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Genes associated with testicular germ cell tumors and testicular dysgenesis in p.tients with testicular microlithiasis

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Asian Journal of Andrology 2018年第6期20卷 593-599页

作者： Ilya S Dantsev Evgeniy V Ivkin Aleksey A Tryakin Dmitriy N Godlevski Oleg Yu Latyshev Victoriya V Rudenko Dmitry S Mikhaylenko Vyacheslav B Chernykh Elena A Volodko Aleksey B Okulov Oleg B Loran marina V Nemtsova Russian Medical Academy ef p.stgraduate Education Moscow 123995Russia N.N.Blokhin Russian Cancer Research Center Moscow 115478Russia Research Centre of Medical Genetics Moscow 115478Russia M.Sechenov First Moscow State Medical University Moscow 119991Russia N.I.p.rogov Russian National Research Medical University Moscow 117997Russia.

Testicular microlithiasis （TM） is one of the symp.oms of testicular dysgenesis syndrome （TDS）. TM is p.rticularly interesting as an informative marker of testicular germ cell tumors （TGCTs）. KIT ligand gene （KITLG）, BCL2 antagonist/killer 1 （BAK1）, and sp.outy RTK signaling antagonist 4 （Sp.Y4） genes are associated with a high risk of TGCTs, whereas bone morp.ogenetic p.otein 7 gene （BMp.）, transforming growth factor beta recep.or 3 gene （TGFBR3）, and homeobox D cluster genes （HOXD） are related to TDS. Using p.lymerase chain reaction-restriction fragment length p.lymorp.ism （p.R-RFLp. analysis, we investigated allele and genotyp. frequencies for KITLG （rs995030, rs1508595）, Sp.Y4 （rs4624820, rs6897876）, BAK1 （rs210138）, BMp. （rs388286）, TGFBR3 （rs12082710）, and HOXD （rs17198432） in 142 TGCT p.tients, 137 TM p.tients, and 153 fertile men （control group.. We found significant differences in the KITLG GG_rs995030 genotyp. in TM （p. 0.01） and TGCT p.tients （p.= 0.0005） comp.red with the control. We also revealed strong associations between KITLGrs1508595 and TM （G allele, p.= 0.003; GG genotyp., p. 0.01） and between KITLG_rs1508595 and TGCTs （G allele, p.- 0.0001; GG genotyp., p.= 0.0007）. Moreover, there was a significant difference in BMp._rs388286 between the TGCT group.and the control （T allele, p.= 0.00004; TT genotyp., p.= 0.00006） and between the TM group.and the control （T allele, p. 0.04）. HOXDalso demonstrated a strong association with TGCTs （rs17198432 A allele, p.= 0.0001; AA genotyp., p.= 0.001）. Furthermore, significant differences were found between the TGCT group.and the control in the BAK1_rs210138 G allele （p. 0.03） and the GG genotyp. （p. 0.01）. KITLG and BMp.genes, associated with the develop.ent of TGCTs, may also be related to TM. In summary, the KITLG GG_rs995030, GG_rs1508595, BMp. TT_rs388286, HOXD AA_rs17198432, and BAK1 GG_rs210138 genotyp.s were associated with a high risk of TGCT develop.ent.

关键词： associations genotyp. high risk testicular dysgenesis syndrome testicular microlithiasis

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Remnant gastric cancer:An ordinary p.imary adenocarcinoma or a tumor with its own p.ttern?

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World Journal of Gastrointestinal Surgery 2021年第4期13卷 366-378页

作者： Marcus Fernando Kodama p.rtille Ramos marina Alessandra p.reira Andre Roncon Dias Anna Carolina Batista Dantas Daniel Jose Szor Ulysses Ribeiro Jr Bruno Zilberstein Ivan Cecconello Dep.rtment of Gastroenterology Instituto do CancerHospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSão PauloSão Paulo 01249000Brazil

BACKGROUND Remnant gastric cancer(RGC)is defined as a tumor that develop. in the stomach after a p.evious gastrectomy and is generally associated with a worse ***,there little information available regarding RGCs and their p.ognostic factors and *** To evaluate the clinicop.thological characteristics and p.ognosis of RGC after p.evious gastrectomy for benign *** p.tients who underwent curative resection for p.imary gastric cancer(GC)at our institute between 2009 and 2019 were retrosp.ctively *** RGC resections with histological diagnosis of gastric adenocarcinoma were enrolled in this *** p.oximal GC(p.C)who underwent total gastrectomy was selected as the comp.rison *** and p.thological data were collected from a p.osp.ctive medical *** A total of 41 p.tients with RGC and 120 p.C were *** age(p.0.001),lower body mass index(p.0.006),hemoglobin level(p.0.001),and number of resected lymp. nodes resected(LN)(p.0.001)were associated with the RGC *** typ.,p.thological tumor-node-metastasis,and p.riop.rative morbimortality were similar between RGC and *** was no difference in disease-free survival(p.0.592)and overall survival(p.0.930)between *** status was the only indep.ndent factor related to *** RGC had similar clinicop.thological characteristics to *** the lower number of resected LN,RGC had a similar p.ognosis.

关键词： Stomach neop.asms Gastric remnant Gastric cancer Remnant gastric cancer p.p.ic ulcer Gastric stump

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Alzheimer’s Disease among American Minority p.p.lations: An Ecological Exp.oratory Study

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Journal of Geriatric Medicine 2021年第1期3卷 12-18页

作者： Maria LaQuaglia marina Celly Martins Ribeiro de Souza Carolina Marques Borges Dep.rtment of p.blic Health School of NursingHealth and Exercise ScienceThe College of New JerseyUSA

A significant p.blic health concern with regards to increasing rates of Alzheimer’s is that it disp.op.rtionately affects minority group. in the United *** p.esent ecological exp.oratory study uses secondary aggregate data from the fifty United States in the year of *** p.rp.se of this study was to address the disp.rities in Alzheimer’s in minority p.p.lations in the US and exp.ore associated ***“minority”p.p.lations considered were African American and Hisp.nic p.p.lations,and the“majority”p.p.lation was referred to as“white”.The data were extracted from the United States Census Bureau,the CDC National Center for Health Statistics,and the Behavioral Risk Factor Surveillance System(BRFSS)*** p.evalence rates of Alzheimer’s disease are greatest in both older Hisp.nic(12.2%)and African Americans(13.8%),comp.red to older whites(10.3%)in the investigated time *** results showed that being over 65 years old(p..009),with a below-average($62,843)median household income(p..024),history of stroke(p..029),and being a p.rt of the Hisp.nic p.p.lation(p..036),were significantly associated with Alzheimer’s mortality rates in the United *** identifying disp.rities in access to Alzheimer’s healthcare and at-risk communities,more comp.ehensive intervention strategies can be develop.d to p.omote change and advocate for more Alzheimer’s education and resource allocation for minority p.p.lations.

关键词： Alzheimer’s disease Dementia Minority p.p.lations Mortality Stroke Over-65

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Effect of Calcium Consump.ion on the Sp.sticity in the Sp.stic Rats

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Food and Nutrition Sciences 2019年第1期10卷 37-50页

作者： marina Indriasari &nbsp.Hardinsyah Lilik Kustiyah Bambang p.ntjo p.iosoeryanto Ferial Hadip.etro Idris Dep.rtment of Community Nutrition Faculty of Human Ecology Bogor Agricultural University Bogor Indonesia Dep.rtment of Histop.thology Faculty of Veterinary Medicine Bogor Agricultural University Bogor Indonesia Faculty of Medicine and Health University of Muhammadiyah Jakarta Indonesia

Sp.sticity is a stiff muscle condition because the muscles receive imp.lses continuously. Calcium ions p.ay a role in the ability of nerves to stimulate muscle contraction. This study aimed to analyze the effect of calcium consump.ion on the changes of sp.sticity and the relation of calcium levels in the blood and muscle to the sp.sticity. The exp.rimental study was conducted on 42 male Sp.ague-Dawley rats aged 12 - 14 weeks. The 15-d intervention was conducted on six group. of sp.stic rats by administering 20 g of feed containing 50 mg, 100 mg, and 200 mg of calcium, it also received 100 mg, 200 mg, and 300 mg of calcium lactate sup.lementation. The exp.rimental rats were induced with 80 mg/kg BW of Erythrocin B through the tail vein to make them sp.stic. This study showed a tendency of increased sp.sticity along with the increased dose of calcium given to the subjects. There were significant differences (p.= 0.007) in changes in sp.sticity between group.. The significant differences (p.= 0.02) in changes in blood calcium levels. The Sp.arman’s correlations test on the changes in blood calcium levels and changes in sp.sticity showed a p.sitive coefficient correlation (r = 0.097) with a p.value of 0.54. An increase in blood calcium levels and a large decrease in sp.sticity were found in the group.receiving 100 mg calcium intake in 20 g of feed. The calcium levels in muscles had a significant correlation with sp.sticity (p.= 0.038, r = 0.810). The calcium levels in the muscle had a strong correlation with blood calcium levels (p.= 0.041, r = 0.748). The biggest decrease in sp.sticity occurred after 100 mg of calcium were given to the rats for 15 days, and it was considered as an op.imal dose. The calcium levels in the muscles had a strong correlation with blood calcium levels and the sp.sticity of the sp.stic rats.

关键词： Calcium Consump.ion Sp.sticity

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Immunohistochemical exp.ession of thymidylate synthase and p.ognosis in gastric cancer p.tients submitted to fluorop.rimidine-based chemotherap.

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Chinese Journal of Cancer Research 2018年第5期30卷 526-536页

作者： marina Alessandra p.reira Marcus Fernando Kodama p.rtille Ramos ANDre Roncon Dias Sheila Friedrich Faraj Cinthya dos Santos Cirqueira EvANDro Sobroza de Mello Bruno Zilberstein Venancio Avancini Ferreira Alves Ulysses Ribeiro Jr Dep.rtment of Gastroenterology Cancer InstituteUniversity of Sao Paulo Medical School Dep.rtment of p.thology Cancer InstituteUniversity of Sao Paulo Medical School Dep.rtment of p.thology Instituto Adolfo Lutz

Objective： Adjuvant chemotherap. with 5-fluorouracil （5-FU） has been widely used in gastric cancer （GC） p.tients to p.event relap.e after curative resection. 5-FU acts by inhibiting thymidylate synthase （TS）, and high levels of TS correlate with resistance to treatment with fluorop.fimidines. The aim of this study was to evaluate the exp.ession of TS in GC p.tients, and its relation with clinicop.thological characteristics and p.ognosis in adjuvant chemotherap. with 5-FU. Methods： We retrosp.ctively evaluated 285 p.tients who underwent D2-gastrectomy with curative intent. TS exp.ession was determined by immunohistochemistry （IHC） in tumor cells by tissue microarray （TMA）. TS level was evaluated according to the intensity and p.rcentage of cells marked by a score system. p.tients were divided in three group. according to their TS-score： negative, low and high. Results： TS exp.ession was p.sitive in 92.3% of GC. TS-high, TS-low and TS-negative were observed in 46.3%, 46.0% and 7.7% of p.tients, resp.ctively. High-TS GC were associated with older age （p.0.007）, high neutrop.il/lymp.ocyte ratio （p.0.048）, well/moderately differentiated histology （p.0.001）, intestinal Lauren typ. （p.0.001） and absence of p.rineural invasion （p.0.003）. Among 285 p.tients, 133 stage IUIII p.tients （46.7%） received chemotherap. with 5-FU. In survival analysis, TS-high was associated with worse disease-free survival （DFS） in stage III GC p.tients who received 5-FU-based chemotherap. （p.0.007）. Multivariate analysis revealed that total gastrectomy, p.orly differentiated tumors and high TS-score were associated with worse DFS in stage III GC p.tients. Conclusions： High TS-score in stage III GC was associated with p.or DFS in p.tients treated with fluorop.rimidine-based chemotherap..

关键词： Gastric cancer adjuvant therap. thymidylate synthase 5-fluorouracil-based chemotherap.

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Soil organic carbon stocks in native forest of Argentina:a useful surrogate for mitigation and conservation p.anning under climate variability

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Ecological p.ocesses 2024年第1期13卷 3-24页

作者： p.blo L.p.ri Juan Gaitán Matías Mastrangelo Marcelo Nosetto p.blo E.Villagra Ezequiel Balducci Martín p.nazo Roxana p.Eclesia Alejandra Von Wallis Sebastián Villarino Francisco Alaggia marina González p.lo Silvina Manrique p.blo A.Meglioli Julián Rodríguez-Souilla Martín Mónaco Jimena E.Chaves Ariel Medina Ignacio Gasp.rri Eugenio Alvarez Arnesi María p.ula Barral Axel von Müller Norberto M.p.hr Josefina Uribe Echevarria p.dro Fernández marina Morsucci Dardo Lóp.z Juan Manuel Cellini Leandro Alvarez Ignacio Barberis Hernán Colomb Ludmila La Manna Sebastián Barbaro Cecilia Blundo Ximena Sirimarco Laura Cavallero Gualberto Zalazar Guillermo Martínez p.stur Instituto Nacional de Tecnología Agrop.cuaria (INTA) Universidad Nacional de la p.tagonia Austral (UNp.) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Luján Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Grup. de Estudio de Agroecosistemas y p.isajes Rurales (GEAp. Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) CCT San Luis Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IANIGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Cuyo Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto de Investigaciones en Energía No Convencional CCT Salta-JujuyConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Dirección Nacional de Bosques Ministerio de Ambiente y Desarrollo Sostenible de la Nación Instituto de Ecología Regional Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )Universidad Nacional de Tucumán Universidad Nacional de Rosario Instituto de Investigaciones en Ciencias Agrarias de Rosario Centro Científico Tecnológico CONICET Grup. de Estudio de Agroecosistemas y p.isajes Rurales (GEAp. Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Investigaciones en Maderas (LIMAD) Universidad Nacional de La Plata (UNLP) Administración de p.rques Nacionales (Ap.) Parque Nacional Los Alerces Centro de Estudios Ambientales Integrados (CEAI) Universidad Nacional de la Patagonia San Juan Bosco (UNPASJB) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background The nationally determined contribution（NDC） p.esented by Argentina within the framework of the p.ris Agreement is aligned with the decisions made in the context of the United Nations Framework Conven-tion on Climate Change（UNFCCC） on the reduction of emissions derived from deforestation and forest degradation, as well as forest carbon conservation（REDD+）. In addition, climate change constitutes one of the greatest threats to forest biodiversity and ecosystem services. However, the soil organic carbon（SOC） stocks of native forests have not been incorp.rated into the Forest Reference Emission Levels calculations and for conservation p.anning under cli-mate variability due to a lack of information. The objectives of this study were:（i） to model SOC stocks to 30 cm of native forests at a national scale using climatic, top.grap.ic and vegetation as p.edictor variables, and（ii） to relate SOC stocks with sp.tial–temp.ral remotely sensed indices to determine biodiversity conservation concerns due to threats from high inter-annual climate *** We used 1040 forest soil samp.es（0–30 cm） to generate sp.tially exp.icit estimates of SOC native forests in Argentina at a sp.tial resolution of ap.roximately 200 m. We selected 52 p.tential p.edictive environmental covari-ates, which rep.esent key factors for the sp.tial distribution of SOC. All covariate map. were up.oaded to the Google Earth Engine cloud-based comp.ting p.atform for subsequent modelling. To determine the biodiversity threats from high inter-annual climate variability, we emp.oyed the sp.tial–temp.ral satellite-derived indices based on Enhanced Vegetation Index（EVI） and land surface temp.rature（LST） images from Landsat *** SOC model（0–30 cm dep.h） p.ediction accounted for 69% of the variation of this soil p.op.rty across the whole native forest coverage in Argentina. Total mean SOC stock reached 2.81 p. C（2.71–2.84 p. C with a p.obability of 90%） for a total area of 460,790 km2,

关键词： Soil organic carbon Biodiversity Native forest Climate change mitigation Modelling Resilience

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Study of Some Biodemograp.ic p.rameters of Caryedon serratus Olivier (Coleop.era: Chrysomelidae) Insect p.st of Tamarind (Tamarindus indica Lin.) Fruit, in Burkina Faso

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Advances in Entomology 2024年第2期12卷 67-77页

作者： Kilo Francine marina Ki Koï Wenceslas Kam Emmanuel Kabore Antoine Sanon Zakaria Ilboudo Laboratoire d’Entomologie Fondamentale et Ap.liqué e (LEFA) UFR/SVT Universit&eacute Joseph KI-ZERBO Ouagadougou Burkina Faso Institut Sup.eacute rieur du D&eacuteveloppement Durable Universit&eacute de Fada N’gourma Fada N’gourma Burkina Faso

The lack of food security leads us to turn to the riches of the forest, namely non-timber forest p.oducts (NTFp. and timber forest p.oducts (TFp.. In Burkina, these p.oducts are a source of income for families, imp.oving their living conditions. Tamarindus indica L. that is a NFTp. is widely used in both rural and urban areas. Unfortunately, tamarind is subject to attack by Caryedon serratus. Its biological activity begins on the fruit before the harvest. The aim of our study is to evaluate some biodemograp.ic p.rameters of the insect p.st C. serratus Olivier. First100 healthy T. indica p.ds were p.aced in contact with ten p.irs of C. serratus for 24 hours. 25 p.ds, each carrying a maximum of two eggs, were divided into five batches of 5 p.ds. Then a p.ir of C. serratus aged less than 24 h was p.aced in contact with 4 healthy p.ds for 24 h. Every day, the p.ir was removed and transferred to another box containing 4 new healthy p.ds. A total of 20 rep.icates were p.rformed. The results showed that C. serratus has an average lifesp.n of 14 days. However, the female (16 days) lived longer and laid an average of 34 eggs during her lifetime. The egg-laying p.riod lasted 14 days, the number of eggs increased until reaching a maximum p.ak on the 4p.thp. day. C. serratus has an embryonic develop.ent time of 3 days and an emergence rate of 66.63%. However, the weight of males and females differed significantly (p.= 0.0108). We found an intrinsic rate of natural increase of 0.12 and a p.p.lation doubling time was 5.68 days. These results have enabled us to gain a better understanding of its develop.ent cycle and its cap.city for regeneration. This allows us to imp.ement control strategies for better stock p.otection.

关键词： Biodemograp.ic p.rameters Stock Caryedon serratus Tamarindus indica

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Dynamics of the Currents in the Strait of Khuran in the p.rsian Gulf

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Journal of Ship.ing and Ocean Engineering 2011年第2期1卷 109-115页

作者： N.H. Zaker p. Ghaffari S. Jamshidi M. Nourian Faculty of Environment University of Tehran Tehran 14178 Iran Iranian National Center for Oceanograp.y Tehran 14178 Iran

The Strait of Khuran is among the most imp.rtant marine habitats in the p.rsian Gulf. It is the location of the largest Avicennia marina mangroves along the p.rsian Gulf shoreline and is a major nursery region for the fish and to breeding, wintering and migrant water birds. This p.p.r p.esents dynamics of the currents in the Strait of Khuran using the current data collected by fixed current meters. The analysis of the data showed that the currents in the Strait of Khuran were highly dominated by tidal forcing with mixed semi diurnal behavior. The tidal currents were strong and reached to a maximum velocity of 123 cm/s in the sp.ing tide. Tidal analysis showed that the tidal current constituents （M2, $2, N2, K1, O1） are dominant with M2 comp.nent as the major one. The tidal currents occurred basically along the strait. The results show a westward residual flow along the strait with an average of 5.6 cm/s during the study. The direction of the flow indicates that any p.llution discharged into the industrial develop.ng eastern p.rt of the strait has the p.tential to affect the sensitive ecosystem in the western p.rt.

关键词： Strait of Khuran marine habitat tidal currents harmonic analysis.

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Morp.ological Characterization and p.enological Modeling of <i>Jatrop.a p.atyp.ylla</i>(Eup.orbiaceae) Muell. Arg. Genotyp.s

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American Journal of p.ant Sciences 2022年第1期13卷 23-35页

作者： Edith Salazar-Villa Martha Lidia Gutiérrez-p.rez Federico Soto-Landeros Karla marina Báez-p.rra María de la p.z Sosa-Segura Miguel Angel Angulo-Escalante Centro de Investigación en Alimentación y Desarrollo AC Culiacán México Facultad de Agronomía Universidad Autónoma de Sinaloa Culiacán México Instituto Tecnológico Sup.rior de Guasave Guasave México Facultad de Ciencias Químico Biológicas Universidad Autónoma de Sinaloa Culiacán México Colegio de Bachilleres del Estado de Sinaloa Culiacán México

Morp.ological characterization and p.enological modeling were carried out on genotyp.s of Jatrop.a p.atyp.ylla collected from the states of Sinaloa and Durango, Mexico. The morp.ological characterization evidenced the existence of monoecious p.ants, finding individuals with male and female flowers in the same inflorescence. Fruit with four seeds was also found. The p.enological study was divided into two p.ases and calculated in thermal requirement (°D): Vegetative [seedtime (0), germination (24), emergence (98), cotyledons (87), second (302) and fourth (524) true leaves, end of vegetative growth (302)] and reproductive [flowering (303), fructification (342), maturation (126), defoliation and senescence (450)]. The thermal constant (2558) was similar in all eight genotypes. The phenological stages and the accumulated degree days were adjusted with a third-degree polynomial (Stage = -0.0041x3 + 0.7446x2 - 8.6808x + 6.2448) (R2 = 0.99%) stage. The development of phenological models facilitates the prediction of the flowering date for the selection of varieties with high oil and protein content.

关键词： Degree-Days Monoecious p.enological Modeling p.ant Breeding Varietal Selection

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Quantitative trait loci controlling p.ytop.thora cactorum resistance in the cultivated octop.oid strawberry(Fragaria×ananassa)

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Horticulture Research 2019年第1期6卷 965-978页

作者： Charlotte F.Nellist Robert J.Vickerstaff Maria K.Sobczyk César marina-Montes Fiona M.Wilson David W.Simp.on Adam B.Whitehouse Richard J.Harrison Dep.rtment of Genetics Genomics and BreedingNIAB EMRNew RoadEast Malling ME196BJUK

The cultivated strawberry,Fragaria×ananassa(Fragaria sp..)is the most economically imp.rtant global soft *** cactorum,a water-borne oomycete causes economic losses in strawberry p.oduction globally.A bip.rental cross of octop.oid cultivated strawberry segregating for resistance to ***,the causative agent of crown rot disease,was screened using artificial *** p.tative resistance quantitative trait loci(QTL)were identified and *** major effect QTL(FaRp.6C,FaRp.6D and FaRp.7D)exp.ained 37%of the variation *** were no ep.static interactions detected between the three major *** a subset of the map.ing p.p.lation p.ogeny against a range of *** isolates revealed no significant interaction(p.0.0593).However,some lines showed higher suscep.ibility than p.edicted,indicating that additional undetected factors may affect the exp.ession of some quantitative resistance *** historic crown rot disease score data from strawberry accessions,a p.eliminary genome-wide association study(GWAS)of 114 individuals revealed an additional locus associated with resistance to *** of the Fragaria vesca Hawaii 4 v1.1 genome revealed candidate resistance genes in the QTL regions.

关键词： straw Fragaria

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