检索结果-南通市图书馆

High frequency of CD34+CD38-/low immature leukemia cells is correlated with unfavorable prognosis in acute myeloid leukemia

引用

World Journal of Stem Cells 2017年第12期9卷 227-234页

作者： Adriana Plesa Charles Dumontet Eve Mattei Ines Tagoug Sandrine Hayette pierre Sujobert Isabelle Tigaud marie pierre pages Youcef Chelghoum Fiorenza Baracco Helene Labussierre Sophie Ducastelle Etienne Paubelle Franck Emmanuel Nicolini Mohamed Elhamri Lydia Campos Claudiu Plesa Stéphane Morisset Gilles Salles Yves Bertrand Mauricette Michallet Xavier Thomas Laboratory of Hematology Lyon-Sud HospitalHospices Civils de Lyon CRCL INSERM 1052/CNRS 5286 Department of Hematology Hospices Civils de Lyon Laboratory of Hematology Nord Hospital Statistical and Clinical Research Lyon-Sud HospitalHospices Civils de Lyon Department of Pediatric Hematology and BMT IHOP Lyon

AIM To evaluate the importance of the CD34+CD38-cell population when compared to the CD34+CD38+/low and CD34+CD38+/high leukemic cell sub-populations and to determine its correlations with leukemia characteristics and known prognostic factors, as well as with response to therapy and *** Two hundred bone marrow samples were obtained at diagnosis from 200 consecutive patients with newly diagnosed acute myeloid leukemia(AML) were studied between September 2008 and December 2010 at our Institution(Hematology Department, Lyon, France). The CD34/CD38 cell profile was analyzed by multiparameter flowcytometry approach using 8 C panels and FACS CANTO and Diva software(BD Bioscience).RESULTS We analyzed CD34 and CD38 expression in bone marrow samples of 200 AML patients at diagnosis, and investigated the prognostic value of the most immature CD34+CD38-population. Using a cut-off value of 1% of CD34+CD38-from total "bulk leukemic cells" we found that a high(> 1%) level of CD34+CD38-blasts at diagnosis was correlated with advanced age, adverse cytogenetics as well as with a lower rate of complete response after induction and shorter disease-free survival. In a multivariate analysis considering age, leukocytosis, the % of CD34+ blasts cells and the standardized cytogenetic and molecular risk subgroups, a percentage of CD34+CD38-leukemic cells > 1% was an independent predictor of DFS [HR = 2.8(1.02-7.73), P = 0.04] and OS [HR = 2.65(1.09-6.43), P = 0.03].CONCLUSION Taken together, these results show that a CD34/CD38 "backbone" for leukemic cell analysis by multicolour flowcytometry at diagnosis provides useful prognostic information.

关键词： CD34+CD38-/low Immunophenotyping Leukemic stem cells Acute myeloid leukemia Prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reactivation of latent tuberculosis with TNF inhibitors: critical role of the beta 2 chain of the IL-12 receptor

引用

Cellular & Molecular Immunology 2021年第7期18卷 1644-1651页

作者： marie Robert pierre Miossec Department of Clinical Immunology and Rheumatology and immunogenomics and inflammation research unitUniversity of LyonHôpital Edouard HerriotLyonFrance

Tumor necrosis factor(TNF)inhibitors have improved a lot the treatment of numerous diseases,with the well-known example of rheumatoid arthritis(RA).In the early 2000s,postmarketing data quickly revealed an alarming number of severe tuberculosis(TB)under such *** findings were consistent with previous results in mice where TNF is essential for lymph node formation and granuloma *** effects of TNF inhibition on RA synovium structure are very similar to those on granuloma,with changes in cellular interactions,cytokine,and chemokine *** addition to the role of TNF in granuloma,the interleukin(IL)-12/interferon(IFN)-γpathway is required for an efficient host defense against *** and secondary immunodeficiencies affecting this pathway lead to severe bacillus Calmette-Guérin(BCG)reaction or full *** chronic inflammation as in RA induces a systemic Th1 defect that predisposes to TB through specific downregulation of the IL-12Rß2 *** TNF inhibitors are initiated,this transiently increases this risk of TB,through effects on cellular interactions in a latent TB *** a later stage,when a better control disease activity is obtained,the risk of TB is reduced but not *** the clear benefit from TNF inhibition,latent TB infection screening at baseline is essential for an optimal safety.

关键词： tuberculosis TNF inhibition rheumatoid arthritis cytokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiplexing technology for in vitro diagnosis of pathogens： the key contribution of phosphorus dendrimers

引用

Science China Materials 2018年第11期61卷 1454-1461页

作者： Jean pierre Majoral Jean marie Francois Richard Fabre Alice Senescau Serge Mignani Anne-marie Caminade Laboratoire de Chimie de Coordination du CNRS 31077 Toulouse Cedex 4France LCC-CNRS Universit6 de Toulouse CNRSToulouseFrance Dendris 31670 LabegeFrance LISBP UMR CNRS 5504 INRA 79231077 Toulouse Cedex 04France Universite Paris Descartes PRES Sorbonne Paris Cite CNRS-UMR 860Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicolgique75006 ParisFrance

After the microbiology based on Pasteur's method and polymerase chain reaction(PCR), the diagnosis company named Dendris has proposed a third-generation of diagnosis enabling the search of a broad range of pathogens with strong sensitivity and specificity. This extraordinary profile was possible thanks to the use of phosphorus dendrimers for which various techniques of deposition on a given support were investigated and described and analyzed in this report.

关键词： phosphorus dendrimers diagnosis DNA arrays multiplexing technology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Review on Sources,Extractions and Analysis Methods of a Sustainable Biomaterial:Tannins

引用

Journal of Renewable Materials 2024年第3期12卷 397-425页

作者： Antonio Pizzi marie-pierre Laborie Zeki Candan LERMAB University of LorraineFaculty of SciencesBlvd des AiguillettesNancy54000France Chair of Forest Biomaterials Institute of Earth and Environmental SciencesUniversity of FreiburgFreiburgGermany Freiburg Materials Research Center Alberst Ludwig University of FreiburgFreiburg79104Germany Department of Forest Products Engineering Faculty of ForestryIstanbul UniversitySariyerIstanbul34473Turkey

Condensed and hydrolysable tannins are non-toxic natural polyphenols that are a commercial commodity industrialized for tanning hides to obtain leather and for a growing number of other industrial applications mainly to substitute petroleum-based *** are a definite class of sustainable materials of the forestry *** have been in operation for hundreds of years to manufacture leather and now for a growing number of applications in a variety of other industries,such as wood adhesives,metal coating,pharmaceutical/medical applications and several *** review presents the main sources,either already or potentially commercial of this forestry by-materials,their industrial and laboratory extraction systems,their systems of analysis with their advantages and drawbacks,be these methods so simple to even appear primitive but nonetheless of proven effectiveness,or very modern and *** constitutes a basic but essential summary of what is necessary to know of these sustainable *** doing so,the review highlights some of the main challenges that remain to be addressed to deliver the quality and economics of tannin supply necessary to fulfill the industrial production requirements for some materials-based uses.

关键词： Tannins flavonoids sources extraction methods analysis methods

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In-situ formation of Ni-Al intermetallics-coated graphite/Al composite in a cold-sprayed coating and its high temperature tribological behaviors

引用

Journal of Materials Science & Technology 2017年第6期33卷 507-515页

作者： Chunjie Huang Wenya Li marie-pierre Planche Hanlin Liao Ghislain Montavon ICB UMR 6303 CNRS Univ.Bourgogne Franche-ComtéUTBM State Key Laboratory of Solidification Processing Shaanxi Key Laboratory of Friction Welding Technologies Northwestern Polytechnical University

In this study, cold spraying（CS） was used to deposit a mixture of nickel-coated graphite and 40 vol.% Al powder（Ni-Gr/Al） on a steel substrate aiming to effectively preserve a certain volume fraction of graphite in the deposited Ni-Gr/Al composite coating. The microstructure of the as-sprayed coating and the effect of post-spray heat-treatment（PSHT） temperatures on the in-situ formation of Ni-Al intermetallic phases in coating were studied. The tribological behaviors of the as-sprayed coating and the PSHTed coating under 450？C were tested at 25？C, while the as-sprayed coating was tested at 450？C for *** a result, the Ni-Gr particles showed a homogenous distribution in the coating. The multilayer Ni-Al intermetallics-coated graphite/Al composite coating was achieved in situ after the PSHT of 450？C, where the graphite did decompose at 550？C leaving big pores in the coating. The coefficients of friction（COF）of the CSed coating and the PSHTed coating were measured at 450？C as well as 25？C, which showed a similar tendency, much higher than that of the CSed coating tested at 25？C. The lubrication phase（graphite） improved the formation of a graphite film during sliding friction and decreased the COF, while the hard Ni-Al intermetallic phases contributed to the increase of COF.

关键词： Cold spraying Ni-coated graphite powder Post-spray heat-treatment Intermetallics Tribological performance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

3D models of dilated cardiomyopathy:Shaping the chemical,physical and topographical properties of biomaterials to mimic the cardiac extracellular matrix

引用

Bioactive Materials 2022年第1期7卷 275-291页

作者： marie Camman pierre Joanne Onnik Agbulut Christophe Helary Sorbonne Universit´e CNRSUMR 7574Laboratoire de Chimie de la Matiere Condensee de Paris4 place Jussieu(case 174)F-75005ParisFrance Sorbonne Universite Institut de Biologie Paris-Seine(IBPS)CNRS UMR 8256Inserm ERL U1164Biological Adaptation and Ageing7 quai St-Bernard(case 256)F-75005ParisFrance

The pathophysiology of dilated cardiomyopathy(DCM),one major cause of heart failure,is characterized by the dilation of the heart but remains poorly understood because of the lack of adequate in vitro *** 2D models do not allow for the 3D organotypic organization of cardiomyocytes and do not reproduce the ECM *** this review,the different strategies to mimic the chemical,physical and topographical properties of the cardiac tissue affected by DCM are *** advantages and drawbacks of techniques generating anisotropy required for the cardiomyocytes alignment are *** addition,the different methods creating macroporosity and favoring organotypic organization are ***,the advances in the induced pluripotent stem cells technology to generate cardiac cells from healthy or DCM patients will be *** to the biomaterial design,some features of the DCM extracellular matrix such as stiffness,porosity,topography or chemical changes can impact the cardiomyocytes function in vitro and increase their *** mimicking the affected heart,both at the cellular and at the tissue level,3D models will enable a better understanding of the pathology and favor the discovery of novel therapies.

关键词： Biomaterials Dilated cardiomyopathies Induced pluripotent stem cells Porosity Anisotropy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-magnification observation of seminiferous tubules through the tunica albuginea by two-photon laser scanning microscopy

引用

Asian Journal of Andrology 2011年第5期13卷 774-776页

作者： Vincent Achard Pascal Weber Georges Mercier marie-pierre Blanchard Laboratoire d＇Histologie-Cytologie Faculte de Medecine secteur Timone Universite de la Mediterranee Marseille 13385 France Centre d＇Assistance Medicale a la Procreation AP-HM Conception Marseille 13385 France CNRS - IBDML - UMR6216 Service Imagerie - Case 907 Faculte des Sciences de Luminy Marseille 13288 France and Centre de Microscopie et Imagerie IFR Jean Roche Universite de la Mediterranee Marseille 13916 France

Testicular sperm extraction is widely used in the treatment of male infertility in cases of non-obstructive azoospermia. Identifying spermatogenetic foci within the testes is critical for testicular sperm extraction. Two-photon laser scanning microscopy （TPLSM） is an autofluorescence-based microscopy technique that allows observation at a cellular level in the depth of fresh living tissues and does not require any histological processing （fixation or staining）. The wavelengths previously used have shown no phototoxicity on sperm. We used TPLSM to detect spermatogenetic foci in fresh mouse testicular parenchyma without disrupting the tunica albuginea. Fresh surgically retrieved testes were observed using TPLSM within 1 h after extraction. Contralateral testes for each animal were observed using standard histology. Using TPLSM we were able to observe and measure the diameter of seminiferous tubules through the tunica albuginea, similar to the histological control. Structures within epithelial tubules were also observed, although their nature has yet to be identified. TPLSM is a real-time microscopy technique that could detect spermatogenetic foci.

关键词： seminiferous epithelium spermatogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy

引用

Light(Science & Applications) 2023年第9期12卷 1782-1794页

作者： Clothilde Raoux Anatole Chessel pierre Mahou Gaël Latour marie-Claire Schanne-Klein Laboratory for Optics and Biosciences Ecole PolytechniqueCNRSINSERMInstitut Polytechnique de Paris91128 PalaiseauFrance UniversitéParis-Saclay 91190 Gif-sur-YvetteFrance

A key property of the human cornea is to maintain its curvature and consequently its refraction capability despite daily changes in intraocular *** is closely related to the multiscale structure of the corneal stroma,which consists of 1-3µm-thick stacked lamellae made of thin collagen ***,the distribution,size,and orientation of these lamellae along the depth of the cornea are poorly characterized up to *** this study,we use second harmonic generation(SHG)microscopy to visualize the collagen distribution over the full depth of 10 intact and unstained human corneas(500-600µm thick).We take advantage of the small coherence length in epi-detection to axially resolve the lamellae while maintaining the corneal physiological ***,as raw epi-detected SHG images are spatially homogenous because of the sub-wavelength size of stromal collagen fibrils,we use a polarimetric approach to measure the collagen orientation in every *** a careful validation of this approach,we show that the collagen lamellae(i)are mostly oriented along the inferior-superior axis in the anterior stroma and along the nasal-temporal axis in the posterior stroma,with a gradual shift in between and(ii)exhibit more disorder in the anterior *** results represent the first quantitative characterization of the lamellar structure of the human cornea continuously along its entire thickness with micrometric *** also shows the unique potential of P-SHG microscopy for imaging of collagen distribution in thick dense tissues.

关键词： structure curvature resolved

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tuning Intermolecular Interaction Between Lignin and Carbon Nanotubes in Fiber Composites–A Combined Experimental and Ab-Initio Modeling Study

引用

Journal of Renewable Materials 2018年第3期6卷 325-335页

作者： Jan Badorrek Michael Walter marie-pierre Laborie Freiburger Materialforschungszentrum Stefan-Meier-Straße 21D-79104 Freiburg im BreisgauGermany Chair of Forest Biomaterials Werthmannstraße 6D-79085 Freiburg im BreisgauGermany Freiburg Center for Interactive Materials and Bioinspired Technologies(FIT) Georges-Köhler-Allee 105D-79110 Freiburg im BreisgauGermany Fraunhofer Institute for Mechanics of Materials(IWM) Wöhlerstraße 11D-79108 Freiburg im BreisgauGermany

Doping lignin with carbon nanotubes is a promising strategy for cost-effective high-performance carbon *** investigate the intermolecular interaction potential of CNT and organosolv lignin with two main ***,oxidized purified multiwalled carbon nanotubes(MWCNTs)and beech organosolv lignins and derivatives are analyzed with their Hansen solubility parameters(HSPs)to assess their mutual ***,dispersion-corrected density functional theory simulations of the interaction between model molecules and single-walled carbon nanotubes reveal the source of *** find that oxidation enables and enhances the interaction between carbon nanotubes and organosolv lignin experimentally,which is in agreement with the enhanced polar interaction found in the simulations.

关键词： CNT DFT Hansen solubility parameters lignin oxidation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Substrate Type on Deposition Behavior and Wear Performance of Ni-Coated Graphite/Al Composite Coatings Deposited by Cold Spraying

引用

Journal of Materials Science & Technology 2017年第4期33卷 338-346页

作者： Chunjie Huang Wenya Li Yingchun Xie marie-pierre Planche Hanlin Liao Ghislain Montavon ICB UMR 6303 CNRSUniversite Bourgogne Franche-ComteUTBMF-90100 BelfortFrance State Key Laboratory of Solidification Processing Shaanxi Key Laboratory of Friction Welding TechnologiesNorthwestern Polytechnical UniversityXi'an 710072China

This work focused on the deposition characteristics and wear behavior of Ni-coated graphite mixed with40 vol.% Al（Ni-Gr/Al） composite coatings sprayed on an Al alloy and a steel substrate by cold spraying（CS）. The morphology of the flattened Ni-Gr particles was examined by single-impact tests. Crosssectional microstructure and wear performance of the Ni-Gr/Al composite coatings were studied. Results showed that a larger number of Ni-Gr particles were finally bonded with the steel substrate, whereas many craters existed on the Al alloy substrate after the single-impact tests. The coating on the steel substrate had a high thickness, high graphite content and low coeficient of friction（COF） compared to those on the Al alloy substrate. In addition, the CS coatings presented a homogeneous distribution and uniform morphology of graphite, and a comparative COF to that of conventional thermal sprayed coatings. It was shown that CS could avoid the decomposition and transformation of graphite phase.

关键词： Cold spraying Ni-coated graphite Deposition behavior Microstructure Wear performance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：