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International Journal of Sediment Research 2023年第3期38卷 303-315页

作者： mario Misale Johan Augusto Bocanegra Annalisa Marchitto DIME-Department of Mechanics Energetics Management and Transportation Thermal Engineering and Environmental Conditioning Division University of Genoa

The stability of nanofluids is critical in engineering applications. The sedimentation of the nanoparticles in the base fluid limits the stability of the nanofluid. By measuring the absorbance of a visible laser through water aluminum oxide nanofluids it is possible to observe the evolution of the sedimentation process. A simple experimental setup consisting of a thin closed test cavity（0.8 mm or 1.5 mm thickness）filled with nanofluid and a set of laser diodes-photodiodes pairs was used in the experiments to determine the absorbance and the local volumetric concentration. The evolution of the local volumetric concentration of the nanofluid was measured at 13 height positions along the test cavity. Five initial volumetric concentrations of aluminum oxide（Al2O3; 0.5%, 1.0%, 1.5%, 2.0%, 3.0% and 4.0%） were considered. Data and digital photos were acquired for a total time lapse of 260 days. The results were independent of the container thickness. The same general trend was consistent for all the initial volumetric concentrations, i.e., the sedimentation rate decreases in time. The time evolution of the volumetric concentration was compared with results from a modified Mason-Weaver model. The comparison was possible by setting a variable sedimentation velocity in the model, suggesting that the sedimentation is affected by the dynamics of nanoparticle clusters with diverse sizes produced by agglomeration.

关键词： Pure nanofluid Aluminum oxide Nanoscale alumina particles Colloid sedimentation Nanoparticle settling Mason-Weaver model

Preparation and Structural Characterization of Rapidly Solidified Al-Cu Alloys

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Journal of Materials Science & Technology 2014年第4期30卷 394-400页

作者： Iuliana Lichioiu Ildiko Peter Bela Varga mario Rosso Department of Materials Science Transilvania University of Brasov Department of Applied Science and Technology Institute of Science & Engineering of Materials for the Innovative Technologies

Rapidly solidified Al100-x-Cux alloys （x = 5, 10, 15, 25, 35 wt%） were prepared and analyzed. High cooling rate increased the Cu solubility in α-AI matrix. The influence of the cooling rate on Cu solubility extension in AI was experimentally simulated. Thus the pouring was performed in metallic die and by melt spinning-low pressure （MS- LP） technique. Melt processing by liquid quenching was performed using a self-designed melt spinning set-up which combined the cooling technology of a melt jet on the spinning disc with the principle of the mold feeding from low pressure casting technology. The thickness of the melt-spun ribbons was in the range of 30-70 μm. The cooling rate provided by MS-LP was within 105-106 K/s after the device calibration. The obtained alloys were characterized from structural, thermal and mechanical point of view. Optical microscopy and scanning electron microscopy were employed for the microstructural characterization which was followed by X-ray analysis. The thermal properties were evaluated by dilatometric and differential scanning calorimetric measurements. Vickers microhardness measurements were performed in the study. In the case of the hypereutectic alloy with 35 wt% Cu obtained by MS-LP method, the microhardness value increased by 45% compared to the same alloy obtained by gravity casting method. This was due to the extended solubility of the alloying element in the α-AI solid solution.

关键词： AI-Cu alloy Melt spinning Fractographs Extended solubility

Helicobacter heilmannii sensu lato :An overview of the infection in humans

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World Journal of Gastroenterology 2014年第47期20卷 17779-17787页

作者： mario Bento-Miranda Ceu Figueiredo Department of Pathology and Oncology Faculty of Medicine of the University of Porto4200-465 PortoPortugal Institute of Molecular Pathology and Immunology of the University of Porto 4200-465 PortoPortugal

Helicobacter heilmannii sensu lato(*** s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each *** *** the stomach of several animals and may have zoonotic *** the prevalence of these infections in humans is low,they are associated with gastric pathology,including mucosa-associated lymphoid tissue lymphoma,making them a significant health ***,the taxonomy,epidemiology,microbiology,diagnosis,and treatment of these infections will be *** gastric pathology associated with *** *** in humans will also be ***,the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be *** understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute *** *** essential for developing strategies for prevention and treatment of these infections.

关键词： Helicobacter heilmannii sensu lato Gastric non-Helicobacter pylori Helicobacter species Pathogenesis Diagnosis Treatment Genomes

Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第4期13卷 244-247页

作者： Bogdan MUNTEANU mario BRAUN Kajohn BOONROD RLP AgroScience GmbH AlPlanta-Institute for Plant ResearchBreitenweg 71D-67435 NeustadtGermany

QuickChange mutagenesis is the method of choice for site-directed mutagenesis （SDM） of target sequences in a plasmid. It can be applied successfully to small plasmids （up to 10 kb）. However, this method cannot efficiently mutate bigger plasmids. Using KOD Hot Start polymerase in combination with high performance liquid chromatography （HPLC） purified primers, we were able to achieve SDM in big plasmids （up to 16 kb） involving not only a single base change but also multiple base changes. Moreover, only six polymerase chain reaction （PCR） cycles and 0.5μI of polymerase （instead of 18 PCR cycles and 1.0 μI of enzyme in the standard protocol） were sufficient for the reaction.

关键词： Site-directed mutagenesis (SDM) Mutant Plasmid

Synthetic polycistronic sequences in eukaryotes

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Synthetic and Systems Biotechnology 2021年第4期6卷 254-261页

作者： Xuekun Wang mario Andrea Marchisio School of Pharmaceutical Science and Technology Tianjin University92 Weijin Road300072TianjinChina

The need for co-ordinate,high-level,and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic *** work outlines the functionality and design of IRES-and 2 A-peptide-based constructs by comparing different strategies for co-expression in polycistronic *** particular,2 A sequences are small peptides,mostly derived from viral polyproteins,that mediate a ribosome-skipping event such that several,different,separate proteins can be generated from a single open reading *** applied to metabolic engineering and synthetic gene circuits,2 A peptides permit to achieve co-regulated and reliable expression of various genes in eukaryotic cells.

关键词： Synthetic biology 2A peptide IRES Polycistronic sequence

Driver Intent Prediction and Collision Avoidance With Barrier Functions

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IEEE/CAA Journal of Automatica Sinica 2023年第2期10卷 365-375页

作者： Yousaf Rahman Abhishek Sharma Mrdjan Jankovic mario Santillo Michael Hafner the Ford Motor Company DearbornMichigan 48121 USA IEEE

For autonomous vehicles and driver assist systems,path planning and collision avoidance algorithms benefit from accurate predictions of future location of other vehicles and intent of their *** the literature,the algorithms that provide driver intent belong to two categories:those that use physics based models with some type of filtering,and machine learning based *** this paper we employ barrier functions(BF)to decide driver *** are typically used to prove safety by establishing forward invariance of an admissible ***,we decide if the“target”vehicle is violating one or more possibly fictitious(i.e.,non-physical)barrier constraints determined based on the context provided by the road *** algorithm has a very small computational footprint and better false positive and negative rates than some of the *** predicted intent is then used by a control barrier function(CBF)based collision avoidance system to prevent unnecessary interventions,for either an autonomous or human-driven vehicle.

关键词： Driver Intent Prediction and Collision Avoidance With Barrier Functions Intent

Free charge localization and effective dielectric permittivity in oxides

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Journal of Advanced Dielectrics 2016年第2期6卷 39-51页

作者： mario Maglione ICMCB-CNRS University of Bordeaux 87Av Dr Schweitzer 33608 PessacFrance

This review will deal with several types of free charge localization in oxides and their consequences on the effective dielectric spectra of such *** first one is the polaronic localization at the unit cell scale on residual impurities in ferroelectric *** second one is the collective localization of free charge at macroscopic interfaces like surfaces,electrodes and grain boundaries in *** have been observed in many oxide perovskites mostly when cations having several stable electronic configurations are *** manganites,the density of such polarons is so high as to drive a net lattice of interacting *** the other hand,in ferroelectric materials like BaTiO_(3)and LiNbO_(3),the density of polarons is usually very small but they can influence strongly the macroscopic *** contribution of such polarons to the dielectric spectra of ferroelectric materials is *** residual impurities as for example Iron can induce well-defined anomalies at very low *** is mostly resulting from the interaction between localized polarons and the highly polarizable ferroelectric network in which they are *** case of such residual polarons in SrTiO_(3)will be described in more detail,emphasizing the quantum polaron state at liquid helium ***,several nonferroelectric oxides have been shown to display giant effective dielectric *** is first shown that the frequency/temperature behavior of such parameters is very similar in very different compounds(donor-doped BaTiO_(3),CaCu_(3)Ti_(4)O_(12),LuFe_(2)O_(4),Li-doped NiO,etc.).This similarity calls for a common origin of the giant dielectric permittivity in these compounds.A space charge localization at macroscopic interfaces can be the key for such extremely high dielectric permittivity.

关键词： Charged point defects ferroelectric oxides Perovskites space charges polarons

Mysticism and Peace of Mind： Reflections on Tugendhat and Daoism

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Frontiers of Philosophy in China 2017年第4期12卷 554-571页

作者： mario Wenning Philosophy and Religious Studies Programme University of Macao Macao China

Philosophical mysticism is irrational union with a transcendent reality often understood as involving an This paper challenges this assumption by examining the universal and rational potentials of mysticism. Drawing on Ernst Tugendhat＇s interpretation of mysticism as an overcoming of egocentricity and a pursuit of peace of mind, it focuses on philosophical Daoism as a distinctive form of mysticism that emphasizes the rationality of stepping back from one＇s excessive volitional attachments in light of the validity of other perspectives. Mysticism, thus conceived, has a distinctive potential that does not depend on religious revelation or ineffable experience. Its genuine appeal consists in stepping back from oneself and one＇s desires, even including the pursuit of peace of mind.

关键词： mysticism perspectivism peace of mind Daoism Tugendhat

The Hsp90-binding immunophilin FKBP52 enhances neurodifferentiation and neuroregeneration in murine models

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Neural Regeneration Research 2022年第3期17卷 555-556页

作者： Cristina Daneri-Becerra mario D.Galigniana Instituto de Biología y Medicina Experimental/CONICET&Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos AiresArgentina

The term immunophilin involves a family of proteins whose domain shows peptidylprolyl-(cis/trans)-isomerase (PPIase)enzymatic activity,i.e., the reversible cis/trans interconversion of Xaa-Pro bonds (Annett et al.,2020).The PPlase domain of these proteins usually binds to immunosuppressive drugs,such as the macrolide FK506 (referred to as the FKBP subfamily) or the cyclic undecapeptide cyclosporine A (called CyP subfamily).

关键词： bonds reversible enzymatic

Tragic Recognition:Revisiting Hegel's Conception of Ethical Life

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Frontiers of Philosophy in China 2018年第4期13卷 483-504页

作者： mario Wenning Philosophy and Religious Studies Programme University ofMacaoMacaoChina

This paper interprets Hegel's engagement with tragedy and especially tragic action as an interpretive model for understanding ethical life in complex societies in which independent value spheres collide. Tragic recognition, in contrast to the kind of recognition introduced in the master and slave dialectic, is not based on desire, but arises from the suffering deriving from clashing value spheres. As a coming to terms with one's finitude, tragic recognition presents an important corrective to the account of mutual recognition that has been the reference point of contemporary interpretations of Hegel's social and political philosophy. The paper concludes by pointing to some of the limits of tragedy as a universal interpretive framework for modern societies.

关键词： Hegel tragedy recognition ethical life death