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Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos Santos Zamarioli Michele Rosana Maia Santos Adolfo Garcia Erustes Vanessa Marques Meccatti Thais Cristine Pereira Soraya S Smaili maria cristina marcucci Carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(P<0.05).The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts(P<0.05).Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(P<0.05).The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone(P<0.01).HEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(P<0.01).Furthermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

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Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine Pereira Lara Steffany de Carvalho Letícia Ferreira dos Santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos maria cristina marcucci Amjad Abu Hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

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Botanical Aspects,Caffeine Content and Antioxidant Activity of Coffea arabica

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American Journal of Plant Sciences 2019年第6期10卷 1013-1021页

作者： Andreia Marques dos Santos Luis Carlos Marques Carolina Passarelli Goncalves maria cristina marcucci Post-Graduation Program in Pharmacy and Biotechnology of the Anhanguera University of Sao Paulo OsascoBrazil Pharmacy Department of Anhanguera University of Sao Paulo OsascoBrazil

Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink from Coffea arabica species, samples grown in the city of Ourinhos, the third most productive region in the state of Sao Paulo, a study of its properties and characteristics was conducted. The pharmacobotanical characteristics were investigated performed according to usual techniques in these researches through macroscopic and microscopic studies through cross-sections. The oil obtained for analysis was extracted by soxhlet, and the caffeine content was measured for green grains using High Performance Liquid Chromatography (HPLC) using as stationary phase, column C18 and gradient mobile phase formed by 80% water and 20% methanol. The concentration obtained was 44.983 ± 0.86 μg/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC50 25.89 ± 1.16 μg/mL.

关键词： Coffea arabica Botanical Characterization Caffeine

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Biodegradability of bioplastics in different aquatic environments:A systematic review

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Journal of Environmental Sciences 2024年第8期142卷 169-181页

作者： maria cristina Lavagnolo Valentina Poli Anna maria Zampini Valentina Grossule Department of Civil Environmental and Architectural Engineering-Laboratory of Environmental EngineeringUniversity of PadovaLungargine Rovetta 8Padova 35100Italy

Bioplastics were first introduced as environmentally friendly materials,with properties similar to those of conventional plastics.A bioplastic is defined as biodegradable if it can be decomposed into carbon dioxide under aerobic degradation,or methane and CO_(2)under anaerobic conditions,inorganic compounds,and new cellular biomass,by the action of naturally occurring *** definition however does not provide any information on the environmental conditions,timescale and extent at which decomposition processes should *** regard to the aquatic environment,recognized standards have been established to assess the ability of plastics to undergo biodegradation;however,these standards fail to provide clear targets to be met to allow labelling of a bioplastic as ***,these standards grant the user an extensive leeway in the choice of process *** these reasons,the comparison of results deriving from different studies is *** authors analysed and discussed the degree of biodegradability of a series of biodegradable bioplastics in aquatic environments(both fresh and salt water)using the results obtained in the laboratory and from on-site testing in the context of different research *** Oxygen Demand(BOD),CO_(2)evolution,surface erosion and weight loss were the main parameters used by researchers to describe the percentage of *** results showed a large variability both in weight loss and BOD,even when evaluating the same type of *** confirms the need for a reference range of values to be established with regard to parameters applied in defining the biodegradability of bioplastics.

关键词： Biodegradation Bioplastic Plastic Natural environment Plastic waste

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Flavonoid Contents and Antioxidant Activity in Fruit,Vegetables and Other Types of Food

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Agricultural Sciences 2015年第4期6卷 426-435页

作者： Juliana cristina Pereira Calado Paula Adriana Albertao Erica Aparecida de Oliveira Mario Henrique Sisto Letra Alexandra Christine Helena Frankland Sawaya maria cristina marcucci Post-Graduated School in Pharmacy Anhanguera University of Sao Paulo(UNIAN-SP)Sao PauloBrazil Department of Plant Biology and Pharmacy Course Institute of BiologyUniversidade Estadual de CampinasUNICAMPCampinasBrazil

Flavonoids are a class of natural polyphenolic compounds which cannot be synthesized by humans. These substances possess a series of biological properties, acting on biological systems as antioxidants. The purpose of this study was to analyze the properties of certain foods, determining the total flavonoids as well as their antioxidant activity and fat concentration. We evaluated several foods purchased at the local market, with respect to its antioxidant activity, using two experimental models, the discoloration of DPPH˙ radical and ABTS-. Some foods such as pitanga showed antioxidant activity. The lipid content of fatty foods like aaí, cacao and cupuaú was determined. Other foods, including pitanga, aaí, cacao and cupuaú, were evaluated for flavonoid content and antioxidant activity using multivariate statistical analysis (PCA) as a statistical tool to evaluate the correlation between these two parameters. As samples with ED50 up to 500 μg/mL show promising antioxidant activity, several Brazilian fruit and vegetables could be consumed to this end, with a good correlation between flavonoid content and antioxidant activity in most samples. The daily dose of different types of food for antioxidant activity has been calculated based on these results.

关键词： Amount of Lipids Antioxidant Activity Chemometric Analysis Fat Content Flavonoids Food Fruit Vegetables

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

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Journal of Integrative Medicine 2019年第2期17卷 132-140页

作者： Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia Adolfo Erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo J.S.Pereira André Hinsberger Ezequiel P.Viriato maria cristina marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto Departamento de Farmacologia Escola Paulista de Medicina (EPM) Universidade Federal de Sao Paulo (UNIFESP) Grupo de Fitocomplexos e Sinalizacao Celular Escola de Ciências da Saúde Universidade Anhembi Morumbi Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz Laboratório de Produtos Naturais e Quimiometria Programa de pós-graduacao em Farmácia e Biotecnologia Universidade Anhanguera de Sao Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual de Campinas (UNICAMP) Unidade de Oncologia Experimental EPM Universidade Federal de Sao Paulo (UNIFESP)

Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its *** study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast ***: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo ***: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated ***: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy.

关键词： Angelica archangelica Umbelliferae Apoptosis Cell death Breast cancer Angelicin

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Quantification of Flavonoids in Brazilian Orange Peels and Industrial Orange Juice Processing Wastes

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Agricultural Sciences 2017年第7期8卷 631-644页

作者： Regina M.S.Pereira Begona Giménez-Cassina Lopez Susana N.Diniz Alyne Alexandrino Antunes Daniel Moreno Garcia Carlos Rocha Oliveira maria cristina marcucci Post-Graduated School in Pharmacy and Biotechnology Anhanguera University of Sao Paulo—UNIAN-SPSao PauloBrazil Department of Plant Biology and Pharmacy Course Institute of BiologyUniversity of CampinasUNICAMPCampinasBrazil Centro Interdisciplinar de Investigacao Bioquimica Universidade de Mogi das Cruzes—UMCMogi das CruzesBrazil Departamento de Farmacologia Escola Paulista de MedicinaUniversidade Federal de Sao PauloSao PauloBrazil Escola de Ciencias da Saude Universidade Anhembi MorumbiSao PauloBrazil

The flavonoid content in orange peels of different Brazilian citrus varieties such as bahia, lima, lima-of-persian, morcote, pera, ponkan, seleta, cravo, kinkan and pomelo was assessed. Industry processing juice wastes such as bagasse, bagasse residues, animal feeding bagasse, pulp WEUE and CORE-wash were also analyzed. The HPLC analysis indicates that the most abundant flavonoids found in these Brazilian citrus peels are hesperidin and naringin. The solvents used are selective for flavonoid extraction, and depending on their polarity, glycoside or aglycone flavonoids are extracted. The use of multivariate analysis shows that DMSO is the best solvent to extract glycosides flavanones while hexane displays high selectivity in the extraction of polymethoxylated flavones. The flavonoids present in the orange wastes, obtained at different stages of the industrial processing, are qualitative and quantitatively different. The identification and quantification of the flavonoid composition in each Brazilian citrus variety were evaluated and allowed the selection of the best solvent for the extraction of each specific class of flavonoids. These compounds were found to be more abundant in the fruit peels than in their juices, revealing their great industrial potential. The residual portion of the processing juices is also rich in flavonoids, depending on the processing step.

关键词： Citrus Orange Peel Flavonoid Brazilian Orange Orange Juice Processing Wastes

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In‑depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1027-1055页

作者： maria A.Gil Josep M.Cambra Heriberto Rodriguez‑Martinez cristina Cuello Inmaculada Parrilla Emilio A.Martinez Department of Medicine and Animal Surgery International Excellence Campus for Higher Education and Research“Campus Mare Nostrum”University of MurciaMurciaSpain Institute for Biomedical Research of Murcia(IMIB-Pascual Parrilla) MurciaSpain Technical University of Munich MunichGermany Department of Biomedical&Clinical Sciences(BKV) BKH/Obstetrics&GynaecologyLinkoping UniversityLinkopingSweden

Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo *** this study,the proteome of the endometrium and cho-rioallantoic membrane was characterized in pregnant sows(PS)during early gestation(d 18 and 24 of gestation)and in the endometrium of non-pregnant sows(NPS)during the same days using LC-MS/MS *** UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks,*** Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS,respectively;of these,1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS,*** addition,we iden-tified 3,968 proteins in the extraembryonic membranes of *** analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization,suggesting they dominated the moment of endometrial remodeling,implantation and adhesion of the lining *** are available via Pro-teomeXchange with identifier *** This is the first in-depth proteomic characterization of the endometrium and extraembryonic mem-branes during weeks 3 to 4 of gestation;data that contribute to the molecular understanding of the dynamic environ-ment during this critical period,associated with the majority of pregnancy losses.

关键词： Endometrium Extraembryonic membranes Implantation Pig Proteome

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Renewable Polymers in Biomedical Applications:From the Bench to the Market

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Journal of Renewable Materials 2024年第4期12卷 643-666页

作者： Rauany cristina Lopes Tamires Nossa Wilton Rogério Lustri Gabriel Lombardo maria Inés Errea Eliane Trovatti Department of Health and Biological Sciences University of Araraquara—UNIARAAraraquara14801-340Brazil Laboratory of Materials(LabMat)and Laboratory of Biopolymers(LaBioPol) Federal Institute of EducationScience and Technology of São Paulo Campus Itapetininga(IFSP)Itapetininga18.202‑000Brazil Instituto Tecnológico de Buenos Aires Cidade Autônoma de Buenos AiresC1437FBCArgentina Conselho Nacional de Pesquisas Científicas e Técnicas(CONICET) Cidade Autônoma de Buenos AiresC1425FQBArgentina Tecnologia YPF(Y-TEC) BerissoBuenos Aires1923Argentina

Polymers from renewable resources have been used for a long time in biomedical applications and found an irreplaceable role in some of *** uses have been increasing because of their attractive properties,contributing to the improvement of life quality,mainly in drug release systems and in regenerative *** using natural polymer,nano and microscale particles preparation,composites,blends and chemical modification strategies have been used to improve their properties for clinical *** many studies have been carried out with these natural polymers,the way to reach the market is long and only very few of them become commercially *** cellulose,bacterial cellulose,chitosan,poly(lactic acid)and starch can be found among the most studied polymers for biological applications,some with several derivatives already established in the market,and others with potential for *** this scenario this work aims to describe the properties and potential of these renewable polymers for biomedical applications,the routes from the bench to the market,and the perspectives for future developments.

关键词： Polymers renewable biomedical applications market

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High Amounts of Halogenated Natural Products in Sperm Whales(Physeter macrocephalus)from Two Italian Regions in the Mediterranean Sea

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Environment & Health 2024年第4期2卷 233-242页

作者： Sina Schweizer Kristin Halder Annika Schafer Jakob Hauns Letizia Marsili Sandro Mazzariol maria cristina Fossi Juan Muñoz-Arnanz Begoña Jiménez Walter Vetter Institute of Food Chemistry Department of Food Chemistry(170b)University of Hohenheim70599 StuttgartGermany European Union Reference Laboratory(EURL)for Halogenated POPs in Feed and Food 79114 FreiburgGermany Department of Environmental Earth and Physical SciencesUniversity of Siena53100 SienaItaly Department of Public Health Comparative Pathology and Veterinary HygieneUniversity of Padova35020 LegnaroItaly Department of Instrumental Analysis and Environmental Chemistry Institute of Organic Chemistry(IQOG-CSIC)28006 MadridSpain

Halogenated natural products(HNPs)are considered to be emerging contaminants whose environmental distribution and fate are only incompletely ***,several persistent and bioaccumulative HNP groups,together with manmade polychlorinated biphenyls(PCBs)and polybrominated diphenyl ethers(PBDEs),were quantified in the blubber of nine sperm whales(Physeter macrocephalus)stranded on the coast of the Mediterranean Sea in *** naturally occurring polybrominated hexahydroxanthene derivatives(PBHDs;sum of TetraBHD and TriBHD)were the most prominent substance class with up to 77,000 ng/g *** mean PBHD content(35,800 ng/g blubber)even exceeded the one of PCBs(28,400 ng/g blubber),although the region is known to be highly contaminated with manmade *** on mean values,Q1∼PBDEs>MeO-BDEs∼2,2′-diMeO-BB 80 and several other HNPs followed with decreasing *** blubber samples contained an abundant compound whose molecular formula(C_(16)H_(19)Br_(3)O_(2))was verified using high-resolution mass *** only plausible matching isomer was(2S,4′S,9R,9′S)-2,7-dibromo-4′-bromomethyl-1,1-dimethyl-2,3,4,4′,9,9′-9,9′-hexahydro-1H-xanthen-9-ol(OH-TriBHD),a hydroxylated secondary metabolite previously detected together with TriBHD and TetraBHD in a sponge known to be a natural producer of *** estimated mean amount of the presumed OH-TriBHD was 3000 ng/g blubber,which is unexpectedly high for hydroxylated compounds in the lipids of marine mammals.

关键词： Halogenated natural product naturally occurring polyhalogenated compound persistent organic pollutant sperm whale Italy polar metabolite

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