检索结果-南通市图书馆

Study of the Production of Lentinus crinitus(L.)*** Enzymes Grown on Agro-Industrial Waste

Advances in Bioscience and Biotechnology 2017年第8期8卷 259-272页

作者： Taiana de Araújo Conceicao maria gabriela bello koblitz Hélio Mitoshi Kamida Aristóteles Góes-Neto Health Sciences Center Universidade Federal do Reconcavo da BahiaSanto Antonio de JesusBrazil Food Technology Department Universidade Federal do Estado do Rio de JaneiroRio de JaneiroBrazil Biological Sciences Department Universidade Estadual de Feira de SantanaFeira de SantanaBrazil Biological Sciences Institute Universidade Federal de Minas GeraisBelo HorizonteBrazil

Exclusively Brazilian, the Caatinga biome has been thus far a place of very few studies on the Basidiomycetes fungi. Due to its semiarid climate, fungi found in the region are likely to carry lignolytic enzymes which hold biotechnological potential to be used in industrial processes of agro-industrial residue bioconversion. This study performed a response surface statistical planning to optimize the secretion of enzymes such as laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) by Lentinus crinitus. Three variables were under analysis: different concentrations of barley and cassava residue, pH and temperature. MnP enzyme showed the highest enzymatic activity rate (23.5 IU/L). Additionally, MnP had the best results of enzyme secretion for substrate composition of 50% barley and 50% cassava, at pH 7 and temperature at 28°C for a 28-day incubation period. However, further studies are pivotal to test the efficiency in lignin bioconversion by the enzymes synthesized in this work and also to establish their usage pattern on a large scale.

关键词： Lignolytic Enzymes Lentinus Agro Waste

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Production of Manganese Peroxidase by Trametes villosa on Unexpensive Substrate and Its Application in the Removal of Lignin from Agricultural Wastes

引用

Advances in Bioscience and Biotechnology 2014年第14期5卷 1067-1077页

作者： Marilia Lordelo Cardoso Silva Volnei Brito de Souza Veronica da Silva Santos Helio Mitoshi Kamida Joao Ronaldo T.de Vasconcellos-Neto Aristoteles Goes-Neto maria gabriela bello koblitz Technology Department State University of Feira de Santana(UEFS)Feira de SantanaBrasil Biology Department State University of Feira de Santana(UEFS)Feira de SantanaBrasil Food Technology Department Federal University of the State of Rio de Janeiro(UNIRIO)Rio de JaneiroBrasil

Manganese peroxidase (MnP) is a ligninolytic enzyme that is involved in the removal of lignin from the cell wall of plants. This removal facilitates the access of hydrolytic enzymes to the carbohydrate polymers that are hydrolyzed to simple sugars, which allows the subsequent fermentation to obtain bioproducts, such as ethanol. In this work, response surface methodology (RSM) was employed to optimize the culture conditions on unexpensive substrate for MnP secretion by Trametes villosa. Three independent variables were evaluated (i.e., temperature, moisture content and pH). The crude extract containing MnP was used in the delignification experiment and it caused a reduction in lignin content for all residues tested: 35.05 ± 1.45 (%) for the sugar cane bagasse;63.11 ± 0.06 (%) for the sisal fiber and 39.61 ± 0.39 (%) for the coconut shell, under the reaction conditions tested after 4 hours of fermentation. The preliminary results exhibited the potential application of this enzyme in the removal of lignin from plant residues. However, the conditions should be evaluated and optimized for each residue type.

关键词： Ligninolytic Enzymes Basidiomycete Sugarcane Bagasse Coconut Shell Sisal Fiber

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：