检索结果-南通市图书馆

Automated Cell Detection and Morphometry on Growth Plate Images of Mouse Bone

Applied Mathematics 2014年第18期5卷 2866-2880页

作者： maria-grazia ascenzi Xia Du James I. Harding Emily N. Beylerian Brian M. de Silva Ben J. Gross Hannah K. Kastein Weiguang Wang Karen M. Lyons Hayden Schaeffer Department of Orthopaedic Surgery University of California Los Angeles California USA Department of Mathematics University of California Los Angeles California USA Department of Mathematics University of California Irvine California USA

Microscopy imaging of mouse growth plates is extensively used in biology to understand the effect of specific molecules on various stages of normal bone development and on bone disease. Until now, such image analysis has been conducted by manual detection. In fact, when existing automated detection techniques were applied, morphological variations across the growth plate and heterogeneity of image background color, including the faint presence of cells (chondrocytes) located deeper in tissue away from the image’s plane of focus, and lack of cell-specific features, interfered with identification of cells. We propose the first method of automated detection and morphometry applicable to images of cells in the growth plate of long bone. Through ad hoc sequential application of the Retinex method, anisotropic diffusion and thresholding, our new cell detection algorithm (CDA) addresses these challenges on bright-field microscopy images of mouse growth plates. Five parameters, chosen by the user in respect of image characteristics, regulate our CDA. Our results demonstrate effectiveness of the proposed numerical method relative to manual methods. Our CDA confirms previously established results regarding chondrocytes’ number, area, orientation, height and shape of normal growth plates. Our CDA also confirms differences previously found between the genetic mutated mouse Smad1/5CKO and its control mouse on fluorescence images. The CDA aims to aid biomedical research by increasing efficiency and consistency of data collection regarding arrangement and characteristics of chondrocytes. Our results suggest that automated extraction of data from microscopy imaging of growth plates can assist in unlocking information on normal and pathological development, key to the underlying biological mechanisms of bone growth.

关键词： Anisotropic Diffusion Cell Detection Growth Plate Mouse Retinex

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microwave apparatus to study the mechanical behavior of solid samples subjected to external stresses

Microwave apparatus to study the mechanical behavior of soli...

引用

实验力学国际会议

作者： F.Mango M.G.ascenzi G.Aschero S.Battaglia A.Benvenuti P.Gizdulich Physics Department and I.N.F.M. Via Buonarroti 2 56127 Pisa Italy Department of Orthopedic Surgery Biomechanics Research UCLA U.S.A. Physics Department and I.N.F.M. Via Buonarroti 2 56127 Pisa Italy I.I.R.G. Area di Ricerca C.N.R. Via Moruzzi 1 56011 Pisa Italy Dept. of Experimental Medicine and Pathology "La Sapienza" University 00161 Rome Italy Clinical Physiopathology Dept. University of Florene 50134 Florence Italy

＜正＞ We present a differential microwave apparatus to investigate the mechanical behavior of samples subjected to any stress which produces a change in the sample length. The device measures small displacements through the measurement of the resonance frequency variation of a microwave resonator compared to that of a reference resonator. The relative sensitivity （Δv/v） is about 10-8 in the resonance frequency of the resonator and corresponds to a （Δh/h） of about 7×10-8 in the height of the resonator, having such dimensions as to resonate in the TE011 mode in the X band. This means that changes in the sample length of some nanometers can be measured, whatever is the value of the sample length. The resolution of the device of some nanometers "single shot" looks high enough, however the good stability of the apparatus along long periods allows that periodic stresses and averaging may be applied, in this way the resolution becomes some picometers. The extended measurement range is another interesting feature of the apparatus, indeed, if the DC coupling is used, with the resolution limit less than one ten of nanometers, a displacement up to 100 micrometers can be recorded. The frequency response ranges from DC to some Hz.

关键词： Residual strain metals biological composites thermal cycling piezoelectricity dilatometer

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：