检索结果-南通市图书馆

Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli

引用

Synthetic and Systems Biotechnology 2023年第4期8卷 629-639页

作者： Karel Olavarria marco v.becker Diana Z.Sousa Mark C.M.van Loosdrecht S.Aljoscha Wahl Laboratory of Microbiology Wageningen University and ResearchStippenenweg 46708 WEWageningenThe Netherlands Centre for Living Technologies Eindhoven-Wageningen-Utrecht AlliancePrincetonlaan 63584 CBUtrechtThe Netherlands Department of Biotechnology Applied Sciences FacultyDelft University of Technologyvan der Maasweg 92629 HZDelftThe Netherlands Lehrstuhl für Bioverfahrenstechnik Friedrich-Alexander-Universit¨atPaul-Gordan-Strasse 391052ErlangenGermany

Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages,such as increased yields and reduced energy ***,lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic *** stoichiometric analyses and genetic modifications are frequently employed in metabolic engineering,the use of thermodynamic tools to evaluate the feasibility of planned interventions is less *** this study,we propose a novel metabolic engineering strategy to achieve an efficient anaerobic production of poly-(R)-3-hydroxybutyrate(PHB)in the model organism Escherichia *** approach involves re-routing of two-thirds of the glycolytic flux through non-oxidative glycolysis and coupling PHB synthesis with NADH *** complemented our stoichiometric analysis with various thermodynamic approaches to assess the feasibility and the bottlenecks in the proposed engineered *** to our calculations,the main thermodynamic bottleneck are the reactions catalyzed by the acetoacetyl-CoAβ-ketothiolase(EC 2.***.1.9)and the acetoacetyl-CoA reductase(EC 1.***.1.36).Furthermore,we calculated thermodynamically consistent sets of kinetic parameters to determine the enzyme amounts required for sustaining the conversion *** the case of the engineered conversion route,the protein pool necessary to sustain the desired fluxes could account for 20%of the whole cell dry weight.

关键词： Pathway feasibility analysis Protein cost Anaerobic metabolism Engineered pathways Metabolite concentrations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optical probing of ultrafast laser-induced solid-to-overdense-plasma transitions

引用

Light(Science & Applications) 2024年第5期13卷 973-984页

作者： Yasmina Azamoum Georg Alexander becker Sebastian Keppler Guillaume Duchateau Stefan Skupin Mickael Grech Fabrice Catoire Sebastian Hell Issa Tamer marco Hornung marco Hellwing Alexander Kessler Franck Schorcht Malte Christoph Kaluza GSI Helmholtzzentrum für Schwerionenforschung Planckstr.164291 DarmstadtGermany. Helmholtz Institute Jena Fröbelstieg 307743 JenaGermany. Institute of Optics and Quantum Electronics Friedrich-SchillerUniversität JenaMax-Wien-Platz 107743 JenaGermany. CEA-CESTA 15 Avenue des SablièresCS6000133116 Le BarpCedexFrance Institut Lumière Matière UMR 5306-CNRSUniversitéde Lyon 169622 VilleurbanneFrance. LULI CNRSCEASorbonne UniversitéInstitut Polytechnique de ParisPalaiseauFrance. Universitéde Bordeaux-CNRS-CEA CELIAUMR 5107 TalenceFrance

Understanding the solid target dynamics resulting from the interaction with an ultrashort laser pulse is a challenging fundamental multi-physics problem involving atomic and solid-state physics,plasma physics,and laser *** of the initial interplay of the underlying processes is essential to many applications ranging from lowpower laser regimes like laser-induced ablation to high-power laser regimes like laser-driven ion *** the properties of the so-called pre-plasma formed as the laser pulse’s rising edge ionizes the target is complicated from the theoretical and experimental point of view,and many aspects of this laser-induced transition from solid to overdense plasma over picosecond timescales are still open *** the one hand,laser-driven ion acceleration requires precise control of the pre-plasma because the efficiency of the acceleration process crucially depends on the target properties at the arrival of the relativistic intensity peak of the *** the other hand,efficient laser ablation requires,for example,preventing the so-called“plasma shielding”.By capturing the dynamics of the initial stage of the interaction,we report on a detailed visualization of the pre-plasma formation and ***-thin diamond-like carbon foils are shown to transition from solid to plasma during the laser rising edge with intensitiesvidence sub-picosecond dynamics of an expanding plasma with densities above 10^(23)cm^(−3)(about 100 times the critical plasma density).The complementarity of a solid-state interaction model and kinetic plasma description provides deep insight into the interplay of initial ionization,collisions,and expansion.

关键词： laser acceleration solid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Factors influencing the drug release from calcium phosphate cements

引用

Bioactive Materials 2022年第1期7卷 341-363页

作者： marco Fosca Julietta v.Rau vuk Uskokovi Istituto di Struttura della Materia Consiglio Nazionale delle Ricerche(ISM-CNR)Via del Fosso del Cavaliere 10000133RomeItaly I.M.Sechenov First Moscow State Medical University Institute of PharmacyDepartment of AnalyticalPhysical and Colloid ChemistryTrubetskaya 8build.2119991MoscowRussia Advanced Materials and Nanobiotechnology Laboratory TardigradeNano LLCIrvineCA 92604United States

Thanks to their biocompatibility,biodegradability,injectability and self-setting properties,calcium phosphate cements(CPCs)have been the most economical and effective biomaterials of choice for use as bone void *** have also been extensively used as drug delivery carriers owing to their ability to provide for a steady release of various organic molecules aiding the regeneration of defective bone,including primarily antibiotics and growth *** review provides a systematic compilation of studies that reported on the controlled release of drugs from CPCs in the last 25 *** chemical,compositional and microstructural characteristics of these systems through which the control of the release rates and mechanisms could be achieved have been *** doing so,the effects of(i)the chemistry of the matrix,(ii)porosity,(iii)additives,(iv)drug types,(v)drug concentrations,(vi)drug loading methods and(vii)release media have been distinguished and discussed *** specificities of in vivo release of drugs from CPCs have been reviewed,*** the kinetic and mechanistic correlations between the CPC properties and the drug release is a prerequisite for the design of bone void fillers with drug release profiles precisely tailored to the application area and the clinical *** goal of this review has been to shed light on these fundamental correlations.

关键词： Calcium phosphate cements Drug release Release rate Antibiotics release Bone fillers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanical Properties of Sustainable,Self-healing Porous Asphalt Concrete

引用

武汉理工大学学报 2010年第17期32卷 22-25页

作者： LIU Q T ERIK SCHLANGEN MARTIN v D v marco POOT Micromechanics Laboratory Delft University of Technology2628 CN Delftthe Netherlands Road and Railway Engineering Delft University of TechnologyCiTG2628 CN Delftthe Netherlands

Long lifetime sustainable porous asphalt concrete containing steel fibers was designed. The material is self healing in such a sense that some external stimulus is needed in the form of heating by induction energy. Steel wool was added to porous asphalt concrete to enhance its electrical conductivity and induction heating was applied to increase the temperature to heal the micro-cracks and repair the bonding between aggregate and binder. The main purpose of this paper is to examine the mechanical properties of this sustainable porous asphalt concrete including indirect tensile strength,work of fracture,particle loss resistance and water sensitivity. It is found that adding steel fibers to porous asphalt concrete to increase the healing performance also can improve its overall mechanical properties.

关键词： conductive porous asphalt concrete steel fibers indirect tensile strength particle loss water sensitivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hybrid Triboelectric-Electromagnetic Nanogenerators for Mechanical Energy Harvesting:A Review

引用

Nano-Micro Letters 2021年第12期13卷 163-220页

作者： Joao v.vidal vladislav Slabov Andrei L.Kholkin marco P.Soares dos Santos Department of Physics and CICECO-Aveiro Institute of Materials University of Aveiro3810-193 AveiroPortugal Department of Physics and I3N University of Aveiro3810-193 AveiroPortugal Laboratory of Functional Low-Dimensional Structures National University of Science and Technology MISISMoscowRussia Department of Mechanical Engineering Centre for Mechanical Technology and Automation(TEMA)University of Aveiro3810-193 AveiroPortugal

Motion-driven electromagnetictriboelectric energy generators(E-TENGs) hold a Hybrid triboelectric-electromagnetic generators C A Bgreat potential to provide higher voltages,higher currents and wider operating bandwidths than Cantilever generators1 μm both electromagnetic and triboelectric generators Rotating generatorsSliding generators standing alone. Therefore,they are promising solutions to autonomously supply a broad range of Flexible blade generators Magnetoelectric highly sophisticated devices. This paper provides generators a thorough review focused on major recent breakLinear generators Multidimensional generators throughs in the area of electromagnetic-triboelectric vibrational energy harvesting. A detailed Pendulum generators analysis was conducted on various architectures including rotational,pendulum,linear,sliding,cantilever,flexible blade,multidimensional and 1 cm magnetoelectric,and the following hybrid technologies. They enable highly e cient ways to harvest electric energy from many forms of vibrational,rotational,biomechanical,wave,wind and thermal sources,among others. Open-circuit voltages up to 75 v,short-circuit currents up to 60 mA and instantaneous power up to 144 mW were already achieved by these nanogenerators. Their transduction mechanisms,including proposed models to make intelligible the involved physical phenomena,are also overviewed here. A comprehensive analysis was performed to compare their respective construction designs,external excitations and electric outputs. The results highlight the potential of hybrid E-TENGs to convert unused mechanical motion into electric energy for both large-and small-scale ***,this paper proposes future research directions toward optimization of energy conversion e ciency,power management,durability and stability,packaging,energy storage,operation input,research of transduction mechanisms,quantitative standardization,system integration,miniaturization and multi-energy hybrid cells.

关键词： E-TENG Hybrid triboelectric-electromagnetic Nanogenerators Energy harvesting

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Lower limb suspension induces threshold-specific alterations of motor units properties that are reversed by active recovery

引用

Journal of Sport and Health Science 2024年第2期13卷 264-276页

作者： Giacomo valli Fabio Sarto Andrea Casolo Alessandro Del vecchio Martino v.Franchi marco v.Narici Giuseppe De vito Department of Biomedical Sciences University of PadovaPadova 35131Italy Department Artificial Intelligence in Biomedical Engineering Friedrich-Alexander UniversityErlangen-Nurnberg 91052Germany

Purpose:This study aimed to non-invasively test the hypothesis that(a) short-term lower limb unloading would induce changes in the neural control of force production(based on motor units(MUs) properties) in the vastus lateralis muscle and(b) possible changes are reversed by active recovery(AR).Methods:Ten young males underwent 10 days of unilateral lower limb suspension(ULLS) followed by 21 days of *** ULLS,participants walked exclusively on crutches with the dominant leg suspended in a slightly flexed position(15°-20°) and with the contralateral foot raised by an elevated *** AR was based on resistance exercise(leg press and leg extension) and executed at 70% of each participant’s 1repetition maximum,3 times/*** voluntary isometric contraction(MvC) of knee extensors and MUs properties of the vastus lateralis muscle were measured at baseline,after ULLS,and after *** were identified using high-density electromyography during trapezoidal isometric contractions at 10%,25%,and 50% of the current MvC,and individual MUs were tracked across the 3 data collection ***:We identified 1428 unique MUs,and 270 of them(18.9%) were accurately *** ULLS,MvC decreased by 29.77%,MUs absolute recruitment/derecruitment thresholds were reduced at all contraction intensities(with changes between the 2 variables strongly correlated),while discharge rate was reduced at 10% and 25% but not at 50% *** MvC and MUs properties fully recovered to baseline levels after *** changes were observed in the pool of total as well as tracked ***:Our novel results demonstrate,non-invasively,that 10 days of ULLS affected neural control predominantly by altering the discharge rate of lower-threshold but not of higher-threshold MUs,suggesting a preferential impact of disuse on motoneurons with a lower depolarization ***,after 21 days of AR,the impaired MUs properties were fully restored to baseline levels,highlighting the plas

关键词： Disuse High-density EMG Muscle disuse Neural impairment Neuromuscular degeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Laparoscopic approach to gastrointestinal malignancies: Toward the future with caution

引用

World Journal of Gastroenterology 2014年第7期20卷 1777-1789页

作者： Lapo Bencini marco Bernini marco Farsi Division of Surgical Oncology Department of OncologyCareggi University Hospital50134 FlorenceItaly

After the rapid acceptance of laparoscopy to manage multiple benign diseases arising from gastrointestinal districts,some surgeons started to treat malignancies by the same ***,if the limits of laparoscopy for benign diseases are mainly represented by technical issues,oncologic outcomes remain the foundation of any procedures to cure *** patients represent an important group with peculiar aspects including reduced survival expectancy,worsened quality of life due to surgery itself and adjuvant therapies,and challenging psychological *** these issues could,potentially,receive a better management with a laparoscopic surgical *** order to confirm such aspects,similarly to testing the newest weapons(surgical or pharmacologic)against cancer,long-term follow-up is always recommendable to assess the real benefits in terms of overall survival,cancer-free survival and quality of ***,it seems of crucial importance that surgeons will be correctly trained in specific oncologic principles of surgical oncology as well as in modern miniinvasive ***,laparoscopic treatment of gastrointestinal malignancies requires more caution and deep analysis of published evidences,as compared to those achieved for inflammatory bowel diseases,gastroesophageal reflux disease or diverticular *** review tries to examine the evidence available to date for the use of laparoscopy and robotics in malignancies arising from the gastrointestinal district.

关键词： Gastrointestinal cancer Laparoscopic Oncology Laparoscopic surgery Robotic surgery Surgical outcomes Oncologic outcomes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Overlapping approach Proton Pump Inhibitors/Nux vomica-Heel as new intervention for gastro-esophageal reflux management:Delphi consensus study

引用

World Journal of Gastroenterology 2024年第18期30卷 2467-2478页

作者： Edda Battaglia Luciano Bertolusso marco Del Prete marco Monzani marco Astegiano Gastroenterology Unit ASLTO4Ivrea 10015TurinItaly General Practitioner ASL CN2Alba-Bra 12051CuneoItaly Specialist in Nephrology International Academy of Physiological Regulating Medicine Milan 20100Italy Specialist in Endocrinology Territorial Social and Health Authority of BrianzaMonza 20900Italy Gastroenterology Specialist Retired from Gastroenterology and Hepatology UnitAOU Cittàdella Salute e della ScienzaTurin 10123Italy

BACKGROUND Gastro-esophageal reflux disease(GERD)may affect the upper digestive tract;up to 20%of population in Western nations are affected by ***,histamine H2-receptor antagonists,and Proton Pump Inhibitors(PPIs)are considered the referring medications for ***,PPIs must be managed carefully because their use,especially chronic,could be linked with some adverse *** effective and safe alternative pharmacological tool for GERD is *** the identification of potentially new medications to flank PPIs,it is mandatory to revise and improve good clinical practices even through a consensus *** To optimize diagnosis and treatment guidelines for GERD through a consensus based on Delphi *** The availability of clinical studies describing the action of the multicomponent/multitarget medication Nux vomica-Heel,subject of the consensus,is the basic prerequisite for the consensus itself.A modified Delphi process was used to reach a consensus among a panel of Italian GERD specialists on the overlapping approach PPIs/Nux vomica-Heel as a new intervention model for the management of *** voting Consensus group was composed of 49 Italian Medical Doctors with different specializations:Gastroenterology,otolaryngology,geriatrics,and general medicine.A scientific committee analyzed the literature,determined areas that required investigation(in agreement with the multiple-choice questionnaire results),and identified two topics of interest:(1)GERD disease;and(2)GERD *** for each of these topics were then formulated and *** Delphi process involved two rounds of questioning submitted to the panel experts using an online *** According to their routinary GERD practice and current clinical evidence,the panel members provided feedback to each questionnaire *** experts evaluated 15 statements and reached consensus on all *** statements regarding the GERD disease showed high levels of

关键词： Gastro-esophageal reflux disease Proton Pump Inhibitors Consensus Nux vomica-Heel Overlapping Decalage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Benefits of conservation-driven mowing for the EU policy species Gladiolus palustris Gaudin in mountain fen meadows:a case-study in the European Alps

引用

Journal of Mountain Science 2020年第9期17卷 2097-2107页

作者： marco CANELLA Gianni POLONIATO Cesare LASEN Simone ORSENIGO Graziano ROSSI Jonas v.MüLLER Thomas ABELI Department of Earth and Environmental Sciences University of PaviaVia S.Epifanio1427100 PaviaItaly Dolomiti Bellunesi National Park Piazzale Zancanaro1-32032 FeltreItaly Millennium Seed Bank Conservation ScienceRoyal Botanic Gardens KewArdingly RH176TNWest SussexUK Department of Science University of Roma 3Viale Guglielmo Marconi 44600146RomaItaly

The sword lily Gladiolus palustris Gaudin is protected on European level and listed in Annexes II and Iv of the EC Habitat Directive 92/43/*** grows in nutrient-poor,calcareous meadows in central and eastern *** encroachment in montane meadows of the European Alps as a result of recent land use changes and the abandonment of traditional farming practices threaten the survival of this ***-driven mowing is considered a feasible conservation measure for maintaining high species diversity in abandoned semi-natural *** assess the effects of tenyears of biennial mowing on a grassland community in the Dolomiti Bellunesi National Park,Italy(Site of Community Importance,Natura 2000 network),ten25 m2 plots were established whereby four plots were placed in the mowed area,four in the non-mowed area and two in a small non-mowed patch of grassland inside the mowed *** each plot the following variables were recorded,total percentage of plant cover,percentage cover of woody species,percentage cover of herbaceous species,percentage cover and number of flowering ramets of *** and a complete list of species and their percentage *** plots showed a higher species richness than non-mowed *** number of *** flowering ramets and percentage cover increased manifold in mowed plots compared to nonmowed *** resumption of mowing forconservation purposes undertaken by the managing authority halted the process of tree encroachment and avoided a drastic change in plant *** mowing(every second or third year)was demonstrated to be a cost-effective conservation measure in non-productive grasslands to keep grasses at bay in favour of forbs of high conversation value.

关键词： Conservation management Seminatural habitats Habitat directive Montane grassland Natura 2000 network Dolomiti Bellunesi National Park

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Automated Synthesis of Wideband Bandpass Filters Based on Slow-Wave EBG Structures

引用

Computers, Materials & Continua 2016年第6期52卷 159-174页

作者： marco Orellana Jordi Selga Paris vélez Marc Sans Ana Rodríguez vicente Boria Ferran Martín GEMMA/CIMITEC Departament d’Enginyeria ElectrònicaUniversitat Autònoma de Barcelona08193 Bellaterra(Barcelona)Spain Departamento de Comunicaciones-iTEAM Universitat Politècnica de València46022 ValenciaSpain

This paper is focused on the automated synthesis of wideband bandpass filters operating at microwave frequencies and based on electromagnetic bandgap(EBG)*** classical counterpart of such filter consists of a combination of transmission line sections and shunt-connected grounded stubs placed at equidistant *** replacing the transmission line sections with capacitively-loaded lines(a kind of EBG-based lines)exhibiting the same phase shift at the lower cutoff frequency and the same characteristic(actually Bloch)impedance,filter size is reduced and the spurious pass bands can be efficiently *** practice,the loading capacitances are implemented by means of patches,in order to achieve a fully planar filter *** presence of the patches reduces the effective phase velocity of the capacitively-loaded lines,thus providing a slow-wave effect useful for filter ***,due to periodicity,such EBG-based lines exhibit wide stop bands,which are used for spurious *** though such EBGbased filters were previously reported by some of the authors,a systematic synthesis method was not applied for filter *** this paper,the main aim is to demonstrate the potential of aggressive space mapping(ASM)for that purpose,and it will be shown that such filters can be automatically synthesized.

关键词： Electromagnetic bandgaps(EBG) microwave filters slow-wave transmission lines space mapping.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：