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圈养猞猁多雄交配雄性的成功繁殖(英文)

动物学报 2007年第3期53卷 408-416页

作者： Sergey NAIDENKO mariya EROFEEVA Frank GOERITZ Katrin NEUBAUER Joerns FICKEL Katarina JEWGENOW A.N. Severtsov Institute of Ecology and Evolution RAS Leninsky pr. 33 Moscow 119071 Russia Leibniz-Institute for Zoo- and Wildlife Research Alfred-Kowalke-Str. 17 D-10315 Berlin Germany

研究了笼养猞猁多雄交配的结果以及雄性的成功繁殖。雄兽与发情雌兽随机配对,其繁殖成功(所产生的后代数量)不取决于与雌兽的交配顺序、交配次数与持续时间、遗传关系或雄兽的行为特征,但似乎取决于精子质量,特别是取决于形态正常精子... 详细信息

研究了笼养猞猁多雄交配的结果以及雄性的成功繁殖。雄兽与发情雌兽随机配对,其繁殖成功(所产生的后代数量)不取决于与雌兽的交配顺序、交配次数与持续时间、遗传关系或雄兽的行为特征,但似乎取决于精子质量,特别是取决于形态正常精子的百分率。繁殖成功的雄兽比其竞争对手具有更多的形态正常的精子,似乎更能成功地诱发交配雌兽的排卵。证实了4窝(占总窝数的20%)幼兽的双重父亲身份。在所有情形下, 2雄1雌的交配间隔为24h 。

关键词：精子竞争繁殖畸形精子交配行为

Quantum-Chemical Estimating Interaction of <i>sp<sup>2</sup></i>-Carbon Nanoclusters with PE and PP Oligomers

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Journal of Materials Science and Chemical Engineering 2019年第7期7卷 1-11页

作者： Mykola Kartel Euvgeniy Demianenko Оksana Сherniuk mariya Terets Yurii Sementsov Bo Wang Transportation and Logistics College Ningbo University of Technology Ningbo China Department of Nanoporous and Nanosized Carbon Materials O. Chuiko Institute of Surface Chemistry NASU Kyiv Ukraine

The purpose of this work was to examine the interaction of graphene-like nanoclusters with fragments of polymers of the same nature, but somewhat different structure, for example, polyethylene (PE) and polypropylene (PP) by means of quantum chemistry. By method of density functional theory with the exchange-correlation functional B3LYP, the basis set 6 - 31 G (d, p) and the Grimme’s dispersion correction, the energy values have been calculated of interaction between nanocarbon fragments and oligomers of PE and PP, the most probable structures of their intermolecular complexes being optimized. A graphene-like plane of 40 carbon atoms and 16 atoms of hydrogen was chosen as a model for the surface of the graphene and carbon nanotubes (CNT). In order to take into account the dimensional effect of the surface of the nanotube fragment model on the interaction energy, in addition to the above described, two larger models were used, with the general formula C54H18 and C96N24. It has been found that the interaction energy of nanocarbon fragment with an oligomer of PP is greater, compared with PE, which is consistent with the experimental data on melting temperatures of pure polymers and nanotube-polymer composites. The polymer with a surface of nanocarbon fragment forms an intermolecular complex not bound covalently and retained by intermolecular dispersion forces. Oligomers of polymeric matters and carbon surfaces in formed nanocomplex are placed closer to each other than separate polymeric links between them.

关键词： Nanocomposite Carbon Nanotube Polyethylene Polypropylene Density Functional Theory Method Cluster Approximation Dispersion Forces of Interaction

Thermo-and pH-sensitive Polymer with Pendant Spacer-linked Imidazole Cycles

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Chinese Journal of Polymer Science 2024年第4期42卷 437-445页

作者： Natalya V.Zakharova Stanislav N.Zelinskiy mariya S.Strelova Elena N.Danilovtseva Vadim V.Annenkov Institute of Macromolecular Compounds of the Russian Academy of Sciences Saint Petersburg199004Russia Limnological Institute of Siberian Branch of the Russian Academy of Sciences Irkutsk664033Russia

By the reaction of poly(acryloyl chloride) with N-(3-aminopropyl)imidazole, poly(N-(3-(1H-imidazol-1-yl)propyl)acrylamide) was synthesized. The new polymer contains an imidazole ring removed from the main chain by a spacer of five bonds. The structure and purity, molecular weight, hydrodynamic and thermosensitive properties of the obtained sample were studied by1H-and13C-NMR, FTIR spectroscopy, acid-base titration, light scattering, turbidimetry and viscometry. The observed ability of the imidazole-containing polymer to form and destroy associates in water-salt solutions at pH 6.6-7.4 and temperatures of 29-48℃ indicates that these are promising candidates for designing complex biomedical systems. The new polymer is able to form complexes with oligo-DNA more actively than poly(1-vinylimidazole), which is of interest for gene delivery applications. The polymer cross-linked with epichlorohydrin gives micro-relief coatings on the plastic surface, and the modified surface is able to attach negatively charged objects. This thermo-and pH-sensitive polymer modification can be applied to create finely controlled surfaces for cell culturing.

关键词： Imidazole-containing polymer Thermolability pH-sensitivity The modified surface DNA immobilization

Allelopathy of Cold Water Extracts from Origanum vulgare ssp. vulgare L.

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Journal of Agricultural Chemistry and Environment 2014年第4期3卷 144-150页

作者： Asya Pencheva Dragoeva Vanya Petrova Koleva Zheni Dimitrova Nanova mariya Zhivkova Kaschieva Faculty of Natural Sciences University of Shumen Shumen Bulgaria

Secondary metabolites in medicinal plants could lead to discovery of new classes of herbicides. Recently aromatic plants have gained interest as a source of allelopathic secondary metabolites. Origanum vulgare ssp. vulgare L. infusions in hot water are used in folk medicine and possess proved beneficial biological activity. Plant-to-plant variability of metabolites due to genetic heterogeneity is established in Lamiaceae family. From this point of view, studies on plants from different geographic regions might reveal important sources of variability. The objective of this study was to evaluate allelopathic activity of cold water extracts made from the aerial parts of O. vulgare ssp. vulgare growing wild in Northeast Bulgaria in laboratory conditions. The allelopathic effect was evaluated using root elongation test and Allium cepa-test. Oregano extracts (17.5 g/l, 52.5 g/l) significantly decreased root length of Triticum aestivum L. (P ≤ 0.001). The root growth reduction could serve as a sign for presence of water soluble allelopathic secondary metabolites in the plant tested. Oregano (3.5 g/l) inhibited cell division in Allium root meristematic cells. The decline of the mitotic index indicates the occurrence of a cytotoxic effect. Oregano induced abnormalities in mitotic and interphase cells, so can be also considered as genotoxic. The observed macroscopic and microscopic effects of tested extracts indicated presence of water soluble allelochemicals in O. vulgare ssp. vulgare. This characteristic could be further studied as a possibility to be used in weed management programs.

关键词： Origanum vulgare ssp. vulgare L. Allelopathy Root Growth Inhibition Allium cepa-Test

Nanometallocarbosilanes： Synthesis, Physicochemical Properties and Structure

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Journal of Chemistry and Chemical Engineering 2014年第3期8卷 232-242页

作者： Galina Shcherbakova Pavel Storozhenko mariya Blokhina Valery Shatunov Denis Sidorov DmitrySidorov Gleb Yurkov SSC RF Federal State Unitary Enterprise "State Research Institute for Chemistry and Technology of Organoelement Compounds " Moscow 105118 Russia A.A. Baikov Institute for Metallurgy and Material Science of Russian Academy of Science Moscow 119991 Russia

Highly efficient synthesis methods have been developed and characteristics of nanometallocarbosilanes molecular structure were studied by the research team of GNIIChTEOS （State Research Institute for Chemistry and Technology of Organoelement Compounds）. Nanometallocarbosilanes were synthesized by thermal co-condensation of oligocarbosilanes and alkyl amides of refractory metals. Initial, intermediate and final products of side reactions were characterized by 1H, 13C, 29Si NMR （nuclear magnetic resonance）, IR （infra-red） spectroscopy, GPC （gel-penetrating chromatography）, TGA （thermal gravimetric analysis）, TEM （transmission electron microscopy）, SEM （scanning electron microscopy）, RES （X-ray phase analysis） and elemental analysis. The proposed synthesis method of nanometallocarbosilanes was lbund to produce fusable soluble organosilicon oligomers with homogeneous distribution of nanoscale （10-20 nm） metal particles in the oligomer matrix. A computational model of the group and elemental composition of nanometallocarbosilanes was developed; it was shown that they are molecular globules of near-spherical shape and rigid polycyclic structure. Thermochemical treatment of nanometallocarbosilanes leads to SiC-nanoceramics （a high yield of up to 75-80 mass%） modified by metal nanoparticles （20-30 nm） contributing to its stabilization. The application of preceramic oxygen-free nanometallocarbosilanes will make it possible to advance in solving the problem of ceramic composite materials with long-term resistance at temperatures above 1,500 ℃ in oxidizing environments.

关键词： Nanometallocarbosilanes zirconium hafnium tantalum alkyl amides.

俄罗斯北堪察加Evevpenta金矿床玄武岩矿物次生蚀变特征及其成矿指示作用

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地学前缘 2023年第5期30卷 450-468页

作者： Anastasiya SERGEEVA Pavel ZHEGUNOV Elena SKILSKAIA mariya NAZAROVA Elena KARTASHEVA Anna KUZMINA Svetlana MOSKALEVA Olesya ZOBENKO Sharapat KUDAEVA Ekaterina PLUTAKHINA Kseniya SHISHKANOVA Institute of Volcanology and Seismology Far Eastern BranchRussian Academy of SciencesPiip Boulevard9Petropavlovsk-Kamchatsky 683006Russia

俄罗斯北堪察加地区Evevpenta金矿床玄武岩主岩青磐岩化蚀变的矿物学组成,是以英安岩中的石英、冰长石-石英、石英-碳酸岩岩脉和热液角砾岩组成的脉系为特征。脉状矿物是天然金、碲化物、硫化物、硒化物和金-银氯化物。青磐岩化蚀变发... 详细信息

俄罗斯北堪察加地区Evevpenta金矿床玄武岩主岩青磐岩化蚀变的矿物学组成,是以英安岩中的石英、冰长石-石英、石英-碳酸岩岩脉和热液角砾岩组成的脉系为特征。脉状矿物是天然金、碲化物、硫化物、硒化物和金-银氯化物。青磐岩化蚀变发生在距浅成热液脉较远的地方。然而,它的特征矿物组合对重建成矿环境有重要研究意义。在青磐岩化带内可识别出氟磷灰石-氟铝钛矿、石英-方解石-斜绿泥石、石英-方解石-硬石-蒙脱石、方解石-丝光沸石4种不同的热液蚀变组合。最早的矿物组合形成于酸性富F的热液中。热液流体中氟化物浓度为0.2~1.2 g/L,流体温度约550℃。酸性热液与橄榄玄武岩寄主岩石长期相互作用,导致pH升高至接近中性并发生绿泥石化作用。绿泥石化阶段热液富集Fe和Mg,温度约175~210℃时形成第1阶段绿泥石,120~140℃时形成第2阶段绿泥石。蚀变的最后阶段形成沸石群矿物,其在pH约为9、温度>135℃的热液中析出。热液流体的高pH值可能是由于碳酸氢盐和碳酸盐的存在以及溶液中铁盐的去除。根据青磐岩化带蚀变矿物组合特征,探讨了Evevpenta脉系形成的物理化学条件。在热液体系形成阶段,随着碱性氯化钠溶液的循环,贵金属Cl~-和OH~-络合物的形成导致了矿石元素的运移。高pH溶液也能转移硫。

关键词：矿物学金丝光沸石斜绿泥石青磐岩化蚀变 Evevpenta 堪察加

Parents’s knowledge and awareness about hepatitis B can influence the vaccination of their children

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World Journal of Virology 2024年第2期13卷 96-106页

作者： Nanda Chhavi Geetika Srivastava mariya Waseem Abhishek Yadav Surender Singh Rajani Singh Amit Goel Department of Paediatrics Era’s Lucknow Medical CollegeLucknow 226003Uttar PradeshIndia Medical Consultant National Disease Control ProgramNational Health MissionLucknow 226003Uttar PradeshIndia Department of Hepatology Sanjay Gandhi Postgraduate Institute of Medical SciencesLucknow 226014Uttar PradeshIndia

BACKGROUND Birth-dose(Hep-BD)followed by three additional doses(Hep-B3)of hepatitis B virus(HBV)vaccine are key to eliminating HBV by ***,Hep-BD and Hep-B3 coverage in our country is *** To studied the parent’s knowledge and awareness about HBV infection,its prevention,consequences and *** Parents of 6 months to 8 years old children were interviewed to assess their knowledge&awareness about hepatitis B,its transmission,prevention,illness caused by this,and *** close-ended questions were admini-stered,and responses were recorded as‘yes’,‘no’,or‘not sure’.HBV knowledge score was calculated based on the sum of correct *** correct response scored one point and incorrect,missing or‘not sure’responses received no *** data are presented as number(%)and numerical data are expressed as *** were compared using Chi2 tests and level of significance was kept as P<*** Parents(58.3%mothers)of 384 children(89.9%age<5 years;82%age-appropriately vaccinated)were *** hundred and twenty-two(83.9%)children were Hep-B3 vaccinated.94.3%,87.5%,and 29.2%parents knew about polio,tetanus,and hepatitis B ***,41.2%,15.8%,and 23%parents knew about hepatitis B transmission,consequences of infection,and prevention *** 7.6%parents knew about three-dose schedule of hepatitis B *** 23%parents believed that vaccine could prevent HBV,15.7%knew that HBV affects *** of Hep-B3 vaccinated children were significantly more aware about HBV than the parents of unvaccinated children(P<0.05 for 17/18 questions).CONCLUSION The knowledge and awareness among the parents about hepatitis B is *** Increasing knowledge/awareness about HBV among parents may improve Hep-B3 vaccination coverage.

关键词： Hepatitis B Viral hepatitis Cirrhosis Hepatocellular carcinoma Hepatotropic viruses Transfusion transmitted infection Mother to child transmission

Fetal stem cells are effective in the treatment of Grade Ⅰ and Ⅱrespiratory failure in amyotrophic lateral sclerosis and muscular dystrophy

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Translational Neuroscience and Clinics 2015年第1期1卷 10-16页

作者： Nataliia.S..Sych Olena.V..Ivankova mariya.O..Klunnyk Iryna.G..Matiyashchuk Andrey.A..Sinelnyk mariya.P..Demchyk Maryna.V..Skalozyb Dario.Siniscalco Department of Clinical Cell Therapy Center Em Cell Department of Skalozyb‐Biotechnology Cell Therapy Center Em Cell Department of Experimental Medicine Second University of Naples via S.Maria di Costantinopoli 16

Objectives: To study the effect of fetal stem cell(FSC) therapy on Grade Ⅰ and Ⅱrespiratory failure in patients with amyotrophic lateral sclerosis(ALS) and muscular dystrophy(MD). Methods: A comparative study was conducted on 41 patients with Grade Ⅰ or Ⅱrespiratory failure(RF) resulting from ALS or MD. The patients were divided into 4 groups according to the underlying disease and the degree of RF. Patients underwent combined treatment, including the experimental application of FSC therapy, and were examined before FSC treatment, and 6 months and 12 months after treatment. Results: FSC treatment improved both subjective and objective breathing parameters as early as 6 months post‐treatment. A significant increase in the forced vital capacity(FVC) and forced expiratory volume in 1 second(FEV1) was reported by all patients with grade Ⅰ RF linked to ALS and MD compared to baseline. Patient respiratory improvement was maintained over the next 6 months. Grade Ⅱ RF patients with MD reported a significant improvement in FVC 12 months after treatment. Conclusions: Evidence for respiratory improvement was observed as early as 6 months in all patients after combined treatment including FSC therapy, and this was maintained for a further 6 months after therapy. In MD patients with Grade Ⅱ RF, treatment resulted in a significant FVC and FEV1 increase within 6 months and downgrading to Grade Ⅰ RF within a year after FSC treatment.

关键词： respiratory failure amyotrophic lateral sclerosis muscular dystrophy fetal stem cells forced vital capacity forced expiratory volume

Neuroimaging Phenomenology of the Central Self-Regulation Mechanisms

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Journal of Behavioral and Brain Science 2014年第1期4卷 58-68页

作者： Mark B. Shtark Kseniya Mazhirina mariya Rezakova Andrey Savelov Mikhail Pokrovskiy Olga Jafarova Company “Biofeedback Computer Systems” Novosibirsk Russia Research Institute “International Tomography Center” Siberian Branch of the Russian Academy of Sciences Novosibirsk Russia Research Institute of Molecular Biology and Biophysics Siberian Branch of the Russian Academy of Medical Sciences Novosibirsk Russia

Functional MRI was used to map the brains of subjects on-line during the process of media training for the acquisition and improvement of self-regulation mechanisms. The temporal and spatial dynamics of the new neural network formation were studied in real and simulated (false) biofeedback game, and their qualitative characteristics were discussed. It has been shown that immersion into a virtual competitive game, controlled by physiological responses, causes a wide involvement of the cortices, characterized by a high volume of activation in the mid-temporal, occipital and frontal areas, the cuneus and the precuneus. In both forms of media training, high values of activation volume were identified in the cerebellar structures.

关键词： Functional Magnetic Resonance Imaging Adaptive Feedback Real and Imitation Biofeedback Self-Regulation Strategies