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complexed amino acid minerals vs. bis-glycinate chelated minerals:Impact on the performance of old laying hens

Animal Nutrition 2024年第1期16卷 395-408页

作者： marcos J.B.Santos maria c.m.m.ludke Leandro m.Silva carlos B.V.Rabello mércia R.Barros Fabiano S.costa clariana S.Santos Jamille S.S.Wanderley Department of Animal Science Rural Federal University of Pernambuco Department of Veterinary Science Rural Federal University of Pernambuco

The present study was to evaluate the effect of trace minerals(Zn, mn, and cu) from complexed amino acid minerals(ZmcAA) and bis-glycinate chelated minerals(ZmcGly) in laying hen diets on performance,internal and external egg quality, yolk mineral deposition, intestinal morphometry, and bone characteristics. From 78 to 98 weeks of age, 400 White LSL-Lite strain laying hens were distributed in a randomized design with 4 treatments with 10 replicates per treatment. Treatments were distributed in a 2 ×2 factorial arrangement using either Zn, mn, and cu of ZmcAA or ZmcGly source at 2 levels: low(20, 20,and 3.5 mg/kg of Zn, mn, and cu, respectively) or high(40, 40, and 7 mg/kg of Zn, mn, and cu,respectively). The analysis of variance was performed, and in cases where differences were observed, the means were compared using Tukey?s test(P ce and level of trace mineral supplementation had a significant impact on the performance of laying hens. Hens fed ZmcAA had higher egg production(P = 0.01), egg weight(P = 0.02), egg mass(P = 0.01), and lower feed conversion ratio(P =0.05) compared to those fed ZmcGly. The ZmcAA supplementation showed higher albumen height(P =0.01), albumen weight(P = 0.01), and eggshell thickness(P mn(Pcu(P ceived ZmcAA. Tibia weight(P = 0.04)and bone densitometry(P mcAA supplementation. In the small intestine, ZmcAA resulted in longer villi(P = 0.02) and shorter crypt depth(P = 0.01) in the *** and ileum measurements were influenced by the level and source of trace minerals(P mcAA exhibited superior performance, egg quality, deposition of trace minerals in the egg yolk, and bone density compared to hens fed ZmcGly. In this study, older laying hens supplemented with ZmcAA at lower levels demonstrated adequate levels of supplementation.

关键词： Bis-glycinates metal-amino acids Radiodensity Trace minerals

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Transcriptional reprogramming during human osteoclast differentiation identifies regulators of osteoclast activity

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Bone Research 2024年第1期12卷 180-198页

作者： morten S.Hansen Kaja madsen maria Price Kent Søe Yasunori Omata mario m.Zaiss caroline m.Gorvin morten Frost Alexander Rauch molecular Endocrinology Laboratory(KmEB) Department of EndocrinologyOdense University HospitalDK-5000 Odense CDenmark Department of clinical Research Faculty of Health SciencesUniversity of Southern DenmarkDK-5000 Odense CDenmark clinical cell Biology Pathology Research UnitDepartment of Clinical ResearchUniversity of Southern DenmarkDK-5000 Odense CDenmark Institute of metabolism and Systems Research(ImSR)and centre for Diabetes Endocrinology and Metabolism(CEDAM)University of BirminghamBirmingham B152TTUK centre for membrane Proteins and Receptors(cOmPARE) Universities of Birmingham and NottinghamBirmingham B152TTUK Department of molecular medicine University of Southern DenmarkDK-5000 Odense CDenmark Department of Orthopedic Surgery Faculty of MedicineThe University of TokyoTokyo 113-8655Japan Department of Internal medicine 3 Rheumatology and ImmunologyFriedrich-Alexander-University Erlangen-Nürnberg(FAU)and Universitätsklinikum ErlangenD-91054 ErlangenGermany Deutsches Zentrum Immuntherapie(DZI) Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum ErlangenD-91054 ErlangenGermany Steno Diabetes center Odense Odense University HospitalDK-5000 Odense CDenmark

Enhanced osteoclastogenesis and osteoclast activity contribute to the development of osteoporosis,which is characterized by increased bone resorption and inadequate bone *** novel antiosteoporotic therapeutics are needed,understanding the genetic regulation of human osteoclastogenesis could help identify potential treatment *** study aimed to provide an overview of transcriptional reprogramming during human osteoclast *** were differentiated from cD14+monocytes from eight female *** sequencing during differentiation revealed 8980 differentially expressed genes grouped into eight temporal patterns conserved across *** patterns revealed distinct molecular functions associated with postmenopausal osteoporosis susceptibility genes based on RNA from iliac crest biopsies and bone mineral density *** analyses revealed mutual dependencies between temporal expression patterns and provided insight into subtype-specific transcriptional *** donor-specific expression patterns revealed genes at the monocyte stage,such as filamin B(FLNB)and oxidized low-density lipoprotein receptor 1(OLR1,encoding LOX-1),that are predictive of the resorptive activity of mature *** expression of differentially expressed G-protein coupled receptors was strong during osteoclast differentiation,and these receptors are associated with bone mineral density SNPs,suggesting that they play a pivotal role in osteoclast differentiation and *** regulatory effects of three differentially expressed G-protein coupled receptors were exemplified by in vitro pharmacological modulation of complement 5 A receptor 1(c5AR1),somatostatin receptor 2(SSTR2),and free fatty acid receptor 4(FFAR4/GPR120).Activating c5AR1 enhanced osteoclast formation,while activating SSTR2 decreased the resorptive activity of mature osteoclasts,and activating FFAR4 decreased both the number and resorptive activity of mature *** conclus

关键词： osteoclast programming identif

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Blood metal concentrations and cardiac structure and function in total joint arthroplasty patients

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World Journal of Orthopedics 2024年第8期15卷 773-782页

作者： Peter c Brennan Stephanie m Peterson Thomas J O'Byrne mariana L Laporta cody c Wyles Paul J Jannetto Garvan c Kane maria Vassilaki Hilal maradit Kremers Department of Orthopedic Surgery Mayo ClinicRochesterMN 55905United States Department of Quantitative Health Sciences Mayo ClinicRochesterMN 55905United States Department of Anesthesiology and Perioperative medicine Mayo ClinicRochesterMN 55905United States Department of Laboratory medicine and Pathology Mayo ClinicRochesterMN 55905United States Department of cardiovascular Diseases Mayo ClinicRochesterMN 55905United States

BAcKGROUND There is concern regarding potential long-term cardiotoxicity with systemic distribution of metals in total joint arthroplasty(TJA)*** To determine the association of commonly used implant metals with echocardiographic measures in TJA *** The study comprised 110 TJA patients who had a recent history of high chromium,cobalt or titanium *** underwent two-dimensional,three-dimensional,Doppler and speckle-strain transthoracic echocardiography and a blood draw to measure metal *** and sex-adjusted linear and logistic regression models were used to examine the association of metal concentrations(exposure)with echocardiographic measures(outcome).RESULTS Higher cobalt concentrations were associated with increased left ventricular end-diastolic volume(estimate 5.09;95%cI:0.02-10.17)as well as left atrial and right ventricular dilation,particularly in men but no changes in cardiac *** titanium concentrations were associated with a reduction in left ventricle global longitudinal strain(estimate 0.38;95%cI:0.70 to 0.06)and cardiac index(estimate 0.08;95%cI,-0.15 to-0.01).cONcLUSION Elevated cobalt and titanium concentrations may be associated with structural and functional cardiac changes in some *** studies are warranted to better understand the systemic effects of metals in TJA patients.

关键词： Total joint arthroplasty metal-on-metal cardiotoxicity Heart failure Echocardiography cobalt

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A short climatology of black and brown carbon and their sources at a suburban site impacted by smoke in Brazil

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Journal of Environmental Sciences 2024年第2期136卷 498-511页

作者： Admir creso Targino Patricia Krecl Gabriel Yoshikazu Oukawa Eva maria mollinedo Graduate Program in Environmental Engineering Federal University of TechnologyAv.Pioneiros 3131LondrinaPR 86036-370Brazil Department of Environmental Engineering Federal University of TechnologyAv.Pioneiros 3131LondrinaPR 86036-370Brazil

Emissions from biomass burning challenge efforts to curb air pollution in cities downwind of fire-prone regions,as they contribute large amounts of brown carbon(Br c)and black carbon(Bc)*** investigated the patterns of Br c and Bc concentrations using Aethalometer data(atλ=370 and 880 nm,respectively)spanning four years at a site impacted by the outflow of *** data required to be post processed for the shadowing effect since,without correction,concentrations would be between 29%and 35%*** Br c concentrations were consistently higher than the Bc concentrations,indicating the prevalence of aerosols from biomass *** results were supported by the Angstrom coefficient(A_(370/880)),with values predominantly larger than 1(mean±standard deviation:1.25±0.31).A_(370/880)values below 1 were more prevalent during the wet season,which suggests a contribution from fossil fuel *** observed sharp Br c and Bc seasonal signals,with mean minimum concentrations of 0.40μg/m^(3)and 0.36μg/m^(3),respectively,in the wet season,and mean maximum concentrations of 2.05μg/m^(3)and 1.53μg/m^(3)in the dry *** largest concentrations were observed when northerly air masses moved over regions with a high density of fire *** burning of residential solid waste and industrial combustion caused extreme Br c and Bc concentrations under favourable wind *** neither pollutant is included in any ambient air quality standards,our results suggest that transboundary smoke may hamper efforts to meet the World Health Organization guidelines for fine particles.

关键词： Light-absorbing carbonaceous matter Black carbon Wildfires Biomass burning Aethalometer

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Impact of multifocal gas-permeable lens designs on short-term choroidal response, axial length, and retinal defocus profile

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 247-256页

作者： muteb Alanazi Patrick caroline Amane Alshamrani maria Liu Optometry Department College of Applied Medical SciencesKing Saud UniversityRiyadh 14813Saudi Arabia college of Optometry Pacific UniversityForest GroveOregon 97116-1756United States Optometry School University of California at BerkeleyCalifornia 94720United States

AIm:To investigate the impact of multifocal gas permeable contact lens(mFGPcL)in various add power and distance/near area allocation on short-term changes of choroidal thickness(chT),axial length(AL),and retinal defocus profile in young ***:Seventeen young adults(2 males and 15 females;age 23.17±4.48y)were randomly assigned to wear two designs binocularly with a one-week washout period in *** of four mFGPcL designs were *** designs were distance-center that varied in two add power(+1.50 and 3.00 D)and/or two distance zone(DZ)diameters(1.50 and 3.00 mm;design A:DZ 1.5/add 3.0,B:DZ 1.5/add 1.5,c:DZ 3.0/add 3.0,D:DZ 3.0/add 1.5).chT,AL,and peripheral refraction data were collected on each subject at baseline,on days 1 and 7 of mFGPcL daily *** was assessed in four quadrants using a spectraldomain optical coherence ***:AL was shortened by-26±44μm with lens c,-18±27μm with lens D,-13±29μm with lens A,and-8±30μm with lens B(all Pcant overall increase in chT was observed with all 4 designs(lens A:+6±6μm,B:+3±7μm,c:+8±7μm,and D:+8±7μm).Temporal and superior choroid exhibited more choroidal thickening associated with *** designs induced significant relative peripheral myopia(RPm)beyond the central 20o across the horizontal meridian in both nasal and temporal fields(PcONcLUSION:mFGPcLs show a significant influence on chT and AL,which are associated with significant increase in RPm after short-term *** reliability and feasibility of quantifying short-term changes in chT support its use as a promising marker for the long-term efficacy of myopia-controlling treatments.

关键词： choroid gas-permeable contact lens retinal defocus axial length myopia

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male genitourinary schistosomiasis-related symptoms among long-term Western African migrants in Spain: a prospective population-based screening study

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贫困所致传染病(英文) 2024年第2期13卷 29-38页

作者： Roure Sílvia Vallès Xavier Pérez-Quílez Olga López-mu?oz Israel chamorro Anna Abad Elena Valerio Lluís Soldevila Laura Espa?a Sergio Hegazy Alaa H. A. Fernández-Rivas Gema Gorriz Ester Herena Dolores Oliveira mário miralles maria carme conde carmen montero-Alia Juan José Fernández-Pedregal Elia miranda-Sánchez Jose Llibre Josep m. Isnard mar Bonet Josep maria Estrada Oriol Prat Núria clotet Bonaventura The Schisto-Stop study group Primary Health care Unit mataró-(Rocafonda-Palau) Barcelona North Metropolitan Health Directorate Catalan Institute for Health Camí Ral el Ravalet Mataró Barcelona Spain Barcelona North metropolitan Primary care Directorate Catalan Institute for Health Ctra. de Barcelona Sabadell Barcelona Spain Directorate for Innovation and Interdisciplinary cooperation Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain Irsicaixa-AIDS Research Institute Hospital Universitari Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Infectious Diseases Directorate Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain International Health Program (PROSIcS) Barcelona North Metropolitan Territorial Directorate for Infectious Diseases Catalan Institute for Health CAP La Salut Passatge dels Encants S/N Badalona Spain Fundació Lluita contra les Infeccions c/Can Ruti s/n Badalona Spain Infectious Diseases Department Hospital Universitari Germans Trias i Pujol c/Can Ruti s/n Badalona Spain Germans Trias i Pujol Research Institute c/Can Ruti s/n Badalona Spain microbiology Department Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Department of Genetics and microbiology Universitat Autònoma de Barcelona c/Can Ruti s/n Badalona Spain Faculty of medicine University of Asyut El Fateh Assiut Governorate Egypt Department of Urology Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain canovelles Primary Health care Unit Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Indústria Canovelles Spain

Background Schistosomiasis is highly endemic in sub-Saharan Africa and frequently imported to Europe. male urogenital manifestations are often neglected. We aimed to ascertain the prevalence of genitourinary clinical signs and symptoms among long-term African migrants in a non-endemic European country using a serology *** We carried out a prospective, community-based cross-sectional study of adult male migrants from sub-Saharan Africa living in *** serology tests and microscopic urine examinations were carried out, and clinical data were obtained from an electronic medical record search and a structured *** We included 388 adult males, mean age 43.5 years [Standard Deviation (SD) = 12.0, range: 18–76]. The median time since migration to the European Union was 17 [Interquartile range (IQR): 11–21] years. The most frequent country of origin was Senegal (N = 179, 46.1%). Of the 338, 147 (37.6%) tested positive forSchistosoma. Parasite eggs were present in the urine of only 1.3%. Nine genitourinary clinical items were significantly associated with positiveSchistosoma serology results: pelvic pain (45.2%;OR = 1.57, 95%cI: 1.0–2.4), pain on ejaculation (14.5%;OR = 1.85, 95%cI: 1.0–3.5), dyspareunia (12.4%;OR = 2.45, 95%cI: 1.2–5.2), erectile dysfunction (9.5%;OR = 3.10, 95%cI: 1.3–7.6), self-reported episodes of infertility (32.1%;OR = 1.69, 95%cI: 1.0–2.8), haematuria (55.2%;OR = 2.37, 95%cI: 1.5–3.6), dysuria (52.1%;OR = 2.01, 95%cI: 1.3–3.1), undiagnosed syndromic STIs (5.4%), and orchitis (20.7%;OR = 1.81, 95%cI: 1.0–3.1). clinical signs tended to *** Urogenital clinical signs and symptoms are prevalent among male African long-term migrants with a positiveSchistosoma serology results. Genital involvement can be frequent even among those with long periods of non-residence in their sub-Saharan African countries of origin. Further research is needed to develop diagnostic tools and validate therapeutic approaches to

关键词： Schistosomiasis chronic schistosomiasis Urogenital schistosomiasis male genital schistosomiasis Longterm migrant

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A multi-shot target wheel assembly for high-repetition-rate, laser-driven proton acceleration

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High Power Laser Science and Engineering 2024年第2期12卷 87-94页

作者： J.Penas A.Bembibre D.cortina-Gil L.martin A.Reija c.Ruiz m.Seimetz A.Alejo J.Benlliure Instituto Galego de Fisica de Altas Enerxias Universidade de Santiago de CompostelaSantiago de CompostelaSpain Laboratorio Laser de Aceleracion y Aplicaciones RIAIDTUniversidade de Santiago de CompostelaSantiago de CompostelaSpain Instituto Universitario de Física Fundamental y matematicas y Dpto.de Didactica de la matematica y de las ciencias Experimentales Universidad de SalamancaSalamancaSpain Instituto de Instrumentación para Imagen molecular(I3m) CSIC-Universitat Politecnica de ValenciaValenciaSpain

A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are *** assembly is based on a multi-target rotating wheel capable of hosting more than 5000 targets,mounted on a 3D motorized stage to allow rapid replenishment and alignment of the target material between laser *** automatic alignment procedure consists of a detailed mapping of the impact positions at the target surface prior to the irradiation that ensures stable operation of the target,which alongside the purpose-built design of the target wheel,enables operation at rates up to 10 *** and continuous laser-driven proton acceleration at 10 Hz is demonstrated,with observed cut-off energy stability about 15%.

关键词： high repetition rate laser-plasma acceleration multi-shot operation proton source target assembly

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calcium-sensitive protein mLc1 as a possible modulator of the astrocyte functional state

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Neural Regeneration Research 2025年第7期20卷 2008-2010页

作者： Elena Ambrosini Angela Lanciotti maria Stefania Brignone Department of Neuroscience Istituto Superiore di SanitàRomeItaly

Astrocytes,the main population of glial cells in the central nervous system(cNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental stage to adult *** maintain the optimal functionality of the brain,astroglial cells are particularly committed to reacting to every change in tissue homeostatic conditions,from mild modifications of the physiological environment,a process called astrocyte activation,to the more severe alterations occurring in pathological situations causing astrocyte reactivity or reactive astrogliosis(Escartin et al.,2021).During these reactive states,astrocytes mount an active,progressive response encompassing morphological,molecular,and interactional remodeling,leading to the acquisition of new functions and the loss of others,whose intensity,duration,and reversibility are dependent on the nature of the stimulus and regulated in a context-specific manner.

关键词： alterations modulator maintain

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Plasticity of photorespiratory carbon concentration mechanism in Sedobassia sedoides(Pall.)Freitag&*** under elevated cO_(2)concentration and salinity

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Journal of Arid Land 2024年第7期16卷 963-982页

作者： Zulfira RAKHmANKULOVA Elena SHUYSKAYA maria PROKOFIEVA Kristina TODERIcH Pavel VORONIN Timiryazev Institute of Plant Physiology Russian Academy of SciencesMoscow 127276Russia Graduate School of Bioresources Mie University1577 KurimamachiyaTsu 514-8507Japan

Rising atmospheric cO_(2)(carbon dioxide)concentrations and salinization are manifestations of climate change that affect plant growth and *** with an intermediate c_(3)-c_(4)type of photosynthesis live in a wide range of precipitation,temperature,and soil quality,but are more often found in warm and dry *** of the intermediate c_(3)-c_(4)photosynthetic type is c_(2)photosynthesis with a carbon concentration mechanism(ccm)that reassimilates cO_(2)released via ***,the ecological significance under which c_(2)photosynthesis has advantages over c_(3)and c_(4)plants remains largely *** tolerance and functioning of ccm were studied in plants from two populations(P1 and P2)of Sedobassia sedoides(Pall.)Freitag&*** *** with c_(2)photosynthesis exposed to 4 d and 10 d salinity(200 mm Nacl)at ambient(785.7 mg/m^(3),acO_(2)and elevated(1571.4 mg/m^(3),ecO_(2))cO_(2).On the fourth day of salinity,an increase in Na+content,activity catalase,and superoxide dismutase was observed in both populations.P2 plants showed an increase in proline content and a decrease in photosynthetic enzyme content:rubisco,phosphoenolpyruvate carboxylase(PEPc),and glycine decarboxylase(GDc),which indicated a weakening of c_(2)and c_(4)characteristics under *** under 10 d salinity led to an increased Na^(+)content and activity of cyclic electron flow around photosystem I(PSI cEF),a decreased content of K^(+)and GDc in both populations.P1 plants showed greater salt tolerance,which was assessed by the degree of reduction in photosynthetic enzyme content,PSI cEF activity,and changes in relative growth rate(RGR).Differences between populations were evident under the combination of ecO_(2)and *** long-term salinity and ecO_(2),more salt-tolerant P1 plants had a higher dry biomass(DW),which was positively correlated with PSI cEF *** less salt-tolerant P2 plants,DW correlated with transpiration and dark

关键词： photosystemsⅠandⅡ carbon-concentrating mechanism glycine decarboxylase rubisco phosphoenolpyruvate carboxylase(PEPc) cyclic electron flow salinity stress drylands

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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane mazzarella Karina canon‑Beltran Yulia N.cajas meriem Hamdi Encina m.Gonzalez Juliano c.da Silveira claudia L.V.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of Biochemistry and molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LmGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LmGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, mAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes mammalian Preimplantation Reproductive fluids

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