页

作者：马万里 Josquin Tibbits mark Adams 内蒙古师范大学生物系 Forest Science Center University of MelboumeCreswick

传统的分离培养和鉴定土壤微生物方法所具有的困难性和局限性 ,是造成难以深入了解土壤微生物生态学特性和多样性组成方面的主要障碍。本文运用分子生物学技术 ,以澳大利亚两种主要森林类型的土壤微生物多样性研究为实例 ,介绍了从土壤... 详细信息

传统的分离培养和鉴定土壤微生物方法所具有的困难性和局限性 ,是造成难以深入了解土壤微生物生态学特性和多样性组成方面的主要障碍。本文运用分子生物学技术 ,以澳大利亚两种主要森林类型的土壤微生物多样性研究为实例 ,介绍了从土壤中直接提取土壤微生物DNA的方法以及末端限制性酶切片段长度多态性 (T RFLP)分析的基本原理和方法。作者认为 ,用该方法提取的土壤真菌DNA的纯度高 ,完全适合PCR扩增和T RFLP分析的要求。T

关键词：土壤微生物多样性 16S核糖体DNA 真菌核糖体DNA ITS T-RFLP分析

NMR Imaging of Heavy Crude Oil for Softening Detection under Pleat Treatment

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材料科学与工程（中英文A版） 2011年第4X期1卷 545-551页

作者： Morozov Evgeny v. Martyanov Oleg N. volkov Nikita v Falaleev Oleg v. Kirensky Institute of Physics SB RAS Krasnoyarsk 660036 Russia Special designing and technological bureau "Nauka" KSC SB RAS Krasnoyarsk 660049 Russia Boreskov Institute of Catalysis SB RAS Novosibirsk 630090 Russia

N-烷基嘧啶DNA糖基化酶腺病毒提高人骨肉瘤化疗敏感性的研究

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中华病理学杂志 2006年第6期35卷 352-356页

作者：王东仲召阳张沁宏李增鹏 mark R.Kelley 第三军医大学大坪医院野战外科研究所肿瘤中心第三军医大学大坪医院病理科 Herman B.Well Center for Pediatric Research Indiana University School of MedicineIndianapolisIndiana 46202USA

目的构建复制缺陷型腺病毒N-烷基嘧啶DNA糖基化酶(MPG)表达载体,观察人骨肉瘤细胞HOS过表达MPG及其对DNA损伤药物甲基甲磺酸酯(MMS)、N-甲基-N1-硝基-N-亚硝基鸟嘌呤(MNNG)和替莫唑胺(TMZ)敏感性的影响。方法应用流式细胞术、蛋白印迹和... 详细信息

目的构建复制缺陷型腺病毒N-烷基嘧啶DNA糖基化酶(MPG)表达载体,观察人骨肉瘤细胞HOS过表达MPG及其对DNA损伤药物甲基甲磺酸酯(MMS)、N-甲基-N1-硝基-N-亚硝基鸟嘌呤(MNNG)和替莫唑胺(TMZ)敏感性的影响。方法应用流式细胞术、蛋白印迹和HEX标记寡核苷酸方法确定MPG腺病毒表达载体Ad5HA-MPG在人体骨肉瘤细胞HOS中的感染效率、MPG蛋白表达和MPG酶活性;MTS、硫氰酸盐B法和[3H]胸腺嘧啶掺入法检测经MMS(1、2μmol/L)、MNNG(25、50μmol/L)和TMZ(1、2mmol/L)处理过的转染细胞存活情况;PE-Annexinv/7-AAD流式细胞术检测细胞凋亡。结果10个感染增殖指数(MOI)的Ad5HA-MPG可使90%以上HOS感染,感染细胞高表达MPG并具有MPG酶活性。MPG过表达HOS显著地提高DNA损伤性化疗药物MMS、MNNG和TMZ的敏感性,其半数抑制浓度(IC50)值分别下降了6·0、4·5和2·5倍。结论Ad5HA-MPG瞬时MPG过表达,可能是一种提高骨肉瘤细胞对DNA损伤性化疗药物敏感性的潜在治疗方法。

关键词：骨肿瘤腺病毒感染抗肿瘤药

The Success Story of Capt. Angelex A. Panes on Board the M/v SKS Tiete

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Journal of Shipping and Ocean Engineering 2016年第3期6卷 149-153页

作者： Brian Gil S. Sarinas[ mark Eden G. Ellaga Officer-in-Charge Research Office and Researcher John B. Lacson Foundation Maritime University-ArevaloSto. Niho SurArevalo lloilo City Republic of the Philippines Alumnus John B. Lacson Foundation Maritime University-Arevalo and 3rd Officer M/V SKS Tiete

Few studies have been conducted on success stories of JBLFMU-Arevalo alumni. Thus, this study aimed to determine the success story of Capt. Angelex Arevalo Panes, an alumnus of the then, John B. Lacson Colleges Foundation （Arevalo）, Inc. of Batch 1995 now, John B. Lacson Foundation Maritime University-Arevalo. The story of Capt. Panes tells his family and educational background, career paths, driving force to work efficiently, hardwork, coping strategies, job encounters, edged as first Filipino captain of M/v SKS Tiete, skills as effective team leader, managerial techniques in resolving conflicts under watch, problems and solutions encountered on board, preparations for his sunset life, and his message to all aspiring seafarers. This paper gives glimpse to the life of Philippine＇s modem hero-a seafarer like Capt. Angelex A. Panes and lessons to those who would become seafarers.

关键词： JBLFMU-Arevalo alumnus seafaring success story of seafarer.

Microstructural Modeling of Thermally-Driven β Grain Growth, Lamellae &Martensite in Ti-6Al-4v

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Modeling and Numerical Simulation of Material Science 2020年第3期10卷 55-73页

作者： Matteo villa Jeffery W. Brooks Richard P. Turner mark Ward School of Metallurgy & Materials University of Birmingham Birmingham UK

The microstructural kinetics of β grain growth in the β field of a Ti-6Al-4v alloy was studied by a series of controlled heat treatments at constant temperature rates. Heating rates of 5°C/s, 50°C/s and 500°C/s were considered, stopping at different peak temperatures. The thickness evolution of martensitic needles and lamellar α laths, formed on cooling, was also investigated, by soaking the material above its β-transus temperature and cooling down at 5°C/s, 50°C/s, 100°C/s and 300°C/s till ambient temperature. Quantitative microstructural analyses were used to measure the particle dimensions. The β grain growth kinetics was reasonably well described by a modified Avrami equation. The thickness of α lamellae was a function of the cooling rate and the β grain dimension in which they nucleated. The martensite needle thickness was shown to be a function of the cooling rate to which the material was subjected.

关键词： Titanium Welding Rapid Heating Phase Alpha Beta

HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis

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Cellular & Molecular Immunology 2016年第4期13卷 451-461页

作者： Susanne Wolkerstorfer Elisabeth Schwaiger mark Rinnerthaler Iris Karina Gratz Thomas Zoegg Hans Brandstetter Gertrude Achatz-Straussberger Department of Molecular Biology University of Salzburg Salzburg Austria Department of Cell Biology University of Salzburg Salzburg Austria Department of Dermatology University of California San Francisco CA USA Department of Dermatology Division of Molecular Dermatology and EB House Austria Paracelsus Medical University Salzburg Austria.

Deletion of HAX1 in mice causes a severe reduction in the numbers of lymphocytes in the bone marrow and in the spleen. Additionally, B220＋ B progenitor cells in the bone marrow are reduced, suggesting an important function of HAX1 in B cell development. HAX1 is thought to play a protective role in apoptotic processes; therefore, we investigated the role of HAX1 in bone marrow B progenitor cells and splenic B cells. We did not observe an effect on the survival of Hax1-/- bone marrow cells but detected enhanced survival of splenic Hax1-/- B cells upon in vitro starvation/ growth-factor withdrawal. To explain this apparent inconsistency with previous reports of HAX1 function, we also studied the B cell receptor （BCR）-induced apoptosis of IgM-stimulated splenic naive B cells and found that apoptosis decreased in these cells. We further found impaired internalization of the BCR from Hax1-/- splenic B cells after IgM crosslinking; this impaired internalization may result in decreased BCR signaling and, consequently, decreased BCR-mediated apoptosis. We measured HAX1 binding to the cytoplasmic domains of different Ig subtypes and identified KvKWI（v）F as the putative binding motif for HAX1 within the cytoplasmic domains. Because this motif can be found in almost all Ig subtypes, it is likely that HAX1 plays a general role in BCR-mediated internalization events and BCR-mediated apoptosis.

关键词： viability apoptosis internalization BCR surface plasmon resonance analysis (SPRA) wild type (WT) B cellreceptor (BCR) co-immunoprecipitation (co-IP) room temperature (RT) annexin v(A) eFluor (eF)

Anatomy of Free Flap Failures: Dissection of a Series

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Modern Plastic Surgery 2013年第3期3卷 89-95页

作者： Steven P. Davison mark W. Clemens Andrew L. Kochuba Department of Plastic Surgery Georgetown University Hospital Washington DC USA

Introduction: Free flap success rates have remained stable in recent years ranging 93% to 98%. Historically, the causes of free flap failures were attributed to the surgeon’s inexperience and technique. However, there are factors beyond the surgical anastomosis that contribute to flap failure. The purpose of this study is to review each case of total flap loss in detail to develop a better understanding of complications. Methods: A retrospective study was performed over eleven years in a single surgeon’s practice, a predominantly head and neck reconstructive practice. All charts were independently reviewed. In patients who sustained total flap loss, a review was conducted of patient comorbidites, anesthesia records, perioperative and follow-up notes. Results: A total of 514 free flaps were performed. 76% (392) of these flaps were for head and neck reconstruction. There were 22 total flap losses (4%) and 26 partial flap losses (5%). Of the 22 total flap losses, four flaps were avulsed, five flaps were in patients later found to have coagulation disorders (homozygous mutations of the MTHFR gene and factor v Leiden), four patients were exposed to neosynephrine, two patients remained hypotensive perioperatively, and four delayed flap losses were attributed to pseudomonal infection. Five losses were technical or related to flap inexperience. Several representative case scenarios are illustrated. Conclusion: Careful review of free flap failures indicates that a thorough workup (particularly coagulation disorders), flap selection, surgeon to anesthesia communication, proper securing of the flap, and postoperative patient blood pressure and infection control have a greater part to play in this new era of anastomotic success.

关键词： Free Flaps Flap Loss Head and Neck Reconstruction Coagulopathy MTHFR Factor v Leiden