检索结果-南通市图书馆

A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera lucia Pereira-Chioccola Thelma Suely Okay lucia maria Almeida Braz Laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil Laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil Laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil Laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil Laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biodecoloration of Reactive Black 5 by the methylotrophic yeast Candida boidinii MM 4035

引用

Journal of Environmental Sciences 2017年第3期29卷 78-87页

作者： maria M.Martorell Hipo1ito F.Pajot Pablo M.Ahmed lucia I.C.de Figueroa Antarctic Argentinian Institute Balcarce 290CABAArgentina PROIMI-CONICET TucumánArgentina Department of Products Quality IV San Pablo University-TucumdnTucumánArgentina Agroindustrial Experimental Station Obispo Colombres TucumánArgentina Department of Superior Microbiology Faculty of BiochemistryChemistry and PharmacyNational University of TucumánTucumánArgentina

Azo dyes are extensively used in textile dyeing and other industries. Effluents of dying industries are specially colored and could cause severe damage to the environment. The anaerobic treatment of textile dying effluents is nowadays the preferred option, but it could generate carcinogenic aromatic amines. Recently, yeasts have become a promising altemative, combining unicellular growth with oxidative mechanisms. This work reports the characterization of the first methylotrophic yeast with dye decolorizing ability, Candida boidinii MM 4035 and some insights into its decoloration mechanism. The analysis of two selected media revealed a possible two stages mechanism of Reactive Black 5 decoloration. In glucose poor media, decoloration is incomplete and only the first stage proceeds, leading to the accumulation of a purple compound. In media with higher glucose concentrations, the yeast is able to decolorize totally an initial concentration of 200 mg/L. The entire process is co-metabolic, being largely dependent on glucose concentration but being able to proceed with several nitrogen sources. Manganese dependent peroxidase but not laccase activity could be detected during decoloration. Aromatic amines do not accumulate in culture media, supporting an oxidative decoloration mechanism of unknown ecophysiological relevance.

关键词： Biodecoloration DyesMethylotrophic yeasts Medium optimization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Management of hepatitis C in patients with chronic kidney disease

引用

World Journal of Gastroenterology 2015年第2期21卷 408-422页

作者： Roberto J Carvalho-Filho Ana Cristina CA Feldner Antonio Eduardo B Silva maria lucia G Ferraz Division of Gastroenterology Hepatology SectionFederal University of Sao PauloSao PauloSP 04023-900Brazil

Hepatitis C virus(HCV) infection is highly prevalent among chronic kidney disease(CKD) subjects under hemodialysis and in kidney transplantation(KT) recipients, being an important cause of morbidity and mortality in these patients. The vast majority of HCV chronic infections in the hemodialysis setting are currently attributable to nosocomial transmission. Acute and chronic hepatitis C exhibits distinct clinical and laboratorial features, which can impact on managementand treatment decisions. In hemodialysis subjects, acute infections are usually asymptomatic and anicteric; since spontaneous viral clearance is very uncommon in this context, acute infections should be treated as soon as possible. In KT recipients, the occurrence of acute hepatitis C can have a more severe course, with a rapid progression of liver fibrosis. In these patients, it is recommended to use pegylated interferon(PEG-IFN) in combination with ribavirin, with doses adjusted according to estimated glomerular filtration rate. There is no evidence suggesting that chronic hepatitis C exhibits a more aggressive course in CKD subjects under conservative management. In these subjects, indication of treatment with PEG-IFN plus ribavirin relies on the CKD stage, rate of progression of renal dysfunction and the possibility of a preemptive transplant. HCV infection has been associated with both liver disease-related deaths and cardiovascular mortality in hemodialysis patients. Among those individuals, low HCV viral loads and the phenomenon of intermittent HCV viremia are often observed, and sequential HCV RNA monitoring is needed. Despite the poor tolerability and suboptimal efficacy of antiviral therapy in CKD patients, many patients can achieve sustained virological response, which improve patient and graft outcomes. Hepatitis C eradication before KT theoretically improves survival and reduces the occurrence of chronic graft nephropathy, de novo glomerulonephritis and post-transplant diabetes mellitus.

关键词： Hepatitis C virus Chronic kidney disease End-stage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ontogenetic niche specialization of the spider crab Libinia ferreirae associated with the medusa Lychnorhiza lucerna

引用

Current Zoology 2022年第5期68卷 549-559页

作者： Geslaine Rafaela Lemos Gonçalves Milena Regina Wolf mariana Antunes Felipe Wanderley Amorim maria lucia Negreiros-Fransozo Antonio Leão Castilho Group of Studies on Crustacean Biology Ecology and Culture-NEBECCBiosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil and Laboratory of Pollination Ecology and Interactions-LEPI Biosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil

Symbiotic relationships in marine environments are not fixed and can change throughout the animal’s *** study investigated the ontogeny of symbiosis of the spider crab Libinia ferreirae with the host medusa Lychnorhiza *** described the type of relationship,the temporal correlation among species,and food *** than 50%of the sampled crabs were symbionts,most in early life *** highest number of crabs found in a single medusa was *** was observed throughout most of the year but was more evident in warm *** crab has many benefits in this relationship with a *** is the use of food resources captured by the medusa,primarily *** the crab steals the medusa’s food,it is a kleptoparasitic *** is a niche partition between symbiont and the free-living crabs as they occupy different habitats and use nonoverlapping food *** research reported that symbiosis first developed during the crab’s last larval phase(megalopa)when crab and medusa are in the same *** of the crab’s behavior shows that symbiosis occurs when the crab can grab to the medusa when the host touches the sea *** crab also took advantage of water currents,releasing itself from the substrate and then drifting toward the *** symbiotic relationship that crabs have with the medusa provides then with a nursery,food resources,shelter,dispersion,and decreased competition with free-living adult crabs,all essential for the crab’s survival.

关键词： behavior development kleptoparasitism megalopa niche segregation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bcl-2 down modulation in WEHI-3B/CTRES cells resistant to Cholera Toxin(CT)-induced apoptosis

引用

Cell Research 2006年第3期16卷 306-312页

作者： Augusto Pessina Cristina Croera Nicoletta Savalli Arianna Bonomi Loredana Cavicchini Elisa Turlizzi Fabiana Guizzardi lucia Guido Laura Daprai maria GraziaNeri Institute of Microbiology University of MilanVia Pascal 3620133 MilanItaly ECVAM Institute for Health and Consumer ProtectionEuropean Commission Joint Research CentreIspra 21020Italy UCLA Department of AnesthesiologyLos AngelesCA 90095USA

The very different effects of Cholera Toxin (CT) on cell growth and proliferation may depend on the type of ganglio-side receptors in cell membranes and different signal transduction mechanisms triggered,but other functions relatedto the drug resistance mechanisms can not be *** effect of CT treatment on the "in vitro" clonogenicity,thePopulation Doubling Time (PDT),apoptosis,PKA activation and Bax and Bcl-2 expression was evaluated in WEHI-3Bcell line and its CT-resistant subclone (WEHI-3B/CTRES).In WEHI-3B parental cells the dramatic accumulation ofcAMP induced by CT correlated well with PKA activation,increased PDT value,inhibition of clonogenicity and apop-tosis.H-89 treatment inhibited PKA activation by CT but did not protect the cells from apoptosis and growth *** WEHI-3B/CTRES no significant CT-dependent accumulation of cAMP occurred with any increase of PKA activityand *** CT resistant cells (WEHI-3B/CTRES),Bcl-2 expression was down regulated by both CT or drug treatment(eg.,ciprofloxacin,CPX) although these cells were protected from CT-dependent apoptosis but not from *** from other cell models described,down regulation of Bcl-2 is proved to be independent on cAMPaccumulation and PKA *** observations support the implication of cAMP dependent kinase (PKA) in theinhibition of WEHI-3B cells growth and suggest that,in WEHI-3B/CTRES,Bcl-2 expression could be modulated byCT in the absence of cAMP *** in consideration of many contradictory data reported in literature,ourcell models(of one sensitive parental cell strain and two clones with different uncrossed specific resistance to CT andCPX)provides a new and interesting tool for better investigating the relationship between the CT signal transductionmechanisms and Bcl-2 expression and function.

关键词： cholera toxin Bcl-2 PKA apoptosis ciprofloxacin resistance

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Sub-chronic effects of a Phthirusa pyrifolia aqueous extract on reproductive function and comparative hormone levels in male rats

引用

Asian Pacific Journal of Tropical Biomedicine 2016年第3期6卷 202-210页

作者： Romero Marcos Pedrosa Brandao-Costa Vivianne Ferreira Araujo Elizabeth Neves maria Tereza Santos Correia Ana lucia Figueiredo Porto maria das Gracas Carneiro-da-Cunha Department of Biochemistry Center of Biological Sciences Federal University of Pernambuco Keizo Asami Laboratory of Imunopathology LIKA/UFPE Department of Anatomy Federal University of Pernambuco Department of Animal Morphology and Physiology Federal Rural University of Pernambuco Keizo Asami Laboratory of Imunopathology LIKA/UFPE

Objective: To explore the effects of aqueous extract from Phthirusa pyrifolia leaves(67 mg/kg body weight for 12 days) on the reproductive function of male Wistar rats through oral ***: Animals(n = 30), aged 13 weeks and weighing(378.5 ± 5.0) g, were housed in a vivarium under controlled environmental conditions [photoperiod of 12 h light/dark,temperature of(23 ± 1)C] and were fed standard rations ad libitum. The experiment ran for 12 days, wherein animals were divided into three groups: negative control(n = 6)received water, positive control(n = 12) with finasteride at a concentration of 1.0 mg/kg;and a test group(n = 12) submitted to aqueous extract. At the end of the experiment, the animals were sacrificed and submitted to ***: The morphological results of the testes showed that the aqueous extract induced significant changes in the diameter and cross-sectional area of the seminiferous tubules as well as the thickness of the seminiferous epithelium. Furthermore, the extract was able to abruptly decrease testosterone concentrations by about 81.88% in the treated group when compared with the negative control,(47.0 ± 4.8) ng/d L and(255.0 ± 2.0) ng/d L,respectively, and 76.8%,(211.0 ± 8.7) ng/d L, when compared with finasteride. However,the extract causes neither liver damage nor impairment of renal ***: These results suggest that the high amounts of flavonoids shown to be in the extract may be responsible for its hepato-protective effects and suggest a possible decrease in the libido and reproduction of rats.

关键词： Reproductive function Phytochemical Testosterone Finasteride Phthirusa pyrifolia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virological and histological evaluation of intestinal samples in COVID-19 patients

引用

World Journal of Gastroenterology 2022年第44期28卷 6282-6293页

作者： Dajana Cuicchi Liliana Gabrielli maria lucia Tardio Giada Rossini Antonietta D’Errico Pierluigi Viale Tiziana Lazzarotto Gilberto Poggioli Department of Medical and Surgical Sciences Surgery of the Alimentary TractIRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Department of Experimental Diagnostic and Specialty MedicineMicrobiology UnitIRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Department of Experimental Diagnostic and Specialty MedicinePathology UnitIRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Department for Integrated Infectious Risk Management Infectious Diseases UnitIRCCS Azienda Ospedaliero-Universitaria di BolognaBologna 40138Italy Infectious Diseases Unit Department for Integrated Infectious Risk ManagementUniversity of BolognaBologna 40138Italy Section of Microbiology Department of ExperimentalDiagnostic and Specialty MedicineUniversity of BolognaBologna 40138Italy Department of Medical and Surgical Sciences University of BolognaBologna 40138Italy

BACKGROUND Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)is the pathogen responsible for pandemic coronavirus disease 2019(COVID-19).It is a highly contagious virus which primarily affects the respiratory tract,nevertheless,the lungs are not the only target organs of the *** intestinal tract could represent an additional tropism site for *** observations have collectively suggested that enteric infections can occur in COVID-19 ***,the detection of viral RNA in gastrointestinal(GI)tissue samples has not been adequately investigated and results are *** To detect the presence of SARS-CoV-2 RNA in intestinal mucosa samples and to evaluate histological *** The COVID-19 patients hospitalized at an Italian tertiary hospital from April 2020 to March 2021 were evaluated for enrollment in an observational,monocentric *** study population was composed of two groups of adult *** the first group(biopsy group,30 patients),patients were eligible for inclusion if they had mild to moderate disease and if they agreed to have a rectal biopsy;in the second group(surgical specimen group,6 patients),patients were eligible for inclusion if they underwent intestinal resection during index ***-nine intestinal mucosal samples were *** Viral RNA was not detectable in any of the rectal biopsies performed(0/53).Histological examination showed no enterocyte damage,but slight edema of the lamina propria with mild inflammatory lymphoplasmacytic *** was no difference in inflammatory infiltrates in patients with and without GI ***-CoV-2 RNA was detected in fecal samples in 6 cases out of 14 cases examined(42.9%).In the surgical specimen group,all patients underwent emergency intestinal *** RNA was detected in 2 surgical specimens of the 6 examined,both of which were from patients with active neoplastic *** examination also pointed

关键词： COVID-19 SARS-CoV-2 Intestinal infection Intestinal samples Intestinal tropism Rectal samples

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Elastography as a predictor of liver cirrhosis complications after hepatitis C virus eradication in the era of direct-acting antivirals

引用

World Journal of Hepatology 2021年第11期13卷 1663-1676页

作者： lucia Cerrito maria Elena Ainora Alberto Nicoletti Matteo Garcovich Laura Riccardi Maurizio Pompili Antonio Gasbarrini maria Assunta Zocco CEMAD Digestive Disease Center Fondazione Policlinico Universitario"A.Gemelli"IRCCSCatholic University of Rome(Italy)Rome 00168Italy

Chronic inflammation due to hepatitis C virus(HCV)infection leads to liver fibrosis and rearrangement of liver tissue,which is responsible for the development of portal hypertension(PH)and hepatocellular carcinoma(HCC).The advent of direct-acting antiviral drugs has revolutionized the natural history of HCV infection,providing an overall eradication rate of over 90%.Despite a significant decrease after sustained virological response(SVR),the rate of HCC and liver-related complications is not completely eliminated in patients with advanced liver *** the reasons are still unclear,cirrhosis itself has a residual risk for the development of HCC and other PH-related *** elastography is a recently developed non-invasive technique for the assessment of liver *** the achievement of SVR,liver stiffness(LS)usually decreases,as a consequence of reduced inflammation and,possibly,*** studies emphasized the application of LS assessment in the management of patients with SVR in order to define the risk for developing the complications of chronic liver disease(functional decompensation,gastrointestinal bleeding,HCC)and to optimize long-term prognostic outcomes in clinical practice.

关键词： Direct-acting antiviral agents Liver stiffness Portal hypertension Hepatocellular carcinoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A prospective study on radiofrequency ablation locally advanced pancreatic cancer

引用

Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 306-311页

作者： Riccardo Casadei Claudio Ricci Raffaele Pezzilli Carla Serra lucia Calculli Antonio maria Morselli-Labate Donatella Santini Francesco Minni Department of Surgery S.Orsola-Malpighi Hospital University of Bologna Department of Internal Medicine and Gastroenterology S.Orsola-Malpighi Hospital University of Bologna Department of Radiology S.Orsola-Malpighi Hospital University of Bologna Department of Internal Medicine and Gastroenterology Department of Pathology S.Orsola-Malpighi Hospital University of Bologna

BACKGROUND:Radiofrequency ablation(RFA)has been suggested as a new treatment option for patients with locally advanced *** study aimed to prospectively evaluate the efficacy and safety of intraoperative RFA in patients with unresectable,locally advanced,non-metastatic carcinoma of the pancreatic ***:RFA was the first step of the surgical procedure and was carried out on the mobilized pancreatic head followed by biliary by-pass and ***-and post-operative morbidity and mortality,performance status, pain control,quality of life,and survival at 24 months were ***:Seven patients(3 men and 4 women;median age 66 years,range 47-80 years)were studied and 4 were eligible for *** RFA procedure was carried out in 3 of the 4 patients;in one patient it was not carried out because of the upstaging of the *** all 3 patients RFA achieved complete necrosis of the lesion.A biliary fistula developed 7 days after the procedure in one patient;all 3 patients developed ascites 8.6 days(range 7-9 days)on average after *** patients died respectively,at 3,4,and 5 months after the ***:In our experience,RFA is a feasible procedure, but it presents a very high rate of postoperative ***,pain control,life quality and survival rate are *** few data suggest no impact on survival.

关键词： pancreatic neoplasms radiofrequency ablation survival efficacy safety

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reversible acetylation regulates vascular endothelial growth factor receptor-2 activity

引用

Journal of Molecular Cell Biology 2014年第2期8卷 116-127页

作者： Annalisa Zecchin lucia Pattarini maria Ines Gutierrez Miguel Mano Antonello Mai Sergio Valente Mike P. Myers Sergio Pantano Mauro Giacca Molecular Medicine Laboratory International Centre for Genetic Engineering and Biotechnology (ICGEB) Trieste Italy Istituto Pasteur-Fondazione cenci-Bolognetti Dipartimento di Chimica e Tecnologie del Farmaco Sapienza Universita' di Roma Rome Italy Protein Networks Laboratory International Centre for Genetic Engineering and Biotechno[ogy (ICGEB) Trieste Italy Biomolecular Simulations Group Institut Pasteur de Montevideo' Montevideo Uruguay U932 Immunity and Cancer Integrative Biology of Human Dendritic Cells and T Cells Laboratory Institute Curie Paris France

The tyrosine kinase receptor vascular endothelial growth factor receptor 2 （VEG FR2） is a key regulator of angiogenesis. Here we show that VEGFR2 is acetylated in endothelial cells both at four lysine residues forming a dense cluster in the kinase insert domain and at a single lysine located in the receptor activation loop. These modifications are under dynamic control of the acetyltransferase p300 and two deacetyiases HDAC5 and HDAC6. We demonstrate that VEGFR2 acetylation essentially regulates receptor phosphorylation. In par- ticular, VEGFR2 acetylation significantly alters the kinetics of receptor phosphorylation after ligand binding, allowing receptor phos- phoryiation and intraceUular signaling upon proLonged stimulation with VEGF. Molecular dynamics simulations indicate that acetylation of the lysine in the activation loop contributes to the transition to an open active state, in which tyrosine phosphorylation is favored by better exposure of the kinase target residues. These findings indicate that post-translational modification by acetyiation is a critical mechanism that directLy affects VEGFR2 function.

关键词： acetylation angiogenesis p300 phosphorylation vascular endothelial growth factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：