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PGR5-PGRL1-Dependent Cyclic Electron Transport Modulates Linear Electron Transport Rate in Arabidopsis thaliana

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Molecular Plant 2016年第2期9卷 271-288页

作者： marjaana suorsa Fabio Rossi Luca Tadini Mathias Labs Monica Colombo Peter Jahns Martin M. Kater Dario Leister Giovanni Finazzi Eva-Mari Aro Roberto Barbato Paolo Pesaresi Molecular Plant Biology Department of Biochemistry University of Turku 20014 Turku Finland Dipartimento di Bioscienze Unlversit~ degli studi di Milano 20133 Milano Italy Plant Molecular Biology (Botany) Department Biology I Ludwig-Maximilians-Universit~t MSnchen 82152 Planegg-Martinsded Germany Centro Ricerca e Innovazione Fondazione Edmund Mach 38010 San Michele all'Adige Italy Plant Biochemistry Heinrich-Heine-University Dusseldorf Universitstsstrasse 140225 Dusseldorf Germany Laboratoire de Physiologie Cellulaire & Vegetale Unite Mixte de Recherche 5168 Centre National de la Recherche Scientifique 38054 Grenoble France Dipartimento di Scienze dell'Ambiente e della Vita Universita del Piemonte Orientale viale Teresa Michel 11 15121 Alessandria Italy These authors contributed equally to this article

Plants need tight regulation of photosynthetic electron transport for survival and growth under environ- mental and metabolic conditions. For this purpose, the linear electron transport （LET） pathway is supple- mented by a number of alternative electron transfer pathways and valves. In Arabidopsis, cyclic electron transport （CET） around photosystem I （PSI）, which recycles electrons from ferrodoxin to plastoquinone, is the most investigated alternative route. However, the interdependence of LET and CET and the relative importance of CET remain unclear, largely due to the difficulties in precise assessment of the contribution of CET in the presence of LET, which dominates electron flow under physiological conditions. We there- fore generated Arabidopsis mutants with a minimal water-splitting activity, and thus a low rate of LET, by combining knockout mutations in Psb01, PsbP2, PsbQ1, PsbQ2, and PsbR loci. The resulting 45 mutant is viable, although mature leaves contain only ~20% of wild-type naturally less abundant Psb02 protein. 45 plants compensate for the reduction in LET by increasing the rate of CET, and inducing a strong non-photochemical quenching （NPQ） response during dark-to-light transitions. To identify the molecular origin of such a high-capacity CET, we constructed three sextuple mutants lacking the qE component of NPQ （45 npq4-1）, NDH-mediated CET （45 crr4-3）, or PGR5-PGRLl-mediated CET （45 pgrS）. Their analysis revealed that PGR5-PGRLl-mediated CET plays a major role in ~pH formation and induction of NPQ in C3 plants. Moreover, while pgr5 dies at the seedling stage under fluctuating light conditions, 45 pgr5 plants are able to survive, which underlines the importance of PGR5 in modulating the intersystem electron transfer.

关键词： Arabidopsis Photosynthesis Linear Electron Transport Cyclic Electron Transport Oxygen EvolvingComplex PGR5

Chloroplast-targeted J-domain proteins-Expression and role of AtJ8, AtJ11 and AtJ20 in Arabidopsis thaliana

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Chloroplast-targeted J-domain proteins-Expression and role o...

The effect of inactivation of aldehyde dehydrogenase on pheromone production by a gut bacterium of an invasive bark beetle,Dendroctonus valens

第九届全国植物结构与生殖生物学学术研讨会

作者： Mirva Piippo marjaana suorsa Markus Nurmi Eveliina Aro Eva-Mari Aro Department of Biology University of Turku

DnaJ type of chaperones is a large family of proteins found in all life kingdoms. Our database searches revealed that Arabidopsis chloroplasts may contain as many as 26 different J-domain proteins and these chloroplast-targeted J-domain proteins can be roughly divided into two distinct groups according to the gene expression profiles from a number of experimental conditions. Both informations from clustering of gene express profiles and asignment of domain compositions showed that the J-domain proteins may have diverse roles both in chloroplast activity and in stress responses, possiblely functioning as chaperones or/and co-chaperones. The expression of one of the chloroplast-targeted J-domain protein AT1G80920 (AtJ8) that harbors only the J-domain, is negatively regulated both at transcript and protein levels by light and signals derived from photosynthesis such as sucrose and glucose. Further studies showed that the AtJ8 protein is absent in light due to transcriptional downregulation, and although present in darkness the protein undergoes rapid turnover. The responsiveness of the expression of AtJ8 to sugars and light/dark treatments suggests that the expression of this protein, is regulated by photosynthesis-derived signals but is also tightly connected to the presence of FTSH proteases. For getting further insight of J-domain proteins in the chloroplast, the role of three small chloroplast-targeted J-domain proteins, AtJ8 (At1g80920), AtJ11 (At4g36040) and AtJ20 (At4g13830), was investigated using knock-out mutants of Arabidopsis thaliana. Photochemical efficiency, capacity of CO2 assimilation, stabilization of Photosystem (PS) II dimers and supercomplexes under high light illumination, energy distribution between PSI and PSII and phosphorylation of PSII-LHCII proteins, global gene expression profiles and oxidative stress responses of these J-domain protein mutants were analyzed. The results obtained strongly indicated that knockout of one of these proteins cau

关键词： Chaperone J-domain protein CO2 fixation photosystem II thylakoid protein phosphorylation stress response reactive oxygen species

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Insect Science 2023年第2期30卷 459-472页

作者： Qingjie Cao Tuuli-marjaana Koski Huiping Li Chi Zhang Jianghua Sun College of Forestry Hebei Agricultural UniversityBaodingHebei ProvinceChina College of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaodingHebei ProvinceChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina Rural Energy and Environment Agency Ministry of AgricultureandRural AffairsBeijingChina

Semiochemical-based management strategies are important for controlling bark beetles,such as invasive Red Turpentine Beetle(Denroctonus valens),the causal agent for mass mortality of pine trees(Pinus spp.)in *** has been previously shown that the pheromone verbenone regulates the attack density of this beetle in a dose-dependent manner and that the gut bacteria of *** are involved in verbenone ***,molecular functional verification of the role of gut bacteria in the pheromone production of *** is still *** better understand the molecular function of gut bacterial verbenone production,we chose a facultative anaerobic gut bacterium(Enterobacter xiangfangensis)of *** based on its strong ability to convert cis-verbenol to verbenone,as shown in our previous study,and investigated its transcriptomics in the presence or absence of cis-verbenol under anaerobic conditions(simulating the anoxic environment in the beetle's gut).Based on this transcriptome analysis,aldehyde dehydrogenase(ALDH1)was identified as a putative key gene responsible for verbenone production and was knocked-down by homologous recombination to obtain a mutant *** *** results show that these mutants had significantly decreased the ability to convert the monoterpene precursor to verbenone compared with the wild-type bacteria,indicating that ALDH1 is primarily responsible for verbenone conversion for this bacterium *** findings provide further mechanistic evidence of bacterially mediated pheromone production by ***,add new perspective for functional studies of gut bacteria in general,and may aid the development of new gene silencing-based pest management strategies.

关键词： aldehyde dehydrogenase Dendroctonus valens gut bacteria mutants transcriptome verbenone

Effects of simulated gut pH environment on bacterial composition and pheromone production of Dendroctonus valens

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Insect Science 2024年第1期31卷 225-235页

作者： Qingjie Cao Yu Zhao Tuuli-marjaana Koski Huiping Li Jianghua Sun College of Forestry Hebei Agricultural UniversityBaodingHebei ProvinceChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina College of Life Science/Hebei Basic Science Center for Biotic Interactions Institute of Life Science and Green DevelopmentHebei UniversityBaodingHebei ProvinceChina

Bark beetles are an economically and ecologically important insect group,with aggregation behavior and thus host colonization success depends on pheromone-mediated *** some species,such as the major invasive forest pest in China,red turpentine beetle(Dendroctonus valens),gut microbiota participates in pheromone production by converting tree monoterpenes into pheromone ***,how variation in gut microenvironment,such as pH,affects the gut microbial composition,and consequently pheromone production,is *** this study,we fed wild caught *** with 3 different pH media(main host diet with natural pH of 4.7;a mildly acidic diet with pH 6 mimicking the beetle gut pH;and highly acidic diet with pH 4),and measured their effects on the gut pH,bacterial community and production of the main aggregation and anti-aggregation pheromone(verbenone).We further tested the verbenone production capacity of 2 gut bacterial isolates in different pH environments(pH 6 and 4).Compared to natural state or main host diet,feeding on less acidic diet(pH 6)diluted the acidity of the gut,whereas feeding on highly acidic diet(pH 4)enhanced *** changes in gut pH reduced the abundance of dominant bacterial genera,resulting in decreased verbenone ***,the highest pheromone conversion rate of the bacterial isolates was observed in pH mimicking the acidity in beetle *** together,these results indicate that changes in gut pH can affect gut microbiota composition and pheromone production,and may therefore have the potential to affect host colonization behavior.

关键词： Dendroctonus valens gut bacteria gut pH verbenone

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千湖万树和疯狂的桑拿

环球人文地理 2018年第11期 14-23页

作者：刘容徐丹蕾陈心杰 LuDi_ 沈海滨 Jaakko Thti Mikko Nikkinen Elina Manninen Keksit Aapo Laiho Jukka Heinovirta Stock Riekkinen Harri Tarvainen marjaana Tasala Juho Kuva 芬兰国家旅游局

蓝色、白色和绿色,是芬兰的主色调。蓝色是天空和湖泊营造的静谧,白色是漫长冬日里的简洁与肃穆,绿色则是无垠森林透出的清新。无处不在的自然气息,让芬兰人与生俱来一种质朴的气质,他们热爱自然、亲近自然,迷恋上了那种简单而纯粹的美... 详细信息

蓝色、白色和绿色,是芬兰的主色调。蓝色是天空和湖泊营造的静谧,白色是漫长冬日里的简洁与肃穆,绿色则是无垠森林透出的清新。无处不在的自然气息,让芬兰人与生俱来一种质朴的气质,他们热爱自然、亲近自然,迷恋上了那种简单而纯粹的美好。大自然恨不得把所有绝妙的景致都集中在芬兰,于是成就了"全球最幸福国家"的绝美景致。这里号称"千湖之国",春夏秋冬始终不变的乐趣是水与

关键词：芬兰人亲近自然水上运动千湖之国