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Molecular Diagnosis of Thyroid Nodules and Its Future Implications for the Management of Thyroid Cancer

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International Journal of Otolaryngology and Head & Neck Surgery 2021年第5期10卷 398-418页

作者： Rodrigo Arrangoiz Jeronimo Garcialopez De Llano maria Fernanda Mijares Gonzalo Fernandez-Christlieb Vanitha Vasudevan Amit Sastry Adrian Legaspi Jennifer Fernandez Frank de la Cruz Fernando Cordera Daniel Margain Center for Advanced Surgical Oncology Steward Health Hialeah FL USA American British Cowdray Medical Center Mexico City Mexico

Molecular testing in thyroid nodules and thyroid cancer is rapidly evolving;care must be used when incorporating molecular testing for thyroid nodules into clinical practice. A clear appreciation of the goals and restraints of molecular testing must be integrated into how physicians use and explain molecular testing to patients. Molecular tests can help rule in cancer for indeterminate thyroid nodules with very specific mutations for thyroid cancer, such as BraF and RET/PTC, and can help reduce the rates of completion thyroidectomies in this era of de-escalation of the management of thyroid disease. The positive predictive value (PPV) of malignant cytology (Bethesda VI) is 98%;and even though molecular testing improves specificity and PPV, it falls short of this ideal for other mutations. We present a detailed evaluation of the current state of molecular testing and their clinical relevance in the setting of diagnostic utility and their impact on surgical decision-making. By recapitulating the clinical impact of these tests and some of the related drawbacks, we hope to provide adequate up to date information of the appropriate utilization of these tools in the management of indeterminate or suspicious thyroid nodules and highlight future directions on their utilization for the management of thyroid cancer.

关键词： Thyroid Cancer Papillary Thyroid Cancer Papillary Thyroid Microcarcinoma Thyroid Nodule Thyroid Cancer Treatment Molecular Studies for Thyroid Cancer Affirma Thyroseq

Bull spermatozoa selected by thermotaxis exhibit high DNA integrity,specific head morphometry,and improve ICSI outcome

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1001-1011页

作者： Sara Ruiz-Diaz Rosane Mazzarella Paula Navarrete-Lopez raul Fernandez-Gonzalez Celia de Frutos maria Maroto Claudia Cucala Paula Beltran-Brena Marta Lombo Dimitrios Rizos Alfonso Gutierrez-Adan Department of Animal Reproduction INIA-CSIC28040 MadridSpain Mistral Fertility Clinics S.L Clinica TambreCalle Tambre828002 MadridSpain

Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation(SDF)in mice,humans,and *** study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by *** We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of *** these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 ***,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially *** The results showed lower SDF(χ^(2),Prameters(length and width,Prated in comparison to those *** distribution of sperm subpopulations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated *** performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),P<0.05).Conclusion Our results showed that bull sperm selection by thermotaxis has a much higher D

关键词： ART Bovine Sperm morphometry Sperm selection Thermotaxis

Current Understanding of Papillary Thyroid Carcinoma

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International Journal of Otolaryngology and Head & Neck Surgery 2021年第3期10卷 184-221页

作者： Rodrigo Arrangoiz Jeronimo Garcialopez De Llano maria Fernanda Mijares Gonzalo Fernandez-Christlieb Vanitha Vasudevan Amit Sastry Adrian Legaspi Jennifer Fernandez Frank de la Cruz Fernando Cordera Daniel Margain Center for Advanced Surgical Oncology Florida USA American British Cowdray Medical Center Mexico City Mexico Sociedad Quirurgica S.C. at the American British Cowdray Medical Center Mexico City

The term thyroid neoplasm incorporates tumors that originate from follicular cells and those that arise from parafollicular cells (C cells). Differentiated thyroid cancer, which originates from follicular cells, includes papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), oncocytic cell carcinoma (Hürthle), poorly differentiated carcinoma, and anaplastic thyroid carcinoma (ATC). PTC tends to have an indolent clinical course with low morbidity and mortality. However, this entity has a broad range of biological and clinical behavior that can result in disease recurrence and death, depending on patient and tumor characteristics and the initial treatment approach. PTC is the most common form of well-differentiated thyroid cancer (WDTC) and based on the most recent statistics, accounts for approximately 89.4% of all thyroid malignancies. PTC appears as an irregular solid or cystic nodule in normal thyroid parenchyma. PTC has the propensity for lymphatic invasion, but it is less likely to have hematogenous spread. Around 11% of patients with PTC present with distant metastases outside the neck and mediastinum. This manuscript with review the current understanding of the epidemiology, pathology, molecular characteristics, prognostic factors, and dynamic risk stratification of PTC centered on an evidence-based and personalized approach.

关键词： Thyroid Cancer Papillary Thyroid Cancer Papillary Thyroid Microcarcinoma Thyroid Nodule Thyroid Cancer Treatment Molecular Studies for Thyroid Cancer

AtSYP51/52 Functions Diverge in the Post-Golgi Traffic and Differently Affect Vacuolar Sorting

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Molecular Plant 2013年第3期6卷 916-930页

作者： maria De Benedictis Gianluca Bleve marianna Faraco Egidio Stigliano Francesco Grieco Gabriella Piro Giuseppe Dalessandro Gian Pietro Di Sansebastiano Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali University of Salento Campus Ecotekne 73100 Lecce Italy Present address: Dipartimento di Bioscienze Universita degli Studi di Parma Parco Area delle Scienze 11/A 43124 Parma Italy Institute of Science of Food Production National Research Council via Provinciale Lecce-Monteroni 73100 Lecce Italy laboratory of Cell and Molecular Biology University of Neuchatel Rue Emile-Argand 11 CP 158 2009 Neuchatel Switzerland

Plant sensitive factor attachment protein receptors （SNAREs） encoded by genes of the same sub-family are generally considered as redundant in promoting vesicle-associated membrane fusion events. Nonetheless, the application of innovative experimental approaches highlighted that members of the same gene sub-family often have different functional specificities. In this work, two closely related Qc-SNAREs--the AtSYP51 and the AtSYP52--are compared in their ability to influence different secretory pathways. Their role in the vesicle sorting to the central vacuole has been revised and they were found to have a novel inhibitory function. When transiently overexpressed, the SYP51 and the SYP52 distributed between the TGN and the tonoplast. Our data demonstrate that these SYPs （syntaxin of plants） act as t-SNARE when present on the membrane of TGN/PVC, whereas they behave as inhibitory or interfering SNAREs （i-SNAREs） when they accumulate on the tonoplast. Moreover, the performed functional analysis indicated that the AtSYP51 and the AtSYP52 roles differ in the traffic to the vacuole. The findings are a novel contribution to the functional characterization of plant SNAREs that reveals additional non-fusogenic roles.

关键词： endocytosis endosome Golgi i-SNARE plant endomembranes protoplast SNARE TGN tonoplast vacuole.

Influence of surface roughness on the color of dental-resin composites

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第7期12卷 552-562页

作者： razvan GHINEA laura UGARTE-ALVAN Ana YEBra Oscar E.PECHO rade D.PAraVINA maria del Mar PEREZ Department of Optics Faculty of ScienceUniversity of Granada Department of Stomatology Faculty of OdontologyUniversity of Granada Houston Center for Biomaterials and Biomimetics & Department of Restorative Dentistry and Biomaterials University of Texas Dental Branch at Houston

This study deals with the influence of surface roughness on the color of resin *** resin composites (microfilled,hybrid,and microhybrid) were each polished with 500-grit,1 200-grit,2 000-grit,and 4 000-grit SiC *** roughness parameter (ra) was measured using a Plμ confocal microscope,and field-emission scanning electron microscope (Fe-SEM) images were used to investigate filler *** was measured using a spectroradiometer and a D65 standard illuminant (geometry diffuse/0° specular component excluded (SCE) mode).Surface roughness decreased with grit number and was not influenced by filler size or size distribution.A significant influence of ra on lightness (L*) was *** increased with decreases in roughness,except for specimens that underwent polishing procedure 4 (PP4;500-grit,1 200-grit,2 000-grit,and 4 000-grit SiC papers consecutively).Generally,it was found that surface roughness influenced the color of resin *** composites that underwent PP1 (500-grit SiC paper) exhibited significant differences in chroma (C*),hue (h°),and lightness (L*) compared to composites that underwent PP3 (500-grit,1 200-grit,and 2 000-grit SiC papers consecutively) and *** difference (E*) between the polishing procedures was within acceptability thresholds in dentistry.

关键词： Color measurement Dental resin composites Roughness

Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

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Journal of Integrative Plant Biology 2009年第11期51卷 982-992页

作者： Shaohong Qu Jong-Seong Jeont Pieter B.F. Ouwerkerk maria Bellizzi Jan Leach Pamela Ronald Guo-Liang Wang Department of Plant Pathology Ohio State University Columbus OH 43210 USA Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Department of Plant Pathology University of California Davis CA 95616 USA Graduate School of Biotechnology and Plant Metabolism Research Center Yongin 446-701 Korea Institute of Biology Leiden University Clusius Laboratory RA Leiden 2300 The Netherlands Bioagdcultural Sciences and Pest Management Colorado State University Fort Collins CO 80523 USA Crop Gene Engineering Key laboratory of Hunan Province and Pre-State Key laboratory of Crop Germplasm Renovation and Resource Utilization Hunan Agricultural University Changsha 410128 China

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches：（i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,

关键词： Ac-Ds transposable element glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain-inducible expression Cre-lox site-specific recombination rice.

Disaster Risk Management Through the Design Safe Cyberinfrastructure

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International Journal of Disaster Risk Science 2020年第6期11卷 719-734页

作者： Jean-Paul Pinelli maria Esteva Ellen M.rathje David Roueche Scott J.Brandenberg Gilberto Mosqueda Jamie Padgett Frederick Haan Department of Mechanical and Civil Engineering Florida Institute of TechnologyMelbourneFL 32901USA Texas Advanced Computing Center University of Texas at AustinAustinTX 78712USA Department of Civil Engineering University of Texas at AustinAustinTX 78712USA Department of Civil Engineering Auburn UniversityAuburnAL 36849USA Department of Civil and Environmental Engineering University of California in Los AngelesLos AngelesCA 90095USA Department of Structural Engineering University of California in San DiegoLa JollaCA 92093USA Department of Civil and Environmental Engineering Rice UniversityHoustonTX 77005USA Engineering Department Calvin CollegeGrand RapidsMI 49546USA

Design Safe addresses the challenges of supporting integrative data-driven research in natural hazards *** is an end-to-end data management,communications,and analysis platform where users collect,generate,analyze,curate,and publish large data sets from a variety of sources,including experiments,simulations,field research,and post-disaster *** achieves key objectives through:(1)integration with high performance and cloud-computing resources to support the computational needs of the regional risk assessment community;(2)the possibility to curate and publish diverse data structures emphasizing relationships and understandability;and(3)facilitation of real time communications during natural hazards events and disasters for data and information *** resultant services and tools shorten data cycles for resiliency evaluation,risk modeling validation,and forensic *** article illustrates salient features of the *** summarizes its design principles,architecture,and *** focus is on case studies to show the impact of Design Safe on the disaster risk *** Next Generation Liquefaction project collects and standardizes case histories of earthquake-induced soil liquefaction into a relational database—Design Safe—to permit users to interact with the *** can correlate in Design Safe building dynamic characteristics based on data from building sensors,with observed damage based on ground motion *** groups upload,curate,and publish wind,seismic,and coastal damage data they gather during field reconnaissance missions,so these datasets are available shortly after a *** a part of the education and community outreach efforts of Design Safe,training materials and collaboration space are also offered to the disaster risk management community.

关键词： Cyberinfrastructure Design Safe Disaster risk management Natural hazard data management

Inflammation, lipid metabolism and cardiovascular risk in rheumatoid arthritis: A qualitative relationship?

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世界骨科杂志：英文版 2014年第3期5卷 304-311页

作者： Carmen García-Gómez maria Bianchi Diana de la Fuente Lina Badimon Teresa Padró Emili Corbella Xavier Pintó Department of Rheumatology Consorci Sanitari de Terrassa (CST)Unitat Investigació Biomèdica CST-UAB (Universitat Autònoma de Barcelona)08227-TerrassaBarcelonaSpain Department of Rheumatology Hospital de Viladecans08840-ViladecansBarcelonaSpain Cardiovascular Research Center CSIC-ICCCBiomedical Research Institute Sant-Pau08025-BarcelonaSpain Lipid and Vascular Risk Unit Department of Internal MedicineHospital Universitari de Bellvitge-IDIBELLL’Hospitalet de LlobregatBarcelona University08907 FipecCIBERobn ISCIIISpain

Life expectancy in patients with rheumatoid arthritis(ra)is reduced compared to the general population owing to an increase in cardiovascular diseases(CVD)not fully explained by traditional cardiovascular risk *** recent years,interest has been focused on the alterations in lipid metabolism in relation to chronic inflammation as one of the possible mechanisms involvedin the pathogenesis of atherosclerosis of ra *** regarding this issue has revealed quantitative alterations in lipoproteins during the acute-phase reaction,and has also demonstrated structural alterations in these lipoproteins which affect their functional *** many alterations in lipid metabolism have been described in this regard,these structural changes associated with inflammation are particularly important in high-density lipoproteins as they affect their cardioprotective *** this respect,excessive oxidation in low-density lipoprotein(LDL)and increased lipoprotein(a)with a predominance of smaller apolipoprotein(a)isoforms has also been *** article will discuss proinflammatory high-density lipoproteins(pi HDL),oxidized LDL and lipoprotein(a).Elevated concentrations of these lipoproteins with marked pro-atherogenic properties have been observed in ra patients,which could help to explain the increased cardiovascular risk of these patients.

关键词： Rheumatoid arthritis Cardiovascular disease Lipoproteins Proinflammatory high-density lipoproteins Lipoprotein(a) Oxidized low-density lipoproteins Lipid metabolism Inflammation

Enterobius vermicularis infection among population of General Mansilla,Argentina

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World Journal of Gastroenterology 2004年第17期10卷 2535-2539页

作者： Betina C Pezzani Marta C Minvielle maria M de Luca María A Córdoba María C Apezteguía Juan A Basualdo Cátedra de Microbiología y Parasitología Facultad de Ciencias MédicasUniversidad Nacional de La Plata1900Argentina Comisi6n de Investigaciones Científicas de la provincia de Buenos Aires

AIM:To evaluate the relationships between the personal, sociocultural,and environmental characteristics,and the presence or absence of symptoms with the detection of Enterobius vermicularis(***)in a population sample in our region(General Mansilla,Province of Buenos Aires,Argentina),by individual and familiar analyses. METHODS:*** was diagnosed in 309 people from 70 family units residing in the urban area and the rural area of the city of General *** of them was surveyed so as to register personal,environmental and sociocultural *** about the presence or absence of anal itch,abdominal pain and sleeping disorder were also *** associations were determined by square chi *** regression models were adjusted by using a backward conditional stepwise method to determine the presence of this parasite in the individuals and in the families. RESULTS:The parasites were found in 29.12%(90/309) of the individuals,with a frequency of 14.28%(20/140) among the heads of the families and of 41.42%(70/169) among the *** only variables showing a significant association were affiliation,where the risk category was “being the son/daughter of”,and the symptoms were abdominal pain,sleeping disorder,and anal *** with a member infected with parasite were considered Positive Families(PF)and they were 40/70(57.14%),only 5%(2/40)of the PF had 100% of their members infected with the *** logistic regression models applied showed that the risk categories were mainly affiliation(son/daughter) and housing(satisfactory)among others. CONCLUSION:The presence of *** proved in one third of the studied *** frequency of families with all their members infected with the parasite was very *** of the studied personal,sociocultural, and environmental variables did not turn out to be significantly associated with the presence of the *** association with the category of“son/daught

关键词： Enterobius Abdominal Pain Adult Anal Canal Animals Argentina Child Enterobiasis Family Health Female Humans Male Pruritus Research Support, Non-U.S. Gov't Risk Factors Rural Population Sanitation Socioeconomic Factors Urban Population