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A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species

Horticulture Research 2016年第1期3卷 30-42页

作者： Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis Larisa Garkava-Gustavsson Diego Micheletti marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg Department of Biosciences University of MilanMilan20133Italy Plant Breeding Wageningen University and ResearchWageningen6700AJThe Netherlands Research and Innovation Centre Edmund Mach FoundationSan Michele all’Adige38010Italy Biometris Wageningen University and ResearchWageningen6700AAThe Netherlands IRHS INRAAGROCAMPUS-OuestUniversitéd’AngersSFR 4207 QUASAVBeaucouzé49071France Department of Agricultural Sciences University of BolognaBologna40127Italy Department of Molecular Biology Laimburg Research Centre for Agriculture and ForestryOra39040Italy Department of Plant Breeding Swedish University of Agricultural SciencesAlnarp23053Sweden Hendrix Genetics Research Technology&ServicesBoxmeer 5830 ACThe Netherlands

Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome *** apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of *** approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of *** map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 ***,chromosome 5 was oriented according to its homoeologous chromosome *** map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL *** collinearity with the genome sequences v1 and v3 are *** our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and *** result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species.

关键词： breeding linkage mapping

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The SUPER reporting guideline suggested for reporting of surgical technique

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Hepatobiliary Surgery and Nutrition 2023年第4期12卷 534-544,I0024-I0026页

作者： Kaiping Zhang Yanfang Ma Jinlin Wu Qianling Shi Leandro Cardoso Barchi marco Scarci Rene Horsleben Petersen Calvin S.H.Ng Steven Hochwald Ryuichi Waseda Fabio Davoli Robert Fruscio Giovanni Battista Levi Sandri Michel Gonzalez Benjamin Wei Guillaume Piessen Jianfei Shen Xianzhuo Zhang Panpan Jiao Yulong He Nuria M.Novoa Benedetta Bedetti Sebastien Gilbert Alan D.L.Sihoe Alper Toker Alfonso Fiorelli Marcelo F.Jimenez Toni Lerut Aung Y.Oo Grace S.Li Xueqin Tang Yawen Lu Hussein Elkhayat Tomaz Stupnik Tanel Laisaar Firas Abu Akar Diego Gonzalez-Rivas Zhanhao Su Bin Qiu Stephen D.Wang Yaolong Chen Shugeng Gao Editorial Office AME Publishing CompanyHong KongChina School of Chinese Medicine Hong Kong Baptist UniversityHong KongChina Department of Cardiovascular Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouChina Evidence-based Medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhouChina Lanzhou University Institute of Health Data Science LanzhouChina Digestive Surgery Division Department of GastroenterologyUniversity of Sao Paulo School of MedicineSão PauloBrazil Department of Cardiothoracic Surgery Imperial College Healthcare NHS TrustLondonUK Department of Cardiothoracic Surgery University Hospital of CopenhagenRigshospitaletCopenhagenDenmark The Chinese University of Hong Kong Prince of Wales HospitalShatinNew TerritoriesHong KongChina Department of Surgical Oncology Roswell Park Cancer InstituteBuffaloNYUSA Department of General Thoracic Breast and Pediatric SurgeryFukuoka UniversityFukuokaJapan Department of Thoracic Surgery AUSL RomagnaS.Maria delle Croci Teaching HospitalRavennaItaly Clinic of Obstetrics and Gynecology University of Milan-BicoccaSan Gerardo HospitalMonzaItaly Division of General Surgery and Liver Transplantation S.Camillo HospitalRomeItaly Service of Thoracic Surgery Lausanne University Hospital(CHUV)LausanneSwitzerland Division of Cardiothoracic Surgery Department of SurgeryUniversity of Alabama BirminghamBirminghamALUSA University of Lille CNRSInsermCHU LilleUMR9020-U1277-CANTHER-Cancer Heterogeneity Plasticity and Resistance to TherapiesLilleFrance Department of Cardiothoracic Surgery Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhaiChina The First School of Clinical Medicine Lanzhou UniversityLanzhouChina Thoracic Surgery Service University Hospital of SalamancaBiomedical Institute of SalamancaSalamancaSpain Department of Thoracic Surgery Helios Clinic Bonn/Rhein SiegBonnGermany Division of Thoracic Surgery Department of SurgeryThe Ottawa

Background:Existing reporting guidelines pay insufficient attention to the detail and comprehensiveness reporting of surgical *** Surgical techniqUe rePorting chEcklist and standaRds(SUPER)aims to address this gap by defining reporting standards for surgical *** SUPER guideline intends to apply to articles that encompass surgical technique in any study design,surgical discipline,and stage of surgical ***:Following the EQUATOR(Enhancing the QUAlity and Transparency Of health Research)Network approach,16 surgeons,journal editors,and methodologists reviewed existing reporting guidelines relating to surgical technique,reviewed papers from 15 top journals,and brainstormed to draft initial items for the *** initial items were revised through a three-round Delphi survey from 21 multidisciplinary Delphi panel experts from 13 countries and *** final SUPER items were formed after an online consensus meeting to resolve disagreements and a three-round wording refinement by all 16 SUPER working group members and five SUPER ***:The SUPER reporting guideline includes 22 items that are considered essential for good and informative surgical technique *** items are divided into six sections:background,rationale,and objectives(items 1 to 5);preoperative preparations and requirements(items 6 to 9);surgical technique details(items 10 to 15);postoperative considerations and tasks(items 16 to 19);summary and prospect(items 20 and 21);and other information(item 22).Conclusions:The SUPER reporting guideline has the potential to guide detailed,comprehensive,and transparent surgical technique reporting for *** may also assist journal editors,peer reviewers,systematic reviewers,and guideline developers in the evaluation of surgical technique papers and help practitioners to better understand and reproduce surgical technique.

关键词： Surgical technique surgical innovation reporting guideline reporting checklist Surgical techniqUe rePorting chEcklist and standaRds(SUPER)

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用一个P沟和N沟MOS场效应管组成的逆变器级

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电力电子 2011年第5期9卷 19-22页

作者： marco Purschel 赵宏嵩郝锐周元钧 Infineon Technologies AG Germany 北京航空航天大学

现在的汽车上有很多电力驱动装置。从车窗的起落装置到电动助力转向系统,各种不同的动力都是由不同的电机和电子元器件组合来提供和实现的。用来驱动这些应用装置的变换器一般是由几个N沟道场效应管,一个驱动集成电路,一个微处理器,和... 详细信息

现在的汽车上有很多电力驱动装置。从车窗的起落装置到电动助力转向系统,各种不同的动力都是由不同的电机和电子元器件组合来提供和实现的。用来驱动这些应用装置的变换器一般是由几个N沟道场效应管,一个驱动集成电路,一个微处理器,和通信与电源设备组成。典型的应用是驱动电动机的功率开关,只有一个H桥拓扑或是三相桥结构。作为微处理器和功率场效应管的接口,驱动电路将提供给场效应管的栅极必要的电流和电压,以保证它达到安全和预期的开关性能。针对汽车对于安全性方面的较高要求,一些保护功能也被纳入到驱动电路中,比如短路保护和过温保护。本文主要介绍一种新的方法,即用一个P沟道MOS场效应管作为高压端开关,而一个N沟道MOS场效应管作为低压端开关。先介绍其优点,再分析它的缺点。此外,本文还介绍P/N沟道组合的驱动集成电路,此电路现在市场上没有出现。

关键词：电力驱动装置电源设备

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软特性650V IGBT:低电磁干扰和电压尖峰的优化器件

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变频器世界 2011年第7期 81-83页

作者： Wilhelm Rusche Andreas Hrtl marco Bssler 英飞凌科技股份公司

近年来,功率半导体厂商致力于提高器件的开关速度,这带来了开关损耗降低和系统能效提升的益处。这些功率器件需要优化的直流电路寄生电感(L_s)。为了满足具有大电流的高功率应用的需求,推出了一种全新的芯片650V IGBT4,旨在提供更大的... 详细信息

近年来,功率半导体厂商致力于提高器件的开关速度,这带来了开关损耗降低和系统能效提升的益处。这些功率器件需要优化的直流电路寄生电感(L_s)。为了满足具有大电流的高功率应用的需求,推出了一种全新的芯片650V IGBT4,旨在提供更大的设计自由度。这款全新的IGBT4器件具备更好的关断软度,并且由于关断电流变化率d i/dt的降低,带来了更低的关断电压尖峰。

关键词： IGBT 电磁干扰电压尖峰优化器件

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Laparoscopic management of intra-abdominal infections:Systematic review of the literature

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World Journal of Gastrointestinal Surgery 2015年第8期7卷 160-169页

作者： Federico Coccolini Cristian Tranà Massimo Sartelli Fausto Catena Salomone Di Saverio Roberto Manfredi Giulia Montori marco Ceresoli Chiara Falcone Luca Ansaloni General Surgery Department Papa Giovanni XXIII Hospital General and Emergency Surgery Department Macerata Hospital General and Emergency Surgery Department Maggiore Hospital General and Trauma Surgery Department Maggiore Hospital

AIM: To investigate the role of laparoscopy in diagnosis and treatment of intra abdominal ***: A systematic review of the literature was performed including studies where intra abdominal infections were treated ***: Early laparoscopic approaches have become the standard surgical technique for treating acute cholecystitis. The laparoscopic appendectomy has been demonstrated to be superior to open surgery in acute appendicitis. In the event of diverticulitis, laparoscopic resections have proven to be safe and effective procedures for experienced laparoscopic surgeons and may be performed without adversely affecting morbidity and mortality rates. However laparoscopic resection has not been accepted by the medical community as the primary treatment of choice. In high-risk patients, laparoscopic approach may be used for exploration or peritoneal lavage and drainage. The successful laparoscopic repair of perforated peptic ulcers for experienced surgeons, is demonstrated to be safe and effective. Regarding small bowel perforations, comparative studies contrasting open and laparoscopic surgeries have not yet been conducted. Successful laparoscopic resections addressing iatrogenic colonic perforation have been reported despite a lack of literature-based evidence supporting such procedures. In post-operative infections, laparoscopic approaches may be useful in preventing diagnostic delay and controllingthe ***: Laparoscopy has a good diagnostic accuracy and enables to better identify the causative pathology; laparoscopy may be recommended for the treatment of many intra-abdominal infections.

关键词： Laparoscopy Post-operative Treatment Perforation A

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Same-day 2-L PEG-citrate-simethicone plus bisacodyl vs split 4-L PEG: Bowel cleansing for late-morning colonoscopy

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World Journal of Gastrointestinal Endoscopy 2013年第9期5卷 433-439页

作者： Annalisa de Leone Darina Tamayo Giancarla Fiori Davide Ravizza Cristina Trovato Giuseppe De Roberto Linda Fazzini marco Dal Fante Cristiano Crosta Division of Endoscopy European Institute of Oncology Gastroenterology and Endoscopy Department

AIM: To evaluate the efficacy, tolerability, acceptability and feasibility of bisacodyl plus low volume polyethyleneglycol-citrate-simeticone(2-L PEG-CS) taken the same day as compared with conventional split-dose 4-L PEG for late morning colonoscopy. METHODS: Randomised, observer-blind, parallel group, comparative trial carried out in 2 centres. Out patients of both sexes, aged between 18 and 85 years, undergoing colonoscopy for diagnostic investigation, colorectal cancer screening or follow-up were eligible. The PEG-CS group received 3 bisacodyl tablets(4 tablets for patients with constipation) at bedtime and 2-L PEG-CS in the morning starting 5 h before colonoscopy. The control group received a conventional 4-L PEG formulation given as split regimen; the morning dose was taken with the same schedule of the low volume preparation. The Ottawa Bowel Preparation Scale(OBPS) score was used as the main outcome ***: A total of 164 subjects were enrolled and 154 completed the study; 78 in the PEG-CS group and 76 in the split 4-L PEG group. The two groups were comparable at baseline. The OBPS score in the PEG-CS group(3.09 ± 2.40) and in the PEG group(2.39 ± 2.55) were equivalent(difference +0.70; 95%CI:-0.09-1.48). This was confirmed by the rate of successful bowel cleansing in the PEG-CS group(89.7%) and in the PEG group(92.1%)(difference-2.4%; 95%CI:-11.406.70). PEG-CS was superior in terms of mucosa visibility compared to PEG(85.7% vs 72.4%, P = 0.042). There were no significant differences in caecum intubation rate, time to reach the caecum and withdrawal time between the two groups. The adenoma detection rate was similar(PEG-CS 43.6% vs PEG 44.7%). No serious adverse events occurred. No difference was found in tolerability of the bowel preparations. Compliance was equal in both groups: more than 90% of subjects drunk the whole solution. Willingness to repeat the same bowel preparations was about 90% for both regimes. CONCLUSION: Same-day PEG-CS is feasib

关键词： Bowel preparation Polyethyleneglycol Simethicone Ottawa Bowel Preparation Scale Colonoscopy

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Synthesis and Characterization of TiO2 Nanoreservoirs Supported with Platinum II and Sodium Bicarbonate Solution

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材料科学与工程（中英文A版） 2015年第1期5卷 100-108页

作者： Jose Albino Moreno Rodriguez Genaro Carmona Gutierrez marco-Antonio Gonzalez Coronell Efrain Rubio Rosas Lilian-Aurora Moreno Rodrlguez Fernando Moreno Rodriguez Chemistry Department Faculty of Chemistry Science Academic Entailment Center and Technology Transfer Physics Institute C. U. Colonia San Manuel. Puebla Puebla. C. P. 72570. Mexico Tapia's Chiautla Hospital S/N C.P. Puebla 74730. Mexico

In the past 10 years, the nanoreservoirs to based on TiO2 have taken the lead to develop new and better ways to control and possible "removal" of diseases that have bewildered mankind since its existence. In seeking to contribute to these expectations, at this early stage we synthesized nanoreservoirs of TiO2 with sodium bicarbonate solution to 15% (NaHCO3-15/TiO2), with salt of platinum(II) to 0.1% (Pt-0.1/TiO2) and with the NaHCO3 15% and the platinum(II) 0.1% "in situ" (Pt-0.1-NaHCO3-15/TiO2) by sol-gel. We show that TiO2 and Pt-0.1/TiO2 nanoreservoirs are amorphous. While the NaHCO3-15/TiO2 and the Pt-0.1-NaHCO3-15/TiO2 nanoreservoirs are crystalline. The NaHCO3-15/TiO2 and Pt-0.1-NaCO3-15/TiO2 nanoreservoirs are crystalline because to transformation of the sodium bicarbonate (NaHCO3) to trona phase. The trona phase increases the average particle size of the Pt-0.1-NaCO3-15/TiO2 nanoreservoirs of 180 nm to 130 nm (NaHCO3-15/TiO2). The nanoreservoirs doped with platinum decreases the particle size to 50 nm (Pt-0.1/TiO2).

关键词：二氧化钛碳酸氢钠溶液合成 TiO2 二代白金表征

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FAIR Principles:Interpretations and Implementation Considerations

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Data Intelligence 2020年第1期2卷 10-29,293-302,322页

作者： Annika Jacobsen Ricardo de Miranda Azevedo Nick Juty Dominique Batista Simon Coles Ronald Cornet Melanie Courtot Merce Crosas Michel Dumontier Chris T.Evelo Carole Goble Giancarlo Guizzardi Karsten Kryger Hansen Ali Hasnain Kristina Hettne Jaap Heringa Rob W.W.Hooft Melanie Imming Keith G.Jeffery Rajaram Kaliyaperumal Martijn GKersloot Christine R.Kirkpatrick Tobias Kuhn Ignasi Labastida Barbara Magagna PeterMcQuilton Natalie Meyers Annalisa Montesanti Mirjam van Reisen Philippe Rocca-Serra Robert Pergl Susanna-Assunta Sansone Luiz Olavo Bonino da Silva Santos Juliane Schneider George Strawn Mark Thompson Andra Waagmeester Tobias Weigel Mark D.Wilkinson Egon L.Willighagen Peter Wittenburg marco Roos Barend Mons Erik Schultes Leiden University Medical Center Leiden2333 ZAThe Netherlands Institute of Data Science Maastricht UniversityUniversiteitssingel 60Maastricht 6229 ERThe Netherlands Department of Computer Science The University of ManchesterOxford RoadManchester M139PLUK Oxford e-Research Centre Department of Engineering SciencesUniversity of OxfordOxford OX13PJUK School of Chemistry Faculty of Engineering and Physical SciencesUniversity of SouthamptonSO171BJUK Amsterdam UMC University of AmsterdamAmsterdam 1000 GGThe Netherlands European Bioinformatics Institute(EMBL-EBI) HinxtonCambridgeCB101SDUK Harvard University CambridgeMassachusetts 02138USA Department of Bioinformatics–BiGCaT NUTRIMMaastricht UniversityMaastricht 6229 ERThe Netherlands Conceptual and Cognitive Modeling Research Group(CORE) Free University of Bozen-BolzanoBolzano 39100Italy Aalborg University Aalborg DK-9220Denmark Insight Centre for Data Analytics National University of Ireland GalwayH91 TK33Ireland Centre for Digital Scholarship Leiden University LibrariesLeiden2333 ZAThe Netherlands Department of Computer Science Vrije Universiteit AmsterdamDe Boelelaan 11051081 HV AmsterdamThe Netherlands Dutch Techcentre for Life Sciences(DTL) UtrechtThe Netherlands SURF Utrecht 3511 EPThe Netherlands Keith G Jeffery Consultants FaringdonUK Castor EDC Paasheuvelweg 25Wing 5D1105 BPAmsterdamThe Netherlands San Diego Supercomputer Center University of California San DiegoLa JollaCalifornia 92093USA Learning and Research Resources Centre(CRAI) Universitat de Barcelona08007 BarcelonaSpain Environment Agency Austria A-1090 ViennaAustria University of Notre Dame 75004 ParisFrance Health Research Board(HRB) Dublin 2DO2 H638Ireland Liacs Institute of Advanced Computer Science Leiden University2311 GJ LeidenThe Netherlands Czech Technical University in Prague Faculty of Information Technology(FIT CTU)16000 Prague 6Czech Republic GO FAIR International Support&Coordination Office(GFISCO) LeidenThe Netherlands Harvard Catalys

The FAIR principles have been widely cited,endorsed and adopted by a broad range of stakeholders since their publication in *** intention,the 15 FAIR guiding principles do not dictate specific technological implementations,but provide guidance for improving Findability,Accessibility,Interoperability and Reusability of digital *** has likely contributed to the broad adoption of the FAIR principles,because individual stakeholder communities can implement their own FAIR ***,it has also resulted in inconsistent interpretations that carry the risk of leading to incompatible ***,while the FAIR principles are formulated on a high level and may be interpreted and implemented in different ways,for true interoperability we need to support convergence in implementation choices that are widely accessible and(re)-*** introduce the concept of FAIR implementation considerations to assist accelerated global participation and convergence towards accessible,robust,widespread and consistent FAIR *** self-identified stakeholder community may either choose to reuse solutions from existing implementations,or when they spot a gap,accept the challenge to create the needed solution,which,ideally,can be used again by other communities in the ***,we provide interpretations and implementation considerations(choices and challenges)for each FAIR principle.

关键词： FAIR guiding principles FAIR implementation FAIR convergence FAIR communities choices and challenges

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3D打印技术

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现代装饰（家居） 2015年第3期 46-51页

作者： Alessandro Zambelli Aud Julie Befring Gio Tirotto&Stefano Rigolli Linlin&Pierre-Yves Lanzavecchia+Wai marco Lafiandra Maria Jennifer Carew Studio MeraldiRubini Serena Confalonieri Selvaggia Armani 3 DOTS COLLECTIVE 伏平范嘉苑

上世纪70年代,随着3D辅助设计的兴起,设计师能在电脑软件中看到虚拟的三维物体,但要将这些物体用粘土、木头或金属做成模型却非常不容易。3D打印技术的出现,令平面变成立体的过程一下子简单了很多,设计师的任何改动都可在短时间内重新... 详细信息

上世纪70年代,随着3D辅助设计的兴起,设计师能在电脑软件中看到虚拟的三维物体,但要将这些物体用粘土、木头或金属做成模型却非常不容易。3D打印技术的出现,令平面变成立体的过程一下子简单了很多,设计师的任何改动都可在短时间内重新打印出来,而不用花几周的时间等着工厂将新模型制造出来,大大缩短了制作周期,降低了制作成本。并且,随着科技的不断进步,更多材料的产品可以打印出来。

关键词：三维物体花几制作成本制作周期电脑软件三维设计快速成型技术这个世界加成型小球体

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纤维板生产厂附近的儿童中室外甲醛和二氧化氮暴露与遗传毒性标志物

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环境与职业医学 2014年第9期31卷 682-682页

作者： Alessandro marcon Maria Enrica Fracasso Pierpaolo Marchetti Denise Doria Paolo Girardi Linda Guarda Giancarlo Pesce Vanda Pironi Paolo Ricci Roberto de marco

[背景]工业空气污染是一种公共卫生危害。以前的证据表明,在意大利最大的纤维板生产区（Viadana）工厂附近生活的儿童中呼吸道症状和住院率升高。[目的]随机选取住在Viadana附近的儿童（6~12岁）,评估户外甲醛和二氧化氮（NO2）暴露与... 详细信息

[背景]工业空气污染是一种公共卫生危害。以前的证据表明,在意大利最大的纤维板生产区（Viadana）工厂附近生活的儿童中呼吸道症状和住院率升高。[目的]随机选取住在Viadana附近的儿童（6~12岁）,评估户外甲醛和二氧化氮（NO2）暴露与口腔黏膜细胞中早期遗传毒性损伤标志物之间的关联。[方法]在2010—2011年,用彗星和微核实验分别评价口腔脱落细胞中的DNA链断裂和核异常,并用被动采样监测甲醛和NO2。通过空间插值法将每年的污染物暴露估计值分配到每个孩子的住所。[结果]在656名儿童中,有413名（63%）参加了研究。住在纤维板厂附近（〈2 km）的孩子的甲醛和NO2暴露平均水平最高（P〈0.001）。甲醛水平每增加1个标准差（0.20μg/m3）与彗尾强度升高0.13%（95%CI：0.03~0.22）、尾矩升高0.007（95%CI：0.001~0.012）和核芽相对增加12%[相对危险度（RR）=1.12;95%CI：1.02~1.23]有关联。NO2水平每增加一个标准差（2.13μg/m3）与双核细胞增加0.13%（95%CI：0.07~0.19）和核芽相对增加16%（RR=1.16,95%CI：1.06~1.26）有关联。[结论]在纤维板生产区域附近生活的儿童,其污染物暴露与口腔脱落细胞遗传毒性的标志物有关联。将这些研究结果与先前报道的纤维板生产活动与儿童呼吸系统结局间的关联相结合,加剧了人们对Viadana地区企业生产相关暴露所致潜在不利健康影响的担忧。

关键词：纤维板生产遗传毒性口腔黏膜细胞核芽脱落细胞微核实验毒性损伤采样监测双核细胞空气污染

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