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Ultrastructural Analysis of in Vitro Adherence and Production of Acid Proteases by Clinical Isolates of <i>Candida parapsilosis</i>Sensu Stricto Following Growth in the Presence of Keratinous Substrates from Human Source

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Advances in Microbiology 2013年第8期3卷 1-6页

作者： Ana Flávia L. Specian Luciana furlaneto-Maia Célia G. T. J. Andrade marcia cristina furlaneto Department of Microbiology Paraná State University at Londrina Londrina Brazil Electronic Microscopy and Microanalysis Laboratory Paraná State University at Londrina Londrina Brazil Technological Federal University of Paraná Londrina Brazil

Candida parapsilosis is an increasingly important human pathogen. However, little is known about its potential to cause disease. The aims of the present study were to analyse the production of acid proteinases by clinical isolates of C. parapsilosis in the presence of different keratinous substrates from human sources (stratum corneum, nail and hair) and to verify the capability of yeast cells to adhere and grow as biofilm on these substrates. By scanning electron microscopy, it was observed that all C. parapsilosis sensu stricto isolates adhered to the keratinous substrates. For the isolate recovered from onychomycosis, the cell population attached to stratum corneum and hair keratin consisted mainly of blastoconidia. Differently, on nail keratin, pseudohyphae production was observed. Overall, there was a loose association between yeast cells and keratinous substrates. However, on stratum corneum, flocculent extracellular material was seen evolving cells from the onychomycosis isolate by forming a biofilm-like structure. The isolates recovered from onychomycosis and cutaneous lesion produced higher amount of acid proteinases in medium supplemented with nail keratin and stratum corneum keratin, respectively, than that in salt medium (absence of keratin). Furthermore, no differences were observed in the amount of acid proteinases produced by the isolate recovered from tracheal secretion in the media tested (absence and presence of keratin substrates). The information derived from this study will further our understanding of acid proteinase production by C. parapsilosis isolates and provide an insight into pathogenic mechanisms in C. parapsilosis particularly from isolates recovered from superficial mycoses.

关键词： Adherence Keratinic Substrates SEM Acid Proteinase

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Challenges in Implementing DVI Best Practice Guidelines Due to the Brumadinho Disaster

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Forensic Medicine and Anatomy Research 2024年第1期12卷 1-12页

作者： Reinaldo Moreira Silva Bruno Diniz Sander Morais Carlos Danilo Ruas Silva Elza cristina Lopes de Oliveira Fernando Pereira Macedo Giovanni Vitral Pinto Guadalupe Dell’Arret Coutinho Higgor Gonçalves Dornelas João Luiz Silva Moreira Laila Evangelista de Almeida Fernandes marcia Lobão Iannini Patrícia Siqueira Valéria Rosalina Dias e Santos Sordaini Maria Caligiorne Instituto de Criminalística Polícia Civil de Minas Gerais Belo Horizonte Brazil

The broke of a tailings dam from Vale S.A. destroyed a gigantic area killing 270 persons in Brumadinho/Brazil. Organizing activities after a mass disaster is a complex process that requires the involvement of many people and resources in the laboratory. To DNA identification of the victims, a daunting work had to be made by DNA laboratory staff in a collaborative effort with many partners. Efforts to implement good practice guidelines in Disaster Victim Identification (DVI) have revealed several important aspects that need to change in the forensic DNA laboratory. This article highlights the challenges of implementing DVI best practice guidelines in resource-poor settings, but with professionals from different sectors engaged in the same goal of briefly identifying victims and helping families and society.

关键词： Disaster Victim Identification DNA Brumadinho

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Oxidative dehydrogenation of propane under steady-state and transient regimes over alumina-supported catalysts prepared from mixed V_2W_4O_(19)^(4-) hexametalate precursors

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Journal of Natural Gas Chemistry 2010年第2期19卷 123-133页

作者： Sébastien Paul Elise Berrier marcia cristina Kaezer Frana Jean-Guillaume Eon Univ Lille Nord de France F-59000 LilleFrance ECLille UCCSF-59650 Villeneuve d'AscqFrance CNRS UMR 8181F-59650 Villeneuve d'AscqFrance USTL UCCSF-59650 Villeneuve d'AscqFrance Instituto de Quimíca Universidade Federal do Rio de Janeiro-Cidade UniversitáriaIlha do Fundo21945-970 Rio de JaneiroBrazil

An integrated approach combining the development of an innovative catalyst and the research of a set of adequate operating conditions for the propane oxidative dehydrogenation （ODH） is *** experimental set-up,specially designed for steady-state and transient studies is *** preparation method,the characterization and the performances in steady-state and transient regimes of catalysts based on V2W4O194-Lindqvist isopolyanion used as a precursor and supported on alumina are *** influence of the preparation method of the catalyst and the role of water in the feed gas are more particularly discussed.

关键词： propane oxidative dehydrogenation (ODH) transient regime pulsed reactor Lindqvist isopolyanion in-situ Raman

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Poly(Butylene Adipate-Co-Terephthalate)and Poly(ε-Caprolactone)and Their Bionanocomposites with Cellulose Nanocrystals:Thermo-Mechanical Properties and Cell Viability Study

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Journal of Renewable Materials 2019年第3期7卷 269-277页

作者： marcia cristina Branciforti Caroline Faria Bellani Carolina Lipparelli Morelli Alice Ferrand Nadia Benkirane-Jessel Rosario Elida Suman Bretas Departamento de Engenharia de Materiais Universidade de Sao PauloSao CarlosSPBrasil Departamento de Engenharia de Materiais Universidade Federal de Sao CarlosSao CarlosSPBrasil Institut National de la Santéet de la Recherche Médicale Universitéde StrasbourgStrasbourgFrance

Although nanocomposites have recently attracted special interest in the tissue engineering area,due to their potential to reinforce scaffolds for hard tissues applications,a number of variables must be set prior to any clinical *** manuscript addresses the evaluation of thermo-mechanical properties and of cell proliferation of cellulose nanocrystals(CNC),poly(butylene adipate-co-terephthalate)(PBAT),poly(ε-caprolactone)(PCL)films and their bionanocomposites with 2 wt% of CNC obtained by casting *** nanocrystals extracted from Balsa wood by acid hydrolysis were used as a reinforcing phase in PBAT and PCL matrix *** films and pure CNC at different concentrations were cultured with osteoblasts MG-63 and the cell proliferation was assessed by AlamarBlue?*** thermal-mechanical properties of the films were evaluated by dynamic-mechanical thermal analysis(DMTA).It was found by DMTA that the CNC acted as reinforcing *** addition of CNCs in the PBAT and PCL matrices induced higher storage moduli due to the reinforcement effects of *** cell viability results showed that neat CNC favored osteoblast proliferation and both PBAT and PCL films incorporated with CNC were biocompatible and supported cell proliferation along *** nature of the polymeric matrix or the presence of CNC practically did not affect the cell proliferation,confirming they have no in vitro *** features make cellulose nanocrystals a suitable candidate for the reinforcement of biodegradable scaffolds for tissue engineering and biomedical applications.

关键词： Cell viability thermo-mechanical properties cellulose nanocrystals(CNC) biocompatible polymers tissue engineering bionanocomposites

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Why Brazilian Women Are Not on Top： The Work-Life Reconciliation Hypothesis

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Chinese Business Review 2017年第1期16卷 47-53页

作者： marcia cristina Esteves Agostinho University Estacio de Sa Rio de Janeiro Brazil

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Nutritional,rheological and sensory properties of butter processed with different mixtures of cow and sheep milk cream

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Food Bioscience 2022年第2期46卷 487-497页

作者： Renata S Dias Celso F.Balthazar Rodrigo N.Cavalcanti Louise A.Sobral Jessica F.Rodrigues Roberto P.CNeto Maria Inˆes B.Tavares Ana Paula B.Ribeiro Renato Grimaldi Celso Sant’Anna Erick A.Esmerino Tatiana C.Pimentel marcia cristina Silva Adriano G.Cruz Instituto Federal de Educaç˜ao Ciˆencia e Tecnologia do Rio de Janeiro(IFRJ)Departamento de AlimentosRJBrazil Universidade Estadual de Campinas(UNICAMP) Faculdade de Engenharia de AlimentosCampinasSPBrazil Universidade Federal do Rio de Janeiro(UFRJ) Escola de QuimicaRJBrazil Instituto Federal of Minas Gerais(IFMG) Departamento de Ciˆencias Agr´ariasBambuíMGBrazil Universidade Federal do Rio de Janeiro(UFRJ) Instituto de Macromol´eculas Professora Eloisa Mano(IMA)Rio de JaneiroBrazil Instituto Federal do Paran´a(IFPR) ParanavaíPRBrazil g Instituto Nacional de MetrologiaNormalizaç˜ao Qualidade Industrial(affnInmetro) Duque de Caxias25250-020Rio de JaneiroBrazil Universidade Federal Fluminense(UFF) Faculdade de Medicina VeterinariaNiter´oiRJBrazil

This study assessed the quality parameters and consumer perception and behavior of butter processed with different ratios of cow and sheep milk *** milk cream butter(CMCB)showed lower moisture content and higher fat content,smaller fat globules,and higher concentrations of long-chain saturated fatty acids and monounsaturated fatty acids,resulting in improved potential health *** addition,CMCB showed yellow color,little salt,and butter taste as the main sensory characteristics,resulting in grateful and good digestibility *** milk cream addition improved the rheological properties of the products(lower viscosity,consistency index,and rigidity(J0),and higher fluidity and viscoelastic behavior(J1)),mainly due to the increased concentrations of short-chain and medium-chain saturated fatty acids and polyunsaturated fatty ***,sheep milk cream butter(SMCB)was characterized by a greasy and striking aroma,with a lower perception of good digestibility and good appearance,and a higher bitter *** association of cow and sheep milk cream could be an exciting option to obtain products with improved fatty acid profiles,health indices,and yet maintain desired rheological and sensory *** formulation with 60%sheep milk cream and 40%cow milk cream would be the suggested combination.

关键词： Sheep milk Bovine milk Quality parameters Butter

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Dewetting in associating lattice gas model confined by hydrophobic walls

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Science China(Physics,Mechanics & Astronomy) 2019年第10期62卷 60-67页

作者： Tassylla OFonseca marcia M.Szortyka Patricia Temes cristina Gavazzoni Alan Bde Oliveira marcia CBarbosa Instituto de Fsica Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brasil Universidade Federal de Santa Catarina Rua Pedro Joao PereiraMato Alto 88900-000Brasil Instituto Federal Catarinense Sao Bento do Sul 89283-064Brasil Instituto de Quimica Universidade Estadual de CampinasCampinas 13083-970Brasil Departamento de Fisica Universidade Federal de Ouro PretoOuro Preto 35400-000Brasil

The phase behavior of a two dimensional fluid confined within hydrophobic walls is obtained by Monte Carlo *** fluid is described by the associating lattice gas model which reproduces the density and diffusion anomalous behavior of *** confined fluid exhibits a liquid-liquid critical temperature which decreases with the decrease of the distance between the confining *** contact with the wall a dewetting is *** thickness of this interfacial layer is independent of the distance between the two *** for very small distances between the two walls no total depletion is observed and consequently no drying transition is present.

关键词： water lattice confined

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Stem cells and tissue engineering: an alternative treatment for craniofacial congenital malformations and articular degenerative diseases

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Plastic and Aesthetic Research 2020年第6期7卷 24-37页

作者： cristina Velasquillo Antonio Madrazo-Ibarra Claudia Gutiérrez-Gómez marcia Rosario Pérez-Dosal Yaaziel Melgarejo-Ramírez Clemente Ibarra Biotecnología Instituto Nacional de Rehabilitación LGIICiudad de México 14389México Hospital General Manuel Gea González and Instituto Nacional de Rehabilitación LGII Ciudad de México 14080México Médico Adscrito al Servicio de Cirugía Plástica Instituto Nacional de PediatríaCiudad de México 04530México Dirección General Instituto Nacional de Rehabilitación LGIICiudad de México 14389México

The life quality of patients with craniofacial malformations is severely affected by the physical disabilities caused by the malformation itself,but also by being subjected to bullying,which leads to a series of relevant psychological and societal effects that have an economic impact on the health *** clefts,notably cleft lip(CL),cleft palate,and microtia,are the most common craniofacial birth defects in humans and represent a substantial burden,both personal and *** the other hand,osteoarthritis is a widespread degenerative disease that is becoming more common due to the extension of the human lifespan and to an increase in injuries in young people as a result of their *** in tissue engineering as a part of regenerative medicine offer new hope to patients that can benefit from new tissue engineering therapies based on the supportive action of tailored 3D biomaterials and the synergic action of stem cells that can be driven to the process of bone and cartilage *** review provides an update on recent considerations for stem cells and studies on the use of advanced biomaterials and cell therapies for the regeneration of craniofacial congenital malformations and articular degenerative diseases.

关键词： Mesenchymal stromal cells microtia cartilage cleft lip cleft palate

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The effects of neem [Azadirachta indica A. Juss (meliaceae)] oil on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans and the production of fusaric acid toxin

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Advances in Bioscience and Biotechnology 2010年第1期1卷 1-6页

作者： marcia Regina Ferreira Geraldo Carla cristina Arroteia Carlos Kemmelmeier 不详

Fungi of the genus Fusarium are well known plant pathogens, cause several vascular diseases and are producers of toxins. In vitro assays evaluated the effects of Neem (Azadirachta indica) oil on the diameter of colonies, dry weight, spore production, spore viability and production of Fusaric Acid toxin on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans isolates. Effects of Neem oil were analyzed at concentrations 0.25%, 0.5% and 1% in Czapek Yeast Agar medium. The production of Fu- saric acid was determined by Thin Layer Chromatography and quantified by UV spectrophotometry. Neem oil showed inhibitory effects on the isolates tested, although they varied according to type of isolate and oil concentration. Neem oil was efficient in reducing the colonies’ diameter and dry weight in concentration-dependent manner. Neem oil was efficacious at higher concentration in the decrease of sporulation. Spore germination was affected by Neem oil when the spore was grown in Neem-contained medium as when the spore emerged from a culture in a Neem medium. Neem oil decreased and even inhibited the production of Fusaric acid by the assayed isolates. Since these isolates are plant pathogens and producers of Fusaric acid, Neem oil may be introduced as an integral item in the management of host plants.

关键词： Fusarium Azadirachta Indica Fusaric Acid Antifungal Activity

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Polymorphisms of folate metabolism genes in patients with cirrhosis and hepatocellular carcinoma

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World Journal of Hepatology 2016年第29期8卷 1234-1243页

作者： Nathália Perpetua Peres Ana Lívia Silva Galbiatti-Dias marcia Maria Urbanin Castanhole-Nunes Renato Ferreira da Silva Erika cristina Pavarino Eny Maria Goloni-Bertollo Mariangela Torreglosa Ruiz-Cintra Genetics and Molecular Biology Research Unit UPGEM Sao Jose do Rio Preto Medical School FAMERP and Medical School Fundation FUNFARME Study Group of Liver Tumors GETF Hospital de Base Federal University of the Triangulo Mineiro UFTM

AIM To evaluated the association of the risk factors and polymorphisms in MTHFR C677 T, MTHFR A1298 C, MTR A2756 G and MTRR A66 G *** Patients with cirrhosis(n = 116), hepatocellular carcinoma(HCC)(n = 71) and controls(n = 356) were included. Polymerase chain reaction followed by enzymatic digestion and allelic discrimination technique real-time PCR techniques were used for analysis. MINITAB-14.0and SNPstats were utilized for statistical analysis. RESULTS Showed that age ≥ 46 years(OR = 10.31; 95%CI: 5.66-18.76; P < 0.001) and smoking(OR = 0.47; 95%CI: 0.28-0.78; P = 0.003) were associated with cirrhosis. Age ≥ 46 years(OR = 16.36; 95%CI: 6.68-40.05; P < 0.001) and alcohol habit(OR = 2.01; 95%CI: 1.03-3.89; P = 0.039) were associated with HCC. MTHFR A1298 C in codominant model(OR = 3.37; 95%CI: 1.52-7.50; P = 0.014), recessive model(OR = 3.04; 95%CI: 1.43-6.47; P = 0.0051) and additive model(OR = 1.71; 95%CI: 1.16-2.52; P = 0.0072) was associated with HCC, as well as MTR A2756 G in the additive model(OR = 1.68; 95%CI: 1.01-2.77; P = 0.047), and MTRR A66 G in the codominant model(OR = 3.26; 95%CI: 1.54-6.87; P < 0.001), dominant model(OR = 2.55; 95%CI: 1.24-5.25; P = 0.007) and overdominant model(OR = 3.05; 95%CI: 1.66-5.62; P < 0.001). MTR A2756 G in the additive model(OR = 1.54; 95%CI: 1.02-2.33; P = 0.042) and smokers who presented at least one polymorphic allele for MTRR A66G(OR = 1.71; 95%CI: 0.77-3.82; P = 0.0051) showed increased risk for cirrhosis. There was no association between clinical parameters and polymorphisms. CONCLUSION Age ≥ 46 years, alcohol habit and MTR A2756 G, MTHFR A1298 C and MTRR A66 G polymorphisms are associated with an increased risk of HCC development; age ≥ 46 years, tobacco habit and the MTR A2756 G polymorphism are associated with cirrhosis.

关键词： Polymorphism Folate metabolism Liver cirrhosis Hepatocellular carcinoma

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